Restriction map for pF1KB5830
 (Linear) (Six-base) MAPSORT of: KIBB5830.seq  Check: 7670  from: 1  to: 1443



KIBB5830.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:27  ..

AccI GT'mk_AC

Cuts at:      0     815    1301    1443 
   Size:        815     486     142

  Fragments arranged by size: 

                 815     486     142
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     664    1443 
   Size:        106     558     779

  Fragments arranged by size: 

                 779     558     106
 
AflII C'TTAA_G

Cuts at:      0    1153    1443 
   Size:       1153     290
 
AleI CACnn'nnGTG

Cuts at:      0      41    1443 
   Size:         41    1402
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1204    1236    1443 
   Size:       1204      32     207

  Fragments arranged by size: 

                1204     207      32
 
ApaI G_GGCC'C

Cuts at:      0     342     756    1190    1443 
   Size:        342     414     434     253

  Fragments arranged by size: 

                 434     414     342     253
 
ApoI r'AATT_y

Cuts at:      0    1324    1443 
   Size:       1324     119
 
AvaI C'yCGr_G

Cuts at:      0     124     725    1123    1443 
   Size:        124     601     398     320

  Fragments arranged by size: 

                 601     398     320     124
 
BanI G'GyrC_C

Cuts at:      0     623     951    1443 
   Size:        623     328     492

  Fragments arranged by size: 

                 623     492     328
 
BanII G_rGCy'C

Cuts at:      0     342     756    1082    1190    1443 
   Size:        342     414     326     108     253

  Fragments arranged by size: 

                 414     342     326     253     108
 
BbeI G_GCGC'C

Cuts at:      0     955    1443 
   Size:        955     488
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     196     729    1443 
   Size:        196     533     714

  Fragments arranged by size: 

                 714     533     196
 
BbvCI CC'TCA_GC

Cuts at:      0    1228    1443 
   Size:       1228     215
 
BclI T'GATC_A

Cuts at:      0     135    1443 
   Size:        135    1308
 
BlpI GC'TnA_GC

Cuts at:      0    1117    1443 
   Size:       1117     326
 
Bme1580I G_kGCm'C

Cuts at:      0     342     756    1190    1443 
   Size:        342     414     434     253

  Fragments arranged by size: 

                 434     414     342     253
 
BmgBI CAC'GTC

Cuts at:      0     810    1248    1292    1443 
   Size:        810     438      44     151

  Fragments arranged by size: 

                 810     438     151      44
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     374    1329    1407    1443 
   Size:        374     955      78      36

  Fragments arranged by size: 

                 955     374      78      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     795    1443 
   Size:        795     648
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     260     595     985    1405    1443 
   Size:         84     176     335     390     420      38

  Fragments arranged by size: 

                 420     390     335     176      84      38
 
Bpu10I CC'TnA_GC

Cuts at:      0    1228    1443 
   Size:       1228     215
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     808    1443 
   Size:        313     495     635

  Fragments arranged by size: 

                 635     495     313
 
BsaAI yAC'GTr

Cuts at:      0     267    1443 
   Size:        267    1176
 
BsaHI Gr'CG_yC

Cuts at:      0     952    1443 
   Size:        952     491
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     148     178    1204    1234    1443 
   Size:        148      30    1026      30     209

  Fragments arranged by size: 

                1026     209     148      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     304     378     415     760    1443 
   Size:        304      74      37     345     683

  Fragments arranged by size: 

                 683     345     304      74      37
 
BseYI C'CCAG_C

Cuts at:      0    1005    1144    1189    1443 
   Size:       1005     139      45     254

  Fragments arranged by size: 

                1005     254     139      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     788    1443 
   Size:        788     655
 
BsiEI CG_ry'CG

Cuts at:      0     669    1038    1443 
   Size:        669     369     405

  Fragments arranged by size: 

                 669     405     369
 
BsiHKAI G_wGCw'C

Cuts at:      0    1082    1217    1443 
   Size:       1082     135     226

  Fragments arranged by size: 

                1082     226     135
 
BsmI GAATG_Cn'

Cuts at:      0     181    1443 
   Size:        181    1262
 
Bsp1286I G_dGCh'C

Cuts at:      0     342     756    1082    1190    1217    1443 
   Size:        342     414     326     108      27     226

  Fragments arranged by size: 

                 414     342     326     226     108      27
 
BsrBI CCG'CTC

Cuts at:      0     750    1443 
   Size:        750     693
 
BsrDI GCAATG_nn'

Cuts at:      0     563    1443 
   Size:        563     880
 
BssSI C'ACGA_G

Cuts at:      0    1063    1443 
   Size:       1063     380
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      43     312    1443 
   Size:         43     269    1131

  Fragments arranged by size: 

                1131     269      43
 
BstYI r'GATC_y

Cuts at:      0     542    1097    1443 
   Size:        542     555     346

  Fragments arranged by size: 

                 555     542     346
 
BstZ17I GTA'TAC

Cuts at:      0     816    1443 
   Size:        816     627
 
Bsu36I CC'TnA_GG

Cuts at:      0     956    1283    1443 
   Size:        956     327     160

  Fragments arranged by size: 

                 956     327     160
 
BtgI C'CryG_G

Cuts at:      0      70     356     989    1443 
   Size:         70     286     633     454

  Fragments arranged by size: 

                 633     454     286      70
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1208    1443 
   Size:       1208     235
 
BtsI GCAGTG_nn'

Cuts at:      0     241     635    1443 
   Size:        241     394     808

  Fragments arranged by size: 

                 808     394     241
 
EaeI y'GGCC_r

Cuts at:      0     522     858     981    1035    1427    1443 
   Size:        522     336     123      54     392      16

  Fragments arranged by size: 

                 522     392     336     123      54      16
 
EagI C'GGCC_G

Cuts at:      0    1035    1443 
   Size:       1035     408
 
EarI CTCTTCn'nnn_

Cuts at:      0      82    1078    1088    1443 
   Size:         82     996      10     355

  Fragments arranged by size: 

                 996     355      82      10
 
EcoICRI GAG'CTC

Cuts at:      0    1080    1443 
   Size:       1080     363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     106     260     595     664     985    1405    1443 
   Size:         84      22     154     335      69     321     420      38

  Fragments arranged by size: 

                 420     335     321     154      84      69      38      22
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     475     550    1443 
   Size:        475      75     893

  Fragments arranged by size: 

                 893     475      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129     257     339     351     606     935    1161    1186 
   Size:        129     128      82      12     255     329     226      25

Cuts at:   1186    1443 
   Size:        257

  Fragments arranged by size: 

                 329     257     255     226     129     128      82      25
                  12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     501     669    1443 
   Size:        501     168     774

  Fragments arranged by size: 

                 774     501     168
 
HaeII r_GCGC'y

Cuts at:      0     955    1071    1443 
   Size:        955     116     372

  Fragments arranged by size: 

                 955     372     116
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1117    1149    1443 
   Size:       1117      32     294

  Fragments arranged by size: 

                1117     294      32
 
HincII GTy'rAC

Cuts at:      0    1302    1443 
   Size:       1302     141
 
KasI G'GCGC_C

Cuts at:      0     951    1443 
   Size:        951     492
 
MscI TGG'CCA

Cuts at:      0     860    1443 
   Size:        860     583
 
MslI CAynn'nnrTG

Cuts at:      0      41     310     502    1087    1443 
   Size:         41     269     192     585     356

  Fragments arranged by size: 

                 585     356     269     192      41
 
MspA1I CmG'CkG

Cuts at:      0     183     336    1398    1443 
   Size:        183     153    1062      45

  Fragments arranged by size: 

                1062     183     153      45
 
NarI GG'CG_CC

Cuts at:      0     952    1443 
   Size:        952     491
 
NcoI C'CATG_G

Cuts at:      0     356    1443 
   Size:        356    1087
 
NotI GC'GGCC_GC

Cuts at:      0    1035    1443 
   Size:       1035     408
 
PmlI CAC'GTG

Cuts at:      0     267    1443 
   Size:        267    1176
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1204    1236    1443 
   Size:       1204      32     207

  Fragments arranged by size: 

                1204     207      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     257     351     606     935    1161    1443 
   Size:        257      94     255     329     226     282

  Fragments arranged by size: 

                 329     282     257     255     226      94
 
PshAI GACnn'nnGTC

Cuts at:      0     244    1443 
   Size:        244    1199
 
PspOMI G'GGCC_C

Cuts at:      0     338     752    1186    1443 
   Size:        338     414     434     257

  Fragments arranged by size: 

                 434     414     338     257
 
PstI C_TGCA'G

Cuts at:      0     236    1443 
   Size:        236    1207
 
PvuII CAG'CTG

Cuts at:      0     183    1443 
   Size:        183    1260
 
SacI G_AGCT'C

Cuts at:      0    1082    1443 
   Size:       1082     361
 
SalI G'TCGA_C

Cuts at:      0    1300    1443 
   Size:       1300     143
 
SanDI GG'GwC_CC

Cuts at:      0     935    1443 
   Size:        935     508
 
SapI GCTCTTCn'nnn_

Cuts at:      0      82    1443 
   Size:         82    1361
 
ScaI AGT'ACT

Cuts at:      0     793    1443 
   Size:        793     650
 
SfcI C'TryA_G

Cuts at:      0     232    1443 
   Size:        232    1211
 
SfoI GGC'GCC

Cuts at:      0     953    1443 
   Size:        953     490
 
SmaI CCC'GGG

Cuts at:      0     727    1443 
   Size:        727     716
 
SmlI C'TyrA_G

Cuts at:      0     328     787    1153    1443 
   Size:        328     459     366     290

  Fragments arranged by size: 

                 459     366     328     290
 
StyI C'CwwG_G

Cuts at:      0     356     471     569    1199    1443 
   Size:        356     115      98     630     244

  Fragments arranged by size: 

                 630     356     244     115      98
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     208     997    1443 
   Size:        208     789     446

  Fragments arranged by size: 

                 789     446     208
 
TatI w'GTAC_w

Cuts at:      0     791    1443 
   Size:        791     652
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      62      94     352     384    1443 
   Size:         62      32     258      32    1059

  Fragments arranged by size: 

                1059     258      62      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1249    1443 
   Size:       1249     194
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1206    1443 
   Size:       1206     237
 
XmaI C'CCGG_G

Cuts at:      0     725    1443 
   Size:        725     718
 
XmnI GAAnn'nnTTC

Cuts at:      0     451     925    1443 
   Size:        451     474     518

  Fragments arranged by size: 

                 518     474     451
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AleI     AloI     ApaI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI    BbvCI     BclI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BsrBI    BsrDI    BssSI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I   HincII     KasI     MscI     MslI
   MspA1I     NarI     NcoI     NotI     PmlI     PpiI    PpuMI    PshAI
   PspOMI     PstI    PvuII     SacI     SalI    SanDI     SapI     ScaI
     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AlfI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BcgI    BciVI    BfrBI     BglI    BglII     BmtI     BsaI
    BsaBI    BsaWI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII     ClaI    CspCI     DraI
   DraIII     DrdI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI     NdeI
   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SbfI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XhoI     ZraI
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