Restriction map for
pF1KE2025
(Linear) (Six-base) MAPSORT of: KIEE2025.seq Check: 940 from: 1 to: 1824
KIEE2025.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 738 1824
Size: 738 1086
AatII G_ACGT'C
Cuts at: 0 875 1824
Size: 875 949
Acc65I G'GTAC_C
Cuts at: 0 938 1244 1824
Size: 938 306 580
Fragments arranged by size:
938 580 306
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1824
Size: 856 968
AflIII A'CryG_T
Cuts at: 0 851 1309 1484 1824
Size: 851 458 175 340
Fragments arranged by size:
851 458 340 175
AlwNI CAG_nnn'CTG
Cuts at: 0 165 1824
Size: 165 1659
ApaI G_GGCC'C
Cuts at: 0 74 1824
Size: 74 1750
ApoI r'AATT_y
Cuts at: 0 1123 1824
Size: 1123 701
AvaI C'yCGr_G
Cuts at: 0 57 172 254 714 742 1824
Size: 57 115 82 460 28 1082
Fragments arranged by size:
1082 460 115 82 57 28
BamHI G'GATC_C
Cuts at: 0 587 1037 1824
Size: 587 450 787
Fragments arranged by size:
787 587 450
BanI G'GyrC_C
Cuts at: 0 100 508 938 982 1244 1256 1693 1824
Size: 100 408 430 44 262 12 437 131
Fragments arranged by size:
437 430 408 262 131 100 44 12
BanII G_rGCy'C
Cuts at: 0 74 773 1232 1657 1824
Size: 74 699 459 425 167
Fragments arranged by size:
699 459 425 167 74
BbeI G_GCGC'C
Cuts at: 0 104 1697 1824
Size: 104 1593 127
Fragments arranged by size:
1593 127 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 422 1824
Size: 422 1402
BbvCI CC'TCA_GC
Cuts at: 0 665 1513 1824
Size: 665 848 311
Fragments arranged by size:
848 665 311
BglI GCCn_nnn'nGGC
Cuts at: 0 278 1824
Size: 278 1546
Bme1580I G_kGCm'C
Cuts at: 0 74 1435 1728 1824
Size: 74 1361 293 96
Fragments arranged by size:
1361 293 96 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 635 826 1566 1824
Size: 415 220 191 740 258
Fragments arranged by size:
740 415 258 220 191
Bpu10I CC'TnA_GC
Cuts at: 0 665 1513 1824
Size: 665 848 311
Fragments arranged by size:
848 665 311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1578 1824
Size: 1578 246
BsaAI yAC'GTr
Cuts at: 0 498 1824
Size: 498 1326
BsaBI GATnn'nnATC
Cuts at: 0 1111 1824
Size: 1111 713
BsaHI Gr'CG_yC
Cuts at: 0 101 872 1694 1780 1824
Size: 101 771 822 86 44
Fragments arranged by size:
822 771 101 86 44
BsaWI w'CCGG_w
Cuts at: 0 1034 1824
Size: 1034 790
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 718 748 1627 1657 1824
Size: 718 30 879 30 167
Fragments arranged by size:
879 718 167 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 439 742 1824
Size: 100 339 303 1082
Fragments arranged by size:
1082 339 303 100
BseYI C'CCAG_C
Cuts at: 0 470 656 841 1824
Size: 470 186 185 983
Fragments arranged by size:
983 470 186 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 460 1824
Size: 288 172 1364
Fragments arranged by size:
1364 288 172
BsiEI CG_ry'CG
Cuts at: 0 1547 1824
Size: 1547 277
BsiHKAI G_wGCw'C
Cuts at: 0 247 621 1669 1824
Size: 247 374 1048 155
Fragments arranged by size:
1048 374 247 155
BsmI GAATG_Cn'
Cuts at: 0 1137 1824
Size: 1137 687
Bsp1286I G_dGCh'C
Cuts at: 0 74 247 621 773 1232 1435 1657 1669
Size: 74 173 374 152 459 203 222 12
Cuts at: 1669 1728 1824
Size: 59 96
Fragments arranged by size:
459 374 222 203 173 152 96 74
59 12
BspEI T'CCGG_A
Cuts at: 0 1034 1824
Size: 1034 790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 385 738 1824
Size: 385 353 1086
Fragments arranged by size:
1086 385 353
BsrBI CCG'CTC
Cuts at: 0 399 417 1824
Size: 399 18 1407
Fragments arranged by size:
1407 399 18
BsrFI r'CCGG_y
Cuts at: 0 184 1375 1824
Size: 184 1191 449
Fragments arranged by size:
1191 449 184
BssHII G'CGCG_C
Cuts at: 0 180 519 1824
Size: 180 339 1305
Fragments arranged by size:
1305 339 180
BssSI C'ACGA_G
Cuts at: 0 1719 1824
Size: 1719 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 165 1824
Size: 165 1659
BstXI CCAn_nnnn'nTGG
Cuts at: 0 457 651 1705 1776 1824
Size: 457 194 1054 71 48
Fragments arranged by size:
1054 457 194 71 48
BstYI r'GATC_y
Cuts at: 0 137 587 1037 1401 1824
Size: 137 450 450 364 423
Fragments arranged by size:
450 450 423 364 137
BtgI C'CryG_G
Cuts at: 0 650 773 954 986 1152 1529 1754 1775
Size: 650 123 181 32 166 377 225 21
Cuts at: 1775 1824
Size: 49
Fragments arranged by size:
650 377 225 181 166 123 49 32
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 189 1824
Size: 189 1635
BtsI GCAGTG_nn'
Cuts at: 0 1569 1710 1824
Size: 1569 141 114
Fragments arranged by size:
1569 141 114
DrdI GACnn_nn'nnGTC
Cuts at: 0 426 1824
Size: 426 1398
EaeI y'GGCC_r
Cuts at: 0 1046 1526 1824
Size: 1046 480 298
Fragments arranged by size:
1046 480 298
EarI CTCTTCn'nnn_
Cuts at: 0 673 793 1824
Size: 673 120 1031
Fragments arranged by size:
1031 673 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 635 826 856 1566 1824
Size: 415 220 191 30 710 258
Fragments arranged by size:
710 415 258 220 191 30
EcoO109I rG'GnC_Cy
Cuts at: 0 70 71 1685 1824
Size: 70 1 1614 139
Fragments arranged by size:
1614 139 70 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 306 948 1824
Size: 306 642 876
Fragments arranged by size:
876 642 306
HaeII r_GCGC'y
Cuts at: 0 104 166 1697 1824
Size: 104 62 1531 127
Fragments arranged by size:
1531 127 104 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 718 750 1285 1317 1617 1649 1824
Size: 718 32 535 32 300 32 175
Fragments arranged by size:
718 535 300 175 32 32 32
HincII GTy'rAC
Cuts at: 0 1740 1824
Size: 1740 84
KasI G'GCGC_C
Cuts at: 0 100 1693 1824
Size: 100 1593 131
Fragments arranged by size:
1593 131 100
KpnI G_GTAC'C
Cuts at: 0 942 1248 1824
Size: 942 306 576
Fragments arranged by size:
942 576 306
MscI TGG'CCA
Cuts at: 0 1048 1824
Size: 1048 776
MslI CAynn'nnrTG
Cuts at: 0 224 1774 1824
Size: 224 1550 50
Fragments arranged by size:
1550 224 50
MspA1I CmG'CkG
Cuts at: 0 7 144 845 988 1520 1824
Size: 7 137 701 143 532 304
Fragments arranged by size:
701 532 304 143 137 7
NaeI GCC'GGC
Cuts at: 0 186 1824
Size: 186 1638
NarI GG'CG_CC
Cuts at: 0 101 1694 1824
Size: 101 1593 130
Fragments arranged by size:
1593 130 101
NcoI C'CATG_G
Cuts at: 0 650 773 954 1754 1775 1824
Size: 650 123 181 800 21 49
Fragments arranged by size:
800 650 181 123 49 21
NgoMIV G'CCGG_C
Cuts at: 0 184 1824
Size: 184 1640
NspI r_CATG'y
Cuts at: 0 786 804 855 1134 1313 1488 1824
Size: 786 18 51 279 179 175 336
Fragments arranged by size:
786 336 279 179 175 51 18
PasI CC'CwG_GG
Cuts at: 0 303 1698 1824
Size: 303 1395 126
Fragments arranged by size:
1395 303 126
PciI A'CATG_T
Cuts at: 0 851 1309 1484 1824
Size: 851 458 175 340
Fragments arranged by size:
851 458 340 175
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 935 960 1158 1824
Size: 393 542 25 198 666
Fragments arranged by size:
666 542 393 198 25
PfoI T'CCnGG_A
Cuts at: 0 1397 1645 1824
Size: 1397 248 179
Fragments arranged by size:
1397 248 179
PpuMI rG'GwC_Cy
Cuts at: 0 1685 1824
Size: 1685 139
PspOMI G'GGCC_C
Cuts at: 0 70 1824
Size: 70 1754
PspXI vC'TCGA_Gb
Cuts at: 0 172 1824
Size: 172 1652
RsrII CG'GwC_CG
Cuts at: 0 696 1824
Size: 696 1128
SacII CC_GC'GG
Cuts at: 0 989 1824
Size: 989 835
SapI GCTCTTCn'nnn_
Cuts at: 0 793 1824
Size: 793 1031
ScaI AGT'ACT
Cuts at: 0 445 1824
Size: 445 1379
SexAI A'CCwGG_T
Cuts at: 0 1247 1824
Size: 1247 577
SfcI C'TryA_G
Cuts at: 0 213 1824
Size: 213 1611
SfoI GGC'GCC
Cuts at: 0 102 1695 1824
Size: 102 1593 129
Fragments arranged by size:
1593 129 102
SmaI CCC'GGG
Cuts at: 0 59 716 1824
Size: 59 657 1108
Fragments arranged by size:
1108 657 59
SmlI C'TyrA_G
Cuts at: 0 172 1557 1824
Size: 172 1385 267
Fragments arranged by size:
1385 267 172
SphI G_CATG'C
Cuts at: 0 804 1824
Size: 804 1020
StyI C'CwwG_G
Cuts at: 0 650 773 923 954 1283 1680 1754 1775
Size: 650 123 150 31 329 397 74 21
Cuts at: 1775 1824
Size: 49
Fragments arranged by size:
650 397 329 150 123 74 49 31
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 354 1654 1824
Size: 354 1300 170
Fragments arranged by size:
1300 354 170
TatI w'GTAC_w
Cuts at: 0 443 1061 1824
Size: 443 618 763
Fragments arranged by size:
763 618 443
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1471 1503 1627 1659 1824
Size: 1471 32 124 32 165
Fragments arranged by size:
1471 165 124 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1395 1705 1824
Size: 1395 310 119
Fragments arranged by size:
1395 310 119
XhoI C'TCGA_G
Cuts at: 0 172 1824
Size: 172 1652
XmaI C'CCGG_G
Cuts at: 0 57 714 1824
Size: 57 657 1110
Fragments arranged by size:
1110 657 57
ZraI GAC'GTC
Cuts at: 0 873 1824
Size: 873 951
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI
Bme1580I BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI
EcoO109I FalI HaeII Hin4I HincII KasI KpnI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PpuMI PspOMI PspXI RsrII SacII
SapI ScaI SexAI SfcI SfoI SmaI SmlI SphI
StyI TaqII TatI TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BsaI BsiWI BsmBI BspHI BsrDI BsrGI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SanDI SbfI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XmnI