Restriction map for pF1KB3462
##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AclI        AcsI        AflII       AgeI        AocI        
ApoI        AscI        AseI        AsnI        AvrII       
BciVI       BfrI        BlnI        BsiWI       BsmI        
BspDI       BspHI       BsrDI       Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      Eco81I      EcoRI       EcoRV       EcoT22I     
FseI        HindIII     HpaI        MfeI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SfuI        SgrAI       SnaBI       SpeI        SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AatII        4519  GACGTC               GACGT^C
Acc65I       6050  GGTACC               G^GTACC
AccI          448  GTCGAC               GT^[AC][GT]AC
AhdI          440  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       5635  GTGCAC               G^TGCAC
ApaLI        5635  GTGCAC               G^TGCAC
Asp718I      6050  GGTACC               G^GTACC
AspEI         440  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         490  TGCGCA               TGC^GCA
BcgI         2712  CGAGAGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         5888  TGATCA               T^GATCA
BspLU11I     5087  ACATGT               A^CATGT
BssHII       2081  GCGCGC               G^CGCGC
DrdI         3709  GACCTGGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         5528  CGGCCG               C^GGCCG
Eam1105I      440  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        5528  CGGCCG               C^GGCCG
Eco52I       5528  CGGCCG               C^GGCCG
FbaI         5888  TGATCA               T^GATCA
FspI          490  TGCGCA               TGC^GCA
KpnI         6050  GGTACC               GGTAC^C
MluI          459  ACGCGT               A^CGCGT
MseI         5690  TTAA                 T^TAA
NcoI         5987  CCATGG               C^CATGG
SalI          448  GTCGAC               G^TCGAC
SapI         5896  GAAGAGC              GCTCTTC(1/4)
Tru9I        5690  TTAA                 T^TAA
Tsp509I      1472  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccIII        620  TCCGGA               T^CCGGA
             3609  TCCGGA              
AflIII        459  ACGCGT               A^C[GA][CT]GT
             5087  ACATGT              
Aor51HI      2448  AGCGCT               AGC^GCT
             5624  AGCGCT              
BbrPI         991  CACGTG               CAC^GTG
             1249  CACGTG              
BfaI         2642  CTAG                 C^TAG
             4213  CTAG                
BsaAI         991  CACGTG               [CT]AC^GT[GA]
             1249  CACGTG              
BsaBI        3108  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5055  GATCTCCATC          
BsaWI         620  TCCGGA               [AT]^CCGG[AT]
             3609  TCCGGA              
BseAI         620  TCCGGA               T^CCGGA
             3609  TCCGGA              
Bsp1407I      683  TGTACA               T^GTACA
             5294  TGTACA              
BspEI         620  TCCGGA               T^CCGGA
             3609  TCCGGA              
BsrGI         683  TGTACA               T^GTACA
             5294  TGTACA              
Eco47III     2448  AGCGCT               AGC^GCT
             5624  AGCGCT              
HincII        448  GTCGAC               GT[CT]^[GA]AC
             3841  GTCAAC              
HindII        448  GTCGAC               GT[CT]^[GA]AC
             3841  GTCAAC              
MaeI         2642  CTAG                 C^TAG
             4213  CTAG                
MamI         3108  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             5055  GATCTCCATC          
MroI          620  TCCGGA               T^CCGGA
             3609  TCCGGA              
PmaCI         991  CACGTG               CAC^GTG
             1249  CACGTG              
PmlI          991  CACGTG               CAC^GTG
             1249  CACGTG              
ScaI         5135  AGTACT               AGT^ACT
             5237  AGTACT              
SexAI         152  ACCAGGT              A^CC[AT]GGT
             4717  ACCTGGT             
SphI          134  GCATGC               GCATG^C
             2104  GCATGC              
SspBI         683  TGTACA               T^GTACA
             5294  TGTACA              
TfiI         3252  GAATC                G^A[AT]TC
             4485  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (  148 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        Asp700I     AspHI       
AspI        AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbeI        BbsI        BbvCI       
BbvI        BcnI        BglI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PaeR7I      PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SacII       Sau3AI      Sau96I      SbfI        
ScrFI       SfaNI       SfcI        SfiI        SfoI        
SmaI        SmlI        Sse8387I    StuI        StyI        
TaqI        TseI        Tsp45I      TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        
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