Restriction map for pF1KE4378
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvrII       BamHI       BanII       BbeI        BbrPI       
BciVI       BclI        BfaI        BfrI        BlnI        
BpmI        BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsmBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        Tru9I       XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccI          124  GTCTAC               GT^[AC][GT]AC
AccII         521  CGCG                 CG^CG
AflIII        472  ACGTGT               A^C[GA][CT]GT
AlwI          105  GATCC                GGATC(4/5)
AlwNI         387  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1095  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         355  GAGCAC               G[AT]GC[AT]^C
AspI          265  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          171  CTCGAG               C^[CT]CG[GA]G
AviII         349  TGCGCA               TGC^GCA
BalI         1031  TGGCCA               TGG^CCA
BanI          491  GGTGCC               G^G[CT][GA]CC
BbvCI        1111  GCTGAGG              CCTCAGC(-5/-2)
BcgI          761  GCATCACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          968  CCGGG                CC^[GC]GG
BglI         1071  GCCTAAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         479  AGATCT               A^GATCT
BlpI         1135  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1135  GCTGAGC              GC^T[ACGT]AGC
BsgI          667  CTGCAC               GTGCAG(16/14)
BsiHKAI       355  GAGCAC               G[AT]GC[AT]^C
BsiWI         798  CGTACG               C^GTACG
BsmAI         193  GTCTC                GTCTC(1/5)
BsmI          627  GAATGC               GAATGC(1/-1)
BsoBI         171  CTCGAG               C^[CT]CG[GA]G
BspHI         305  TCATGA               T^CATGA
BsrBI         174  GAGCGG               CCGCTC(-3/-3)
BstUI         521  CGCG                 CG^CG
BstXI         225  CCAGCTCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         479  AGATCT               [GA]^GATC[CT]
CelII        1135  GCTGAGC              GC^T[ACGT]AGC
DraII         209  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII        886  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      209  AGGGCCT              [GA]G^G[ACGT]CC[CT]
FspI          349  TGCGCA               TGC^GCA
HapII         968  CCGG                 C^CGG
HincII        598  GTCAAC               GT[CT]^[GA]AC
HindII        598  GTCAAC               GT[CT]^[GA]AC
HpaII         968  CCGG                 C^CGG
MflI          479  AGATCT               [GA]^GATC[CT]
MluNI        1031  TGGCCA               TGG^CCA
MscI         1031  TGGCCA               TGG^CCA
MslI          948  CATCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        109  CAGCTG               C[AC]G^C[GT]G
MspI          968  CCGG                 C^CGG
MvnI          521  CGCG                 CG^CG
NciI          968  CCGGG                CC^[GC]GG
NcoI          828  CCATGG               C^CATGG
NdeI          372  CATATG               CA^TATG
PaeR7I        171  CTCGAG               C^TCGAG
PflFI         265  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         109  CAGCTG               CAG^CTG
RcaI          305  TCATGA               T^CATGA
SbfI         1173  CCTGCAGG             CCTGCA^GG
SexAI         611  ACCTGGT              A^CC[AT]GGT
Sse8387I     1173  CCTGCAGG             CCTGCA^GG
TfiI          936  GATTC                G^A[AT]TC
Tth111I       265  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          171  CTCGAG               C^TCGAG
XhoII         479  AGATCT               [GA]^GATC[CT]
XmnI         1095  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AvaII         117  GGTCC                G^G[AT]CC
              902  GGACC               
BsaAI         700  TACGTG               [CT]AC^GT[GA]
              796  CACGTA              
BseRI          19  GAGGAG               GAGGAG(10/8)
              619  GAGGAG              
BsmFI         287  GGGAC                GGGAC(10/14)
              901  GGGAC               
BspMI         646  ACCTGC               ACCTGC(4/8)
             1049  ACCTGC              
Cac8I          24  GCTGGC               GC[ACGT]^[ACGT]GC
             1172  GCCTGC              
DpnII         105  GATC                 ^GATC
              480  GATC                
DsaI          497  CCGTGG               C^C[GA][CT]GG
              828  CCATGG              
EcoNI         612  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              918  CCTCTTCAAGG         
EcoT14I       423  CCAAGG               C^C[AT][AT]GG
              828  CCATGG              
FauI           56  CCCGC                CCCGC(4/6)
              519  CCCGC               
MboI          105  GATC                 ^GATC
              480  GATC                
NdeII         105  GATC                 ^GATC
              480  GATC                
PflMI         108  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              767  CCATCGACTGG         
PleI          336  GACTC                GAGTC(4/5)
              712  GAGTC               
PstI          807  CTGCAG               CTGCA^G
             1174  CTGCAG              
Sau3AI        105  GATC                 ^GATC
              480  GATC                
SfcI          807  CTGCAG               C^T[GA][CT]AG
             1174  CTGCAG              
StyI          423  CCAAGG               C^C[AT][AT]GG
              828  CCATGG              
Tsp509I       313  AATT                 ^AATT
             1014  AATT                
Van91I        108  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              767  CCATCGACTGG         
XcmI          881  CCAGGCACTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1157  CCAGCTCCACCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        AluI        BbsI        BbvI        
BmyI        BpuAI       BsaJI       BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
CfoI        Cfr13I      DdeI        EaeI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboII       MnlI        MvaI        
MwoI        NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       SfaNI       SmlI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     
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