(Circular) (Six-base) MAPSORT of: pF1KE3196.seq Check: 8214 from: 1 to: 4523 pF1KE3196 4523 bp With 182 enzymes: SgfI * October 24, 2019 20:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1041 1041 Size: 4523 AccI GT'mk_AC Cuts at: 1561 1561 Size: 4523 Acc65I G'GTAC_C Cuts at: 1539 1539 Size: 4523 AclI AA'CG_TT Cuts at: 4363 4363 Size: 4523 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 307 2348 2780 3527 3795 307 Size: 2041 432 747 268 1035 Fragments arranged by size: 2041 1035 747 432 268 AflIII A'CryG_T Cuts at: 1875 2979 3956 1875 Size: 1104 977 2442 Fragments arranged by size: 2442 1104 977 AgeI A'CCGG_T Cuts at: 2911 2911 Size: 4523 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 472 802 973 472 Size: 330 171 4022 Fragments arranged by size: 4022 330 171 AlwNI CAG_nnn'CTG Cuts at: 700 1828 3395 700 Size: 1128 1567 1828 Fragments arranged by size: 1828 1567 1128 ApaI G_GGCC'C Cuts at: 1063 1063 Size: 4523 ApaLI G'TGCA_C Cuts at: 1426 3293 1426 Size: 1867 2656 ApoI r'AATT_y Cuts at: 1527 3876 4132 1527 Size: 2349 256 1918 Fragments arranged by size: 2349 1918 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4523 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4523 AvaI C'yCGr_G Cuts at: 165 386 1543 165 Size: 221 1157 3145 Fragments arranged by size: 3145 1157 221 BamHI G'GATC_C Cuts at: 369 1548 369 Size: 1179 3344 BanI G'GyrC_C Cuts at: 949 1119 1539 1953 2201 2236 949 Size: 170 420 414 248 35 3236 Fragments arranged by size: 3236 420 414 248 170 35 BanII G_rGCy'C Cuts at: 239 638 1063 1358 1469 1514 1537 2567 239 Size: 399 425 295 111 45 23 1030 2195 Fragments arranged by size: 2195 1030 425 399 295 111 45 23 BbeI G_GCGC'C Cuts at: 1957 2205 1957 Size: 248 4275 BbsI GAAGACnn'nnnn_ Cuts at: 767 767 Size: 4523 BbvCI CC'TCA_GC Cuts at: 494 494 Size: 4523 BciVI GTATCCnnnnn_n' Cuts at: 2421 3182 4523 2421 Size: 761 1341 2421 Fragments arranged by size: 2421 1341 761 BclI T'GATC_A Cuts at: 2043 2043 Size: 4523 BfrBI ATG'CAT Cuts at: 1221 1221 Size: 4523 BglI GCCn_nnn'nGGC Cuts at: 389 389 Size: 4523 BglII A'GATC_T Cuts at: 2038 4075 2038 Size: 2037 2486 BlpI GC'TnA_GC Cuts at: 865 1635 865 Size: 770 3753 Bme1580I G_kGCm'C Cuts at: 952 1063 1103 1124 1334 1430 2148 2241 3297 Size: 111 40 21 210 96 718 93 1056 Cuts at: 3297 952 Size: 2178 Fragments arranged by size: 2178 1056 718 210 111 96 93 40 21 BmrI ACTGGGnnnn_n' Cuts at: 663 1735 1816 2151 4295 663 Size: 1072 81 335 2144 891 Fragments arranged by size: 2144 1072 891 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2890 2890 Size: 4523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 623 1108 623 Size: 485 4038 Bpu10I CC'TnA_GC Cuts at: 494 494 Size: 4523 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 101 1699 2578 3070 3368 101 Size: 1598 879 492 298 1256 Fragments arranged by size: 1598 1256 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 652 4227 652 Size: 3575 948 BsaAI yAC'GTr Cuts at: 477 1233 1876 477 Size: 756 643 3124 Fragments arranged by size: 3124 756 643 BsaBI GATnn'nnATC Cuts at: 6 2037 4074 6 Size: 2031 2037 455 Fragments arranged by size: 2037 2031 455 BsaHI Gr'CG_yC Cuts at: 194 257 921 1214 1954 2202 2904 4400 194 Size: 63 664 293 740 248 702 1496 317 Fragments arranged by size: 1496 740 702 664 317 293 248 63 BsaWI w'CCGG_w Cuts at: 467 1712 2233 2911 3185 3332 3743 467 Size: 1245 521 678 274 147 411 1247 Fragments arranged by size: 1247 1245 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 580 1105 580 Size: 525 3998 BseYI C'CCAG_C Cuts at: 570 578 690 1474 1487 1948 2706 3283 3779 Size: 8 112 784 13 461 758 577 496 Cuts at: 3779 570 Size: 1314 Fragments arranged by size: 1314 784 758 577 496 461 112 13 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 499 762 814 1141 1172 1226 1435 499 Size: 263 52 327 31 54 209 3587 Fragments arranged by size: 3587 327 263 209 54 52 31 BsiEI CG_ry'CG Cuts at: 104 562 990 2065 2111 3319 104 Size: 458 428 1075 46 1208 1308 Fragments arranged by size: 1308 1208 1075 458 428 46 BsiHKAI G_wGCw'C Cuts at: 204 398 866 1430 1537 2315 2505 3297 204 Size: 194 468 564 107 778 190 792 1430 Fragments arranged by size: 1430 792 778 564 468 194 190 107 BsmI GAATG_Cn' Cuts at: 1315 1315 Size: 4523 BsmBI CGTCTCn'nnnn_ Cuts at: 599 599 Size: 4523 Bsp1286I G_dGCh'C Cuts at: 204 239 398 638 866 952 1063 1103 1124 Size: 35 159 240 228 86 111 40 21 Cuts at: 1124 1334 1358 1430 1469 1514 1537 2148 2241 Size: 210 24 72 39 45 23 611 93 Cuts at: 2241 2315 2505 2567 3297 204 Size: 74 190 62 730 1430 Fragments arranged by size: 1430 730 611 240 228 210 190 159 111 93 86 74 72 62 45 40 39 35 24 23 21 BspEI T'CCGG_A Cuts at: 467 3743 467 Size: 3276 1247 BspHI T'CATG_A Cuts at: 3699 3699 Size: 4523 BspMI ACCTGCnnnn'nnnn_ Cuts at: 738 827 1041 1573 1787 2089 2470 738 Size: 89 214 532 214 302 381 2791 Fragments arranged by size: 2791 532 381 302 214 214 89 BsrBI CCG'CTC Cuts at: 50 115 531 645 2815 2869 4353 4518 50 Size: 65 416 114 2170 54 1484 165 55 Fragments arranged by size: 2170 1484 416 165 114 65 55 54 BsrDI GCAATG_nn' Cuts at: 884 2435 884 Size: 1551 2972 BsrFI r'CCGG_y Cuts at: 196 245 797 2521 2702 2911 196 Size: 49 552 1724 181 209 1808 Fragments arranged by size: 1808 1724 552 209 181 49 BssSI C'ACGA_G Cuts at: 619 1250 2794 3152 619 Size: 631 1544 358 1990 Fragments arranged by size: 1990 1544 631 358 BstAPI GCAn_nnn'nTGC Cuts at: 1900 1900 Size: 4523 BstBI TT'CG_AA Cuts at: 2884 2884 Size: 4523 BstEII G'GTnAC_C Cuts at: 750 750 Size: 4523 BstXI CCAn_nnnn'nTGG Cuts at: 736 736 Size: 4523 BstYI r'GATC_y Cuts at: 1 369 1548 2015 2038 2373 2619 3631 3717 Size: 368 1179 467 23 335 246 1012 86 Cuts at: 3717 3729 4075 1 Size: 12 346 449 Fragments arranged by size: 1179 1012 467 449 368 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 212 305 352 400 984 1193 1280 1334 212 Size: 93 47 48 584 209 87 54 3401 Fragments arranged by size: 3401 584 209 93 87 54 48 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2473 2654 2817 2473 Size: 181 163 4179 Fragments arranged by size: 4179 181 163 BtsI GCAGTG_nn' Cuts at: 1150 1179 1388 1766 1150 Size: 29 209 378 3907 Fragments arranged by size: 3907 378 209 29 DraI TTT'AAA Cuts at: 1522 1522 Size: 4523 DraIII CAC_nnn'GTG Cuts at: 624 1080 624 Size: 456 4067 DrdI GACnn_nn'nnGTC Cuts at: 2229 3087 2229 Size: 858 3665 EaeI y'GGCC_r Cuts at: 987 1277 2108 2282 2673 2700 4045 987 Size: 290 831 174 391 27 1345 1465 Fragments arranged by size: 1465 1345 831 391 290 174 27 EagI C'GGCC_G Cuts at: 987 2108 987 Size: 1121 3402 EarI CTCTTCn'nnn_ Cuts at: 469 859 1008 1276 1502 2546 2756 469 Size: 390 149 268 226 1044 210 2236 Fragments arranged by size: 2236 1044 390 268 226 210 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 549 2731 3041 3187 4333 549 Size: 2182 310 146 1146 739 Fragments arranged by size: 2182 1146 739 310 146 EcoICRI GAG'CTC Cuts at: 1535 1535 Size: 4523 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 307 623 1108 2348 2780 3527 3795 307 Size: 316 485 1240 432 747 268 1035 Fragments arranged by size: 1240 1035 747 485 432 316 268 EcoO109I rG'GnC_Cy Cuts at: 332 1059 1662 332 Size: 727 603 3193 Fragments arranged by size: 3193 727 603 EcoRI G'AATT_C Cuts at: 1527 1527 Size: 4523 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2542 2542 Size: 4523 FspI TGC'GCA Cuts at: 976 2304 976 Size: 1328 3195 HaeII r_GCGC'y Cuts at: 317 328 1957 2205 3227 4206 317 Size: 11 1629 248 1022 979 634 Fragments arranged by size: 1629 1022 979 634 248 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2365 2395 2397 2427 2365 Size: 30 2 30 4461 Fragments arranged by size: 4461 30 30 2 HincII GTy'rAC Cuts at: 1562 1562 Size: 4523 KasI G'GCGC_C Cuts at: 1953 2201 1953 Size: 248 4275 KpnI G_GTAC'C Cuts at: 1543 1543 Size: 4523 MluI A'CGCG_T Cuts at: 3956 3956 Size: 4523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 542 3194 3378 542 Size: 2652 184 1687 Fragments arranged by size: 2652 1687 184 MscI TGG'CCA Cuts at: 1279 2284 1279 Size: 1005 3518 MslI CAynn'nnrTG Cuts at: 517 2512 2639 517 Size: 1995 127 2401 Fragments arranged by size: 2401 1995 127 MspA1I CmG'CkG Cuts at: 140 345 354 376 402 578 986 1202 1210 Size: 205 9 22 26 176 408 216 8 Cuts at: 1210 1454 1478 1634 1948 2308 3321 3566 140 Size: 244 24 156 314 360 1013 245 1097 Fragments arranged by size: 1097 1013 408 360 314 245 244 216 205 176 156 26 24 22 9 8 NaeI GCC'GGC Cuts at: 247 799 2704 247 Size: 552 1905 2066 Fragments arranged by size: 2066 1905 552 NarI GG'CG_CC Cuts at: 1954 2202 1954 Size: 248 4275 NcoI C'CATG_G Cuts at: 1193 1280 1193 Size: 87 4436 NdeI CA'TA_TG Cuts at: 1298 1298 Size: 4523 NgoMIV G'CCGG_C Cuts at: 245 797 2702 245 Size: 552 1905 2066 Fragments arranged by size: 2066 1905 552 NsiI A_TGCA'T Cuts at: 1223 1223 Size: 4523 NspI r_CATG'y Cuts at: 1576 2076 2983 1576 Size: 500 907 3116 Fragments arranged by size: 3116 907 500 PciI A'CATG_T Cuts at: 2979 2979 Size: 4523 PmeI GTTT'AAAC Cuts at: 1522 1522 Size: 4523 PmlI CAC'GTG Cuts at: 477 477 Size: 4523 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3662 3694 3662 Size: 32 4491 PpuMI rG'GwC_Cy Cuts at: 332 332 Size: 4523 PspOMI G'GGCC_C Cuts at: 1059 1059 Size: 4523 PstI C_TGCA'G Cuts at: 210 1570 2255 210 Size: 1360 685 2478 Fragments arranged by size: 2478 1360 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4523 PvuII CAG'CTG Cuts at: 140 1210 1478 1948 2308 140 Size: 1070 268 470 360 2355 Fragments arranged by size: 2355 1070 470 360 268 RsrII CG'GwC_CG Cuts at: 2718 2718 Size: 4523 SacI G_AGCT'C Cuts at: 1537 1537 Size: 4523 SacII CC_GC'GG Cuts at: 355 403 987 355 Size: 48 584 3891 Fragments arranged by size: 3891 584 48 SalI G'TCGA_C Cuts at: 1560 1560 Size: 4523 SanDI GG'GwC_CC Cuts at: 332 332 Size: 4523 SapI GCTCTTCn'nnn_ Cuts at: 469 1502 2546 2756 469 Size: 1033 1044 210 2236 Fragments arranged by size: 2236 1044 1033 210 SbfI CC_TGCA'GG Cuts at: 1570 1570 Size: 4523 ScaI AGT'ACT Cuts at: 4040 4040 Size: 4523 SfcI C'TryA_G Cuts at: 33 206 1007 1566 2251 3244 3435 33 Size: 173 801 559 685 993 191 1121 Fragments arranged by size: 1121 993 801 685 559 191 173 SfoI GGC'GCC Cuts at: 1955 2203 1955 Size: 248 4275 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4523 SgrAI Cr'CCGG_yG Cuts at: 196 196 Size: 4523 SmaI CCC'GGG Cuts at: 388 1545 388 Size: 1157 3366 SmlI C'TyrA_G Cuts at: 116 1678 2593 3085 3347 116 Size: 1562 915 492 262 1292 Fragments arranged by size: 1562 1292 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4523 SphI G_CATG'C Cuts at: 1576 2076 1576 Size: 500 4023 StuI AGG'CCT Cuts at: 454 454 Size: 4523 StyI C'CwwG_G Cuts at: 1193 1280 1358 1657 2011 1193 Size: 87 78 299 354 3705 Fragments arranged by size: 3705 354 299 87 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 825 1206 1445 2106 2239 2698 2907 825 Size: 381 239 661 133 459 209 2441 Fragments arranged by size: 2441 661 459 381 239 209 133 TatI w'GTAC_w Cuts at: 4038 4038 Size: 4523 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4040 4072 4040 Size: 32 4491 Tth111I GACn'n_nGTC Cuts at: 2320 2320 Size: 4523 XbaI T'CTAG_A Cuts at: 1554 1554 Size: 4523 XcmI CCAnnnn_n'nnnnTGG Cuts at: 335 1439 2018 335 Size: 1104 579 2840 Fragments arranged by size: 2840 1104 579 XmaI C'CCGG_G Cuts at: 386 1543 386 Size: 1157 3366 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BcgI BmgBI BmtI BsaXI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI NruI PacI PasI PflMI PfoI PshAI PsiI PspXI PsrI SexAI SfiI SnaBI SrfI SspI SwaI XhoI XmnI ZraI