Restriction map for pF1KB7819
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AviII       AvrII       BamHI       BbrPI       
BbsI        BbvCI       BcgI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaWI       BseAI       BsiWI       BsmBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      Eam1105I    
EarI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HindIII     HpaI        Ksp632I     MaeI        MboII       
MfeI        MflI        MluI        MroI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
Van91I      XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I        593  GGTACC               G^GTACC
Asp718I       593  GGTACC               G^GTACC
AspHI         775  GTGCTC               G[AT]GC[AT]^C
AspI          562  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1122  TGGCCA               TGG^CCA
BciVI        1086  GTATCC               GTATCC(6/5)
BclI          470  TGATCA               T^GATCA
BglI         1376  GCCCTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         468  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          164  GGTCTC               GGTCTC(1/5)
BsgI          613  GTGCAG               GTGCAG(16/14)
BsiHKAI       775  GTGCTC               G[AT]GC[AT]^C
Bsp1407I      992  TGTACA               T^GTACA
BsrBI         600  GAGCGG               CCGCTC(-3/-3)
BsrDI         897  CATTGC               GCAATG(2/0)
BsrFI         250  GCCGGC               [GA]^CCGG[CT]
BsrGI         992  TGTACA               T^GTACA
BstAPI        690  GCACTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        250  GCCGGC               [GA]^CCGG[CT]
DrdI         1031  GACCCAACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI          470  TGATCA               T^GATCA
HincII       1003  GTTGAC               GT[CT]^[GA]AC
HindII       1003  GTTGAC               GT[CT]^[GA]AC
KpnI          593  GGTACC               GGTAC^C
MamI          468  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1122  TGGCCA               TGG^CCA
MscI         1122  TGGCCA               TGG^CCA
MseI          449  TTAA                 T^TAA
NaeI          250  GCCGGC               GCC^GGC
NcoI         1364  CCATGG               C^CATGG
NgoMIV        250  GCCGGC               G^CCGGC
PflFI         562  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SfcI          546  CTACAG               C^T[GA][CT]AG
SphI          638  GCATGC               GCATG^C
SspBI         992  TGTACA               T^GTACA
TaqI         1197  TCGA                 T^CGA
Tru9I         449  TTAA                 T^TAA
Tth111I       562  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1197  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BsiEI         248  CGGCCG               CG[GA][CT]^CG
             1450  CGGCCG              
BsmI          918  GAATGC               GAATGC(1/-1)
             1219  GCATTC              
BssHII        122  GCGCGC               G^CGCGC
              339  GCGCGC              
DpnII         471  GATC                 ^GATC
             1057  GATC                
EagI          248  CGGCCG               C^GGCCG
             1450  CGGCCG              
EclXI         248  CGGCCG               C^GGCCG
             1450  CGGCCG              
Eco52I        248  CGGCCG               C^GGCCG
             1450  CGGCCG              
KspI          287  CCGCGG               CCGC^GG
              383  CCGCGG              
MaeII         563  ACGT                 A^CGT
              659  ACGT                
MboI          471  GATC                 ^GATC
             1057  GATC                
MslI          897  CATTGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1177  CACAGCTATG          
NdeII         471  GATC                 ^GATC
             1057  GATC                
NspI           29  ACATGC               [GA]CATG^[CT]
              638  GCATGC              
PpuMI        1271  AGGTCCT              [GA]G^G[AT]CC[CT]
             1413  GGGTCCC             
PshAI         136  GACGAGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1408  GACCAGGGTC          
PvuII         934  CAGCTG               CAG^CTG
             1335  CAGCTG              
SacII         287  CCGCGG               CCGC^GG
              383  CCGCGG              
Sau3AI        471  GATC                 ^GATC
             1057  GATC                
Tsp509I       942  AATT                 ^AATT
             1043  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
ApaI        AvaI        AvaII       BanI        BanII       
BbeI        BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        MaeIII      
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NlaIII      NlaIV       
PleI        RsaI        Sau96I      ScrFI       SfaNI       
SfoI        SmaI        StyI        TfiI        TseI        
Tsp45I      TspRI       XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com