Restriction map for pF1KB9520
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# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        AlwNI       AocI        ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbsI        
BcgI        BciVI       BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      Eam1105I    EarI        
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FseI        FspI        HindIII     HpaI        KpnI        
Ksp632I     MaeI        MfeI        MflI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tsp509I     
Tth111I     XbaI        XcmI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Alw44I        351  GTGCAC               G^TGCAC
Aor51HI      1037  AGCGCT               AGC^GCT
ApaLI         351  GTGCAC               G^TGCAC
Asp700I       204  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI          250  GGCGCC               G^G[CT][GA]CC
BbeI          250  GGCGCC               GGCGC^C
BclI         1133  TGATCA               T^GATCA
BsaAI         280  TACGTG               [CT]AC^GT[GA]
BsaBI        1131  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          417  GGTCTC               GGTCTC(1/5)
BsgI          814  CTGCAC               GTGCAG(16/14)
BspMI         344  ACCTGC               ACCTGC(4/8)
BsrBI         413  GAGCGG               CCGCTC(-3/-3)
BsrFI         718  GCCGGC               [GA]^CCGG[CT]
BstEII        405  GGTCACC              G^GT[ACGT]ACC
BstPI         405  GGTCACC              G^GT[ACGT]ACC
Cfr10I        718  GCCGGC               [GA]^CCGG[CT]
DpnII        1134  GATC                 ^GATC
DrdI          238  GACTTCCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1037  AGCGCT               AGC^GCT
EcoO65I       405  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1152  CCATGG               C^C[AT][AT]GG
FbaI         1133  TGATCA               T^GATCA
HincII       1219  GTCAAC               GT[CT]^[GA]AC
HindII       1219  GTCAAC               GT[CT]^[GA]AC
HinfI         184  GACTC                G^A[ACGT]TC
KasI          250  GGCGCC               G^GCGCC
KspI          101  CCGCGG               CCGC^GG
MaeII         281  ACGT                 A^CGT
MamI         1131  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI         1134  GATC                 ^GATC
MseI          650  TTAA                 T^TAA
NaeI          718  GCCGGC               GCC^GGC
NarI          250  GGCGCC               GG^CGCC
NcoI         1152  CCATGG               C^CATGG
NdeII        1134  GATC                 ^GATC
NgoMIV        718  GCCGGC               G^CCGGC
PflMI         760  CCACCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          184  GACTC                GAGTC(4/5)
SacII         101  CCGCGG               CCGC^GG
Sau3AI       1134  GATC                 ^GATC
SfoI          250  GGCGCC               GGC^GCC
StyI         1152  CCATGG               C^C[AT][AT]GG
Tru9I         650  TTAA                 T^TAA
Van91I        760  CCACCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          204  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AcyI          250  GGCGCC               G[GA]^CG[CT]C
              981  GGCGTC              
ApaI          428  GGGCCC               GGGCC^C
             1112  GGGCCC              
BsaHI         250  GGCGCC               G[GA]^CG[CT]C
              981  GGCGTC              
BseRI         357  CTCCTC               GAGGAG(10/8)
              927  CTCCTC              
BsmAI         418  GTCTC                GTCTC(1/5)
             1050  GTCTC               
BsrI          584  ACTGG                ACTGG(1/-1)
             1175  ACTGG               
BstXI         140  CCACGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1070  CCAAGTCGCTGG        
DraII         311  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1112  GGGCCCC             
EagI          306  CGGCCG               C^GGCCG
              839  CGGCCG              
EclXI         306  CGGCCG               C^GGCCG
              839  CGGCCG              
Eco52I        306  CGGCCG               C^GGCCG
              839  CGGCCG              
EcoO109I      311  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             1112  GGGCCCC             
Hin1I         250  GGCGCC               G[GA]^CG[CT]C
              981  GGCGTC              


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaI        AvaII       BalI        BanII       BbvCI       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaJI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BssHII      BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DsaI        EaeI        EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HpaII       
HphI        ItaI        MaeIII      MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       PaeR7I      RsaI        Sau96I      ScrFI       
SfaNI       SmlI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XhoI        
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