################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AfaI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbeI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV FbaI FseI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MseI MunI NarI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccIII 359 TCCGGA T^CCGGA Alw44I 1318 GTGCAC G^TGCAC AlwNI 587 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 951 GGGCCC GGGCC^C ApaLI 1318 GTGCAC G^TGCAC AviII 866 TGCGCA TGC^GCA BalI 1169 TGGCCA TGG^CCA BamHI 261 GGATCC G^GATCC BbrPI 367 CACGTG CAC^GTG BbsI 652 GAAGAC GAAGAC(2/6) BbvCI 385 GCTGAGG CCTCAGC(-5/-2) BglI 275 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 756 GCTCAGC GC^T[ACGT]AGC Bpu1102I 756 GCTCAGC GC^T[ACGT]AGC BpuAI 652 GAAGAC GAAGAC(2/6) BsaI 550 GAGACC GGTCTC(1/5) BsaWI 359 TCCGGA [AT]^CCGG[AT] BseAI 359 TCCGGA T^CCGGA BsmBI 497 GAGACG CGTCTC(1/5) BsmI 1201 GAATGC GAATGC(1/-1) BspEI 359 TCCGGA T^CCGGA BsrDI 777 CATTGC GCAATG(2/0) BstEII 642 GGTCACC G^GT[ACGT]ACC BstPI 642 GGTCACC G^GT[ACGT]ACC BstXI 621 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 261 GGATCC [GA]^GATC[CT] CelII 756 GCTCAGC GC^T[ACGT]AGC DpnII 262 GATC ^GATC EagI 879 CGGCCG C^GGCCG EclXI 879 CGGCCG C^GGCCG Eco52I 879 CGGCCG C^GGCCG EcoO65I 642 GGTCACC G^GT[ACGT]ACC EcoT22I 1111 ATGCAT ATGCA^T FspI 866 TGCGCA TGC^GCA MboI 262 GATC ^GATC MflI 261 GGATCC [GA]^GATC[CT] MluNI 1169 TGGCCA TGG^CCA MroI 359 TCCGGA T^CCGGA MscI 1169 TGGCCA TGG^CCA MslI 405 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 1189 CATATG CA^TATG NdeII 262 GATC ^GATC NsiI 1111 ATGCAT ATGCA^T PmaCI 367 CACGTG CAC^GTG PmlI 367 CACGTG CAC^GTG PpuMI 223 GGGACCC [GA]G^G[AT]CC[CT] PstI 98 CTGCAG CTGCA^G Sau3AI 262 GATC ^GATC SgrAI 87 CGCCGGTG C[GA]^CCGG[CT]G SmaI 278 CCCGGG CCC^GGG SmlI 8 CTCAAG C^T[CT][GA]AG StuI 344 AGGCCT AGG^CCT TaqI 251 TCGA T^CGA Tsp509I 1286 AATT ^AATT TthHB8I 251 TCGA T^CGA XhoII 261 GGATCC [GA]^GATC[CT] XmaCI 278 CCCGGG C^CCGGG XmaI 278 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 262 GATCC GGATC(4/5) 261 GGATC AvaI 57 CCCGAG C^[CT]CG[GA]G 278 CCCGGG BanI 841 GGCACC G^G[CT][GA]CC 1011 GGTGCC BfaI 793 CTAG C^TAG 1033 CTAG BpmI 494 CTGGAG CTGGAG(16/14) 979 CTGGAG BsaAI 367 CACGTG [CT]AC^GT[GA] 1123 TACGTG BseRI 457 GAGGAG GAGGAG(10/8) 982 GAGGAG BsiEI 451 CGGTCG CG[GA][CT]^CG 879 CGGCCG BsmFI 223 GGGAC GGGAC(10/14) 473 GGGAC BsoBI 57 CCCGAG C^[CT]CG[GA]G 278 CCCGGG BssSI 511 CACGAG CACGAG(-5/-1) 1142 CTCGTG DraII 223 GGGACCC [GA]G^G[ACGT]CC[CT] 950 AGGGCCC DraIII 511 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG 967 CACCTTGTG EaeI 879 CGGCCG [CT]^GGCC[GA] 1169 TGGCCA EcoO109I 223 GGGACCC [GA]G^G[ACGT]CC[CT] 950 AGGGCCC HaeII 205 AGCGCC [GA]GCGC^[CT] 216 AGCGCC HinfI 36 GACTC G^A[ACGT]TC 363 GAGTC MaeI 793 CTAG C^TAG 1033 CTAG MaeII 368 ACGT A^CGT 1124 ACGT NaeI 137 GCCGGC GCC^GGC 689 GCCGGC NcoI 1085 CCATGG C^CATGG 1172 CCATGG NgoMIV 137 GCCGGC G^CCGGC 689 GCCGGC PleI 36 GACTC GAGTC(4/5) 363 GAGTC SapI 1399 GAAGAGC GCTCTTC(1/4) 354 GCTCTTC SfcI 98 CTGCAG C^T[GA][CT]AG 899 CTGTAG XcmI 220 CCAGGGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1324 CCAAACTACTTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaII BanII BbvI BcnI BmyI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DsaI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I KspI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PvuII SacII Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI