Restriction map for pF1KB9635
 (Linear) (Six-base) MAPSORT of: KIBB9635.seq  Check: 4963  from: 1  to: 969



KIBB9635.orf

 With 182 enzymes: SgfI * 

                        June 25, 2015 11:41  ..

AccI GT'mk_AC

Cuts at:      0     253     969 
   Size:        253     716
 
AflIII A'CryG_T

Cuts at:      0     917     969 
   Size:        917      52
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     376     969 
   Size:        376     593
 
AlwNI CAG_nnn'CTG

Cuts at:      0     324     685     969 
   Size:        324     361     284

  Fragments arranged by size: 

                 361     324     284
 
ApaLI G'TGCA_C

Cuts at:      0     649     969 
   Size:        649     320
 
AvaI C'yCGr_G

Cuts at:      0     129     969 
   Size:        129     840
 
BamHI G'GATC_C

Cuts at:      0     733     969 
   Size:        733     236
 
BanI G'GyrC_C

Cuts at:      0     109     897     931     969 
   Size:        109     788      34      38

  Fragments arranged by size: 

                 788     109      38      34
 
BanII G_rGCy'C

Cuts at:      0      50     181     969 
   Size:         50     131     788

  Fragments arranged by size: 

                 788     131      50
 
BbeI G_GCGC'C

Cuts at:      0     935     969 
   Size:        935      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     682     969 
   Size:        682     287
 
BglII A'GATC_T

Cuts at:      0     524     969 
   Size:        524     445
 
BlpI GC'TnA_GC

Cuts at:      0     151     969 
   Size:        151     818
 
Bme1580I G_kGCm'C

Cuts at:      0     112     653     969 
   Size:        112     541     316

  Fragments arranged by size: 

                 541     316     112
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     113     870     969 
   Size:        113     757      99

  Fragments arranged by size: 

                 757     113      99
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     969 
   Size:        517     452
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     766     969 
   Size:        316     450     203

  Fragments arranged by size: 

                 450     316     203
 
BsaAI yAC'GTr

Cuts at:      0     918     969 
   Size:        918      51
 
BsaHI Gr'CG_yC

Cuts at:      0      11     932     962     969 
   Size:         11     921      30       7

  Fragments arranged by size: 

                 921      30      11       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     514     969 
   Size:        514     455
 
BseYI C'CCAG_C

Cuts at:      0     813     969 
   Size:        813     156
 
BsiEI CG_ry'CG

Cuts at:      0     752     806     969 
   Size:        752      54     163

  Fragments arranged by size: 

                 752     163      54
 
BsiHKAI G_wGCw'C

Cuts at:      0     653     969 
   Size:        653     316
 
BsiWI C'GTAC_G

Cuts at:      0     407     969 
   Size:        407     562
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     334     969 
   Size:        334     635
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     112     181     653     969 
   Size:         50      62      69     472     316

  Fragments arranged by size: 

                 472     316      69      62      50
 
BsrBI CCG'CTC

Cuts at:      0     845     969 
   Size:        845     124
 
BsrFI r'CCGG_y

Cuts at:      0      66     934     969 
   Size:         66     868      35

  Fragments arranged by size: 

                 868      66      35
 
BssHII G'CGCG_C

Cuts at:      0     188     463     969 
   Size:        188     275     506

  Fragments arranged by size: 

                 506     275     188
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     324     685     875     890     969 
   Size:        324     361     190      15      79

  Fragments arranged by size: 

                 361     324     190      79      15
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     495     544     969 
   Size:        495      49     425

  Fragments arranged by size: 

                 495     425      49
 
BstYI r'GATC_y

Cuts at:      0     524     733     969 
   Size:        524     209     236

  Fragments arranged by size: 

                 524     236     209
 
BtgI C'CryG_G

Cuts at:      0     380     644     704     800     969 
   Size:        380     264      60      96     169

  Fragments arranged by size: 

                 380     264     169      96      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     360     969 
   Size:        360     609
 
BtsI GCAGTG_nn'

Cuts at:      0     222     840     969 
   Size:        222     618     129

  Fragments arranged by size: 

                 618     222     129
 
DraIII CAC_nnn'GTG

Cuts at:      0     918     969 
   Size:        918      51
 
EaeI y'GGCC_r

Cuts at:      0     803     969 
   Size:        803     166
 
EagI C'GGCC_G

Cuts at:      0     803     969 
   Size:        803     166
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     969 
   Size:        517     452
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     394     969 
   Size:        394     575
 
FspI TGC'GCA

Cuts at:      0     430     969 
   Size:        430     539
 
HaeII r_GCGC'y

Cuts at:      0      98     158     173     722     935     969 
   Size:         98      60      15     549     213      34

  Fragments arranged by size: 

                 549     213      98      60      34      15
 
HindIII A'AGCT_T

Cuts at:      0     433     969 
   Size:        433     536
 
KasI G'GCGC_C

Cuts at:      0     931     969 
   Size:        931      38
 
MslI CAynn'nnrTG

Cuts at:      0     379     478     969 
   Size:        379      99     491

  Fragments arranged by size: 

                 491     379      99
 
MspA1I CmG'CkG

Cuts at:      0      89     324     646     682     697     706     802     813 
   Size:         89     235     322      36      15       9      96      11

Cuts at:    813     969 
   Size:        156

  Fragments arranged by size: 

                 322     235     156      96      89      36      15      11
                   9
 
NaeI GCC'GGC

Cuts at:      0      68     936     969 
   Size:         68     868      33

  Fragments arranged by size: 

                 868      68      33
 
NarI GG'CG_CC

Cuts at:      0     932     969 
   Size:        932      37
 
NcoI C'CATG_G

Cuts at:      0     380     969 
   Size:        380     589
 
NgoMIV G'CCGG_C

Cuts at:      0      66     934     969 
   Size:         66     868      35

  Fragments arranged by size: 

                 868      66      35
 
NotI GC'GGCC_GC

Cuts at:      0     803     969 
   Size:        803     166
 
NspI r_CATG'y

Cuts at:      0     363     873     891     969 
   Size:        363     510      18      78

  Fragments arranged by size: 

                 510     363      78      18
 
PmlI CAC'GTG

Cuts at:      0     918     969 
   Size:        918      51
 
PstI C_TGCA'G

Cuts at:      0     320     702     886     969 
   Size:        320     382     184      83

  Fragments arranged by size: 

                 382     320     184      83
 
PvuII CAG'CTG

Cuts at:      0     324     969 
   Size:        324     645
 
SacII CC_GC'GG

Cuts at:      0     647     707     803     969 
   Size:        647      60      96     166

  Fragments arranged by size: 

                 647     166      96      60
 
SfcI C'TryA_G

Cuts at:      0     316     698     882     969 
   Size:        316     382     184      87

  Fragments arranged by size: 

                 382     316     184      87
 
SfoI GGC'GCC

Cuts at:      0     933     969 
   Size:        933      36
 
SmlI C'TyrA_G

Cuts at:      0     331     781     969 
   Size:        331     450     188

  Fragments arranged by size: 

                 450     331     188
 
SphI G_CATG'C

Cuts at:      0     363     873     969 
   Size:        363     510      96

  Fragments arranged by size: 

                 510     363      96
 
StyI C'CwwG_G

Cuts at:      0     380     969 
   Size:        380     589
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     738     969 
   Size:        738     231
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     456     969 
   Size:        456     513
 

 Enzymes that do cut:

     AccI   AflIII     AlfI    AlwNI    ApaLI     AvaI    BamHI     BanI
    BanII     BbeI     BglI    BglII     BlpI Bme1580I     BmrI     BpmI
    BpuEI    BsaAI    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BsrBI    BsrFI   BssHII   BstAPI    BstXI    BstYI
     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI  Eco57MI    EcoNI
     FspI    HaeII  HindIII     KasI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NotI     NspI     PmlI     PstI    PvuII    SacII
     SfcI     SfoI     SmlI     SphI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaWI    BsaXI
     BsgI     BsmI    BspEI    BspHI    BspMI    BsrDI    BsrGI    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI
     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
    FspAI    Hin4I   HincII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     NdeI     NheI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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