Restriction map for pF1KB7819
 (Linear) (Six-base) MAPSORT of: KIBB7819.seq  Check: 3151  from: 1  to: 1605



KIBB7819.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

Acc65I G'GTAC_C

Cuts at:      0     593    1605 
   Size:        593    1012
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1595    1605 
   Size:       1595      10
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     628    1605 
   Size:        628     977
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     970    1002    1605 
   Size:        970      32     603

  Fragments arranged by size: 

                 970     603      32
 
ApaI G_GGCC'C

Cuts at:      0    1167    1244    1330    1551    1605 
   Size:       1167      77      86     221      54

  Fragments arranged by size: 

                1167     221      86      77      54
 
AvaI C'yCGr_G

Cuts at:      0     174     354    1169    1605 
   Size:        174     180     815     436

  Fragments arranged by size: 

                 815     436     180     174
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1294    1327    1605 
   Size:       1294      33     278

  Fragments arranged by size: 

                1294     278      33
 
BanI G'GyrC_C

Cuts at:      0      40      75      82     181     189     593    1278    1302 
   Size:         40      35       7      99       8     404     685      24

Cuts at:   1302    1605 
   Size:        303

  Fragments arranged by size: 

                 685     404     303      99      40      35      24       8
                   7
 
BanII G_rGCy'C

Cuts at:      0     350     536    1167    1244    1330    1551    1605 
   Size:        350     186     631      77      86     221      54

  Fragments arranged by size: 

                 631     350     221     186      86      77      54
 
BbeI G_GCGC'C

Cuts at:      0      79      86     185    1605 
   Size:         79       7      99    1420

  Fragments arranged by size: 

                1420      99      79       7
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1097    1605 
   Size:       1097     508
 
BclI T'GATC_A

Cuts at:      0     470    1605 
   Size:        470    1135
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1382    1605 
   Size:       1382     223
 
Bme1580I G_kGCm'C

Cuts at:      0      43     194    1167    1244    1330    1551    1605 
   Size:         43     151     973      77      86     221      54

  Fragments arranged by size: 

                 973     221     151      86      77      54      43
 
BmgBI CAC'GTC

Cuts at:      0     564    1605 
   Size:        564    1041
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     885    1302    1483    1605 
   Size:        885     417     181     122

  Fragments arranged by size: 

                 885     417     181     122
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     550     763    1304    1400    1605 
   Size:        412     138     213     541      96     205

  Fragments arranged by size: 

                 541     412     213     205     138      96
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     170    1605 
   Size:        170    1435
 
BsaBI GATnn'nnATC

Cuts at:      0     472    1605 
   Size:        472    1133
 
BsaHI Gr'CG_yC

Cuts at:      0      76      83     182     293     748    1605 
   Size:         76       7      99     111     455     857

  Fragments arranged by size: 

                 857     455     111      99      76       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     655     685    1519    1549    1605 
   Size:        655      30     834      30      56

  Fragments arranged by size: 

                 834     655      56      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1058    1509    1524    1605 
   Size:       1058     451      15      81

  Fragments arranged by size: 

                1058     451      81      15
 
BseYI C'CCAG_C

Cuts at:      0    1096    1158    1543    1605 
   Size:       1096      62     385      62

  Fragments arranged by size: 

                1096     385      62      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634    1605 
   Size:        634     971
 
BsiEI CG_ry'CG

Cuts at:      0     251    1453    1605 
   Size:        251    1202     152

  Fragments arranged by size: 

                1202     251     152
 
BsiHKAI G_wGCw'C

Cuts at:      0     779    1605 
   Size:        779     826
 
BsmI GAATG_Cn'

Cuts at:      0     924    1219    1605 
   Size:        924     295     386

  Fragments arranged by size: 

                 924     386     295
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     194     350     536     779    1167    1244    1330 
   Size:         43     151     156     186     243     388      77      86

Cuts at:   1330    1551    1605 
   Size:        221      54

  Fragments arranged by size: 

                 388     243     221     186     156     151      86      77
                  54      43
 
BsrBI CCG'CTC

Cuts at:      0     602    1605 
   Size:        602    1003
 
BsrDI GCAATG_nn'

Cuts at:      0     896    1605 
   Size:        896     709
 
BsrFI r'CCGG_y

Cuts at:      0     250    1605 
   Size:        250    1355
 
BsrGI T'GTAC_A

Cuts at:      0     992    1605 
   Size:        992     613
 
BssHII G'CGCG_C

Cuts at:      0     122     339    1605 
   Size:        122     217    1266

  Fragments arranged by size: 

                1266     217     122
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     696    1605 
   Size:        696     909
 
BtgI C'CryG_G

Cuts at:      0     287     383    1364    1605 
   Size:        287      96     981     241

  Fragments arranged by size: 

                 981     287     241      96
 
BtsI GCAGTG_nn'

Cuts at:      0     622    1605 
   Size:        622     983
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1037    1605 
   Size:       1037     568
 
EaeI y'GGCC_r

Cuts at:      0     248     521    1122    1331    1450    1605 
   Size:        248     273     601     209     119     155

  Fragments arranged by size: 

                 601     273     248     209     155     119
 
EagI C'GGCC_G

Cuts at:      0     248    1450    1605 
   Size:        248    1202     155

  Fragments arranged by size: 

                1202     248     155
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     507    1333    1605 
   Size:        507     826     272

  Fragments arranged by size: 

                 826     507     272
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     550     763    1304    1400    1595    1605 
   Size:        412     138     213     541      96     195      10

  Fragments arranged by size: 

                 541     412     213     195     138      96      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1163    1164    1272    1414    1547    1548    1605 
   Size:       1163       1     108     142     133       1      57

  Fragments arranged by size: 

                1163     142     133     108      57       1       1
 
HaeII r_GCGC'y

Cuts at:      0      79      86     185    1605 
   Size:         79       7      99    1420

  Fragments arranged by size: 

                1420      99      79       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1048    1080    1519    1551    1605 
   Size:       1048      32     439      32      54

  Fragments arranged by size: 

                1048     439      54      32      32
 
HincII GTy'rAC

Cuts at:      0    1005    1605 
   Size:       1005     600
 
KasI G'GCGC_C

Cuts at:      0      75      82     181    1605 
   Size:         75       7      99    1424

  Fragments arranged by size: 

                1424      99      75       7
 
KpnI G_GTAC'C

Cuts at:      0     597    1605 
   Size:        597    1008
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     776     884    1605 
   Size:        776     108     721

  Fragments arranged by size: 

                 776     721     108
 
MscI TGG'CCA

Cuts at:      0    1124    1605 
   Size:       1124     481
 
MslI CAynn'nnrTG

Cuts at:      0     901    1181    1605 
   Size:        901     280     424

  Fragments arranged by size: 

                 901     424     280
 
MspA1I CmG'CkG

Cuts at:      0     289     385     936    1337    1605 
   Size:        289      96     551     401     268

  Fragments arranged by size: 

                 551     401     289     268      96
 
NaeI GCC'GGC

Cuts at:      0     252    1605 
   Size:        252    1353
 
NarI GG'CG_CC

Cuts at:      0      76      83     182    1605 
   Size:         76       7      99    1423

  Fragments arranged by size: 

                1423      99      76       7
 
NcoI C'CATG_G

Cuts at:      0    1364    1605 
   Size:       1364     241
 
NgoMIV G'CCGG_C

Cuts at:      0     250    1605 
   Size:        250    1355
 
NspI r_CATG'y

Cuts at:      0      33     642    1605 
   Size:         33     609     963

  Fragments arranged by size: 

                 963     609      33
 
PasI CC'CwG_GG

Cuts at:      0    1237    1605 
   Size:       1237     368
 
PfoI T'CCnGG_A

Cuts at:      0     971    1605 
   Size:        971     634
 
PpuMI rG'GwC_Cy

Cuts at:      0    1272    1414    1605 
   Size:       1272     142     191

  Fragments arranged by size: 

                1272     191     142
 
PshAI GACnn'nnGTC

Cuts at:      0     140    1412    1605 
   Size:        140    1272     193

  Fragments arranged by size: 

                1272     193     140
 
PsiI TTA'TAA

Cuts at:      0    1589    1605 
   Size:       1589      16
 
PspOMI G'GGCC_C

Cuts at:      0    1163    1240    1326    1547    1605 
   Size:       1163      77      86     221      58

  Fragments arranged by size: 

                1163     221      86      77      58
 
PvuII CAG'CTG

Cuts at:      0     936    1337    1605 
   Size:        936     401     268

  Fragments arranged by size: 

                 936     401     268
 
SacII CC_GC'GG

Cuts at:      0     290     386    1605 
   Size:        290      96    1219

  Fragments arranged by size: 

                1219     290      96
 
SanDI GG'GwC_CC

Cuts at:      0    1414    1605 
   Size:       1414     191
 
SfcI C'TryA_G

Cuts at:      0     546    1605 
   Size:        546    1059
 
SfoI GGC'GCC

Cuts at:      0      77      84     183    1605 
   Size:         77       7      99    1422

  Fragments arranged by size: 

                1422      99      77       7
 
SmaI CCC'GGG

Cuts at:      0     176     356    1171    1605 
   Size:        176     180     815     434

  Fragments arranged by size: 

                 815     434     180     176
 
SphI G_CATG'C

Cuts at:      0     642    1605 
   Size:        642     963
 
StyI C'CwwG_G

Cuts at:      0     185     213    1047    1364    1421    1605 
   Size:        185      28     834     317      57     184

  Fragments arranged by size: 

                 834     317     185     184      57      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     600    1605 
   Size:        600    1005
 
TatI w'GTAC_w

Cuts at:      0     992    1605 
   Size:        992     613
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     655     687    1605 
   Size:        655      32     918

  Fragments arranged by size: 

                 918     655      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     565    1605 
   Size:        565    1040
 
XmaI C'CCGG_G

Cuts at:      0     174     354    1169    1605 
   Size:        174     180     815     436

  Fragments arranged by size: 

                 815     436     180     174
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI     AloI     ApaI     AvaI     BaeI     BanI
    BanII     BbeI    BciVI     BclI     BglI Bme1580I    BmgBI     BmrI
     BpmI     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI     BtgI     BtsI     DrdI     EaeI     EagI     EciI
  Eco57MI EcoO109I    HaeII    Hin4I   HincII     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PfoI    PpuMI    PshAI     PsiI   PspOMI    PvuII    SacII
    SanDI     SfcI     SfoI     SmaI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII    BamHI     BbsI    BbvCI     BcgI    BfrBI    BglII     BlpI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaWI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EarI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     XbaI     XcmI     XhoI     XmnI
     ZraI
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