Restriction map for
pF1KB3462
(Circular) (Six-base) MAPSORT of: pF1KB3462.seq Check: 3164 from: 1 to: 9212
pF1KB3462 9212 bp
With 182 enzymes: SgfI *
January 6, 2012 11:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1728 2420 5426 5904 5918 1728
Size: 692 3006 478 14 5022
Fragments arranged by size:
5022 3006 692 478 14
AatII G_ACGT'C
Cuts at: 4631 4631
Size: 9212
AccI GT'mk_AC
Cuts at: 557 6250 557
Size: 5693 3519
Acc65I G'GTAC_C
Cuts at: 6158 6228 6158
Size: 70 9142
AclI AA'CG_TT
Cuts at: 9052 9052
Size: 9212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 772 1060 1591 1624 2215 2878 3295 3946
Size: 384 288 531 33 591 663 417 651
Cuts at: 3946 7037 7469 8216 8484 388
Size: 3091 432 747 268 1116
Fragments arranged by size:
3091 1116 747 663 651 591 531 432
417 384 288 268 33
AfeI AGC'GCT
Cuts at: 2558 5734 2558
Size: 3176 6036
AflIII A'CryG_T
Cuts at: 567 5195 6564 7668 8645 567
Size: 4628 1369 1104 977 1134
Fragments arranged by size:
4628 1369 1134 1104 977
AgeI A'CCGG_T
Cuts at: 7600 7600
Size: 9212
AhdI GACnn_n'nnGTC
Cuts at: 553 553
Size: 9212
AleI CACnn'nnGTG
Cuts at: 2071 3169 2071
Size: 1098 8114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2443 4198 4627 2443
Size: 1755 429 7028
Fragments arranged by size:
7028 1755 429
AlwNI CAG_nnn'CTG
Cuts at: 222 336 693 780 1152 1353 1370 1401 1522
Size: 114 357 87 372 201 17 31 121
Cuts at: 1522 1569 1743 1833 1842 1974 2547 2727 2739
Size: 47 174 90 9 132 573 180 12
Cuts at: 2739 3390 4341 4902 4938 5793 5925 6021 6517
Size: 651 951 561 36 855 132 96 496
Cuts at: 6517 8084 222
Size: 1567 1350
Fragments arranged by size:
1567 1350 951 855 651 573 561 496
372 357 201 180 174 132 132 121
114 96 90 87 47 36 31 17
12 9
ApaI G_GGCC'C
Cuts at: 1016 1207 1465 1761 1909 3837 4719 4860 5300
Size: 191 258 296 148 1928 882 141 440
Cuts at: 5300 1016
Size: 4928
Fragments arranged by size:
4928 1928 882 440 296 258 191 148
141
ApaLI G'TGCA_C
Cuts at: 5743 7982 5743
Size: 2239 6973
ApoI r'AATT_y
Cuts at: 6216 8565 8821 6216
Size: 2349 256 6607
Fragments arranged by size:
6607 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9212
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9212
AvaI C'yCGr_G
Cuts at: 2639 2894 3583 3898 4204 4453 4763 4832 4859
Size: 255 689 315 306 249 310 69 27
Cuts at: 4859 5179 5263 5292 5560 5888 6232 2639
Size: 320 84 29 268 328 344 5619
Fragments arranged by size:
5619 689 344 328 320 315 310 306
268 255 249 84 69 29 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1056 1089 1056
Size: 33 9179
BamHI G'GATC_C
Cuts at: 3215 3714 4442 6237 3215
Size: 499 728 1795 6190
Fragments arranged by size:
6190 1795 728 499
BanI G'GyrC_C
Cuts at: 998 1213 4313 4781 6158 6228 6642 6890 6925
Size: 215 3100 468 1377 70 414 248 35
Cuts at: 6925 998
Size: 3285
Fragments arranged by size:
3285 3100 1377 468 414 248 215 70
35
BanII G_rGCy'C
Cuts at: 160 1016 1207 1465 1761 1909 2109 2286 2398
Size: 856 191 258 296 148 200 177 112
Cuts at: 2398 2576 2609 2777 2818 2827 3503 3551 3837
Size: 178 33 168 41 9 676 48 286
Cuts at: 3837 3898 3947 4454 4719 4860 4911 5021 5120
Size: 61 49 507 265 141 51 110 99
Cuts at: 5120 5264 5300 5567 5939 6226 7256 160
Size: 144 36 267 372 287 1030 2116
Fragments arranged by size:
2116 1030 856 676 507 372 296 287
286 267 265 258 200 191 178 177
168 148 144 141 112 110 99 61
51 49 48 41 36 33 9
BbeI G_GCGC'C
Cuts at: 1217 4317 4785 6646 6894 1217
Size: 3100 468 1861 248 3535
Fragments arranged by size:
3535 3100 1861 468 248
BbsI GAAGACnn'nnnn_
Cuts at: 3136 3476 4579 4663 4981 3136
Size: 340 1103 84 318 7367
Fragments arranged by size:
7367 1103 340 318 84
BbvCI CC'TCA_GC
Cuts at: 377 443 1343 2941 3571 377
Size: 66 900 1598 630 6018
Fragments arranged by size:
6018 1598 900 630 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2809 2843 2809
Size: 34 9178
BciVI GTATCCnnnnn_n'
Cuts at: 7110 7871 9212 7110
Size: 761 1341 7110
Fragments arranged by size:
7110 1341 761
BclI T'GATC_A
Cuts at: 5996 6732 5996
Size: 736 8476
BglI GCCn_nnn'nGGC
Cuts at: 1211 1817 2551 2860 4759 5518 5695 5809 1211
Size: 606 734 309 1899 759 177 114 4614
Fragments arranged by size:
4614 1899 759 734 606 309 177 114
BglII A'GATC_T
Cuts at: 3701 5060 5162 6727 8764 3701
Size: 1359 102 1565 2037 4149
Fragments arranged by size:
4149 2037 1565 1359 102
BlpI GC'TnA_GC
Cuts at: 121 823 836 1765 3625 3932 4279 4375 4966
Size: 702 13 929 1860 307 347 96 591
Cuts at: 4966 5119 5722 6079 6324 121
Size: 153 603 357 245 3009
Fragments arranged by size:
3009 1860 929 702 603 591 357 347
307 245 153 96 13
Bme1580I G_kGCm'C
Cuts at: 513 567 1016 1207 1465 1761 1822 1909 3837
Size: 54 449 191 258 296 61 87 1928
Cuts at: 3837 4719 4860 5300 5747 6837 6930 7986 513
Size: 882 141 440 447 1090 93 1056 1739
Fragments arranged by size:
1928 1739 1090 1056 882 449 447 440
296 258 191 141 93 87 61 54
BmgBI CAC'GTC
Cuts at: 408 543 1965 3315 408
Size: 135 1422 1350 6305
Fragments arranged by size:
6305 1422 1350 135
BmrI ACTGGGnnnn_n'
Cuts at: 570 1292 1386 5283 5678 6424 6505 6840 8984
Size: 722 94 3897 395 746 81 335 2144
Cuts at: 8984 570
Size: 798
Fragments arranged by size:
3897 2144 798 746 722 395 335 94
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1152 5754 5796 7579 1152
Size: 4602 42 1783 2785
Fragments arranged by size:
4602 2785 1783 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 595 652 787 888 985 1261 2038 2425
Size: 294 57 135 101 97 276 777 387
Cuts at: 2425 2545 2560 2716 2761 2782 2932 2989 3031
Size: 120 15 156 45 21 150 57 42
Cuts at: 3031 3496 3538 3549 3667 3841 4105 4210 4531
Size: 465 42 11 118 174 264 105 321
Cuts at: 4531 4549 4618 4933 5050 5704 6172 301
Size: 18 69 315 117 654 468 3341
Fragments arranged by size:
3341 777 654 468 465 387 321 315
294 276 264 174 156 150 135 120
118 117 105 101 97 69 57 57
45 42 42 21 18 15 11
Bpu10I CC'TnA_GC
Cuts at: 377 443 1343 2392 2941 3211 3571 6121 377
Size: 66 900 1049 549 270 360 2550 3468
Fragments arranged by size:
3468 2550 1049 900 549 360 270 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 412 3316 3730 4348 4458 6388 7267 7759
Size: 39 2904 414 618 110 1930 879 492
Cuts at: 7759 8057 373
Size: 298 1528
Fragments arranged by size:
2904 1930 1528 879 618 492 414 298
110 39
BsaI GGTCTCn'nnnn_
Cuts at: 737 1078 1468 1709 2628 4027 5122 5137 5449
Size: 341 390 241 919 1399 1095 15 312
Cuts at: 5449 8916 737
Size: 3467 1033
Fragments arranged by size:
3467 1399 1095 1033 919 390 341 312
241 15
BsaAI yAC'GTr
Cuts at: 1101 1359 6565 1101
Size: 258 5206 3748
Fragments arranged by size:
5206 3748 258
BsaBI GATnn'nnATC
Cuts at: 6 3220 5167 6726 8763 6
Size: 3214 1947 1559 2037 455
Fragments arranged by size:
3214 2037 1947 1559 455
BsaHI Gr'CG_yC
Cuts at: 677 760 1214 4314 4628 4782 6643 6891 7593
Size: 83 454 3100 314 154 1861 248 702
Cuts at: 7593 9089 677
Size: 1496 800
Fragments arranged by size:
3100 1861 1496 800 702 454 314 248
154 83
BsaWI w'CCGG_w
Cuts at: 728 3717 6401 6922 7600 7874 8021 8432 728
Size: 2989 2684 521 678 274 147 411 1508
Fragments arranged by size:
2989 2684 1508 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2380 2410 3949 3979 5251 5281 6049 6079 2380
Size: 30 1539 30 1272 30 768 30 5513
Fragments arranged by size:
5513 1539 1272 768 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 460 2890 2937 2986 3061 3064 3421 3451 3541
Size: 2430 47 49 75 3 357 30 90
Cuts at: 3541 3571 3588 3601 3604 3792 3906 4209 4222
Size: 30 17 13 3 188 114 303 13
Cuts at: 4222 4279 4312 4372 4783 4786 4852 4966 5128
Size: 57 33 60 411 3 66 114 162
Cuts at: 5128 5293 5323 5404 5460 5974 460
Size: 165 30 81 56 514 3698
Fragments arranged by size:
3698 2430 514 411 357 303 188 165
162 114 114 90 81 75 66 60
57 56 49 47 33 30 30 30
17 13 13 3 3 3
BseYI C'CCAG_C
Cuts at: 190 1088 1509 1633 1826 2434 2446 2864 2948
Size: 898 421 124 193 608 12 418 84
Cuts at: 2948 3005 3206 4815 5003 5449 5548 6014 6637
Size: 57 201 1609 188 446 99 466 623
Cuts at: 6637 7395 7972 8468 190
Size: 758 577 496 934
Fragments arranged by size:
1609 934 898 758 623 608 577 496
466 446 421 418 201 193 188 124
99 84 57 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 795 1178 1339 1414 1477 1987 1998 2095
Size: 338 383 161 75 63 510 11 97
Cuts at: 2095 2362 2920 3130 3619 3697 4243 4354 4495
Size: 267 558 210 489 78 546 111 141
Cuts at: 4495 4705 5643 5938 457
Size: 210 938 295 3731
Fragments arranged by size:
3731 938 558 546 510 489 383 338
295 267 210 210 161 141 111 97
78 75 63 11
BsiEI CG_ry'CG
Cuts at: 104 2295 3921 5639 6754 6800 8008 104
Size: 2191 1626 1718 1115 46 1208 1308
Fragments arranged by size:
2191 1718 1626 1308 1208 1115 46
BsiHKAI G_wGCw'C
Cuts at: 653 917 1488 2210 2398 2411 2576 2777 3947
Size: 264 571 722 188 13 165 201 1170
Cuts at: 3947 4271 4454 5021 5120 5264 5321 5533 5747
Size: 324 183 567 99 144 57 212 214
Cuts at: 5747 5903 6226 7004 7194 7986 653
Size: 156 323 778 190 792 1879
Fragments arranged by size:
1879 1170 792 778 722 571 567 324
323 264 214 212 201 190 188 183
165 156 144 99 57 13
BsmBI CGTCTCn'nnnn_
Cuts at: 277 346 4804 5176 277
Size: 69 4458 372 4313
Fragments arranged by size:
4458 4313 372 69
Bsp1286I G_dGCh'C
Cuts at: 160 513 567 653 917 1016 1207 1465 1488
Size: 353 54 86 264 99 191 258 23
Cuts at: 1488 1761 1822 1909 2109 2210 2286 2398 2411
Size: 273 61 87 200 101 76 112 13
Cuts at: 2411 2576 2609 2777 2818 2827 3503 3551 3837
Size: 165 33 168 41 9 676 48 286
Cuts at: 3837 3898 3947 4271 4454 4719 4860 4911 5021
Size: 61 49 324 183 265 141 51 110
Cuts at: 5021 5120 5264 5300 5321 5533 5567 5747 5903
Size: 99 144 36 21 212 34 180 156
Cuts at: 5903 5939 6226 6837 6930 7004 7194 7256 7986
Size: 36 287 611 93 74 190 62 730
Cuts at: 7986 160
Size: 1386
Fragments arranged by size:
1386 730 676 611 353 324 287 286
273 265 264 258 212 200 191 190
183 180 168 165 156 144 141 112
110 101 99 99 93 87 86 76
74 62 61 61 54 51 49 48
41 36 36 34 33 23 21 13
9
BspEI T'CCGG_A
Cuts at: 728 3717 8432 728
Size: 2989 4715 1508
Fragments arranged by size:
4715 2989 1508
BspHI T'CATG_A
Cuts at: 8388 8388
Size: 9212
BspMI ACCTGCnnnn'nnnn_
Cuts at: 582 1298 1728 1959 2176 2264 2333 2420 3641
Size: 716 430 231 217 88 69 87 1221
Cuts at: 3641 4605 4619 5426 5726 5904 5918 6262 6476
Size: 964 14 807 300 178 14 344 214
Cuts at: 6476 6778 7159 582
Size: 302 381 2635
Fragments arranged by size:
2635 1221 964 807 716 430 381 344
302 300 231 217 214 178 88 87
69 14 14
BsrBI CCG'CTC
Cuts at: 50 270 480 1413 2022 2511 3480 3782 4020
Size: 220 210 933 609 489 969 302 238
Cuts at: 4020 4101 4283 4713 5076 5336 5508 6125 6173
Size: 81 182 430 363 260 172 617 48
Cuts at: 6173 7504 7558 9042 9207 50
Size: 1331 54 1484 165 55
Fragments arranged by size:
1484 1331 969 933 617 609 489 430
363 302 260 238 220 210 182 172
165 81 55 54 48
BsrDI GCAATG_nn'
Cuts at: 7124 7124
Size: 9212
BsrFI r'CCGG_y
Cuts at: 308 1115 4274 4307 4348 5372 5818 7210 7391
Size: 807 3159 33 41 1024 446 1392 181
Cuts at: 7391 7600 308
Size: 209 1920
Fragments arranged by size:
3159 1920 1392 1024 807 446 209 181
41 33
BsrGI T'GTAC_A
Cuts at: 791 5402 791
Size: 4611 4601
BssHII G'CGCG_C
Cuts at: 2189 2189
Size: 9212
BssSI C'ACGA_G
Cuts at: 475 703 928 3697 4066 7483 7841 475
Size: 228 225 2769 369 3417 358 1846
Fragments arranged by size:
3417 2769 1846 369 358 228 225
BstAPI GCAn_nnn'nTGC
Cuts at: 693 933 1353 1401 2218 2727 4341 4902 4938
Size: 240 420 48 817 509 1614 561 36
Cuts at: 4938 6589 693
Size: 1651 3316
Fragments arranged by size:
3316 1651 1614 817 561 509 420 240
48 36
BstBI TT'CG_AA
Cuts at: 7573 7573
Size: 9212
BstEII G'GTnAC_C
Cuts at: 483 3162 3864 4236 4581 5283 483
Size: 2679 702 372 345 702 4412
Fragments arranged by size:
4412 2679 702 702 372 345
BstXI CCAn_nnnn'nTGG
Cuts at: 1617 1782 4882 1617
Size: 165 3100 5947
Fragments arranged by size:
5947 3100 165
BstYI r'GATC_y
Cuts at: 1 2013 2027 2495 3215 3221 3701 3714 4442
Size: 2012 14 468 720 6 480 13 728
Cuts at: 4442 5060 5162 5594 6237 6704 6727 7062 7308
Size: 618 102 432 643 467 23 335 246
Cuts at: 7308 8320 8406 8418 8764 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2012 1012 728 720 643 618 480 468
467 449 432 346 335 246 102 86
23 14 13 12 6
BtgI C'CryG_G
Cuts at: 1004 1901 3234 3845 3957 4145 4409 4469 4538
Size: 897 1333 611 112 188 264 60 69
Cuts at: 4538 5639 6095 1004
Size: 1101 456 4121
Fragments arranged by size:
4121 1333 1101 897 611 456 264 188
112 69 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5767 7162 7343 7506 5767
Size: 1395 181 163 7473
Fragments arranged by size:
7473 1395 181 163
BtsI GCAGTG_nn'
Cuts at: 2624 4936 6455 2624
Size: 2312 1519 5381
Fragments arranged by size:
5381 2312 1519
DraI TTT'AAA
Cuts at: 6211 6211
Size: 9212
DrdI GACnn_nn'nnGTC
Cuts at: 3823 6918 7776 3823
Size: 3095 858 5259
Fragments arranged by size:
5259 3095 858
EaeI y'GGCC_r
Cuts at: 881 1262 1532 1583 1664 3446 3614 4406 5036
Size: 381 270 51 81 1782 168 792 630
Cuts at: 5036 5412 5636 5642 5696 6797 6971 7362 7389
Size: 376 224 6 54 1101 174 391 27
Cuts at: 7389 8734 881
Size: 1345 1359
Fragments arranged by size:
1782 1359 1345 1101 792 630 391 381
376 270 224 174 168 81 54 51
27 6
EagI C'GGCC_G
Cuts at: 5636 6797 5636
Size: 1161 8051
EarI CTCTTCn'nnn_
Cuts at: 286 403 1121 1631 2473 2867 3295 4129 5839
Size: 117 718 510 842 394 428 834 1710
Cuts at: 5839 5999 7235 7445 286
Size: 160 1236 210 2053
Fragments arranged by size:
2053 1710 1236 842 834 718 510 428
394 210 160 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 640 3448 3973 4096 6165 7420 7730 7876 9022
Size: 2808 525 123 2069 1255 310 146 1146
Cuts at: 9022 640
Size: 830
Fragments arranged by size:
2808 2069 1255 1146 830 525 310 146
123
EcoICRI GAG'CTC
Cuts at: 2396 2574 2775 3945 4452 5019 5118 5262 6224
Size: 178 201 1170 507 567 99 144 962
Cuts at: 6224 2396
Size: 5384
Fragments arranged by size:
5384 1170 962 567 507 201 178 144
99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 388 595 652 772 787 888 985 1060
Size: 87 207 57 120 15 101 97 75
Cuts at: 1060 1261 1591 1624 2038 2215 2425 2545 2560
Size: 201 330 33 414 177 210 120 15
Cuts at: 2560 2716 2761 2782 2878 2932 2989 3031 3295
Size: 156 45 21 96 54 57 42 264
Cuts at: 3295 3496 3538 3549 3667 3841 3946 4105 4210
Size: 201 42 11 118 174 105 159 105
Cuts at: 4210 4531 4549 4618 4933 5050 5704 6172 7037
Size: 321 18 69 315 117 654 468 865
Cuts at: 7037 7469 8216 8484 301
Size: 432 747 268 1029
Fragments arranged by size:
1029 865 747 654 468 432 414 330
321 315 268 264 210 207 201 201
177 174 159 156 120 120 118 117
105 105 101 97 96 87 75 69
57 57 54 45 42 42 33 21
18 15 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 370 664 751 859 2074 2539 2689 2961 3583
Size: 294 87 108 1215 465 150 272 622
Cuts at: 3583 3586 4294 4564 5217 370
Size: 3 708 270 653 4365
Fragments arranged by size:
4365 1215 708 653 622 465 294 272
270 150 108 87 3
EcoO109I rG'GnC_Cy
Cuts at: 146 173 333 393 547 1012 1035 1203 1204
Size: 27 160 60 154 465 23 168 1
Cuts at: 1204 1287 1461 1462 1538 1566 1758 1906 2082
Size: 83 174 1 76 28 192 148 176
Cuts at: 2082 2544 2795 2853 2945 3000 3180 3540 3825
Size: 462 251 58 92 55 180 360 285
Cuts at: 3825 4074 4248 4368 4518 4557 4856 5088 5296
Size: 249 174 120 150 39 299 232 208
Cuts at: 5296 5297 5763 5829 5979 6351 146
Size: 1 466 66 150 372 3007
Fragments arranged by size:
3007 466 465 462 372 360 299 285
251 249 232 208 192 180 176 174
174 168 160 154 150 150 148 120
92 83 76 66 60 58 55 39
28 27 23 1 1 1
EcoRI G'AATT_C
Cuts at: 6216 6216
Size: 9212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 414 7231 414
Size: 6817 2395
FspI TGC'GCA
Cuts at: 600 6993 600
Size: 6393 2819
HaeII r_GCGC'y
Cuts at: 830 849 1217 1833 2139 2274 2560 3966 4182
Size: 19 368 616 306 135 286 1406 216
Cuts at: 4182 4317 4785 5736 6646 6894 7916 8895 830
Size: 135 468 951 910 248 1022 979 1147
Fragments arranged by size:
1406 1147 1022 979 951 910 616 468
368 306 286 248 216 135 135 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 539 571 1366 1398 6049 6081 7054 7084 7086
Size: 32 795 32 4651 32 973 30 2
Cuts at: 7086 7116 539
Size: 30 2635
Fragments arranged by size:
4651 2635 973 795 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 558 3951 6251 558
Size: 3393 2300 3519
Fragments arranged by size:
3519 3393 2300
KasI G'GCGC_C
Cuts at: 1213 4313 4781 6642 6890 1213
Size: 3100 468 1861 248 3535
Fragments arranged by size:
3535 3100 1861 468 248
KpnI G_GTAC'C
Cuts at: 6162 6232 6162
Size: 70 9142
MluI A'CGCG_T
Cuts at: 567 8645 567
Size: 8078 1134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1193 7883 8067 1193
Size: 6690 184 2338
Fragments arranged by size:
6690 2338 184
MscI TGG'CCA
Cuts at: 1534 1585 1666 3448 3616 5644 6973 1534
Size: 51 81 1782 168 2028 1329 3773
Fragments arranged by size:
3773 2028 1782 1329 168 81 51
MslI CAynn'nnrTG
Cuts at: 1811 2071 3169 7201 7328 1811
Size: 260 1098 4032 127 3695
Fragments arranged by size:
4032 3695 1098 260 127
MspA1I CmG'CkG
Cuts at: 471 693 822 963 1006 1092 1239 1296 1398
Size: 222 129 141 43 86 147 57 102
Cuts at: 1398 1764 1912 1923 2142 2319 2594 2727 2739
Size: 366 148 11 219 177 275 133 12
Cuts at: 2739 2868 2940 2952 3009 3114 3236 4147 4341
Size: 129 72 12 57 105 122 911 194
Cuts at: 4341 4431 4944 5007 5253 5369 6104 6145 6164
Size: 90 513 63 246 116 735 41 19
Cuts at: 6164 6323 6637 6997 8010 8255 471
Size: 159 314 360 1013 245 1428
Fragments arranged by size:
1428 1013 911 735 513 366 360 314
275 246 245 222 219 194 177 159
148 147 141 133 129 129 122 116
105 102 90 86 72 63 57 57
43 41 19 12 12 11
NaeI GCC'GGC
Cuts at: 4276 4309 4350 5374 7393 4276
Size: 33 41 1024 2019 6095
Fragments arranged by size:
6095 2019 1024 41 33
NarI GG'CG_CC
Cuts at: 1214 4314 4782 6643 6891 1214
Size: 3100 468 1861 248 3535
Fragments arranged by size:
3535 3100 1861 468 248
NcoI C'CATG_G
Cuts at: 6095 6095
Size: 9212
NgoMIV G'CCGG_C
Cuts at: 4274 4307 4348 5372 7391 4274
Size: 33 41 1024 2019 6095
Fragments arranged by size:
6095 2019 1024 41 33
NspI r_CATG'y
Cuts at: 246 2216 5199 5655 6265 6765 7672 246
Size: 1970 2983 456 610 500 907 1786
Fragments arranged by size:
2983 1970 1786 907 610 500 456
PasI CC'CwG_GG
Cuts at: 135 551 3544 135
Size: 416 2993 5803
Fragments arranged by size:
5803 2993 416
PciI A'CATG_T
Cuts at: 5195 7668 5195
Size: 2473 6739
PflMI CCAn_nnn'nTGG
Cuts at: 222 1152 2739 3876 222
Size: 930 1587 1137 5558
Fragments arranged by size:
5558 1587 1137 930
PfoI T'CCnGG_A
Cuts at: 278 401 1121 2030 2237 2561 3554 3683 4382
Size: 123 720 909 207 324 993 129 699
Cuts at: 4382 4445 4700 5084 5171 5321 5989 278
Size: 63 255 384 87 150 668 3501
Fragments arranged by size:
3501 993 909 720 699 668 384 324
255 207 150 129 123 87 63
PmeI GTTT'AAAC
Cuts at: 6211 6211
Size: 9212
PmlI CAC'GTG
Cuts at: 1101 1359 1101
Size: 258 8954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8351 8383 8351
Size: 32 9180
PpuMI rG'GwC_Cy
Cuts at: 146 173 393 547 1287 2795 2945 3000 3180
Size: 27 220 154 740 1508 150 55 180
Cuts at: 3180 3540 3825 4074 4248 4518 4557 5088 5829
Size: 360 285 249 174 270 39 531 741
Cuts at: 5829 146
Size: 3529
Fragments arranged by size:
3529 1508 741 740 531 360 285 270
249 220 180 174 154 150 55 39
27
PspOMI G'GGCC_C
Cuts at: 1012 1203 1461 1757 1905 3833 4715 4856 5296
Size: 191 258 296 148 1928 882 141 440
Cuts at: 5296 1012
Size: 4928
Fragments arranged by size:
4928 1928 882 440 296 258 191 148
141
PspXI vC'TCGA_Gb
Cuts at: 3583 5263 3583
Size: 1680 7532
PstI C_TGCA'G
Cuts at: 302 341 665 860 1097 1295 1406 1565 1838
Size: 39 324 195 237 198 111 159 273
Cuts at: 1838 1922 2114 2135 2228 2471 2552 2939 3077
Size: 84 192 21 93 243 81 387 138
Cuts at: 3077 3401 3761 3992 4172 4616 4853 4895 4943
Size: 324 360 231 180 444 237 42 48
Cuts at: 4943 4949 5258 5915 6026 6259 6944 302
Size: 6 309 657 111 233 685 2570
Fragments arranged by size:
2570 685 657 444 387 360 324 324
309 273 243 237 237 233 231 198
195 192 180 159 138 111 111 93
84 81 48 42 39 21 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9212
PvuII CAG'CTG
Cuts at: 471 693 822 963 1092 1239 1398 1923 2319
Size: 222 129 141 129 147 159 525 396
Cuts at: 2319 2727 2739 2868 2940 3009 3114 4341 4431
Size: 408 12 129 72 69 105 1227 90
Cuts at: 4431 4944 5253 6637 6997 471
Size: 513 309 1384 360 2686
Fragments arranged by size:
2686 1384 1227 525 513 408 396 360
309 222 159 147 141 129 129 129
105 90 72 69 12
RsrII CG'GwC_CG
Cuts at: 7407 7407
Size: 9212
SacI G_AGCT'C
Cuts at: 2398 2576 2777 3947 4454 5021 5120 5264 6226
Size: 178 201 1170 507 567 99 144 962
Cuts at: 6226 2398
Size: 5384
Fragments arranged by size:
5384 1170 962 567 507 201 178 144
99
SacII CC_GC'GG
Cuts at: 1007 3237 4148 1007
Size: 2230 911 6071
Fragments arranged by size:
6071 2230 911
SalI G'TCGA_C
Cuts at: 556 6249 556
Size: 5693 3519
SanDI GG'GwC_CC
Cuts at: 146 173 547 3000 3180 146
Size: 27 374 2453 180 6178
Fragments arranged by size:
6178 2453 374 180 27
SapI GCTCTTCn'nnn_
Cuts at: 5999 7235 7445 5999
Size: 1236 210 7766
Fragments arranged by size:
7766 1236 210
SbfI CC_TGCA'GG
Cuts at: 2552 3077 3992 4616 5915 6026 6259 2552
Size: 525 915 624 1299 111 233 5505
Fragments arranged by size:
5505 1299 915 624 525 233 111
ScaI AGT'ACT
Cuts at: 5245 5347 8729 5245
Size: 102 3382 5728
Fragments arranged by size:
5728 3382 102
SexAI A'CCwGG_T
Cuts at: 260 4825 260
Size: 4565 4647
SfcI C'TryA_G
Cuts at: 33 298 337 661 856 1093 1291 1402 1561
Size: 265 39 324 195 237 198 111 159
Cuts at: 1561 1834 1918 2110 2131 2224 2467 2548 2935
Size: 273 84 192 21 93 243 81 387
Cuts at: 2935 3073 3397 3454 3757 3988 4168 4612 4849
Size: 138 324 57 303 231 180 444 237
Cuts at: 4849 4891 4939 4945 5254 5911 6022 6255 6940
Size: 42 48 6 309 657 111 233 685
Cuts at: 6940 7933 8124 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 657 444 387 324 324
309 303 273 265 243 237 237 233
231 198 195 192 191 180 159 138
111 111 93 84 81 57 48 42
39 21 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2860 4759 5695 2860
Size: 1899 936 6377
Fragments arranged by size:
6377 1899 936
SfoI GGC'GCC
Cuts at: 1215 4315 4783 6644 6892 1215
Size: 3100 468 1861 248 3535
Fragments arranged by size:
3535 3100 1861 468 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9212
SmaI CCC'GGG
Cuts at: 2896 4765 4834 4861 5890 6234 2896
Size: 1869 69 27 1029 344 5874
Fragments arranged by size:
5874 1869 1029 344 69 27
SmlI C'TyrA_G
Cuts at: 388 427 3295 3583 3745 4327 4437 4453 5263
Size: 39 2868 288 162 582 110 16 810
Cuts at: 5263 6367 7282 7774 8036 388
Size: 1104 915 492 262 1564
Fragments arranged by size:
2868 1564 1104 915 810 582 492 288
262 162 110 39 16
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9212
SphI G_CATG'C
Cuts at: 246 2216 6265 6765 246
Size: 1970 4049 500 2693
Fragments arranged by size:
4049 2693 1970 500
StuI AGG'CCT
Cuts at: 2200 2695 5212 5812 2200
Size: 495 2517 600 5600
Fragments arranged by size:
5600 2517 600 495
StyI C'CwwG_G
Cuts at: 152 167 1781 2000 2957 3155 3503 4025 5099
Size: 15 1614 219 957 198 348 522 1074
Cuts at: 5099 6068 6095 6346 6700 152
Size: 969 27 251 354 2664
Fragments arranged by size:
2664 1614 1074 969 957 522 354 348
251 219 198 27 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 229 3181 4255 6795 6928 7387 7596 229
Size: 2952 1074 2540 133 459 209 1845
Fragments arranged by size:
2952 2540 1845 1074 459 209 133
TatI w'GTAC_w
Cuts at: 791 1184 3644 3854 5243 5345 5402 8727 791
Size: 393 2460 210 1389 102 57 3325 1276
Fragments arranged by size:
3325 2460 1389 1276 393 210 102 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3949 3981 5251 5283 5985 6017 8729 8761 3949
Size: 32 1270 32 702 32 2712 32 4400
Fragments arranged by size:
4400 2712 1270 702 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1138 2044 4696 5833 7009 1138
Size: 906 2652 1137 1176 3341
Fragments arranged by size:
3341 2652 1176 1137 906
XbaI T'CTAG_A
Cuts at: 6243 6243
Size: 9212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 219 1899 2007 5652 6707 219
Size: 1680 108 3645 1055 2724
Fragments arranged by size:
3645 2724 1680 1055 108
XhoI C'TCGA_G
Cuts at: 3583 4453 5263 3583
Size: 870 810 7532
Fragments arranged by size:
7532 870 810
XmaI C'CCGG_G
Cuts at: 2894 4763 4832 4859 5888 6232 2894
Size: 1869 69 27 1029 344 5874
Fragments arranged by size:
5874 1869 1029 344 69 27
XmnI GAAnn'nnTTC
Cuts at: 1954 2233 5812 1954
Size: 279 3579 5354
Fragments arranged by size:
5354 3579 279
ZraI GAC'GTC
Cuts at: 4629 4629
Size: 9212
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BfrBI BmtI BsiWI BsmI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PsrI SgrAI SnaBI SrfI SspI
SwaI