(Circular) (Six-base) MAPSORT of: pF1KE3344.seq Check: 1732 from: 1 to: 6125 pF1KE3344 6125 bp With 182 enzymes: SgfI * August 14, 2013 11:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1260 2184 1260 Size: 924 5201 AatII G_ACGT'C Cuts at: 404 2822 404 Size: 2418 3707 AccI GT'mk_AC Cuts at: 404 3163 404 Size: 2759 3366 Acc65I G'GTAC_C Cuts at: 3141 3141 Size: 6125 AclI AA'CG_TT Cuts at: 5965 5965 Size: 6125 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 358 403 426 709 814 928 3950 4382 5129 Size: 45 23 283 105 114 3022 432 747 Cuts at: 5129 5397 358 Size: 268 1086 Fragments arranged by size: 3022 1086 747 432 283 268 114 105 45 23 AflIII A'CryG_T Cuts at: 3477 4581 5558 3477 Size: 1104 977 4044 Fragments arranged by size: 4044 1104 977 AgeI A'CCGG_T Cuts at: 4513 4513 Size: 6125 AhdI GACnn_n'nnGTC Cuts at: 879 879 Size: 6125 AleI CACnn'nnGTG Cuts at: 2545 2545 Size: 6125 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 748 748 Size: 6125 AlwNI CAG_nnn'CTG Cuts at: 573 804 858 978 1008 1095 1242 1398 1439 Size: 231 54 120 30 87 147 156 41 Cuts at: 1439 1557 1610 2109 2415 2837 2955 3430 4997 Size: 118 53 499 306 422 118 475 1567 Cuts at: 4997 573 Size: 1701 Fragments arranged by size: 1701 1567 499 475 422 306 231 156 147 120 118 118 87 54 53 41 30 ApaLI G'TGCA_C Cuts at: 2373 4895 2373 Size: 2522 3603 ApoI r'AATT_y Cuts at: 3129 5478 5734 3129 Size: 2349 256 3520 Fragments arranged by size: 3520 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6125 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6125 AvaI C'yCGr_G Cuts at: 344 1635 1905 1938 2130 3116 3145 344 Size: 1291 270 33 192 986 29 3324 Fragments arranged by size: 3324 1291 986 270 192 33 29 BamHI G'GATC_C Cuts at: 1799 3150 1799 Size: 1351 4774 BanI G'GyrC_C Cuts at: 563 1942 2305 2547 2935 2994 3141 3555 3803 Size: 1379 363 242 388 59 147 414 248 Cuts at: 3803 3838 563 Size: 35 2850 Fragments arranged by size: 2850 1379 414 388 363 248 242 147 59 35 BanII G_rGCy'C Cuts at: 1376 1644 1688 1912 1982 2331 2509 3115 3139 Size: 268 44 224 70 349 178 606 24 Cuts at: 3139 4169 1376 Size: 1030 3332 Fragments arranged by size: 3332 1030 606 349 268 224 178 70 44 24 BbeI G_GCGC'C Cuts at: 2998 3559 3807 2998 Size: 561 248 5316 Fragments arranged by size: 5316 561 248 BbsI GAAGACnn'nnnn_ Cuts at: 346 346 Size: 6125 BbvCI CC'TCA_GC Cuts at: 682 682 Size: 6125 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 586 620 586 Size: 34 6091 BciVI GTATCCnnnnn_n' Cuts at: 2283 4023 4784 6125 2283 Size: 1740 761 1341 2283 Fragments arranged by size: 2283 1740 1341 761 BclI T'GATC_A Cuts at: 3645 3645 Size: 6125 BfrBI ATG'CAT Cuts at: 1410 1840 1410 Size: 430 5695 BglI GCCn_nnn'nGGC Cuts at: 313 2122 313 Size: 1809 4316 BglII A'GATC_T Cuts at: 3640 5677 3640 Size: 2037 4088 BlpI GC'TnA_GC Cuts at: 703 2330 3052 3237 703 Size: 1627 722 185 3591 Fragments arranged by size: 3591 1627 722 185 Bme1580I G_kGCm'C Cuts at: 323 1945 2377 2552 2609 2938 3750 3843 4899 Size: 1622 432 175 57 329 812 93 1056 Cuts at: 4899 323 Size: 1549 Fragments arranged by size: 1622 1549 1056 812 432 329 175 93 57 BmgBI CAC'GTC Cuts at: 1493 2220 2880 1493 Size: 727 660 4738 Fragments arranged by size: 4738 727 660 BmrI ACTGGGnnnn_n' Cuts at: 1083 3337 3418 3753 5897 1083 Size: 2254 81 335 2144 1311 Fragments arranged by size: 2254 2144 1311 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1272 4492 1272 Size: 3220 2905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 394 646 988 1509 1636 1867 2020 2035 2086 Size: 252 342 521 127 231 153 15 51 Cuts at: 2086 2131 2307 2493 2727 2977 3051 394 Size: 45 176 186 234 250 74 3468 Fragments arranged by size: 3468 521 342 252 250 234 231 186 176 153 127 74 51 45 15 Bpu10I CC'TnA_GC Cuts at: 682 1655 1804 2629 682 Size: 973 149 825 4178 Fragments arranged by size: 4178 973 825 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1333 2703 2896 3301 4180 4672 4970 1333 Size: 1370 193 405 879 492 298 2488 Fragments arranged by size: 2488 1370 879 492 405 298 193 BsaI GGTCTCn'nnnn_ Cuts at: 463 592 1184 1625 2975 5829 463 Size: 129 592 441 1350 2854 759 Fragments arranged by size: 2854 1350 759 592 441 129 BsaAI yAC'GTr Cuts at: 2516 3478 2516 Size: 962 5163 BsaBI GATnn'nnATC Cuts at: 6 2857 3639 5676 6 Size: 2851 782 2037 455 Fragments arranged by size: 2851 2037 782 455 BsaHI Gr'CG_yC Cuts at: 401 1769 2819 2995 3556 3804 4506 6002 401 Size: 1368 1050 176 561 248 702 1496 524 Fragments arranged by size: 1496 1368 1050 702 561 524 248 176 BsaWI w'CCGG_w Cuts at: 3314 3835 4513 4787 4934 5345 3314 Size: 521 678 274 147 411 4094 Fragments arranged by size: 4094 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 148 178 769 799 2630 2660 148 Size: 30 591 30 1831 30 3613 Fragments arranged by size: 3613 1831 591 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 169 283 286 532 793 907 928 2032 2590 Size: 114 3 246 261 114 21 1104 558 Cuts at: 2590 2638 2707 2959 2989 169 Size: 48 69 252 30 3305 Fragments arranged by size: 3305 1104 558 261 252 246 114 114 69 48 30 21 3 BseYI C'CCAG_C Cuts at: 214 493 1543 2491 2759 3550 4308 4885 5381 Size: 279 1050 948 268 791 758 577 496 Cuts at: 5381 214 Size: 958 Fragments arranged by size: 1050 958 948 791 758 577 496 279 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 265 601 700 2200 2823 2830 265 Size: 336 99 1500 623 7 3560 Fragments arranged by size: 3560 1500 623 336 99 7 BsiEI CG_ry'CG Cuts at: 104 3667 3713 4921 104 Size: 3563 46 1208 1308 Fragments arranged by size: 3563 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 584 1221 1349 1688 2377 2600 3139 3917 4107 Size: 637 128 339 689 223 539 778 190 Cuts at: 4107 4899 584 Size: 792 1810 Fragments arranged by size: 1810 792 778 689 637 539 339 223 190 128 BsiWI C'GTAC_G Cuts at: 198 198 Size: 6125 BsmI GAATG_Cn' Cuts at: 1155 1155 Size: 6125 BsmBI CGTCTCn'nnnn_ Cuts at: 1421 2886 1421 Size: 1465 4660 Bsp1286I G_dGCh'C Cuts at: 323 584 1221 1349 1376 1644 1688 1912 1945 Size: 261 637 128 27 268 44 224 33 Cuts at: 1945 1982 2331 2377 2509 2552 2600 2609 2938 Size: 37 349 46 132 43 48 9 329 Cuts at: 2938 3115 3139 3750 3843 3917 4107 4169 4899 Size: 177 24 611 93 74 190 62 730 Cuts at: 4899 323 Size: 1549 Fragments arranged by size: 1549 730 637 611 349 329 268 261 224 190 177 132 128 93 74 62 48 46 44 43 37 33 27 24 9 BspEI T'CCGG_A Cuts at: 5345 5345 Size: 6125 BspHI T'CATG_A Cuts at: 5301 5301 Size: 6125 BspMI ACCTGCnnnn'nnnn_ Cuts at: 242 1260 2184 3175 3389 3691 4072 242 Size: 1018 924 991 214 302 381 2295 Fragments arranged by size: 2295 1018 991 924 381 302 214 BsrBI CCG'CTC Cuts at: 50 1038 1523 1766 4417 4471 5955 6120 50 Size: 988 485 243 2651 54 1484 165 55 Fragments arranged by size: 2651 1484 988 485 243 165 55 54 BsrDI GCAATG_nn' Cuts at: 732 1840 2199 4037 732 Size: 1108 359 1838 2820 Fragments arranged by size: 2820 1838 1108 359 BsrFI r'CCGG_y Cuts at: 4123 4304 4513 4123 Size: 181 209 5735 Fragments arranged by size: 5735 209 181 BsrGI T'GTAC_A Cuts at: 1559 1559 Size: 6125 BssSI C'ACGA_G Cuts at: 2602 4396 4754 2602 Size: 1794 358 3973 Fragments arranged by size: 3973 1794 358 BstAPI GCAn_nnn'nTGC Cuts at: 804 3502 804 Size: 2698 3427 BstBI TT'CG_AA Cuts at: 4486 4486 Size: 6125 BstEII G'GTnAC_C Cuts at: 414 1812 1899 2763 414 Size: 1398 87 864 3776 Fragments arranged by size: 3776 1398 864 87 BstXI CCAn_nnnn'nTGG Cuts at: 2499 2499 Size: 6125 BstYI r'GATC_y Cuts at: 1 1619 1799 2624 3150 3617 3640 3975 4221 Size: 1618 180 825 526 467 23 335 246 Cuts at: 4221 5233 5319 5331 5677 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1618 1012 825 526 467 449 346 335 246 180 86 23 12 Bsu36I CC'TnA_GG Cuts at: 859 859 Size: 6125 BtgI C'CryG_G Cuts at: 107 1043 1381 2024 2165 107 Size: 936 338 643 141 4067 Fragments arranged by size: 4067 936 643 338 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 348 3057 4075 4256 4419 348 Size: 2709 1018 181 163 2054 Fragments arranged by size: 2709 2054 1018 181 163 BtsI GCAGTG_nn' Cuts at: 2377 3368 2377 Size: 991 5134 DraI TTT'AAA Cuts at: 3124 3124 Size: 6125 DraIII CAC_nnn'GTG Cuts at: 2268 2268 Size: 6125 DrdI GACnn_nn'nnGTC Cuts at: 3831 4689 3831 Size: 858 5267 EaeI y'GGCC_r Cuts at: 593 749 1571 2162 3710 3884 4275 4302 5647 Size: 156 822 591 1548 174 391 27 1345 Cuts at: 5647 593 Size: 1071 Fragments arranged by size: 1548 1345 1071 822 591 391 174 156 27 EagI C'GGCC_G Cuts at: 3710 3710 Size: 6125 EarI CTCTTCn'nnn_ Cuts at: 1073 1105 4148 4358 1073 Size: 32 3043 210 2840 Fragments arranged by size: 3043 2840 210 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 967 1797 1933 3052 4333 4643 4789 5935 967 Size: 830 136 1119 1281 310 146 1146 1157 Fragments arranged by size: 1281 1157 1146 1119 830 310 146 136 EcoICRI GAG'CTC Cuts at: 1686 3137 1686 Size: 1451 4674 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 358 394 403 426 646 709 814 928 988 Size: 36 9 23 220 63 105 114 60 Cuts at: 988 1509 1636 1867 2020 2035 2086 2131 2307 Size: 521 127 231 153 15 51 45 176 Cuts at: 2307 2493 2727 2977 3051 3950 4382 5129 5397 Size: 186 234 250 74 899 432 747 268 Cuts at: 5397 358 Size: 1086 Fragments arranged by size: 1086 899 747 521 432 268 250 234 231 220 186 176 153 127 114 105 74 63 60 51 45 36 23 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 238 514 775 910 982 1807 2017 2353 2472 Size: 276 261 135 72 825 210 336 119 Cuts at: 2472 238 Size: 3891 Fragments arranged by size: 3891 825 336 276 261 210 135 119 72 EcoO109I rG'GnC_Cy Cuts at: 612 2106 2211 2919 3264 612 Size: 1494 105 708 345 3473 Fragments arranged by size: 3473 1494 708 345 105 EcoRI G'AATT_C Cuts at: 3129 3129 Size: 6125 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4144 4144 Size: 6125 FspI TGC'GCA Cuts at: 1306 3906 1306 Size: 2600 3525 HaeII r_GCGC'y Cuts at: 308 2998 3559 3807 4829 5808 308 Size: 2690 561 248 1022 979 625 Fragments arranged by size: 2690 1022 979 625 561 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 865 897 2708 2740 3967 3997 3999 4029 865 Size: 32 1811 32 1227 30 2 30 2961 Fragments arranged by size: 2961 1811 1227 32 32 30 30 2 HincII GTy'rAC Cuts at: 438 2076 2157 2889 3164 438 Size: 1638 81 732 275 3399 Fragments arranged by size: 3399 1638 732 275 81 KasI G'GCGC_C Cuts at: 2994 3555 3803 2994 Size: 561 248 5316 Fragments arranged by size: 5316 561 248 KpnI G_GTAC'C Cuts at: 3145 3145 Size: 6125 MluI A'CGCG_T Cuts at: 5558 5558 Size: 6125 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1457 2465 4796 4980 1457 Size: 1008 2331 184 2602 Fragments arranged by size: 2602 2331 1008 184 MscI TGG'CCA Cuts at: 2164 3886 2164 Size: 1722 4403 MslI CAynn'nnrTG Cuts at: 671 2545 4114 4241 671 Size: 1874 1569 127 2555 Fragments arranged by size: 2555 1874 1569 127 MspA1I CmG'CkG Cuts at: 261 573 624 1323 1395 1610 1713 2756 2826 Size: 312 51 699 72 215 103 1043 70 Cuts at: 2826 2928 3056 3236 3550 3910 4923 5168 261 Size: 102 128 180 314 360 1013 245 1218 Fragments arranged by size: 1218 1043 1013 699 360 314 312 245 215 180 128 103 102 72 70 51 NaeI GCC'GGC Cuts at: 4306 4306 Size: 6125 NarI GG'CG_CC Cuts at: 2995 3556 3804 2995 Size: 561 248 5316 Fragments arranged by size: 5316 561 248 NcoI C'CATG_G Cuts at: 107 2165 107 Size: 2058 4067 NgoMIV G'CCGG_C Cuts at: 4304 4304 Size: 6125 NsiI A_TGCA'T Cuts at: 1412 1842 1412 Size: 430 5695 NspI r_CATG'y Cuts at: 1414 2260 3178 3678 4585 1414 Size: 846 918 500 907 2954 Fragments arranged by size: 2954 918 907 846 500 PasI CC'CwG_GG Cuts at: 2609 2609 Size: 6125 PciI A'CATG_T Cuts at: 4581 4581 Size: 6125 PflMI CCAn_nnn'nTGG Cuts at: 1242 2109 2398 2778 1242 Size: 867 289 380 4589 Fragments arranged by size: 4589 867 380 289 PfoI T'CCnGG_A Cuts at: 371 1622 1997 2102 2354 2729 2813 2954 371 Size: 1251 375 105 252 375 84 141 3542 Fragments arranged by size: 3542 1251 375 375 252 141 105 84 PmeI GTTT'AAAC Cuts at: 3124 3124 Size: 6125 PmlI CAC'GTG Cuts at: 2516 2516 Size: 6125 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1969 2001 5264 5296 1969 Size: 32 3263 32 2798 Fragments arranged by size: 3263 2798 32 32 PpuMI rG'GwC_Cy Cuts at: 2106 2211 2106 Size: 105 6020 PsiI TTA'TAA Cuts at: 2090 2090 Size: 6125 PspXI vC'TCGA_Gb Cuts at: 344 344 Size: 6125 PstI C_TGCA'G Cuts at: 428 515 578 737 776 809 1103 1160 1181 Size: 87 63 159 39 33 294 57 21 Cuts at: 1181 1328 1444 1730 2195 3172 3857 428 Size: 147 116 286 465 977 685 2696 Fragments arranged by size: 2696 977 685 465 294 286 159 147 116 87 63 57 39 33 21 PvuI CG_AT'CG Cuts at: 104 104 Size: 6125 PvuII CAG'CTG Cuts at: 261 573 624 1323 1610 1713 2826 3056 3550 Size: 312 51 699 287 103 1113 230 494 Cuts at: 3550 3910 261 Size: 360 2476 Fragments arranged by size: 2476 1113 699 494 360 312 287 230 103 51 RsrII CG'GwC_CG Cuts at: 4320 4320 Size: 6125 SacI G_AGCT'C Cuts at: 1688 3139 1688 Size: 1451 4674 SalI G'TCGA_C Cuts at: 3162 3162 Size: 6125 SapI GCTCTTCn'nnn_ Cuts at: 4148 4358 4148 Size: 210 5915 SbfI CC_TGCA'GG Cuts at: 515 776 3172 515 Size: 261 2396 3468 Fragments arranged by size: 3468 2396 261 ScaI AGT'ACT Cuts at: 1072 2680 5642 1072 Size: 1608 2962 1555 Fragments arranged by size: 2962 1608 1555 SexAI A'CCwGG_T Cuts at: 1289 1289 Size: 6125 SfcI C'TryA_G Cuts at: 33 424 511 574 720 733 772 805 1099 Size: 391 87 63 146 13 39 33 294 Cuts at: 1099 1134 1156 1177 1324 1440 1726 2191 2923 Size: 35 22 21 147 116 286 465 732 Cuts at: 2923 3168 3853 4846 5037 33 Size: 245 685 993 191 1121 Fragments arranged by size: 1121 993 732 685 465 391 294 286 245 191 147 146 116 87 63 39 35 33 22 21 13 SfoI GGC'GCC Cuts at: 2996 3557 3805 2996 Size: 561 248 5316 Fragments arranged by size: 5316 561 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6125 SmaI CCC'GGG Cuts at: 1637 1907 1940 3147 1637 Size: 270 33 1207 4615 Fragments arranged by size: 4615 1207 270 33 SmlI C'TyrA_G Cuts at: 344 1348 2682 2911 3280 4195 4687 4949 344 Size: 1004 1334 229 369 915 492 262 1520 Fragments arranged by size: 1520 1334 1004 915 492 369 262 229 SpeI A'CTAG_T Cuts at: 67 67 Size: 6125 SphI G_CATG'C Cuts at: 1414 2260 3178 3678 1414 Size: 846 918 500 3861 Fragments arranged by size: 3861 918 846 500 SrfI GCCC'GGGC Cuts at: 1940 1940 Size: 6125 StuI AGG'CCT Cuts at: 2704 2704 Size: 6125 StyI C'CwwG_G Cuts at: 107 245 2165 2444 2652 3259 3613 107 Size: 138 1920 279 208 607 354 2619 Fragments arranged by size: 2619 1920 607 354 279 208 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 496 1960 2751 2818 3708 3841 4300 4509 496 Size: 1464 791 67 890 133 459 209 2112 Fragments arranged by size: 2112 1464 890 791 459 209 133 67 TatI w'GTAC_w Cuts at: 1070 1559 2678 5640 1070 Size: 489 1119 2962 1555 Fragments arranged by size: 2962 1555 1119 489 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 148 180 5642 5674 148 Size: 32 5462 32 599 Fragments arranged by size: 5462 599 32 32 Tth111I GACn'n_nGTC Cuts at: 1291 2833 3922 1291 Size: 1542 1089 3494 Fragments arranged by size: 3494 1542 1089 XbaI T'CTAG_A Cuts at: 3156 3156 Size: 6125 XcmI CCAnnnn_n'nnnnTGG Cuts at: 369 1503 3620 369 Size: 1134 2117 2874 Fragments arranged by size: 2874 2117 1134 XhoI C'TCGA_G Cuts at: 344 344 Size: 6125 XmaI C'CCGG_G Cuts at: 1635 1905 1938 3145 1635 Size: 270 33 1207 4615 Fragments arranged by size: 4615 1207 270 33 ZraI GAC'GTC Cuts at: 402 2820 402 Size: 2418 3707 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AfeI AflII AloI ApaI AscI AvrII BaeI BmtI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PshAI PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SspI SwaI XmnI