Restriction map for pF1KE1689
 (Linear) (Six-base) MAPSORT of: KIEE1689.seq  Check: 6986  from: 1  to: 597



KIEE1689.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     331     597 
   Size:        331     266
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     597 
   Size:        376     221
 
AhdI GACnn_n'nnGTC

Cuts at:      0     303     597 
   Size:        303     294
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     185     597 
   Size:        185     412
 
AlwNI CAG_nnn'CTG

Cuts at:      0     330     426     597 
   Size:        330      96     171

  Fragments arranged by size: 

                 330     171      96
 
ApoI r'AATT_y

Cuts at:      0     187     597 
   Size:        187     410
 
AvaI C'yCGr_G

Cuts at:      0      86     104     370     597 
   Size:         86      18     266     227

  Fragments arranged by size: 

                 266     227      86      18
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      41      74     597 
   Size:         41      33     523

  Fragments arranged by size: 

                 523      41      33
 
BanI G'GyrC_C

Cuts at:      0     379     471     483     597 
   Size:        379      92      12     114

  Fragments arranged by size: 

                 379     114      92      12
 
BanII G_rGCy'C

Cuts at:      0     261     371     597 
   Size:        261     110     226

  Fragments arranged by size: 

                 261     226     110
 
BbeI G_GCGC'C

Cuts at:      0     475     487     597 
   Size:        475      12     110

  Fragments arranged by size: 

                 475     110      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     364     592     597 
   Size:        364     228       5

  Fragments arranged by size: 

                 364     228       5
 
BbvCI CC'TCA_GC

Cuts at:      0     178     495     597 
   Size:        178     317     102

  Fragments arranged by size: 

                 317     178     102
 
BlpI GC'TnA_GC

Cuts at:      0      34     597 
   Size:         34     563
 
Bme1580I G_kGCm'C

Cuts at:      0     252     382     597 
   Size:        252     130     215

  Fragments arranged by size: 

                 252     215     130
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     262     567     597 
   Size:        262     305      30

  Fragments arranged by size: 

                 305     262      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     597 
   Size:        385     212
 
Bpu10I CC'TnA_GC

Cuts at:      0     178     495     597 
   Size:        178     317     102

  Fragments arranged by size: 

                 317     178     102
 
BsaAI yAC'GTr

Cuts at:      0     321     597 
   Size:        321     276
 
BsaHI Gr'CG_yC

Cuts at:      0     472     484     597 
   Size:        472      12     113

  Fragments arranged by size: 

                 472     113      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     483     597 
   Size:        483     114
 
BseYI C'CCAG_C

Cuts at:      0     192     375     449     597 
   Size:        192     183      74     148

  Fragments arranged by size: 

                 192     183     148      74
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     263     343     526     597 
   Size:        155     108      80     183      71

  Fragments arranged by size: 

                 183     155     108      80      71
 
BsiHKAI G_wGCw'C

Cuts at:      0     131     261     597 
   Size:        131     130     336

  Fragments arranged by size: 

                 336     131     130
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     252     261     371     382     597 
   Size:        131     121       9     110      11     215

  Fragments arranged by size: 

                 215     131     121     110      11       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     320     331     597 
   Size:        320      11     266

  Fragments arranged by size: 

                 320     266      11
 
BsrBI CCG'CTC

Cuts at:      0     140     295     597 
   Size:        140     155     302

  Fragments arranged by size: 

                 302     155     140
 
BsrFI r'CCGG_y

Cuts at:      0     401     413     468     597 
   Size:        401      12      55     129

  Fragments arranged by size: 

                 401     129      55      12
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     330     597 
   Size:        330     267
 
BtsI GCAGTG_nn'

Cuts at:      0     331     597 
   Size:        331     266
 
EaeI y'GGCC_r

Cuts at:      0      43     233     597 
   Size:         43     190     364

  Fragments arranged by size: 

                 364     190      43
 
EarI CTCTTCn'nnn_

Cuts at:      0     354     597 
   Size:        354     243
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     166     597 
   Size:        166     431
 
EcoICRI GAG'CTC

Cuts at:      0     259     597 
   Size:        259     338
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     385     597 
   Size:        376       9     212

  Fragments arranged by size: 

                 376     212       9
 
HaeII r_GCGC'y

Cuts at:      0     475     487     597 
   Size:        475      12     110

  Fragments arranged by size: 

                 475     110      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     126     158     289     321     597 
   Size:        126      32     131      32     276

  Fragments arranged by size: 

                 276     131     126      32      32
 
KasI G'GCGC_C

Cuts at:      0     471     483     597 
   Size:        471      12     114

  Fragments arranged by size: 

                 471     114      12
 
MscI TGG'CCA

Cuts at:      0      45     235     597 
   Size:         45     190     362

  Fragments arranged by size: 

                 362     190      45
 
MspA1I CmG'CkG

Cuts at:      0      55     165     177     196     480     597 
   Size:         55     110      12      19     284     117

  Fragments arranged by size: 

                 284     117     110      55      19      12
 
NaeI GCC'GGC

Cuts at:      0     415     470     597 
   Size:        415      55     127

  Fragments arranged by size: 

                 415     127      55
 
NarI GG'CG_CC

Cuts at:      0     472     484     597 
   Size:        472      12     113

  Fragments arranged by size: 

                 472     113      12
 
NgoMIV G'CCGG_C

Cuts at:      0     413     468     597 
   Size:        413      55     129

  Fragments arranged by size: 

                 413     129      55
 
PasI CC'CwG_GG

Cuts at:      0      64     597 
   Size:         64     533
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      76     213     597 
   Size:         76     137     384

  Fragments arranged by size: 

                 384     137      76
 
PmlI CAC'GTG

Cuts at:      0     321     597 
   Size:        321     276
 
PspXI vC'TCGA_Gb

Cuts at:      0      86     597 
   Size:         86     511
 
PstI C_TGCA'G

Cuts at:      0     389     422     597 
   Size:        389      33     175

  Fragments arranged by size: 

                 389     175      33
 
PvuII CAG'CTG

Cuts at:      0      55     165     177     196     597 
   Size:         55     110      12      19     401

  Fragments arranged by size: 

                 401     110      55      19      12
 
SacI G_AGCT'C

Cuts at:      0     261     597 
   Size:        261     336
 
SapI GCTCTTCn'nnn_

Cuts at:      0     354     597 
   Size:        354     243
 
SbfI CC_TGCA'GG

Cuts at:      0     389     597 
   Size:        389     208
 
SfcI C'TryA_G

Cuts at:      0     262     385     418     597 
   Size:        262     123      33     179

  Fragments arranged by size: 

                 262     179     123      33
 
SfoI GGC'GCC

Cuts at:      0     473     485     597 
   Size:        473      12     112

  Fragments arranged by size: 

                 473     112      12
 
SgrAI Cr'CCGG_yG

Cuts at:      0     468     597 
   Size:        468     129
 
SmlI C'TyrA_G

Cuts at:      0      86     597 
   Size:         86     511
 
XhoI C'TCGA_G

Cuts at:      0      86     597 
   Size:         86     511
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AlfI    AlwNI     ApoI     AvaI     BaeI
     BanI    BanII     BbeI     BbsI    BbvCI     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BsaAI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI     BtsI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    HaeII    Hin4I     KasI     MscI   MspA1I
     NaeI     NarI   NgoMIV     PasI    PflMI     PmlI    PspXI     PstI
    PvuII     SacI     SapI     SbfI     SfcI     SfoI    SgrAI     SmlI
     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MslI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     ScaI    SexAI     SfiI
     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI     ZraI
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