Restriction map for pF1KB7041
 (Linear) (Six-base) MAPSORT of: KIBB7041.seq  Check: 3451  from: 1  to: 1716



KIBB7041.orf

 With 182 enzymes: SgfI * 

                        February 7, 2012 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     386    1672    1716 
   Size:        386    1286      44

  Fragments arranged by size: 

                1286     386      44
 
AccI GT'mk_AC

Cuts at:      0    1298    1716 
   Size:       1298     418
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1150    1716 
   Size:       1150     566
 
AfeI AGC'GCT

Cuts at:      0     730    1606    1716 
   Size:        730     876     110

  Fragments arranged by size: 

                 876     730     110
 
AgeI A'CCGG_T

Cuts at:      0     578    1716 
   Size:        578    1138
 
AlwNI CAG_nnn'CTG

Cuts at:      0     560    1716 
   Size:        560    1156
 
ApaI G_GGCC'C

Cuts at:      0     121     692    1716 
   Size:        121     571    1024

  Fragments arranged by size: 

                1024     571     121
 
AvaI C'yCGr_G

Cuts at:      0     862    1350    1432    1716 
   Size:        862     488      82     284

  Fragments arranged by size: 

                 862     488     284      82
 
AvrII C'CTAG_G

Cuts at:      0    1629    1716 
   Size:       1629      87
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     592     625    1716 
   Size:        592      33    1091

  Fragments arranged by size: 

                1091     592      33
 
BanI G'GyrC_C

Cuts at:      0     940    1274    1657    1716 
   Size:        940     334     383      59

  Fragments arranged by size: 

                 940     383     334      59
 
BanII G_rGCy'C

Cuts at:      0      67     121     202     692     713    1716 
   Size:         67      54      81     490      21    1003

  Fragments arranged by size: 

                1003     490      81      67      54      21
 
BbeI G_GCGC'C

Cuts at:      0     944    1278    1661    1716 
   Size:        944     334     383      55

  Fragments arranged by size: 

                 944     383     334      55
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     294     759    1059    1140    1716 
   Size:        294     465     300      81     576

  Fragments arranged by size: 

                 576     465     300     294      81
 
BbvCI CC'TCA_GC

Cuts at:      0     553    1716 
   Size:        553    1163
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1509    1543    1716 
   Size:       1509      34     173

  Fragments arranged by size: 

                1509     173      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     649     949    1716 
   Size:        649     300     767

  Fragments arranged by size: 

                 767     649     300
 
BlpI GC'TnA_GC

Cuts at:      0     433    1716 
   Size:        433    1283
 
Bme1580I G_kGCm'C

Cuts at:      0     121     692     719    1716 
   Size:        121     571      27     997

  Fragments arranged by size: 

                 997     571     121      27
 
BmgBI CAC'GTC

Cuts at:      0     240     528    1716 
   Size:        240     288    1188

  Fragments arranged by size: 

                1188     288     240
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51    1527    1716 
   Size:         51    1476     189

  Fragments arranged by size: 

                1476     189      51
 
Bpu10I CC'TnA_GC

Cuts at:      0      19     553    1716 
   Size:         19     534    1163

  Fragments arranged by size: 

                1163     534      19
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     589     997    1716 
   Size:        589     408     719

  Fragments arranged by size: 

                 719     589     408
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1506    1699    1716 
   Size:       1506     193      17

  Fragments arranged by size: 

                1506     193      17
 
BsaAI yAC'GTr

Cuts at:      0     585     834    1716 
   Size:        585     249     882

  Fragments arranged by size: 

                 882     585     249
 
BsaHI Gr'CG_yC

Cuts at:      0     299     872     941    1145    1275    1634    1658    1716 
   Size:        299     573      69     204     130     359      24      58

  Fragments arranged by size: 

                 573     359     299     204     130      69      58      24
            
 
BsaWI w'CCGG_w

Cuts at:      0     194     578    1446    1508    1716 
   Size:        194     384     868      62     208

  Fragments arranged by size: 

                 868     384     208     194      62
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     184     214    1609    1639    1716 
   Size:        184      30    1395      30      77

  Fragments arranged by size: 

                1395     184      77      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     762     763    1716 
   Size:        762       1     953

  Fragments arranged by size: 

                 953     762       1
 
BseYI C'CCAG_C

Cuts at:      0     612     696    1471    1497    1523    1601    1716 
   Size:        612      84     775      26      26      78     115

  Fragments arranged by size: 

                 775     612     115      84      78      26      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     522     546    1716 
   Size:        522      24    1170

  Fragments arranged by size: 

                1170     522      24
 
BsiEI CG_ry'CG

Cuts at:      0     257     641    1451    1716 
   Size:        257     384     810     265

  Fragments arranged by size: 

                 810     384     265     257
 
BsiHKAI G_wGCw'C

Cuts at:      0     202     784    1076    1716 
   Size:        202     582     292     640

  Fragments arranged by size: 

                 640     582     292     202
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1249    1716 
   Size:       1249     467
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     121     202     692     713     719     784    1076 
   Size:         67      54      81     490      21       6      65     292

Cuts at:   1076    1716 
   Size:        640

  Fragments arranged by size: 

                 640     490     292      81      67      65      54      21
                   6
 
BspEI T'CCGG_A

Cuts at:      0    1508    1716 
   Size:       1508     208
 
BspHI T'CATG_A

Cuts at:      0     620    1716 
   Size:        620    1096
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     325     350     386     434     800    1672    1716 
   Size:        325      25      36      48     366     872      44

  Fragments arranged by size: 

                 872     366     325      48      44      36      25
 
BsrBI CCG'CTC

Cuts at:      0    1107    1197    1716 
   Size:       1107      90     519

  Fragments arranged by size: 

                1107     519      90
 
BsrFI r'CCGG_y

Cuts at:      0     578    1271    1716 
   Size:        578     693     445

  Fragments arranged by size: 

                 693     578     445
 
BssHII G'CGCG_C

Cuts at:      0     278     841    1230    1530    1574    1581    1716 
   Size:        278     563     389     300      44       7     135

  Fragments arranged by size: 

                 563     389     300     278     135      44       7
 
BssSI C'ACGA_G

Cuts at:      0    1642    1716 
   Size:       1642      74
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     960    1716 
   Size:        960     756
 
BstEII G'GTnAC_C

Cuts at:      0     337    1716 
   Size:        337    1379
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     573    1047    1059    1716 
   Size:        573     474      12     657

  Fragments arranged by size: 

                 657     573     474      12
 
BstYI r'GATC_y

Cuts at:      0    1559    1716 
   Size:       1559     157
 
BtgI C'CryG_G

Cuts at:      0     203     329     416     464     521     572     797     935 
   Size:        203     126      87      48      57      51     225     138

Cuts at:    935    1716 
   Size:        781

  Fragments arranged by size: 

                 781     225     203     138     126      87      57      51
                  48
 
DraIII CAC_nnn'GTG

Cuts at:      0    1647    1716 
   Size:       1647      69
 
EaeI y'GGCC_r

Cuts at:      0     254     326     413     419     458     492     638     932 
   Size:        254      72      87       6      39      34     146     294

Cuts at:    932    1037    1049    1302    1649    1682    1716 
   Size:        105      12     253     347      33      34

  Fragments arranged by size: 

                 347     294     254     253     146     105      87      72
                  39      34      34      33      12       6
 
EagI C'GGCC_G

Cuts at:      0     254     638    1716 
   Size:        254     384    1078

  Fragments arranged by size: 

                1078     384     254
 
EcoICRI GAG'CTC

Cuts at:      0     200    1716 
   Size:        200    1516
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51    1150    1527    1716 
   Size:         51    1099     377     189

  Fragments arranged by size: 

                1099     377     189      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1336    1716 
   Size:       1336     380
 
EcoO109I rG'GnC_Cy

Cuts at:      0      37     117     688     689    1716 
   Size:         37      80     571       1    1027

  Fragments arranged by size: 

                1027     571      80      37       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1176    1251    1716 
   Size:       1176      75     465

  Fragments arranged by size: 

                1176     465      75
 
FspI TGC'GCA

Cuts at:      0     913     964    1716 
   Size:        913      51     752

  Fragments arranged by size: 

                 913     752      51
 
HaeII r_GCGC'y

Cuts at:      0     440     732     944     971    1278    1381    1478    1608 
   Size:        440     292     212      27     307     103      97     130

Cuts at:   1608    1661    1716 
   Size:         53      55

  Fragments arranged by size: 

                 440     307     292     212     130     103      97      55
                  53      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1428    1460    1609    1641    1716 
   Size:       1428      32     149      32      75

  Fragments arranged by size: 

                1428     149      75      32      32
 
KasI G'GCGC_C

Cuts at:      0     940    1274    1657    1716 
   Size:        940     334     383      59

  Fragments arranged by size: 

                 940     383     334      59
 
MscI TGG'CCA

Cuts at:      0    1051    1716 
   Size:       1051     665
 
MslI CAynn'nnrTG

Cuts at:      0     322     571    1057    1716 
   Size:        322     249     486     659

  Fragments arranged by size: 

                 659     486     322     249
 
MspA1I CmG'CkG

Cuts at:      0     182     205    1128    1184    1328    1383    1527    1716 
   Size:        182      23     923      56     144      55     144     189

  Fragments arranged by size: 

                 923     189     182     144     144      56      55      23
            
 
NaeI GCC'GGC

Cuts at:      0    1273    1716 
   Size:       1273     443
 
NarI GG'CG_CC

Cuts at:      0     941    1275    1658    1716 
   Size:        941     334     383      58

  Fragments arranged by size: 

                 941     383     334      58
 
NcoI C'CATG_G

Cuts at:      0     464     797    1716 
   Size:        464     333     919

  Fragments arranged by size: 

                 919     464     333
 
NgoMIV G'CCGG_C

Cuts at:      0    1271    1716 
   Size:       1271     445
 
NotI GC'GGCC_GC

Cuts at:      0     638    1716 
   Size:        638    1078
 
NspI r_CATG'y

Cuts at:      0     963    1716 
   Size:        963     753
 
PpuMI rG'GwC_Cy

Cuts at:      0      37    1716 
   Size:         37    1679
 
PspOMI G'GGCC_C

Cuts at:      0     117     688    1716 
   Size:        117     571    1028

  Fragments arranged by size: 

                1028     571     117
 
PspXI vC'TCGA_Gb

Cuts at:      0     862    1716 
   Size:        862     854
 
PstI C_TGCA'G

Cuts at:      0     383    1716 
   Size:        383    1333
 
PvuII CAG'CTG

Cuts at:      0    1128    1184    1527    1716 
   Size:       1128      56     343     189

  Fragments arranged by size: 

                1128     343     189      56
 
SacI G_AGCT'C

Cuts at:      0     202    1716 
   Size:        202    1514
 
SacII CC_GC'GG

Cuts at:      0     206    1716 
   Size:        206    1510
 
SanDI GG'GwC_CC

Cuts at:      0      37    1716 
   Size:         37    1679
 
SfcI C'TryA_G

Cuts at:      0     379    1689    1716 
   Size:        379    1310      27

  Fragments arranged by size: 

                1310     379      27
 
SfoI GGC'GCC

Cuts at:      0     942    1276    1659    1716 
   Size:        942     334     383      57

  Fragments arranged by size: 

                 942     383     334      57
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1271    1716 
   Size:       1271     445
 
SmlI C'TyrA_G

Cuts at:      0     604     862    1012    1716 
   Size:        604     258     150     704

  Fragments arranged by size: 

                 704     604     258     150
 
SphI G_CATG'C

Cuts at:      0     963    1716 
   Size:        963     753
 
StuI AGG'CCT

Cuts at:      0     895    1672    1716 
   Size:        895     777      44

  Fragments arranged by size: 

                 895     777      44
 
StyI C'CwwG_G

Cuts at:      0     464     797     971    1629    1716 
   Size:        464     333     174     658      87

  Fragments arranged by size: 

                 658     464     333     174      87
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     640    1051    1485    1716 
   Size:        640     411     434     231

  Fragments arranged by size: 

                 640     434     411     231
 
TatI w'GTAC_w

Cuts at:      0     818    1716 
   Size:        818     898
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     257     289     517     549    1716 
   Size:        257      32     228      32    1167

  Fragments arranged by size: 

                1167     257     228      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     471    1716 
   Size:        471    1245
 
XhoI C'TCGA_G

Cuts at:      0     862    1716 
   Size:        862     854
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AgeI    AlwNI     ApaI     AvaI
    AvrII     BaeI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BglI     BlpI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI   DraIII
     EaeI     EagI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII    Hin4I     KasI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NotI     NspI    PpuMI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII    SanDI     SfcI     SfoI    SgrAI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AhdI     AleI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    BamHI    BciVI
     BclI    BfrBI    BglII     BmrI     BmtI     BplI    BsaBI    BsiWI
     BsmI    BsrDI    BsrGI    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EarI     EciI    EcoRI    EcoRV
     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     NdeI     NheI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI
     PvuI    RsrII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I
     XbaI     XmaI     XmnI     ZraI
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