Restriction map for pF1KB7041
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AhdI        Alw44I      AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        BamHI       BbrPI       
BciVI       BclI        BfrI        BglII       BsaBI       
BsiWI       BsmI        Bsp1407I    BspDI       BspLU11I    
BsrDI       BsrGI       Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
Eam1105I    EarI        Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     MamI        
MfeI        MluI        MseI        MunI        NdeI        
NheI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tth111I     Van91I      XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         1297  GTCTAC               GT^[AC][GT]AC
AccIII       1508  TCCGGA               T^CCGGA
AgeI          578  ACCGGT               A^CCGGT
AlwI         1560  GATCC                GGATC(4/5)
AlwNI         555  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvrII        1629  CCTAGG               C^CTAGG
BalI         1049  TGGCCA               TGG^CCA
BbvCI         552  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1520  CGACCCAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1630  CTAG                 C^TAG
BlnI         1629  CCTAGG               C^CTAGG
BlpI          432  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      432  GCTGAGC              GC^T[ACGT]AGC
BseAI        1508  TCCGGA               T^CCGGA
BsmBI        1243  CGTCTC               CGTCTC(1/5)
BspEI        1508  TCCGGA               T^CCGGA
BspHI         620  TCATGA               T^CATGA
BssSI        1642  CACGAG               CACGAG(-5/-1)
BstAPI        954  GCACGGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        337  GGTAACC              G^GT[ACGT]ACC
BstPI         337  GGTAACC              G^GT[ACGT]ACC
BstYI        1559  AGATCC               [GA]^GATC[CT]
CelII         432  GCTGAGC              GC^T[ACGT]AGC
DraIII       1642  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        1332  CCTGCGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       337  GGTAACC              G^GT[ACGT]ACC
HinfI        1044  GACTC                G^A[ACGT]TC
KspI          203  CCGCGG               CCGC^GG
MaeI         1630  CTAG                 C^TAG
MflI         1559  AGATCC               [GA]^GATC[CT]
MluNI        1049  TGGCCA               TGG^CCA
MroI         1508  TCCGGA               T^CCGGA
MscI         1049  TGGCCA               TGG^CCA
NaeI         1271  GCCGGC               GCC^GGC
NgoMIV       1271  GCCGGC               G^CCGGC
NotI          637  GCGGCCGC             GC^GGCCGC
NspI          959  GCATGC               [GA]CATG^[CT]
PaeR7I        862  CTCGAG               C^TCGAG
PinAI         578  ACCGGT               A^CCGGT
PleI         1044  GACTC                GAGTC(4/5)
PpuMI          36  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          379  CTGCAG               CTGCA^G
RcaI          620  TCATGA               T^CATGA
SacI          198  GAGCTC               GAGCT^C
SacII         203  CCGCGG               CCGC^GG
SgrAI        1270  CGCCGGCG             C[GA]^CCGG[CT]G
SphI          959  GCATGC               GCATG^C
Tsp45I        388  GTCAC                ^GT[GC]AC
Tsp509I      1679  AATT                 ^AATT
XcmI          464  CCATGGAGGTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          862  CTCGAG               C^TCGAG
XhoII        1559  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Aor51HI       728  AGCGCT               AGC^GCT
             1604  AGCGCT              
ApaI          117  GGGCCC               GGGCC^C
              688  GGGCCC              
AvaII          37  GGACC                G^G[AT]CC
              576  GGACC               
AviII         911  TGCGCA               TGC^GCA
              962  TGCGCA              
BglI          643  GCCATCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              943  GCCGACGGGGC         
BpmI           66  CTCCAG               CTGGAG(16/14)
             1542  CTCCAG              
BsaAI         583  TACGTG               [CT]AC^GT[GA]
              832  TACGTG              
BsaI         1512  GAGACC               GGTCTC(1/5)
             1705  GAGACC              
BseRI         771  CTCCTC               GAGGAG(10/8)
              748  GAGGAG              
BsgI          537  CTGCAC               GTGCAG(16/14)
              561  CTGCAC              
BsrBI        1105  CCGCTC               CCGCTC(-3/-3)
             1195  CCGCTC              
BsrFI         578  ACCGGT               [GA]^CCGG[CT]
             1271  GCCGGC              
Cfr10I        578  ACCGGT               [GA]^CCGG[CT]
             1271  GCCGGC              
DpnII          13  GATC                 ^GATC
             1560  GATC                
EagI          254  CGGCCG               C^GGCCG
              638  CGGCCG              
EclXI         254  CGGCCG               C^GGCCG
              638  CGGCCG              
Eco47III      728  AGCGCT               AGC^GCT
             1604  AGCGCT              
Eco52I        254  CGGCCG               C^GGCCG
              638  CGGCCG              
FspI          911  TGCGCA               TGC^GCA
              962  TGCGCA              
MaeIII        338  GTAAC                ^GT[ACGT]AC
              388  GTCAC               
MboI           13  GATC                 ^GATC
             1560  GATC                
NcoI          464  CCATGG               C^CATGG
              797  CCATGG              
NdeII          13  GATC                 ^GATC
             1560  GATC                
Sau3AI         13  GATC                 ^GATC
             1560  GATC                
SfaNI        1437  GATGC                GCATC(5/9)
              914  GCATC               
SfcI          379  CTGCAG               C^T[GA][CT]AG
             1689  CTACAG              
StuI          893  AGGCCT               AGG^CCT
             1670  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaI        BanI        BanII       BbeI        
BbsI        BbvI        BcnI        BmyI        BpuAI       
BsaHI       BsaJI       BsaWI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        KasI        MaeII       MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NlaIII      
NlaIV       PvuII       RsaI        Sau96I      ScrFI       
SfoI        SmlI        StyI        TaqI        TseI        
TspRI       TthHB8I     
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