Restriction map for
pF1KE2533
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BbeI BbrPI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I CelII CfoI
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 418 GTATAC GT^[AC][GT]AC
AlwNI 911 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 274 GGGCCC GGGCC^C
AspI 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 883 GGATCC G^GATCC
BanI 5 GGCACC G^G[CT][GA]CC
BglII 381 AGATCT A^GATCT
BpmI 447 CTCCAG CTGGAG(16/14)
BsgI 797 CTGCAC GTGCAG(16/14)
BsiEI 288 CGGTCG CG[GA][CT]^CG
BsrDI 1287 CATTGC GCAATG(2/0)
BsrFI 1369 ACCGGC [GA]^CCGG[CT]
Bst1107I 418 GTATAC GTA^TAC
BstXI 461 CCAACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 418 GTATAC GTA^TAC
Cfr10I 1369 ACCGGC [GA]^CCGG[CT]
DraIII 25 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1103 CCTCGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 311 ATGCAT ATGCA^T
NsiI 311 ATGCAT ATGCA^T
NspI 1345 GCATGT [GA]CATG^[CT]
PflFI 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1067 CCATCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 571 CTGCAG CTGCA^G
SacI 847 GAGCTC GAGCT^C
ScaI 803 AGTACT AGT^ACT
TaqI 291 TCGA T^CGA
Tth111I 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 291 TCGA T^CGA
Van91I 1067 CCATCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 177 CCAGCAACTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AspHI 847 GAGCTC G[AT]GC[AT]^C
1402 GAGCAC
BanII 274 GGGCCC G[GA]GC[CT]^C
847 GAGCTC
BbsI 1365 GAAGAC GAAGAC(2/6)
1192 GTCTTC
BbvCI 762 CCTCAGC CCTCAGC(-5/-2)
1140 CCTCAGC
BpuAI 1365 GAAGAC GAAGAC(2/6)
1192 GTCTTC
BsiHKAI 847 GAGCTC G[AT]GC[AT]^C
1402 GAGCAC
BstYI 381 AGATCT [GA]^GATC[CT]
883 GGATCC
FauI 87 CCCGC CCCGC(4/6)
277 CCCGC
MflI 381 AGATCT [GA]^GATC[CT]
883 GGATCC
MslI 864 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1438 CACTCCAATG
PpuMI 592 AGGACCC [GA]G^G[AT]CC[CT]
630 AGGACCC
PshAI 930 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1002 GACCACAGTC
PvuII 195 CAGCTG CAG^CTG
574 CAGCTG
SfcI 192 CTACAG C^T[GA][CT]AG
571 CTGCAG
Tsp509I 234 AATT ^AATT
1554 AATT
XhoII 381 AGATCT [GA]^GATC[CT]
883 GGATCC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
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AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
Tsp45I TspRI