Restriction map for pF1KB9520
 (Linear) (Six-base) MAPSORT of: KIBB9520.seq  Check: 1979  from: 1  to: 1335



KIBB9520.orf

 With 182 enzymes: SgfI * 

                        November 2, 2010 17:11  ..

AfeI AGC'GCT

Cuts at:      0    1039    1335 
   Size:       1039     296
 
ApaI G_GGCC'C

Cuts at:      0     432    1116    1335 
   Size:        432     684     219

  Fragments arranged by size: 

                 684     432     219
 
ApaLI G'TGCA_C

Cuts at:      0     351    1335 
   Size:        351     984
 
AvaI C'yCGr_G

Cuts at:      0     186     410     550     707     724     736     871     930 
   Size:        186     224     140     157      17      12     135      59

Cuts at:    930    1052    1335 
   Size:        122     283

  Fragments arranged by size: 

                 283     224     186     157     140     135     122      59
                  17      12
 
BanI G'GyrC_C

Cuts at:      0     250    1335 
   Size:        250    1085
 
BanII G_rGCy'C

Cuts at:      0     432     708     731     970    1106    1116    1335 
   Size:        432     276      23     239     136      10     219

  Fragments arranged by size: 

                 432     276     239     219     136      23      10
 
BbeI G_GCGC'C

Cuts at:      0     254    1335 
   Size:        254    1081
 
BbvCI CC'TCA_GC

Cuts at:      0     634     655     851    1335 
   Size:        634      21     196     484

  Fragments arranged by size: 

                 634     484     196      21
 
BclI T'GATC_A

Cuts at:      0    1133    1335 
   Size:       1133     202
 
BglI GCCn_nnn'nGGC

Cuts at:      0      21     178     862    1213    1335 
   Size:         21     157     684     351     122

  Fragments arranged by size: 

                 684     351     157     122      21
 
Bme1580I G_kGCm'C

Cuts at:      0     355     432     797     940    1116    1335 
   Size:        355      77     365     143     176     219

  Fragments arranged by size: 

                 365     355     219     176     143      77
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634     897    1018    1335 
   Size:        634     263     121     317

  Fragments arranged by size: 

                 634     317     263     121
 
Bpu10I CC'TnA_GC

Cuts at:      0     514     634     655     851    1335 
   Size:        514     120      21     196     484

  Fragments arranged by size: 

                 514     484     196     120      21
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     943    1335 
   Size:        943     392
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     423    1335 
   Size:        423     912
 
BsaAI yAC'GTr

Cuts at:      0     282    1335 
   Size:        282    1053
 
BsaBI GATnn'nnATC

Cuts at:      0    1135    1335 
   Size:       1135     200
 
BsaHI Gr'CG_yC

Cuts at:      0     251     982    1335 
   Size:        251     731     353

  Fragments arranged by size: 

                 731     353     251
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     514     544    1171    1201    1335 
   Size:        514      30     627      30     134

  Fragments arranged by size: 

                 627     514     134      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     348     918    1335 
   Size:        348     570     417

  Fragments arranged by size: 

                 570     417     348
 
BseYI C'CCAG_C

Cuts at:      0     147     431     518     743     773    1034    1109    1335 
   Size:        147     284      87     225      30     261      75     226

  Fragments arranged by size: 

                 284     261     226     225     147      87      75      30
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1335 
   Size:        799     536
 
BsiEI CG_ry'CG

Cuts at:      0     309     393     842    1335 
   Size:        309      84     449     493

  Fragments arranged by size: 

                 493     449     309      84
 
BsiHKAI G_wGCw'C

Cuts at:      0     355     380    1325    1335 
   Size:        355      25     945      10

  Fragments arranged by size: 

                 945     355      25      10
 
Bsp1286I G_dGCh'C

Cuts at:      0     355     380     432     708     731     797     940     970 
   Size:        355      25      52     276      23      66     143      30

Cuts at:    970    1106    1116    1325    1335 
   Size:        136      10     209      10

  Fragments arranged by size: 

                 355     276     209     143     136      66      52      30
                  25      23      10      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     353    1335 
   Size:        353     982
 
BsrBI CCG'CTC

Cuts at:      0     415    1335 
   Size:        415     920
 
BsrFI r'CCGG_y

Cuts at:      0     718    1335 
   Size:        718     617
 
BssHII G'CGCG_C

Cuts at:      0       6       8      76    1335 
   Size:          6       2      68    1259

  Fragments arranged by size: 

                1259      68       6       2
 
BstEII G'GTnAC_C

Cuts at:      0     405    1335 
   Size:        405     930
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     147    1077    1335 
   Size:        147     930     258

  Fragments arranged by size: 

                 930     258     147
 
BtgI C'CryG_G

Cuts at:      0     101     140     896    1152    1335 
   Size:        101      39     756     256     183

  Fragments arranged by size: 

                 756     256     183     101      39
 
BtsI GCAGTG_nn'

Cuts at:      0     461     558    1158    1323    1335 
   Size:        461      97     600     165      12

  Fragments arranged by size: 

                 600     461     165      97      12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     244    1335 
   Size:        244    1091
 
EaeI y'GGCC_r

Cuts at:      0     110     137     179     306     839    1067    1079    1155 
   Size:        110      27      42     127     533     228      12      76

Cuts at:   1155    1335 
   Size:        180

  Fragments arranged by size: 

                 533     228     180     127     110      76      42      27
                  12
 
EagI C'GGCC_G

Cuts at:      0     306     839    1335 
   Size:        306     533     496

  Fragments arranged by size: 

                 533     496     306
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1246    1335 
   Size:       1246      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634     897    1018    1335 
   Size:        634     263     121     317

  Fragments arranged by size: 

                 634     317     263     121
 
EcoO109I rG'GnC_Cy

Cuts at:      0     312    1113    1335 
   Size:        312     801     222

  Fragments arranged by size: 

                 801     312     222
 
HaeII r_GCGC'y

Cuts at:      0      46     127     254     851    1041    1335 
   Size:         46      81     127     597     190     294

  Fragments arranged by size: 

                 597     294     190     127      81      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     494     514     526     546    1335 
   Size:        494      20      12      20     789

  Fragments arranged by size: 

                 789     494      20      20      12
 
HincII GTy'rAC

Cuts at:      0    1221    1335 
   Size:       1221     114
 
KasI G'GCGC_C

Cuts at:      0     250    1335 
   Size:        250    1085
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     260    1335 
   Size:        260    1075
 
MscI TGG'CCA

Cuts at:      0     139    1069    1157    1335 
   Size:        139     930      88     178

  Fragments arranged by size: 

                 930     178     139      88
 
MslI CAynn'nnrTG

Cuts at:      0     563     821    1012    1335 
   Size:        563     258     191     323

  Fragments arranged by size: 

                 563     323     258     191
 
MspA1I CmG'CkG

Cuts at:      0      27     103     296     813    1335 
   Size:         27      76     193     517     522

  Fragments arranged by size: 

                 522     517     193      76      27
 
NaeI GCC'GGC

Cuts at:      0     720    1335 
   Size:        720     615
 
NarI GG'CG_CC

Cuts at:      0     251    1335 
   Size:        251    1084
 
NcoI C'CATG_G

Cuts at:      0    1152    1335 
   Size:       1152     183
 
NgoMIV G'CCGG_C

Cuts at:      0     718    1335 
   Size:        718     617
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     766    1335 
   Size:        766     569
 
PspOMI G'GGCC_C

Cuts at:      0     428    1112    1335 
   Size:        428     684     223

  Fragments arranged by size: 

                 684     428     223
 
PspXI vC'TCGA_Gb

Cuts at:      0     186     930    1335 
   Size:        186     744     405

  Fragments arranged by size: 

                 744     405     186
 
SacII CC_GC'GG

Cuts at:      0     104    1335 
   Size:        104    1231
 
SfoI GGC'GCC

Cuts at:      0     252    1335 
   Size:        252    1083
 
SmlI C'TyrA_G

Cuts at:      0     186     707     930     958    1335 
   Size:        186     521     223      28     377

  Fragments arranged by size: 

                 521     377     223     186      28
 
StyI C'CwwG_G

Cuts at:      0    1152    1335 
   Size:       1152     183
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     214     393     468     820     921    1048    1335 
   Size:        214     179      75     352     101     127     287

  Fragments arranged by size: 

                 352     287     214     179     127     101      75
 
XhoI C'TCGA_G

Cuts at:      0     186     707     930    1335 
   Size:        186     521     223     405

  Fragments arranged by size: 

                 521     405     223     186
 
XmnI GAAnn'nnTTC

Cuts at:      0     208    1335 
   Size:        208    1127
 

 Enzymes that do cut:

     AfeI     ApaI    ApaLI     AvaI     BanI    BanII     BbeI    BbvCI
     BclI     BglI Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrFI   BssHII   BstEII    BstXI     BtgI
     BtsI     DrdI     EaeI     EagI     EciI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV    PflMI   PspOMI    PspXI    SacII     SfoI
     SmlI     StyI    TaqII     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BsaWI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI
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