Restriction map for pF1KB3462
 (Linear) (Six-base) MAPSORT of: KIBB3462.seq  Check: 7987  from: 1  to: 6099



KIBB3462.orf

 With 182 enzymes: SgfI * 

                        January 6, 2012 11:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1620    2312    5318    5796    5810    6099 
   Size:       1620     692    3006     478      14     289

  Fragments arranged by size: 

                3006    1620     692     478     289      14
 
AatII G_ACGT'C

Cuts at:      0    4523    6099 
   Size:       4523    1576
 
AccI GT'mk_AC

Cuts at:      0     449    6099 
   Size:        449    5650
 
Acc65I G'GTAC_C

Cuts at:      0    6050    6099 
   Size:       6050      49
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     664     952    1483    1516    2107    2770    3187 
   Size:        280     384     288     531      33     591     663     417

Cuts at:   3187    3838    6099 
   Size:        651    2261

  Fragments arranged by size: 

                2261     663     651     591     531     417     384     288
                 280      33
 
AfeI AGC'GCT

Cuts at:      0    2450    5626    6099 
   Size:       2450    3176     473

  Fragments arranged by size: 

                3176    2450     473
 
AflIII A'CryG_T

Cuts at:      0     459    5087    6099 
   Size:        459    4628    1012

  Fragments arranged by size: 

                4628    1012     459
 
AhdI GACnn_n'nnGTC

Cuts at:      0     445    6099 
   Size:        445    5654
 
AleI CACnn'nnGTG

Cuts at:      0    1963    3061    6099 
   Size:       1963    1098    3038

  Fragments arranged by size: 

                3038    1963    1098
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2335    4090    4519    6099 
   Size:       2335    1755     429    1580

  Fragments arranged by size: 

                2335    1755    1580     429
 
AlwNI CAG_nnn'CTG

Cuts at:      0     114     228     585     672    1044    1245    1262    1293 
   Size:        114     114     357      87     372     201      17      31

Cuts at:   1293    1414    1461    1635    1725    1734    1866    2439    2619 
   Size:        121      47     174      90       9     132     573     180

Cuts at:   2619    2631    3282    4233    4794    4830    5685    5817    5913 
   Size:         12     651     951     561      36     855     132      96

Cuts at:   5913    6099 
   Size:        186

  Fragments arranged by size: 

                 951     855     651     573     561     372     357     201
                 186     180     174     132     132     121     114     114
                  96      90      87      47      36      31      17      12
                   9
 
ApaI G_GGCC'C

Cuts at:      0     908    1099    1357    1653    1801    3729    4611    4752 
   Size:        908     191     258     296     148    1928     882     141

Cuts at:   4752    5192    6099 
   Size:        440     907

  Fragments arranged by size: 

                1928     908     907     882     440     296     258     191
                 148     141
 
ApaLI G'TGCA_C

Cuts at:      0    5635    6099 
   Size:       5635     464
 
AvaI C'yCGr_G

Cuts at:      0    2531    2786    3475    3790    4096    4345    4655    4724 
   Size:       2531     255     689     315     306     249     310      69

Cuts at:   4724    4751    5071    5155    5184    5452    5780    6099 
   Size:         27     320      84      29     268     328     319

  Fragments arranged by size: 

                2531     689     328     320     319     315     310     306
                 268     255     249      84      69      29      27
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     948     981    6099 
   Size:        948      33    5118

  Fragments arranged by size: 

                5118     948      33
 
BamHI G'GATC_C

Cuts at:      0    3107    3606    4334    6099 
   Size:       3107     499     728    1765

  Fragments arranged by size: 

                3107    1765     728     499
 
BanI G'GyrC_C

Cuts at:      0     890    1105    4205    4673    6050    6099 
   Size:        890     215    3100     468    1377      49

  Fragments arranged by size: 

                3100    1377     890     468     215      49
 
BanII G_rGCy'C

Cuts at:      0      52     908    1099    1357    1653    1801    2001    2178 
   Size:         52     856     191     258     296     148     200     177

Cuts at:   2178    2290    2468    2501    2669    2710    2719    3395    3443 
   Size:        112     178      33     168      41       9     676      48

Cuts at:   3443    3729    3790    3839    4346    4611    4752    4803    4913 
   Size:        286      61      49     507     265     141      51     110

Cuts at:   4913    5012    5156    5192    5459    5831    6099 
   Size:         99     144      36     267     372     268

  Fragments arranged by size: 

                 856     676     507     372     296     286     268     267
                 265     258     200     191     178     177     168     148
                 144     141     112     110      99      61      52      51
                  49      48      41      36      33       9
 
BbeI G_GCGC'C

Cuts at:      0    1109    4209    4677    6099 
   Size:       1109    3100     468    1422

  Fragments arranged by size: 

                3100    1422    1109     468
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3028    3368    4471    4555    4873    6099 
   Size:       3028     340    1103      84     318    1226

  Fragments arranged by size: 

                3028    1226    1103     340     318      84
 
BbvCI CC'TCA_GC

Cuts at:      0     269     335    1235    2833    3463    6099 
   Size:        269      66     900    1598     630    2636

  Fragments arranged by size: 

                2636    1598     900     630     269      66
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2701    2735    6099 
   Size:       2701      34    3364

  Fragments arranged by size: 

                3364    2701      34
 
BclI T'GATC_A

Cuts at:      0    5888    6099 
   Size:       5888     211
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1103    1709    2443    2752    4651    5410    5587    5701 
   Size:       1103     606     734     309    1899     759     177     114

Cuts at:   5701    6099 
   Size:        398

  Fragments arranged by size: 

                1899    1103     759     734     606     398     309     177
                 114
 
BglII A'GATC_T

Cuts at:      0    3593    4952    5054    6099 
   Size:       3593    1359     102    1045

  Fragments arranged by size: 

                3593    1359    1045     102
 
BlpI GC'TnA_GC

Cuts at:      0      13     715     728    1657    3517    3824    4171    4267 
   Size:         13     702      13     929    1860     307     347      96

Cuts at:   4267    4858    5011    5614    5971    6099 
   Size:        591     153     603     357     128

  Fragments arranged by size: 

                1860     929     702     603     591     357     347     307
                 153     128      96      13      13
 
Bme1580I G_kGCm'C

Cuts at:      0     405     459     908    1099    1357    1653    1714    1801 
   Size:        405      54     449     191     258     296      61      87

Cuts at:   1801    3729    4611    4752    5192    5639    6099 
   Size:       1928     882     141     440     447     460

  Fragments arranged by size: 

                1928     882     460     449     447     440     405     296
                 258     191     141      87      61      54
 
BmgBI CAC'GTC

Cuts at:      0     300     435    1857    3207    6099 
   Size:        300     135    1422    1350    2892

  Fragments arranged by size: 

                2892    1422    1350     300     135
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     462    1184    1278    5175    5570    6099 
   Size:        462     722      94    3897     395     529

  Fragments arranged by size: 

                3897     722     529     462     395      94
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1044    5646    5688    6099 
   Size:       1044    4602      42     411

  Fragments arranged by size: 

                4602    1044     411      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     487     544     679     780     877    1153    1930 
   Size:        193     294      57     135     101      97     276     777

Cuts at:   1930    2317    2437    2452    2608    2653    2674    2824    2881 
   Size:        387     120      15     156      45      21     150      57

Cuts at:   2881    2923    3388    3430    3441    3559    3733    3997    4102 
   Size:         42     465      42      11     118     174     264     105

Cuts at:   4102    4423    4441    4510    4825    4942    5596    6064    6099 
   Size:        321      18      69     315     117     654     468      35

  Fragments arranged by size: 

                 777     654     468     465     387     321     315     294
                 276     264     193     174     156     150     135     120
                 118     117     105     101      97      69      57      57
                  45      42      42      35      21      18      15      11
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     269     335    1235    2284    2833    3103    3463    6013 
   Size:        269      66     900    1049     549     270     360    2550

Cuts at:   6013    6099 
   Size:         86

  Fragments arranged by size: 

                2550    1049     900     549     360     270     269      86
                  66
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     304    3208    3622    4240    4350    6099 
   Size:        265      39    2904     414     618     110    1749

  Fragments arranged by size: 

                2904    1749     618     414     265     110      39
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     629     970    1360    1601    2520    3919    5014    5029 
   Size:        629     341     390     241     919    1399    1095      15

Cuts at:   5029    5341    6099 
   Size:        312     758

  Fragments arranged by size: 

                1399    1095     919     758     629     390     341     312
                 241      15
 
BsaAI yAC'GTr

Cuts at:      0     993    1251    6099 
   Size:        993     258    4848

  Fragments arranged by size: 

                4848     993     258
 
BsaBI GATnn'nnATC

Cuts at:      0    3112    5059    6099 
   Size:       3112    1947    1040

  Fragments arranged by size: 

                3112    1947    1040
 
BsaHI Gr'CG_yC

Cuts at:      0     569     652    1106    4206    4520    4674    6099 
   Size:        569      83     454    3100     314     154    1425

  Fragments arranged by size: 

                3100    1425     569     454     314     154      83
 
BsaWI w'CCGG_w

Cuts at:      0     620    3609    6099 
   Size:        620    2989    2490

  Fragments arranged by size: 

                2989    2490     620
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2272    2302    3841    3871    5143    5173    5941    5971 
   Size:       2272      30    1539      30    1272      30     768      30

Cuts at:   5971    6099 
   Size:        128

  Fragments arranged by size: 

                2272    1539    1272     768     128      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     352    2782    2829    2878    2953    2956    3313    3343 
   Size:        352    2430      47      49      75       3     357      30

Cuts at:   3343    3433    3463    3480    3493    3496    3684    3798    4101 
   Size:         90      30      17      13       3     188     114     303

Cuts at:   4101    4114    4171    4204    4264    4675    4678    4744    4858 
   Size:         13      57      33      60     411       3      66     114

Cuts at:   4858    5020    5185    5215    5296    5352    5866    6099 
   Size:        162     165      30      81      56     514     233

  Fragments arranged by size: 

                2430     514     411     357     352     303     233     188
                 165     162     114     114      90      81      75      66
                  60      57      56      49      47      33      30      30
                  30      17      13      13       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      82     980    1401    1525    1718    2326    2338    2756 
   Size:         82     898     421     124     193     608      12     418

Cuts at:   2756    2840    2897    3098    4707    4895    5341    5440    5906 
   Size:         84      57     201    1609     188     446      99     466

Cuts at:   5906    6099 
   Size:        193

  Fragments arranged by size: 

                1609     898     608     466     446     421     418     201
                 193     193     188     124      99      84      82      57
                  12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     687    1070    1231    1306    1369    1879    1890 
   Size:        349     338     383     161      75      63     510      11

Cuts at:   1890    1987    2254    2812    3022    3511    3589    4135    4246 
   Size:         97     267     558     210     489      78     546     111

Cuts at:   4246    4387    4597    5535    5830    6099 
   Size:        141     210     938     295     269

  Fragments arranged by size: 

                 938     558     546     510     489     383     349     338
                 295     269     267     210     210     161     141     111
                  97      78      75      63      11
 
BsiEI CG_ry'CG

Cuts at:      0    2187    3813    5531    6099 
   Size:       2187    1626    1718     568

  Fragments arranged by size: 

                2187    1718    1626     568
 
BsiHKAI G_wGCw'C

Cuts at:      0     545     809    1380    2102    2290    2303    2468    2669 
   Size:        545     264     571     722     188      13     165     201

Cuts at:   2669    3839    4163    4346    4913    5012    5156    5213    5425 
   Size:       1170     324     183     567      99     144      57     212

Cuts at:   5425    5639    5795    6099 
   Size:        214     156     304

  Fragments arranged by size: 

                1170     722     571     567     545     324     304     264
                 214     212     201     188     183     165     156     144
                  99      57      13
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     169     238    4696    5068    6099 
   Size:        169      69    4458     372    1031

  Fragments arranged by size: 

                4458    1031     372     169      69
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     405     459     545     809     908    1099    1357 
   Size:         52     353      54      86     264      99     191     258

Cuts at:   1357    1380    1653    1714    1801    2001    2102    2178    2290 
   Size:         23     273      61      87     200     101      76     112

Cuts at:   2290    2303    2468    2501    2669    2710    2719    3395    3443 
   Size:         13     165      33     168      41       9     676      48

Cuts at:   3443    3729    3790    3839    4163    4346    4611    4752    4803 
   Size:        286      61      49     324     183     265     141      51

Cuts at:   4803    4913    5012    5156    5192    5213    5425    5459    5639 
   Size:        110      99     144      36      21     212      34     180

Cuts at:   5639    5795    5831    6099 
   Size:        156      36     268

  Fragments arranged by size: 

                 676     353     324     286     273     268     265     264
                 258     212     200     191     183     180     168     165
                 156     144     141     112     110     101      99      99
                  87      86      76      61      61      54      52      51
                  49      48      41      36      36      34      33      23
                  21      13       9
 
BspEI T'CCGG_A

Cuts at:      0     620    3609    6099 
   Size:        620    2989    2490

  Fragments arranged by size: 

                2989    2490     620
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     474    1190    1620    1851    2068    2156    2225    2312 
   Size:        474     716     430     231     217      88      69      87

Cuts at:   2312    3533    4497    4511    5318    5618    5796    5810    6099 
   Size:       1221     964      14     807     300     178      14     289

  Fragments arranged by size: 

                1221     964     807     716     474     430     300     289
                 231     217     178      88      87      69      14      14
            
 
BsrBI CCG'CTC

Cuts at:      0     162     372    1305    1914    2403    3372    3674    3912 
   Size:        162     210     933     609     489     969     302     238

Cuts at:   3912    3993    4175    4605    4968    5228    5400    6017    6065 
   Size:         81     182     430     363     260     172     617      48

Cuts at:   6065    6099 
   Size:         34

  Fragments arranged by size: 

                 969     933     617     609     489     430     363     302
                 260     238     210     182     172     162      81      48
                  34
 
BsrFI r'CCGG_y

Cuts at:      0     200    1007    4166    4199    4240    5264    5710    6099 
   Size:        200     807    3159      33      41    1024     446     389

  Fragments arranged by size: 

                3159    1024     807     446     389     200      41      33
            
 
BsrGI T'GTAC_A

Cuts at:      0     683    5294    6099 
   Size:        683    4611     805

  Fragments arranged by size: 

                4611     805     683
 
BssHII G'CGCG_C

Cuts at:      0    2081    6099 
   Size:       2081    4018
 
BssSI C'ACGA_G

Cuts at:      0     367     595     820    3589    3958    6099 
   Size:        367     228     225    2769     369    2141

  Fragments arranged by size: 

                2769    2141     369     367     228     225
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     585     825    1245    1293    2110    2619    4233    4794 
   Size:        585     240     420      48     817     509    1614     561

Cuts at:   4794    4830    6099 
   Size:         36    1269

  Fragments arranged by size: 

                1614    1269     817     585     561     509     420     240
                  48      36
 
BstEII G'GTnAC_C

Cuts at:      0     375    3054    3756    4128    4473    5175    6099 
   Size:        375    2679     702     372     345     702     924

  Fragments arranged by size: 

                2679     924     702     702     375     372     345
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1509    1674    4774    6099 
   Size:       1509     165    3100    1325

  Fragments arranged by size: 

                3100    1509    1325     165
 
BstYI r'GATC_y

Cuts at:      0    1905    1919    2387    3107    3113    3593    3606    4334 
   Size:       1905      14     468     720       6     480      13     728

Cuts at:   4334    4952    5054    5486    6099 
   Size:        618     102     432     613

  Fragments arranged by size: 

                1905     728     720     618     613     480     468     432
                 102      14      13       6
 
BtgI C'CryG_G

Cuts at:      0     896    1793    3126    3737    3849    4037    4301    4361 
   Size:        896     897    1333     611     112     188     264      60

Cuts at:   4361    4430    5531    5987    6099 
   Size:         69    1101     456     112

  Fragments arranged by size: 

                1333    1101     897     896     611     456     264     188
                 112     112      69      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    5659    6099 
   Size:       5659     440
 
BtsI GCAGTG_nn'

Cuts at:      0    2516    4828    6099 
   Size:       2516    2312    1271

  Fragments arranged by size: 

                2516    2312    1271
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3715    6099 
   Size:       3715    2384
 
EaeI y'GGCC_r

Cuts at:      0     773    1154    1424    1475    1556    3338    3506    4298 
   Size:        773     381     270      51      81    1782     168     792

Cuts at:   4298    4928    5304    5528    5534    5588    6099 
   Size:        630     376     224       6      54     511

  Fragments arranged by size: 

                1782     792     773     630     511     381     376     270
                 224     168      81      54      51       6
 
EagI C'GGCC_G

Cuts at:      0    5528    6099 
   Size:       5528     571
 
EarI CTCTTCn'nnn_

Cuts at:      0     178     295    1013    1523    2365    2759    3187    4021 
   Size:        178     117     718     510     842     394     428     834

Cuts at:   4021    5731    5891    6099 
   Size:       1710     160     208

  Fragments arranged by size: 

                1710     842     834     718     510     428     394     208
                 178     160     117
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     532    3340    3865    3988    6057    6099 
   Size:        532    2808     525     123    2069      42

  Fragments arranged by size: 

                2808    2069     532     525     123      42
 
EcoICRI GAG'CTC

Cuts at:      0    2288    2466    2667    3837    4344    4911    5010    5154 
   Size:       2288     178     201    1170     507     567      99     144

Cuts at:   5154    6099 
   Size:        945

  Fragments arranged by size: 

                2288    1170     945     567     507     201     178     144
                  99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     280     487     544     664     679     780     877 
   Size:        193      87     207      57     120      15     101      97

Cuts at:    877     952    1153    1483    1516    1930    2107    2317    2437 
   Size:         75     201     330      33     414     177     210     120

Cuts at:   2437    2452    2608    2653    2674    2770    2824    2881    2923 
   Size:         15     156      45      21      96      54      57      42

Cuts at:   2923    3187    3388    3430    3441    3559    3733    3838    3997 
   Size:        264     201      42      11     118     174     105     159

Cuts at:   3997    4102    4423    4441    4510    4825    4942    5596    6064 
   Size:        105     321      18      69     315     117     654     468

Cuts at:   6064    6099 
   Size:         35

  Fragments arranged by size: 

                 654     468     414     330     321     315     264     210
                 207     201     201     193     177     174     159     156
                 120     120     118     117     105     105     101      97
                  96      87      75      69      57      57      54      45
                  42      42      35      33      21      18      15      15
                  11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     262     556     643     751    1966    2431    2581    2853 
   Size:        262     294      87     108    1215     465     150     272

Cuts at:   2853    3475    3478    4186    4456    5109    6099 
   Size:        622       3     708     270     653     990

  Fragments arranged by size: 

                1215     990     708     653     622     465     294     272
                 270     262     150     108      87       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0      38      65     225     285     439     904     927    1095 
   Size:         38      27     160      60     154     465      23     168

Cuts at:   1095    1096    1179    1353    1354    1430    1458    1650    1798 
   Size:          1      83     174       1      76      28     192     148

Cuts at:   1798    1974    2436    2687    2745    2837    2892    3072    3432 
   Size:        176     462     251      58      92      55     180     360

Cuts at:   3432    3717    3966    4140    4260    4410    4449    4748    4980 
   Size:        285     249     174     120     150      39     299     232

Cuts at:   4980    5188    5189    5655    5721    5871    6099 
   Size:        208       1     466      66     150     228

  Fragments arranged by size: 

                 466     465     462     360     299     285     251     249
                 232     228     208     192     180     176     174     174
                 168     160     154     150     150     148     120      92
                  83      76      66      60      58      55      39      38
                  28      27      23       1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     306    6099 
   Size:        306    5793
 
FspI TGC'GCA

Cuts at:      0     492    6099 
   Size:        492    5607
 
HaeII r_GCGC'y

Cuts at:      0     722     741    1109    1725    2031    2166    2452    3858 
   Size:        722      19     368     616     306     135     286    1406

Cuts at:   3858    4074    4209    4677    5628    6099 
   Size:        216     135     468     951     471

  Fragments arranged by size: 

                1406     951     722     616     471     468     368     306
                 286     216     135     135      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     431     463    1258    1290    5941    5973    6099 
   Size:        431      32     795      32    4651      32     126

  Fragments arranged by size: 

                4651     795     431     126      32      32      32
 
HincII GTy'rAC

Cuts at:      0     450    3843    6099 
   Size:        450    3393    2256

  Fragments arranged by size: 

                3393    2256     450
 
KasI G'GCGC_C

Cuts at:      0    1105    4205    4673    6099 
   Size:       1105    3100     468    1426

  Fragments arranged by size: 

                3100    1426    1105     468
 
KpnI G_GTAC'C

Cuts at:      0    6054    6099 
   Size:       6054      45
 
MluI A'CGCG_T

Cuts at:      0     459    6099 
   Size:        459    5640
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1085    6099 
   Size:       1085    5014
 
MscI TGG'CCA

Cuts at:      0    1426    1477    1558    3340    3508    5536    6099 
   Size:       1426      51      81    1782     168    2028     563

  Fragments arranged by size: 

                2028    1782    1426     563     168      81      51
 
MslI CAynn'nnrTG

Cuts at:      0    1703    1963    3061    6099 
   Size:       1703     260    1098    3038

  Fragments arranged by size: 

                3038    1703    1098     260
 
MspA1I CmG'CkG

Cuts at:      0     363     585     714     855     898     984    1131    1188 
   Size:        363     222     129     141      43      86     147      57

Cuts at:   1188    1290    1656    1804    1815    2034    2211    2486    2619 
   Size:        102     366     148      11     219     177     275     133

Cuts at:   2619    2631    2760    2832    2844    2901    3006    3128    4039 
   Size:         12     129      72      12      57     105     122     911

Cuts at:   4039    4233    4323    4836    4899    5145    5261    5996    6037 
   Size:        194      90     513      63     246     116     735      41

Cuts at:   6037    6056    6099 
   Size:         19      43

  Fragments arranged by size: 

                 911     735     513     366     363     275     246     222
                 219     194     177     148     147     141     133     129
                 129     122     116     105     102      90      86      72
                  63      57      57      43      43      41      19      12
                  12      11
 
NaeI GCC'GGC

Cuts at:      0    4168    4201    4242    5266    6099 
   Size:       4168      33      41    1024     833

  Fragments arranged by size: 

                4168    1024     833      41      33
 
NarI GG'CG_CC

Cuts at:      0    1106    4206    4674    6099 
   Size:       1106    3100     468    1425

  Fragments arranged by size: 

                3100    1425    1106     468
 
NcoI C'CATG_G

Cuts at:      0    5987    6099 
   Size:       5987     112
 
NgoMIV G'CCGG_C

Cuts at:      0    4166    4199    4240    5264    6099 
   Size:       4166      33      41    1024     835

  Fragments arranged by size: 

                4166    1024     835      41      33
 
NspI r_CATG'y

Cuts at:      0     138    2108    5091    5547    6099 
   Size:        138    1970    2983     456     552

  Fragments arranged by size: 

                2983    1970     552     456     138
 
PasI CC'CwG_GG

Cuts at:      0      27     443    3436    6099 
   Size:         27     416    2993    2663

  Fragments arranged by size: 

                2993    2663     416      27
 
PciI A'CATG_T

Cuts at:      0    5087    6099 
   Size:       5087    1012
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     114    1044    2631    3768    6099 
   Size:        114     930    1587    1137    2331

  Fragments arranged by size: 

                2331    1587    1137     930     114
 
PfoI T'CCnGG_A

Cuts at:      0     170     293    1013    1922    2129    2453    3446    3575 
   Size:        170     123     720     909     207     324     993     129

Cuts at:   3575    4274    4337    4592    4976    5063    5213    5881    6099 
   Size:        699      63     255     384      87     150     668     218

  Fragments arranged by size: 

                 993     909     720     699     668     384     324     255
                 218     207     170     150     129     123      87      63
            
 
PmlI CAC'GTG

Cuts at:      0     993    1251    6099 
   Size:        993     258    4848

  Fragments arranged by size: 

                4848     993     258
 
PpuMI rG'GwC_Cy

Cuts at:      0      38      65     285     439    1179    2687    2837    2892 
   Size:         38      27     220     154     740    1508     150      55

Cuts at:   2892    3072    3432    3717    3966    4140    4410    4449    4980 
   Size:        180     360     285     249     174     270      39     531

Cuts at:   4980    5721    6099 
   Size:        741     378

  Fragments arranged by size: 

                1508     741     740     531     378     360     285     270
                 249     220     180     174     154     150      55      39
                  38      27
 
PspOMI G'GGCC_C

Cuts at:      0     904    1095    1353    1649    1797    3725    4607    4748 
   Size:        904     191     258     296     148    1928     882     141

Cuts at:   4748    5188    6099 
   Size:        440     911

  Fragments arranged by size: 

                1928     911     904     882     440     296     258     191
                 148     141
 
PspXI vC'TCGA_Gb

Cuts at:      0    3475    5155    6099 
   Size:       3475    1680     944

  Fragments arranged by size: 

                3475    1680     944
 
PstI C_TGCA'G

Cuts at:      0     194     233     557     752     989    1187    1298    1457 
   Size:        194      39     324     195     237     198     111     159

Cuts at:   1457    1730    1814    2006    2027    2120    2363    2444    2831 
   Size:        273      84     192      21      93     243      81     387

Cuts at:   2831    2969    3293    3653    3884    4064    4508    4745    4787 
   Size:        138     324     360     231     180     444     237      42

Cuts at:   4787    4835    4841    5150    5807    5918    6099 
   Size:         48       6     309     657     111     181

  Fragments arranged by size: 

                 657     444     387     360     324     324     309     273
                 243     237     237     231     198     195     194     192
                 181     180     159     138     111     111      93      84
                  81      48      42      39      21       6
 
PvuII CAG'CTG

Cuts at:      0     363     585     714     855     984    1131    1290    1815 
   Size:        363     222     129     141     129     147     159     525

Cuts at:   1815    2211    2619    2631    2760    2832    2901    3006    4233 
   Size:        396     408      12     129      72      69     105    1227

Cuts at:   4233    4323    4836    5145    6099 
   Size:         90     513     309     954

  Fragments arranged by size: 

                1227     954     525     513     408     396     363     309
                 222     159     147     141     129     129     129     105
                  90      72      69      12
 
SacI G_AGCT'C

Cuts at:      0    2290    2468    2669    3839    4346    4913    5012    5156 
   Size:       2290     178     201    1170     507     567      99     144

Cuts at:   5156    6099 
   Size:        943

  Fragments arranged by size: 

                2290    1170     943     567     507     201     178     144
                  99
 
SacII CC_GC'GG

Cuts at:      0     899    3129    4040    6099 
   Size:        899    2230     911    2059

  Fragments arranged by size: 

                2230    2059     911     899
 
SalI G'TCGA_C

Cuts at:      0     448    6099 
   Size:        448    5651
 
SanDI GG'GwC_CC

Cuts at:      0      38      65     439    2892    3072    6099 
   Size:         38      27     374    2453     180    3027

  Fragments arranged by size: 

                3027    2453     374     180      38      27
 
SapI GCTCTTCn'nnn_

Cuts at:      0    5891    6099 
   Size:       5891     208
 
SbfI CC_TGCA'GG

Cuts at:      0    2444    2969    3884    4508    5807    5918    6099 
   Size:       2444     525     915     624    1299     111     181

  Fragments arranged by size: 

                2444    1299     915     624     525     181     111
 
ScaI AGT'ACT

Cuts at:      0    5137    5239    6099 
   Size:       5137     102     860

  Fragments arranged by size: 

                5137     860     102
 
SexAI A'CCwGG_T

Cuts at:      0     152    4717    6099 
   Size:        152    4565    1382

  Fragments arranged by size: 

                4565    1382     152
 
SfcI C'TryA_G

Cuts at:      0     190     229     553     748     985    1183    1294    1453 
   Size:        190      39     324     195     237     198     111     159

Cuts at:   1453    1726    1810    2002    2023    2116    2359    2440    2827 
   Size:        273      84     192      21      93     243      81     387

Cuts at:   2827    2965    3289    3346    3649    3880    4060    4504    4741 
   Size:        138     324      57     303     231     180     444     237

Cuts at:   4741    4783    4831    4837    5146    5803    5914    6099 
   Size:         42      48       6     309     657     111     185

  Fragments arranged by size: 

                 657     444     387     324     324     309     303     273
                 243     237     237     231     198     195     192     190
                 185     180     159     138     111     111      93      84
                  81      57      48      42      39      21       6
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2752    4651    5587    6099 
   Size:       2752    1899     936     512

  Fragments arranged by size: 

                2752    1899     936     512
 
SfoI GGC'GCC

Cuts at:      0    1107    4207    4675    6099 
   Size:       1107    3100     468    1424

  Fragments arranged by size: 

                3100    1424    1107     468
 
SmaI CCC'GGG

Cuts at:      0    2788    4657    4726    4753    5782    6099 
   Size:       2788    1869      69      27    1029     317

  Fragments arranged by size: 

                2788    1869    1029     317      69      27
 
SmlI C'TyrA_G

Cuts at:      0     280     319    3187    3475    3637    4219    4329    4345 
   Size:        280      39    2868     288     162     582     110      16

Cuts at:   4345    5155    6099 
   Size:        810     944

  Fragments arranged by size: 

                2868     944     810     582     288     280     162     110
                  39      16
 
SphI G_CATG'C

Cuts at:      0     138    2108    6099 
   Size:        138    1970    3991

  Fragments arranged by size: 

                3991    1970     138
 
StuI AGG'CCT

Cuts at:      0    2092    2587    5104    5704    6099 
   Size:       2092     495    2517     600     395

  Fragments arranged by size: 

                2517    2092     600     495     395
 
StyI C'CwwG_G

Cuts at:      0      44      59    1673    1892    2849    3047    3395    3917 
   Size:         44      15    1614     219     957     198     348     522

Cuts at:   3917    4991    5960    5987    6099 
   Size:       1074     969      27     112

  Fragments arranged by size: 

                1614    1074     969     957     522     348     219     198
                 112      44      27      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     121    3073    4147    6099 
   Size:        121    2952    1074    1952

  Fragments arranged by size: 

                2952    1952    1074     121
 
TatI w'GTAC_w

Cuts at:      0     683    1076    3536    3746    5135    5237    5294    6099 
   Size:        683     393    2460     210    1389     102      57     805

  Fragments arranged by size: 

                2460    1389     805     683     393     210     102      57
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3841    3873    5143    5175    5877    5909    6099 
   Size:       3841      32    1270      32     702      32     190

  Fragments arranged by size: 

                3841    1270     702     190      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1030    1936    4588    5725    6099 
   Size:       1030     906    2652    1137     374

  Fragments arranged by size: 

                2652    1137    1030     906     374
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     111    1791    1899    5544    6099 
   Size:        111    1680     108    3645     555

  Fragments arranged by size: 

                3645    1680     555     111     108
 
XhoI C'TCGA_G

Cuts at:      0    3475    4345    5155    6099 
   Size:       3475     870     810     944

  Fragments arranged by size: 

                3475     944     870     810
 
XmaI C'CCGG_G

Cuts at:      0    2786    4655    4724    4751    5780    6099 
   Size:       2786    1869      69      27    1029     319

  Fragments arranged by size: 

                2786    1869    1029     319      69      27
 
XmnI GAAnn'nnTTC

Cuts at:      0    1846    2125    5704    6099 
   Size:       1846     279    3579     395

  Fragments arranged by size: 

                3579    1846     395     279
 
ZraI GAC'GTC

Cuts at:      0    4521    6099 
   Size:       4521    1578
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PstI    PvuII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AloI     ApoI     AscI     AseI    AsiSI
    AvrII    BciVI    BfrBI     BmtI    BsiWI     BsmI    BspHI    BsrDI
    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII    EcoRI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PmeI     PpiI    PshAI     PsiI
     PsrI     PvuI    RsrII     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     XbaI
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