(Linear) (Six-base) MAPSORT of: KIEE2307.seq  Check: 4116  from: 1  to: 906



KIEE2307.orf

 With 182 enzymes: SgfI * 

                        November 16, 2010 13:28  ..

AccI GT'mk_AC

Cuts at:      0     426     906 
   Size:        426     480
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757     906 
   Size:        757     149
 
AfeI AGC'GCT

Cuts at:      0     826     906 
   Size:        826      80
 
AflIII A'CryG_T

Cuts at:      0     609     906 
   Size:        609     297
 
AlwNI CAG_nnn'CTG

Cuts at:      0     586     906 
   Size:        586     320
 
ApoI r'AATT_y

Cuts at:      0     280     906 
   Size:        280     626
 
BanI G'GyrC_C

Cuts at:      0     362     906 
   Size:        362     544
 
BanII G_rGCy'C

Cuts at:      0     687     906 
   Size:        687     219
 
BbvCI CC'TCA_GC

Cuts at:      0     726     906 
   Size:        726     180
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     818     906 
   Size:        818      88
 
BclI T'GATC_A

Cuts at:      0     348     906 
   Size:        348     558
 
BglII A'GATC_T

Cuts at:      0     442     906 
   Size:        442     464
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     286     906 
   Size:        286     620
 
Bpu10I CC'TnA_GC

Cuts at:      0     726     906 
   Size:        726     180
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     507     906 
   Size:        507     399
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     489     519     906 
   Size:        489      30     387

  Fragments arranged by size: 

                 489     387      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     510     906 
   Size:        510     396
 
BseYI C'CCAG_C

Cuts at:      0     680     906 
   Size:        680     226
 
Bsp1286I G_dGCh'C

Cuts at:      0     687     906 
   Size:        687     219
 
BssSI C'ACGA_G

Cuts at:      0      41     906 
   Size:         41     865
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     648     906 
   Size:        648     258
 
BstYI r'GATC_y

Cuts at:      0     442     906 
   Size:        442     464
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     387     422     906 
   Size:        387      35     484

  Fragments arranged by size: 

                 484     387      35
 
EaeI y'GGCC_r

Cuts at:      0     170     650     906 
   Size:        170     480     256

  Fragments arranged by size: 

                 480     256     170
 
EarI CTCTTCn'nnn_

Cuts at:      0     399     599     906 
   Size:        399     200     307

  Fragments arranged by size: 

                 399     307     200
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757     906 
   Size:        757     149
 
EcoO109I rG'GnC_Cy

Cuts at:      0     736     906 
   Size:        736     170
 
EcoRI G'AATT_C

Cuts at:      0     280     906 
   Size:        280     626
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     858     906 
   Size:        858      48
 
HaeII r_GCGC'y

Cuts at:      0     828     906 
   Size:        828      78
 
MfeI C'AATT_G

Cuts at:      0     548     704     906 
   Size:        548     156     202

  Fragments arranged by size: 

                 548     202     156
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     906 
   Size:        422     484
 
MscI TGG'CCA

Cuts at:      0     172     652     906 
   Size:        172     480     254

  Fragments arranged by size: 

                 480     254     172
 
NspI r_CATG'y

Cuts at:      0     613     906 
   Size:        613     293
 
PciI A'CATG_T

Cuts at:      0     609     906 
   Size:        609     297
 
PfoI T'CCnGG_A

Cuts at:      0     375     906 
   Size:        375     531
 
PpuMI rG'GwC_Cy

Cuts at:      0     736     906 
   Size:        736     170
 
PshAI GACnn'nnGTC

Cuts at:      0     388     906 
   Size:        388     518
 
SapI GCTCTTCn'nnn_

Cuts at:      0     599     906 
   Size:        599     307
 
SfcI C'TryA_G

Cuts at:      0     587     906 
   Size:        587     319
 
SmlI C'TyrA_G

Cuts at:      0     522     906 
   Size:        522     384
 
SspI AAT'ATT

Cuts at:      0     324     851     906 
   Size:        324     527      55

  Fragments arranged by size: 

                 527     324      55
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     881     906 
   Size:        881      25
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     373     906 
   Size:        373     533
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII    AlwNI     ApoI     BanI    BanII
    BbvCI    BciVI     BclI    BglII     BmrI   Bpu10I    BpuEI    BsaXI
    BseRI    BseYI Bsp1286I    BssSI    BstXI    BstYI    CspCI     EaeI
     EarI  Eco57MI EcoO109I    EcoRI     FalI    HaeII     MfeI     MmeI
     MscI     NspI     PciI     PfoI    PpuMI    PshAI     SapI     SfcI
     SmlI     SspI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI