Restriction map for pF1KE1989
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        AlwNI       AocI        
ApaI        ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BbrPI       
BbvCI       BciVI       BfrI        BlnI        BsaBI       
BsaWI       BseAI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrGI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraII       DraIII      
Eam1105I    Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT22I     HincII      HindII      HindIII     
HinfI       HpaI        KpnI        KspI        MamI        
MfeI        MroI        MseI        MunI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SbfI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp509I     Tth111I     
XbaI        XhoI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflIII        721  ACGCGT               A^C[GA][CT]GT
Asp700I       375  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1379  TGCGCA               TGC^GCA
BamHI         740  GGATCC               G^GATCC
BanII         476  GAGCTC               G[GA]GC[CT]^C
BbeI          433  GGCGCC               GGCGC^C
BbsI         1390  GTCTTC               GAAGAC(2/6)
BcgI          140  GCACGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1565  TGATCA               T^GATCA
BglII         443  AGATCT               A^GATCT
BlpI         1191  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1191  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1390  GTCTTC               GAAGAC(2/6)
BsaAI         792  CACGTA               [CT]AC^GT[GA]
BsaI         1684  GAGACC               GGTCTC(1/5)
BseRI         715  GAGGAG               GAGGAG(10/8)
BsiEI        1139  CGGCCG               CG[GA][CT]^CG
BsmBI         718  GAGACG               CGTCTC(1/5)
BsmFI         510  GGGAC                GGGAC(10/14)
BstEII        940  GGTTACC              G^GT[ACGT]ACC
BstPI         940  GGTTACC              G^GT[ACGT]ACC
CelII        1191  GCTGAGC              GC^T[ACGT]AGC
DrdI         1549  GACTTCACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1139  CGGCCG               C^GGCCG
EclXI        1139  CGGCCG               C^GGCCG
Eco52I       1139  CGGCCG               C^GGCCG
EcoO65I       940  GGTTACC              G^GT[ACGT]ACC
FbaI         1565  TGATCA               T^GATCA
FseI           62  GGCCGGCC             GGCCGG^CC
FspI         1379  TGCGCA               TGC^GCA
KasI          433  GGCGCC               G^GCGCC
MluI          721  ACGCGT               A^CGCGT
NarI          433  GGCGCC               GG^CGCC
NheI         1248  GCTAGC               G^CTAGC
PflMI        1025  CCATCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          514  CTGCAG               CTGCA^G
SacI          476  GAGCTC               GAGCT^C
ScaI         1109  AGTACT               AGT^ACT
SfcI          514  CTGCAG               C^T[GA][CT]AG
SfoI          433  GGCGCC               GGC^GCC
SmlI          613  CTCAAG               C^T[CT][GA]AG
Tsp45I        787  GTCAC                ^GT[GC]AC
Van91I       1025  CCATCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          375  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          802  GTAGAC               GT^[AC][GT]AC
             1558  GTCTAC              
AcyI          433  GGCGCC               G[GA]^CG[CT]C
             1387  GGCGTC              
AvaII         102  GGACC                G^G[AT]CC
             1227  GGACC               
BfaI          211  CTAG                 C^TAG
             1249  CTAG                
BsaHI         433  GGCGCC               G[GA]^CG[CT]C
             1387  GGCGTC              
BsmAI         718  GAGAC                GTCTC(1/5)
             1684  GAGAC               
BspMI         678  ACCTGC               ACCTGC(4/8)
             1187  ACCTGC              
BstXI         173  CCAACCTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              791  CCACGTACTTGG        
EcoT14I       863  CCATGG               C^C[AT][AT]GG
             1166  CCATGG              
Hin1I         433  GGCGCC               G[GA]^CG[CT]C
             1387  GGCGTC              
MaeI          211  CTAG                 C^TAG
             1249  CTAG                
MaeIII        787  GTCAC                ^GT[ACGT]AC
              941  GTTAC               
NcoI          863  CCATGG               C^CATGG
             1166  CCATGG              
NspI          806  ACATGC               [GA]CATG^[CT]
             1538  GCATGT              
SapI         1257  GCTCTTC              GCTCTTC(1/4)
             1311  GCTCTTC             
SexAI         748  ACCTGGT              A^CC[AT]GGT
             1051  ACCTGGT             
SmaI          537  CCCGGG               CCC^GGG
              546  CCCGGG              
StyI          863  CCATGG               C^C[AT][AT]GG
             1166  CCATGG              
TaqI         1094  TCGA                 T^CGA
             1178  TCGA                
TthHB8I      1094  TCGA                 T^CGA
             1178  TCGA                
XmaCI         537  CCCGGG               C^CCGGG
              546  CCCGGG              
XmaI          537  CCCGGG               C^CCGGG
              546  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        Alw44I      
AlwI        Aor51HI     ApaLI       AspHI       AvaI        
BalI        BanI        BbvI        BcnI        BglI        
BmyI        BpmI        BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsoBI       Bsp1286I    BspHI       
BsrBI       BsrFI       BsrI        BssHII      BssKI       
BssSI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DsaI        EaeI        EarI        Eco47III    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HpaII       HphI        ItaI        Ksp632I     MaeII       
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfiI        
StuI        TseI        TspRI       XcmI        XhoII       
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