Restriction map for
pF1KE2025
##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbrPI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI MaeI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI SacI
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 871 GACGTC GACGT^C
AccIII 1034 TCCGGA T^CCGGA
AcsI 1123 AAATTC [GA]^AATT[CT]
AlwNI 160 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 70 GGGCCC GGGCC^C
ApoI 1123 AAATTC [GA]^AATT[CT]
BalI 1046 TGGCCA TGG^CCA
BbsI 429 GTCTTC GAAGAC(2/6)
BglI 272 GCCGTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 429 GTCTTC GAAGAC(2/6)
BsaAI 496 TACGTG [CT]AC^GT[GA]
BsaBI 1107 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1034 TCCGGA [AT]^CCGG[AT]
BseAI 1034 TCCGGA T^CCGGA
BsiEI 1544 CGACCG CG[GA][CT]^CG
BsmAI 259 GAGAC GTCTC(1/5)
BsmI 1137 GCATTC GAATGC(1/-1)
BspEI 1034 TCCGGA T^CCGGA
BssSI 1719 CACGAG CACGAG(-5/-1)
BstAPI 159 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 695 CGGACCG CG^G[AT]CCG
DrdI 420 GACGGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1738 GTCAAC GT[CT]^[GA]AC
HindII 1738 GTCAAC GT[CT]^[GA]AC
KspI 986 CCGCGG CCGC^GG
MamI 1107 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1046 TGGCCA TGG^CCA
MroI 1034 TCCGGA T^CCGGA
MscI 1046 TGGCCA TGG^CCA
MseI 239 TTAA T^TAA
NaeI 184 GCCGGC GCC^GGC
NgoMIV 184 GCCGGC G^CCGGC
PaeR7I 172 CTCGAG C^TCGAG
PpuMI 1684 GGGTCCT [GA]G^G[AT]CC[CT]
RsrII 695 CGGACCG CG^G[AT]CCG
SacII 986 CCGCGG CCGC^GG
SapI 786 GCTCTTC GCTCTTC(1/4)
ScaI 443 AGTACT AGT^ACT
SexAI 1247 ACCAGGT A^CC[AT]GGT
SfcI 213 CTACAG C^T[GA][CT]AG
SphI 800 GCATGC GCATG^C
TaqI 173 TCGA T^CGA
TfiI 1605 GATTC G^A[AT]TC
Tru9I 239 TTAA T^TAA
Tsp509I 1124 AATT ^AATT
TthHB8I 173 TCGA T^CGA
XhoI 172 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 938 GGTACC G^GTACC
1244 GGTACC
Asp718I 938 GGTACC G^GTACC
1244 GGTACC
BamHI 587 GGATCC G^GATCC
1037 GGATCC
BbeI 100 GGCGCC GGCGC^C
1693 GGCGCC
BbvCI 1512 CCTCAGC CCTCAGC(-5/-2)
664 GCTGAGG
BsgI 267 GTGCAG GTGCAG(16/14)
439 GTGCAG
BspMI 376 ACCTGC ACCTGC(4/8)
747 GCAGGT
BsrBI 397 GAGCGG CCGCTC(-3/-3)
415 GAGCGG
BsrFI 184 GCCGGC [GA]^CCGG[CT]
1375 ACCGGC
BssHII 180 GCGCGC G^CGCGC
519 GCGCGC
Cfr10I 184 GCCGGC [GA]^CCGG[CT]
1375 ACCGGC
EaeI 1046 TGGCCA [CT]^GGCC[GA]
1526 TGGCCG
EarI 787 CTCTTC CTCTTC(1/4)
678 GAAGAG
KasI 100 GGCGCC G^GCGCC
1693 GGCGCC
KpnI 938 GGTACC GGTAC^C
1244 GGTACC
Ksp632I 787 CTCTTC CTCTTC(1/4)
678 GAAGAG
MslI 220 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1770 CATCTCCATG
NarI 100 GGCGCC GG^CGCC
1693 GGCGCC
PleI 193 GACTC GAGTC(4/5)
427 GAGTC
SfoI 100 GGCGCC GGC^GCC
1693 GGCGCC
SmaI 57 CCCGGG CCC^GGG
714 CCCGGG
SmlI 172 CTCGAG C^T[CT][GA]AG
1557 CTTGAG
XcmI 1388 CCAGTAACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1698 CCAGGGCAGTGGTGG
XmaCI 57 CCCGGG C^CCGGG
714 CCCGGG
XmaI 57 CCCGGG C^CCGGG
714 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI Van91I
XhoII