Restriction map for pF1KB5830
 (Circular) (Six-base) MAPSORT of: pF1KB5830.seq  Check: 9576  from: 1  to: 4556



pF1KB5830  4556 bp

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:27  ..

AccI GT'mk_AC

Cuts at:    923    1409    1594     923 
   Size:        486     185    3885

  Fragments arranged by size: 

                3885     486     185
 
Acc65I G'GTAC_C

Cuts at:   1572    1572 
   Size:       4556
 
AclI AA'CG_TT

Cuts at:   4396    4396 
   Size:       4556
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    214     772    2381    2813    3560    3828     214 
   Size:        558    1609     432     747     268     942

  Fragments arranged by size: 

                1609     942     747     558     432     268
 
AflII C'TTAA_G

Cuts at:   1261    1261 
   Size:       4556
 
AflIII A'CryG_T

Cuts at:   1908    3012    3989    1908 
   Size:       1104     977    2475

  Fragments arranged by size: 

                2475    1104     977
 
AgeI A'CCGG_T

Cuts at:   2944    2944 
   Size:       4556
 
AleI CACnn'nnGTG

Cuts at:    149     149 
   Size:       4556
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1312    1344    1312 
   Size:         32    4524
 
AlwNI CAG_nnn'CTG

Cuts at:   1861    3428    1861 
   Size:       1567    2989
 
ApaI G_GGCC'C

Cuts at:    450     864    1298     450 
   Size:        414     434    3708

  Fragments arranged by size: 

                3708     434     414
 
ApaLI G'TGCA_C

Cuts at:   3326    3326 
   Size:       4556
 
ApoI r'AATT_y

Cuts at:   1432    1560    3909    4165    1432 
   Size:        128    2349     256    1823

  Fragments arranged by size: 

                2349    1823     256     128
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4556
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4556
 
AvaI C'yCGr_G

Cuts at:    232     833    1231    1576     232 
   Size:        601     398     345    3212

  Fragments arranged by size: 

                3212     601     398     345
 
BamHI G'GATC_C

Cuts at:   1581    1581 
   Size:       4556
 
BanI G'GyrC_C

Cuts at:    731    1059    1572    1986    2234    2269     731 
   Size:        328     513     414     248      35    3018

  Fragments arranged by size: 

                3018     513     414     328     248      35
 
BanII G_rGCy'C

Cuts at:    450     864    1190    1298    1570    2600     450 
   Size:        414     326     108     272    1030    2406

  Fragments arranged by size: 

                2406    1030     414     326     272     108
 
BbeI G_GCGC'C

Cuts at:   1063    1990    2238    1063 
   Size:        927     248    3381

  Fragments arranged by size: 

                3381     927     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    304     837     304 
   Size:        533    4023
 
BbvCI CC'TCA_GC

Cuts at:   1336    1336 
   Size:       4556
 
BciVI GTATCCnnnnn_n'

Cuts at:   2454    3215    4556    2454 
   Size:        761    1341    2454

  Fragments arranged by size: 

                2454    1341     761
 
BclI T'GATC_A

Cuts at:    243    2076     243 
   Size:       1833    2723
 
BglII A'GATC_T

Cuts at:   2071    4108    2071 
   Size:       2037    2519
 
BlpI GC'TnA_GC

Cuts at:   1225    1668    1225 
   Size:        443    4113
 
Bme1580I G_kGCm'C

Cuts at:    450     864    1298    2181    2274    3330     450 
   Size:        414     434     883      93    1056    1676

  Fragments arranged by size: 

                1676    1056     883     434     414      93
 
BmgBI CAC'GTC

Cuts at:    918    1356    1400     918 
   Size:        438      44    4074

  Fragments arranged by size: 

                4074     438      44
 
BmrI ACTGGGnnnn_n'

Cuts at:    482    1437    1515    1768    1849    2184    4328     482 
   Size:        955      78     253      81     335    2144     710

  Fragments arranged by size: 

                2144     955     710     335     253      81      78
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    903    2923     903 
   Size:       2020    2536
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    192     368     703    1093    1513     192 
   Size:        176     335     390     420    3235

  Fragments arranged by size: 

                3235     420     390     335     176
 
Bpu10I CC'TnA_GC

Cuts at:   1336    1336 
   Size:       4556
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    421     916    1732    2611    3103    3401     421 
   Size:        495     816     879     492     298    1576

  Fragments arranged by size: 

                1576     879     816     495     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4260    4260 
   Size:       4556
 
BsaAI yAC'GTr

Cuts at:    375    1909     375 
   Size:       1534    3022
 
BsaBI GATnn'nnATC

Cuts at:      6    2070    4107       6 
   Size:       2064    2037     455

  Fragments arranged by size: 

                2064    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1060    1987    2235    2937    4433    1060 
   Size:        927     248     702    1496    1183

  Fragments arranged by size: 

                1496    1183     927     702     248
 
BsaWI w'CCGG_w

Cuts at:   1745    2266    2944    3218    3365    3776    1745 
   Size:        521     678     274     147     411    2525

  Fragments arranged by size: 

                2525     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    256     286    1312    1342     256 
   Size:         30    1026      30    3470

  Fragments arranged by size: 

                3470    1026      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    412     486     523     868     412 
   Size:         74      37     345    4100

  Fragments arranged by size: 

                4100     345      74      37
 
BseYI C'CCAG_C

Cuts at:   1113    1252    1297    1981    2739    3316    3812    1113 
   Size:        139      45     684     758     577     496    1857

  Fragments arranged by size: 

                1857     758     684     577     496     139      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    896     896 
   Size:       4556
 
BsiEI CG_ry'CG

Cuts at:    104     777    1146    2098    2144    3352     104 
   Size:        673     369     952      46    1208    1308

  Fragments arranged by size: 

                1308    1208     952     673     369      46
 
BsiHKAI G_wGCw'C

Cuts at:   1190    1325    1570    2348    2538    3330    1190 
   Size:        135     245     778     190     792    2416

  Fragments arranged by size: 

                2416     792     778     245     190     135
 
BsmI GAATG_Cn'

Cuts at:    289     289 
   Size:       4556
 
Bsp1286I G_dGCh'C

Cuts at:    450     864    1190    1298    1325    1570    2181    2274    2348 
   Size:        414     326     108      27     245     611      93      74

Cuts at:   2348    2538    2600    3330     450 
   Size:        190      62     730    1676

  Fragments arranged by size: 

                1676     730     611     414     326     245     190     108
                  93      74      62      27
 
BspEI T'CCGG_A

Cuts at:   3776    3776 
   Size:       4556
 
BspHI T'CATG_A

Cuts at:   3732    3732 
   Size:       4556
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1606    1820    2122    2503    1606 
   Size:        214     302     381    3659

  Fragments arranged by size: 

                3659     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     858    2848    2902    4386    4551      50 
   Size:        808    1990      54    1484     165      55

  Fragments arranged by size: 

                1990    1484     808     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    671    2468     671 
   Size:       1797    2759
 
BsrFI r'CCGG_y

Cuts at:   2554    2735    2944    2554 
   Size:        181     209    4166

  Fragments arranged by size: 

                4166     209     181
 
BssSI C'ACGA_G

Cuts at:   1171    2827    3185    1171 
   Size:       1656     358    2542

  Fragments arranged by size: 

                2542    1656     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1933    1933 
   Size:       4556
 
BstBI TT'CG_AA

Cuts at:   2917    2917 
   Size:       4556
 
BstXI CCAn_nnnn'nTGG

Cuts at:    151     420     151 
   Size:        269    4287
 
BstYI r'GATC_y

Cuts at:      1     650    1205    1581    2048    2071    2406    2652    3664 
   Size:        649     555     376     467      23     335     246    1012

Cuts at:   3664    3750    3762    4108       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     649     555     467     449     376     346     335
                 246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    924     924 
   Size:       4556
 
Bsu36I CC'TnA_GG

Cuts at:   1064    1391    1064 
   Size:        327    4229
 
BtgI C'CryG_G

Cuts at:    178     464    1097     178 
   Size:        286     633    3637

  Fragments arranged by size: 

                3637     633     286
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1316    2506    2687    2850    1316 
   Size:       1190     181     163    3022

  Fragments arranged by size: 

                3022    1190     181     163
 
BtsI GCAGTG_nn'

Cuts at:    349     743    1799     349 
   Size:        394    1056    3106

  Fragments arranged by size: 

                3106    1056     394
 
DraI TTT'AAA

Cuts at:   1555    1555 
   Size:       4556
 
DrdI GACnn_nn'nnGTC

Cuts at:   2262    3120    2262 
   Size:        858    3698
 
EaeI y'GGCC_r

Cuts at:    630     966    1089    1143    1535    2141    2315    2706    2733 
   Size:        336     123      54     392     606     174     391      27

Cuts at:   2733    4078     630 
   Size:       1345    1108

  Fragments arranged by size: 

                1345    1108     606     392     391     336     174     123
                  54      27
 
EagI C'GGCC_G

Cuts at:   1143    2141    1143 
   Size:        998    3558
 
EarI CTCTTCn'nnn_

Cuts at:    190    1186    1196    2579    2789     190 
   Size:        996      10    1383     210    1957

  Fragments arranged by size: 

                1957    1383     996     210      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2764    3074    3220    4366    2764 
   Size:        310     146    1146    2954

  Fragments arranged by size: 

                2954    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1188    1568    1188 
   Size:        380    4176
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    192     214     368     703     772    1093    1513    2381    2813 
   Size:         22     154     335      69     321     420     868     432

Cuts at:   2813    3560    3828     192 
   Size:        747     268     920

  Fragments arranged by size: 

                 920     868     747     432     420     335     321     268
                 154      69      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:    583     658     583 
   Size:         75    4481
 
EcoO109I rG'GnC_Cy

Cuts at:    237     365     447     459     714    1043    1269    1294    1695 
   Size:        128      82      12     255     329     226      25     401

Cuts at:   1695     237 
   Size:       3098

  Fragments arranged by size: 

                3098     401     329     255     226     128      82      25
                  12
 
EcoRI G'AATT_C

Cuts at:   1560    1560 
   Size:       4556
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    609     777    2575     609 
   Size:        168    1798    2590

  Fragments arranged by size: 

                2590    1798     168
 
FspI TGC'GCA

Cuts at:   2337    2337 
   Size:       4556
 
HaeII r_GCGC'y

Cuts at:   1063    1179    1990    2238    3260    4239    1063 
   Size:        116     811     248    1022     979    1380

  Fragments arranged by size: 

                1380    1022     979     811     248     116
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1225    1257    2398    2428    2430    2460    1225 
   Size:         32    1141      30       2      30    3321

  Fragments arranged by size: 

                3321    1141      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1410    1595    1410 
   Size:        185    4371
 
KasI G'GCGC_C

Cuts at:   1059    1986    2234    1059 
   Size:        927     248    3381

  Fragments arranged by size: 

                3381     927     248
 
KpnI G_GTAC'C

Cuts at:   1576    1576 
   Size:       4556
 
MluI A'CGCG_T

Cuts at:   3989    3989 
   Size:       4556
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3227    3411    3227 
   Size:        184    4372
 
MscI TGG'CCA

Cuts at:    968    2317     968 
   Size:       1349    3207
 
MslI CAynn'nnrTG

Cuts at:    149     418     610    1195    2545    2672     149 
   Size:        269     192     585    1350     127    2033

  Fragments arranged by size: 

                2033    1350     585     269     192     127
 
MspA1I CmG'CkG

Cuts at:    291     444    1506    1667    1981    2341    3354    3599     291 
   Size:        153    1062     161     314     360    1013     245    1248

  Fragments arranged by size: 

                1248    1062    1013     360     314     245     161     153
            
 
NaeI GCC'GGC

Cuts at:   2737    2737 
   Size:       4556
 
NarI GG'CG_CC

Cuts at:   1060    1987    2235    1060 
   Size:        927     248    3381

  Fragments arranged by size: 

                3381     927     248
 
NcoI C'CATG_G

Cuts at:    464     464 
   Size:       4556
 
NgoMIV G'CCGG_C

Cuts at:   2735    2735 
   Size:       4556
 
NotI GC'GGCC_GC

Cuts at:   1143    1143 
   Size:       4556
 
NspI r_CATG'y

Cuts at:   1609    2109    3016    1609 
   Size:        500     907    3149

  Fragments arranged by size: 

                3149     907     500
 
PciI A'CATG_T

Cuts at:   3012    3012 
   Size:       4556
 
PmeI GTTT'AAAC

Cuts at:   1555    1555 
   Size:       4556
 
PmlI CAC'GTG

Cuts at:    375     375 
   Size:       4556
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1312    1344    3695    3727    1312 
   Size:         32    2351      32    2141

  Fragments arranged by size: 

                2351    2141      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    365     459     714    1043    1269     365 
   Size:         94     255     329     226    3652

  Fragments arranged by size: 

                3652     329     255     226      94
 
PshAI GACnn'nnGTC

Cuts at:    352     352 
   Size:       4556
 
PspOMI G'GGCC_C

Cuts at:    446     860    1294     446 
   Size:        414     434    3708

  Fragments arranged by size: 

                3708     434     414
 
PstI C_TGCA'G

Cuts at:    344    1603    2288     344 
   Size:       1259     685    2612

  Fragments arranged by size: 

                2612    1259     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4556
 
PvuII CAG'CTG

Cuts at:    291    1981    2341     291 
   Size:       1690     360    2506

  Fragments arranged by size: 

                2506    1690     360
 
RsrII CG'GwC_CG

Cuts at:   2751    2751 
   Size:       4556
 
SacI G_AGCT'C

Cuts at:   1190    1570    1190 
   Size:        380    4176
 
SalI G'TCGA_C

Cuts at:   1408    1593    1408 
   Size:        185    4371
 
SanDI GG'GwC_CC

Cuts at:   1043    1043 
   Size:       4556
 
SapI GCTCTTCn'nnn_

Cuts at:    190    2579    2789     190 
   Size:       2389     210    1957

  Fragments arranged by size: 

                2389    1957     210
 
SbfI CC_TGCA'GG

Cuts at:   1603    1603 
   Size:       4556
 
ScaI AGT'ACT

Cuts at:    901    4073     901 
   Size:       3172    1384
 
SfcI C'TryA_G

Cuts at:     33     340    1599    2284    3277    3468      33 
   Size:        307    1259     685     993     191    1121

  Fragments arranged by size: 

                1259    1121     993     685     307     191
 
SfoI GGC'GCC

Cuts at:   1061    1988    2236    1061 
   Size:        927     248    3381

  Fragments arranged by size: 

                3381     927     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4556
 
SmaI CCC'GGG

Cuts at:    835    1578     835 
   Size:        743    3813
 
SmlI C'TyrA_G

Cuts at:    436     895    1261    1711    2626    3118    3380     436 
   Size:        459     366     450     915     492     262    1612

  Fragments arranged by size: 

                1612     915     492     459     450     366     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4556
 
SphI G_CATG'C

Cuts at:   1609    2109    1609 
   Size:        500    4056
 
StyI C'CwwG_G

Cuts at:    464     579     677    1307    1690    2044     464 
   Size:        115      98     630     383     354    2976

  Fragments arranged by size: 

                2976     630     383     354     115      98
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    316    1105    2139    2272    2731    2940     316 
   Size:        789    1034     133     459     209    1932

  Fragments arranged by size: 

                1932    1034     789     459     209     133
 
TatI w'GTAC_w

Cuts at:    899    4071     899 
   Size:       3172    1384
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    170     202     460     492    4073    4105     170 
   Size:         32     258      32    3581      32     621

  Fragments arranged by size: 

                3581     621     258      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1357    2353    1357 
   Size:        996    3560
 
XbaI T'CTAG_A

Cuts at:   1587    1587 
   Size:       4556
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1314    2051    1314 
   Size:        737    3819
 
XmaI C'CCGG_G

Cuts at:    833    1576     833 
   Size:        743    3813
 
XmnI GAAnn'nnTTC

Cuts at:    559    1033     559 
   Size:        474    4082
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NspI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AlfI     AscI    AvrII     BaeI
     BcgI    BfrBI     BglI     BmtI    BsiWI    BsmBI    BsrGI   BssHII
   BstEII     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PsiI    PspXI     PsrI    SacII    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com