Restriction map for pF1KB7743
 (Linear) (Six-base) MAPSORT of: KIBB7743.seq  Check: 9116  from: 1  to: 1311



KIBB7743.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:      0     452    1311 
   Size:        452     859
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499    1311 
   Size:        499     812
 
AfeI AGC'GCT

Cuts at:      0     412    1311 
   Size:        412     899
 
AflII C'TTAA_G

Cuts at:      0     856    1311 
   Size:        856     455
 
AflIII A'CryG_T

Cuts at:      0     739    1311 
   Size:        739     572
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1119    1311 
   Size:       1119     192
 
AleI CACnn'nnGTG

Cuts at:      0     586     988    1311 
   Size:        586     402     323

  Fragments arranged by size: 

                 586     402     323
 
AlwNI CAG_nnn'CTG

Cuts at:      0     300     570     657     726     769    1311 
   Size:        300     270      87      69      43     542

  Fragments arranged by size: 

                 542     300     270      87      69      43
 
ApaI G_GGCC'C

Cuts at:      0      18    1311 
   Size:         18    1293
 
AvaI C'yCGr_G

Cuts at:      0      58     323     363    1243    1311 
   Size:         58     265      40     880      68

  Fragments arranged by size: 

                 880     265      68      58      40
 
BanI G'GyrC_C

Cuts at:      0     156     210    1311 
   Size:        156      54    1101

  Fragments arranged by size: 

                1101     156      54
 
BanII G_rGCy'C

Cuts at:      0      18    1101    1311 
   Size:         18    1083     210

  Fragments arranged by size: 

                1083     210      18
 
BbeI G_GCGC'C

Cuts at:      0     160     214    1311 
   Size:        160      54    1097

  Fragments arranged by size: 

                1097     160      54
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     934    1311 
   Size:        934     377
 
BclI T'GATC_A

Cuts at:      0     791    1311 
   Size:        791     520
 
BglI GCCn_nnn'nGGC

Cuts at:      0     136    1311 
   Size:        136    1175
 
BglII A'GATC_T

Cuts at:      0     848    1311 
   Size:        848     463
 
BlpI GC'TnA_GC

Cuts at:      0     427    1311 
   Size:        427     884
 
Bme1580I G_kGCm'C

Cuts at:      0      18     895    1311 
   Size:         18     877     416

  Fragments arranged by size: 

                 877     416      18
 
BmgBI CAC'GTC

Cuts at:      0     740    1311 
   Size:        740     571
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     221     471    1311 
   Size:        221     250     840

  Fragments arranged by size: 

                 840     250     221
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1188    1311 
   Size:       1188     123
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     439    1078    1171    1311 
   Size:         67     372     639      93     140

  Fragments arranged by size: 

                 639     372     140      93      67
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     628    1311 
   Size:        541      87     683

  Fragments arranged by size: 

                 683     541      87
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     379     802     895    1259    1311 
   Size:        379     423      93     364      52

  Fragments arranged by size: 

                 423     379     364      93      52
 
BsaAI yAC'GTr

Cuts at:      0     735    1311 
   Size:        735     576
 
BsaBI GATnn'nnATC

Cuts at:      0     100     673    1311 
   Size:        100     573     638

  Fragments arranged by size: 

                 638     573     100
 
BsaHI Gr'CG_yC

Cuts at:      0     122     157     211     593    1311 
   Size:        122      35      54     382     718

  Fragments arranged by size: 

                 718     382     122      54      35
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      78     913     970     973    1171    1204    1261    1311 
   Size:         78     835      57       3     198      33      57      50

  Fragments arranged by size: 

                 835     198      78      57      57      50      33       3
            
 
BseYI C'CCAG_C

Cuts at:      0     570     812    1036    1311 
   Size:        570     242     224     275

  Fragments arranged by size: 

                 570     275     242     224
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505    1311 
   Size:        505     806
 
BsiEI CG_ry'CG

Cuts at:      0     252     878    1139    1311 
   Size:        252     626     261     172

  Fragments arranged by size: 

                 626     261     252     172
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     895    1101    1311 
   Size:         18     877     206     210

  Fragments arranged by size: 

                 877     210     206      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     194    1311 
   Size:        194    1117
 
BsrDI GCAATG_nn'

Cuts at:      0     536     758    1311 
   Size:        536     222     553

  Fragments arranged by size: 

                 553     536     222
 
BsrFI r'CCGG_y

Cuts at:      0      39      64     135     145     166     258    1311 
   Size:         39      25      71      10      21      92    1053

  Fragments arranged by size: 

                1053      92      71      39      25      21      10
 
BssHII G'CGCG_C

Cuts at:      0      35      68    1311 
   Size:         35      33    1243

  Fragments arranged by size: 

                1243      35      33
 
BssSI C'ACGA_G

Cuts at:      0    1216    1311 
   Size:       1216      95
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     769    1311 
   Size:        769     542
 
BstYI r'GATC_y

Cuts at:      0     848    1032    1311 
   Size:        848     184     279

  Fragments arranged by size: 

                 848     279     184
 
Bsu36I CC'TnA_GG

Cuts at:      0     896    1187    1311 
   Size:        896     291     124

  Fragments arranged by size: 

                 896     291     124
 
BtgI C'CryG_G

Cuts at:      0     152     235     824    1103    1311 
   Size:        152      83     589     279     208

  Fragments arranged by size: 

                 589     279     208     152      83
 
BtsI GCAGTG_nn'

Cuts at:      0     318    1311 
   Size:        318     993
 
ClaI AT'CG_AT

Cuts at:      0     878    1311 
   Size:        878     433
 
DraIII CAC_nnn'GTG

Cuts at:      0     229    1311 
   Size:        229    1082
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     448    1311 
   Size:        448     863
 
EaeI y'GGCC_r

Cuts at:      0       8     249     302    1136    1311 
   Size:          8     241      53     834     175

  Fragments arranged by size: 

                 834     241     175      53       8
 
EagI C'GGCC_G

Cuts at:      0     249    1136    1311 
   Size:        249     887     175

  Fragments arranged by size: 

                 887     249     175
 
EarI CTCTTCn'nnn_

Cuts at:      0     199     837    1276    1311 
   Size:        199     638     439      35

  Fragments arranged by size: 

                 638     439     199      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      99     614    1311 
   Size:         99     515     697

  Fragments arranged by size: 

                 697     515      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     439     499    1078    1171    1311 
   Size:         67     372      60     579      93     140

  Fragments arranged by size: 

                 579     372     140      93      67      60
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1186    1246    1311 
   Size:       1186      60      65

  Fragments arranged by size: 

                1186      65      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14      15      51     624     747    1221    1290    1311 
   Size:         14       1      36     573     123     474      69      21

  Fragments arranged by size: 

                 573     474     123      69      36      21      14       1
            
 
HaeII r_GCGC'y

Cuts at:      0      46     131     160     214     414     726    1311 
   Size:         46      85      29      54     200     312     585

  Fragments arranged by size: 

                 585     312     200      85      54      46      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1105    1137    1311 
   Size:       1105      32     174

  Fragments arranged by size: 

                1105     174      32
 
HincII GTy'rAC

Cuts at:      0     453     617    1311 
   Size:        453     164     694

  Fragments arranged by size: 

                 694     453     164
 
KasI G'GCGC_C

Cuts at:      0     156     210    1311 
   Size:        156      54    1101

  Fragments arranged by size: 

                1101     156      54
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     654    1311 
   Size:        654     657
 
MslI CAynn'nnrTG

Cuts at:      0     586     988    1311 
   Size:        586     402     323

  Fragments arranged by size: 

                 586     402     323
 
MspA1I CmG'CkG

Cuts at:      0     154     237     636     657     690    1071    1128    1311 
   Size:        154      83     399      21      33     381      57     183

  Fragments arranged by size: 

                 399     381     183     154      83      57      33      21
            
 
NaeI GCC'GGC

Cuts at:      0      41      66     137     168    1311 
   Size:         41      25      71      31    1143

  Fragments arranged by size: 

                1143      71      41      31      25
 
NarI GG'CG_CC

Cuts at:      0     157     211    1311 
   Size:        157      54    1100

  Fragments arranged by size: 

                1100     157      54
 
NgoMIV G'CCGG_C

Cuts at:      0      39      64     135     166    1311 
   Size:         39      25      71      31    1145

  Fragments arranged by size: 

                1145      71      39      31      25
 
NspI r_CATG'y

Cuts at:      0    1159    1311 
   Size:       1159     152
 
PasI CC'CwG_GG

Cuts at:      0     922    1311 
   Size:        922     389
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     570     726     825    1311 
   Size:        570     156      99     486

  Fragments arranged by size: 

                 570     486     156      99
 
PfoI T'CCnGG_A

Cuts at:      0     342     416    1078    1148    1311 
   Size:        342      74     662      70     163

  Fragments arranged by size: 

                 662     342     163      74      70
 
PpuMI rG'GwC_Cy

Cuts at:      0     624     747    1290    1311 
   Size:        624     123     543      21

  Fragments arranged by size: 

                 624     543     123      21
 
PspOMI G'GGCC_C

Cuts at:      0      14    1311 
   Size:         14    1297
 
PspXI vC'TCGA_Gb

Cuts at:      0    1243    1311 
   Size:       1243      68
 
PstI C_TGCA'G

Cuts at:      0     641     774    1311 
   Size:        641     133     537

  Fragments arranged by size: 

                 641     537     133
 
PvuI CG_AT'CG

Cuts at:      0     878    1311 
   Size:        878     433
 
PvuII CAG'CTG

Cuts at:      0     636     657     690    1311 
   Size:        636      21      33     621

  Fragments arranged by size: 

                 636     621      33      21
 
SacII CC_GC'GG

Cuts at:      0     155     238    1311 
   Size:        155      83    1073

  Fragments arranged by size: 

                1073     155      83
 
SalI G'TCGA_C

Cuts at:      0     451    1311 
   Size:        451     860
 
SapI GCTCTTCn'nnn_

Cuts at:      0     199    1311 
   Size:        199    1112
 
SfcI C'TryA_G

Cuts at:      0     637     770    1311 
   Size:        637     133     541

  Fragments arranged by size: 

                 637     541     133
 
SfoI GGC'GCC

Cuts at:      0     158     212    1311 
   Size:        158      54    1099

  Fragments arranged by size: 

                1099     158      54
 
SgrAI Cr'CCGG_yG

Cuts at:      0      39     258    1311 
   Size:         39     219    1053

  Fragments arranged by size: 

                1053     219      39
 
SmaI CCC'GGG

Cuts at:      0     365    1311 
   Size:        365     946
 
SmlI C'TyrA_G

Cuts at:      0     520     607     856    1243    1311 
   Size:        520      87     249     387      68

  Fragments arranged by size: 

                 520     387     249      87      68
 
StyI C'CwwG_G

Cuts at:      0       5    1311 
   Size:          5    1306
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     240    1311 
   Size:        240    1071
 
TatI w'GTAC_w

Cuts at:      0     679    1311 
   Size:        679     632
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     338     370    1017    1049    1311 
   Size:        338      32     647      32     262

  Fragments arranged by size: 

                 647     338     262      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     445    1311 
   Size:        445     866
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     414    1311 
   Size:        414     897
 
XhoI C'TCGA_G

Cuts at:      0    1243    1311 
   Size:       1243      68
 
XmaI C'CCGG_G

Cuts at:      0     363    1311 
   Size:        363     948
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII   AflIII     AhdI     AleI    AlwNI
     ApaI     AvaI     BanI    BanII     BbeI     BbsI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI    BsiEI
 Bsp1286I    BspMI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstYI
   Bsu36I     BtgI     BtsI     ClaI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII
     KasI     MmeI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PasI    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI     PvuI
    PvuII    SacII     SalI     SapI     SfcI     SfoI    SgrAI     SmaI
     SmlI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AgeI     AlfI     AloI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI    BfrBI     BmtI   Bpu10I    BsaWI    BsaXI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI    CspCI     DraI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
     PsrI    RsrII     SacI    SanDI     SbfI     ScaI    SexAI     SfiI
     SgfI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     XbaI     XmnI     ZraI
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