Restriction map for pF1KE1689
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AflII       AflIII      AgeI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AviII       AvrII       BamHI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HpaI        HphI        KpnI        KspI        MaeI        
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MroI        MseI        MslI        MunI        
NcoI        NdeI        NdeII       NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        Sau3AI      ScaI        
SexAI       SfiI        SfuI        SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
StyI        SwaI        Tru9I       Tsp45I      Tth111I     
XbaI        XcmI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII         158  CGCG                 CG^CG
AcsI          187  AAATTC               [GA]^AATT[CT]
AhdI          298  GACCTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          187  AAATTC               [GA]^AATT[CT]
AspEI         298  GACCTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI         319  CACGTG               CAC^GTG
BlpI           33  GCTGAGC              GC^T[ACGT]AGC
BpmI          364  CTGGAG               CTGGAG(16/14)
Bpu1102I       33  GCTGAGC              GC^T[ACGT]AGC
BsaAI         319  CACGTG               [CT]AC^GT[GA]
BseRI         492  CTCCTC               GAGGAG(10/8)
BstAPI        324  GCAGTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        528  CATCC                GGATG(2/0)
BstUI         158  CGCG                 CG^CG
CelII          33  GCTGAGC              GC^T[ACGT]AGC
Eam1105I      298  GACCTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          348  CTCTTC               CTCTTC(1/4)
FokI          528  CATCC                GGATG(9/13)
Ksp632I       348  CTCTTC               CTCTTC(1/4)
MaeII         320  ACGT                 A^CGT
MvnI          158  CGCG                 CG^CG
NlaIII        237  CATG                 CATG^
PaeR7I         86  CTCGAG               C^TCGAG
PleI          588  GACTC                GAGTC(4/5)
PmaCI         319  CACGTG               CAC^GTG
PmlI          319  CACGTG               CAC^GTG
SacI          257  GAGCTC               GAGCT^C
SapI          347  GCTCTTC              GCTCTTC(1/4)
SbfI          384  CCTGCAGG             CCTGCA^GG
SfaNI         509  GCATC                GCATC(5/9)
SgrAI         467  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI           86  CTCGAG               C^T[CT][GA]AG
Sse8387I      384  CCTGCAGG             CCTGCA^GG
TaqI           87  TCGA                 T^CGA
TfiI          217  GATTC                G^A[AT]TC
Tsp509I       188  AATT                 ^AATT
TthHB8I        87  TCGA                 T^CGA
XhoI           86  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI          471  GGCGCC               G[GA]^CG[CT]C
              483  GGCGCC              
AlwNI         325  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              421  CAGCTCCTG           
AspHI         127  GTGCTC               G[AT]GC[AT]^C
              257  GAGCTC              
BalI           43  TGGCCA               TGG^CCA
              233  TGGCCA              
BanII         257  GAGCTC               G[GA]GC[CT]^C
              367  GAGCCC              
BbeI          471  GGCGCC               GGCGC^C
              483  GGCGCC              
BbsI          357  GAAGAC               GAAGAC(2/6)
              585  GAAGAC              
BbvCI         494  CCTCAGC              CCTCAGC(-5/-2)
              177  GCTGAGG             
BcnI          336  CCGGG                CC^[GC]GG
              459  CCCGG               
BpuAI         357  GAAGAC               GAAGAC(2/6)
              585  GAAGAC              
BsaHI         471  GGCGCC               G[GA]^CG[CT]C
              483  GGCGCC              
BsiHKAI       127  GTGCTC               G[AT]GC[AT]^C
              257  GAGCTC              
BspMI         311  ACCTGC               ACCTGC(4/8)
              340  GCAGGT              
BsrBI         138  CCGCTC               CCGCTC(-3/-3)
              293  GAGCGG              
EaeI           43  TGGCCA               [CT]^GGCC[GA]
              233  TGGCCA              
HaeII         471  GGCGCC               [GA]GCGC^[CT]
              483  GGCGCC              
Hin1I         471  GGCGCC               G[GA]^CG[CT]C
              483  GGCGCC              
HinfI         217  GATTC                G^A[ACGT]TC
              588  GACTC               
KasI          471  GGCGCC               G^GCGCC
              483  GGCGCC              
MluNI          43  TGGCCA               TGG^CCA
              233  TGGCCA              
MscI           43  TGGCCA               TGG^CCA
              233  TGGCCA              
NaeI          413  GCCGGC               GCC^GGC
              468  GCCGGC              
NarI          471  GGCGCC               GG^CGCC
              483  GGCGCC              
NciI          336  CCGGG                CC^[GC]GG
              459  CCCGG               
NgoMIV        413  GCCGGC               G^CCGGC
              468  GCCGGC              
PflMI          70  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              207  CCACAACCTGG         
PstI          385  CTGCAG               CTGCA^G
              418  CTGCAG              
SfoI          471  GGCGCC               GGC^GCC
              483  GGCGCC              
TspRI         251  CACTG                CA[GC]TG[ACGT][ACGT]^
              325  CAGTG               
Van91I         70  CCACCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              207  CCACAACCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        AvaI        AvaII       BanI        
BbvI        BmyI        BsaJI       BsgI        BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstNI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NlaIV       PvuII       
Sau96I      ScrFI       SfcI        TseI        
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