Restriction map for pF1KE2515
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        AlwNI       AocI        Aor51HI     
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BamHI       BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BseAI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HgaI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII         280  GACGTC               GACGT^C
AccII          18  CGCG                 CG^CG
AcsI           36  GAATTT               [GA]^AATT[CT]
Alw44I        841  GTGCAC               G^TGCAC
ApaI          329  GGGCCC               GGGCC^C
ApaLI         841  GTGCAC               G^TGCAC
ApoI           36  GAATTT               [GA]^AATT[CT]
AviII         212  TGCGCA               TGC^GCA
BbeI          555  GGCGCC               GGCGC^C
BbrPI         409  CACGTG               CAC^GTG
BbvCI         130  GCTGAGG              CCTCAGC(-5/-2)
BcgI          335  GCAAGGTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          706  CTAG                 C^TAG
BglII         989  AGATCT               A^GATCT
BpmI           70  CTCCAG               CTGGAG(16/14)
BsaWI        1103  ACCGGA               [AT]^CCGG[AT]
BspLU11I      668  ACATGT               A^CATGT
BssSI         187  CACGAG               CACGAG(-5/-1)
BstAPI        299  GCATCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          18  CGCG                 CG^CG
DdeI          131  CTGAG                C^T[ACGT]AG
DraIII        409  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
FauI          332  CCCGC                CCCGC(4/6)
FspI          212  TGCGCA               TGC^GCA
HaeII         555  GGCGCC               [GA]GCGC^[CT]
KasI          555  GGCGCC               G^GCGCC
MaeI          706  CTAG                 C^TAG
MseI          695  TTAA                 T^TAA
MslI         1002  CATCGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           18  CGCG                 CG^CG
NarI          555  GGCGCC               GG^CGCC
PmaCI         409  CACGTG               CAC^GTG
PmlI          409  CACGTG               CAC^GTG
PpuMI        1034  GGGACCT              [GA]G^G[AT]CC[CT]
SacI          448  GAGCTC               GAGCT^C
SfoI          555  GGCGCC               GGC^GCC
SmaI          539  CCCGGG               CCC^GGG
SphI         1007  GCATGC               GCATG^C
Tru9I         695  TTAA                 T^TAA
Tsp509I        37  AATT                 ^AATT
XmaCI         539  CCCGGG               C^CCGGG
XmaI          539  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI          280  GACGTC               G[GA]^CG[CT]C
              555  GGCGCC              
AfaI          621  GTAC                 GT^AC
              924  GTAC                
AflIII        653  ACGTGT               A^C[GA][CT]GT
              668  ACATGT              
AlwI          636  GATCC                GGATC(4/5)
              347  GGATC               
BalI           99  TGGCCA               TGG^CCA
              689  TGGCCA              
BbsI          787  GAAGAC               GAAGAC(2/6)
              975  GAAGAC              
BglI           46  GCCACCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              847  GCCCTGGTGGC         
BpuAI         787  GAAGAC               GAAGAC(2/6)
              975  GAAGAC              
BsaAI         409  CACGTG               [CT]AC^GT[GA]
              838  TACGTG              
BsaHI         280  GACGTC               G[GA]^CG[CT]C
              555  GGCGCC              
BstEII        414  GGTGACC              G^GT[ACGT]ACC
              735  GGTCACC             
BstPI         414  GGTGACC              G^GT[ACGT]ACC
              735  GGTCACC             
BstXI         725  CCAAACAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1049  CCATCGACCTGG        
BstYI         635  AGATCC               [GA]^GATC[CT]
              989  AGATCT              
DraII         328  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1034  GGGACCT             
EarI          256  CTCTTC               CTCTTC(1/4)
              502  CTCTTC              
EcoNI         255  CCTCTTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              750  CCTCATAAAGG         
EcoO109I      328  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1034  GGGACCT             
EcoO65I       414  GGTGACC              G^GT[ACGT]ACC
              735  GGTCACC             
EcoT14I        50  CCTTGG               C^C[AT][AT]GG
              320  CCAAGG              
Hin1I         280  GACGTC               G[GA]^CG[CT]C
              555  GGCGCC              
Ksp632I       256  CTCTTC               CTCTTC(1/4)
              502  CTCTTC              
MflI          635  AGATCC               [GA]^GATC[CT]
              989  AGATCT              
MluNI          99  TGGCCA               TGG^CCA
              689  TGGCCA              
MscI           99  TGGCCA               TGG^CCA
              689  TGGCCA              
MspA1I        730  CAGCTG               C[AC]G^C[GT]G
             1072  CAGCTG              
PvuII         730  CAGCTG               CAG^CTG
             1072  CAGCTG              
RsaI          621  GTAC                 GT^AC
              924  GTAC                
StyI           50  CCTTGG               C^C[AT][AT]GG
              320  CCAAGG              
XhoII         635  AGATCC               [GA]^GATC[CT]
              989  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AluI        AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrFI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr10I      Cfr13I      DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TseI        
Tsp45I      TspRI       TthHB8I     
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