Restriction map for pF1KB5830
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BamHI       BcgI        
BciVI       BfaI        BglI        BglII       BlnI        
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrFI       BsrGI       BssHII      
BstAPI      BstBI       BstEII      BstPI       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HgaI        HindIII     
HpaI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NdeI        NgoMIV      NheI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AcsI         1324  GAATTT               [GA]^AATT[CT]
AcyI          951  GGCGCC               G[GA]^CG[CT]C
AflII        1153  CTTAAG               C^TTAAG
ApoI         1324  GAATTT               [GA]^AATT[CT]
AspI         1246  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          858  TGGCCA               TGG^CCA
BbeI          951  GGCGCC               GGCGC^C
BbrPI         265  CACGTG               CAC^GTG
BbvCI        1227  CCTCAGC              CCTCAGC(-5/-2)
BclI          135  TGATCA               T^GATCA
BfrI         1153  CTTAAG               C^TTAAG
BlpI         1116  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1116  GCTCAGC              GC^T[ACGT]AGC
BsaAI         265  CACGTG               [CT]AC^GT[GA]
BsaHI         951  GGCGCC               G[GA]^CG[CT]C
BsgI          767  GTGCAG               GTGCAG(16/14)
BsmAI         778  GTCTC                GTCTC(1/5)
BsmI          175  GAATGC               GAATGC(1/-1)
BsrBI         748  GAGCGG               CCGCTC(-3/-3)
BsrDI         564  CATTGC               GCAATG(2/0)
BssSI        1063  CACGAG               CACGAG(-5/-1)
Bst1107I      814  GTATAC               GTA^TAC
BstZ17I       814  GTATAC               GTA^TAC
CelII        1116  GCTCAGC              GC^T[ACGT]AGC
EagI         1035  CGGCCG               C^GGCCG
EclXI        1035  CGGCCG               C^GGCCG
Eco52I       1035  CGGCCG               C^GGCCG
FbaI          135  TGATCA               T^GATCA
Hin1I         951  GGCGCC               G[GA]^CG[CT]C
HincII       1300  GTCGAC               GT[CT]^[GA]AC
HindII       1300  GTCGAC               GT[CT]^[GA]AC
KasI          951  GGCGCC               G^GCGCC
MluNI         858  TGGCCA               TGG^CCA
MscI          858  TGGCCA               TGG^CCA
NarI          951  GGCGCC               GG^CGCC
NcoI          356  CCATGG               C^CATGG
NotI         1034  GCGGCCGC             GC^GGCCGC
PflFI        1246  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         265  CACGTG               CAC^GTG
PmlI          265  CACGTG               CAC^GTG
PshAI         240  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          232  CTGCAG               CTGCA^G
PvuII         181  CAGCTG               CAG^CTG
SacI         1078  GAGCTC               GAGCT^C
SalI         1300  GTCGAC               G^TCGAC
SapI           87  GAAGAGC              GCTCTTC(1/4)
ScaI          791  AGTACT               AGT^ACT
SfcI          232  CTGCAG               C^T[GA][CT]AG
SfoI          951  GGCGCC               GGC^GCC
SmaI          725  CCCGGG               CCC^GGG
TfiI          271  GATTC                G^A[AT]TC
Tsp509I      1325  AATT                 ^AATT
Tth111I      1246  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1199  CCAAGGAGGTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         725  CCCGGG               C^CCGGG
XmaI          725  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          814  GTATAC               GT^[AC][GT]AC
             1300  GTCGAC              
AlwI          543  GATCC                GGATC(4/5)
             1098  GATCC               
AocI          955  CCTGAGG              CC^T[ACGT]AGG
             1282  CCTCAGG             
Asp700I       447  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              921  GAAAACCTTC          
AspHI        1078  GAGCTC               G[AT]GC[AT]^C
             1213  GAGCAC              
BanI          623  GGCACC               G^G[CT][GA]CC
              951  GGCGCC              
BbsI          189  GAAGAC               GAAGAC(2/6)
              736  GTCTTC              
BpuAI         189  GAAGAC               GAAGAC(2/6)
              736  GTCTTC              
BsiEI         666  CGACCG               CG[GA][CT]^CG
             1035  CGGCCG              
BsiHKAI      1078  GAGCTC               G[AT]GC[AT]^C
             1213  GAGCAC              
BstXI          36  CCACAAGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              305  CCATCCAGATGG        
BstYI         542  AGATCC               [GA]^GATC[CT]
             1097  AGATCC              
Bsu36I        955  CCTGAGG              CC^T[ACGT]AGG
             1282  CCTCAGG             
Eco81I        955  CCTGAGG              CC^T[ACGT]AGG
             1282  CCTCAGG             
EcoNI         471  CCTTGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              546  CCTGCGGAAGG         
HaeII         951  GGCGCC               [GA]GCGC^[CT]
             1067  AGCGCC              
MflI          542  AGATCC               [GA]^GATC[CT]
             1097  AGATCC              
MseI          154  TTAA                 T^TAA
             1154  TTAA                
PleI          141  GACTC                GAGTC(4/5)
              385  GACTC               
Tru9I         154  TTAA                 T^TAA
             1154  TTAA                
XhoII         542  AGATCC               [GA]^GATC[CT]
             1097  AGATCC              
XmnI          447  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              921  GAAAACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        ApaI        
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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