Restriction map for pF1KE4378
 (Linear) (Six-base) MAPSORT of: KIEE4378.seq  Check: 317  from: 1  to: 1197



KIEE4378.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AccI GT'mk_AC

Cuts at:      0     125    1197 
   Size:        125    1072
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     711     855    1197 
   Size:        711     144     342

  Fragments arranged by size: 

                 711     342     144
 
AflIII A'CryG_T

Cuts at:      0     472    1197 
   Size:        472     725
 
AlwNI CAG_nnn'CTG

Cuts at:      0     392    1197 
   Size:        392     805
 
AvaI C'yCGr_G

Cuts at:      0     171    1197 
   Size:        171    1026
 
BanI G'GyrC_C

Cuts at:      0     491    1197 
   Size:        491     706
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     264     389     475     649     678    1197 
   Size:        264     125      86     174      29     519

  Fragments arranged by size: 

                 519     264     174     125      86      29
 
BbvCI CC'TCA_GC

Cuts at:      0    1112    1197 
   Size:       1112      85
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     750     784    1197 
   Size:        750      34     413

  Fragments arranged by size: 

                 750     413      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1077    1197 
   Size:       1077     120
 
BglII A'GATC_T

Cuts at:      0     479    1197 
   Size:        479     718
 
BlpI GC'TnA_GC

Cuts at:      0    1136    1197 
   Size:       1136      61
 
Bme1580I G_kGCm'C

Cuts at:      0     496     682    1197 
   Size:        496     186     515

  Fragments arranged by size: 

                 515     496     186
 
BmgBI CAC'GTC

Cuts at:      0     473    1197 
   Size:        473     724
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     676    1197 
   Size:        676     521
 
Bpu10I CC'TnA_GC

Cuts at:      0    1112    1197 
   Size:       1112      85
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     323    1197 
   Size:        190     133     874

  Fragments arranged by size: 

                 874     190     133
 
BsaAI yAC'GTr

Cuts at:      0     702     798    1197 
   Size:        702      96     399

  Fragments arranged by size: 

                 702     399      96
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     634    1197 
   Size:         34     600     563

  Fragments arranged by size: 

                 600     563      34
 
BseYI C'CCAG_C

Cuts at:      0     224     276    1197 
   Size:        224      52     921

  Fragments arranged by size: 

                 921     224      52
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652    1197 
   Size:        652     545
 
BsiHKAI G_wGCw'C

Cuts at:      0     359    1197 
   Size:        359     838
 
BsiWI C'GTAC_G

Cuts at:      0     798    1197 
   Size:        798     399
 
BsmI GAATG_Cn'

Cuts at:      0     633    1197 
   Size:        633     564
 
Bsp1286I G_dGCh'C

Cuts at:      0     359     496     682    1197 
   Size:        359     137     186     515

  Fragments arranged by size: 

                 515     359     186     137
 
BspHI T'CATG_A

Cuts at:      0     305    1197 
   Size:        305     892
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     655    1058    1197 
   Size:        655     403     139

  Fragments arranged by size: 

                 655     403     139
 
BsrBI CCG'CTC

Cuts at:      0     176    1197 
   Size:        176    1021
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     232    1197 
   Size:        232     965
 
BstYI r'GATC_y

Cuts at:      0     479    1197 
   Size:        479     718
 
BtgI C'CryG_G

Cuts at:      0     497     828    1197 
   Size:        497     331     369

  Fragments arranged by size: 

                 497     369     331
 
BtsI GCAGTG_nn'

Cuts at:      0     195     804    1197 
   Size:        195     609     393

  Fragments arranged by size: 

                 609     393     195
 
DraIII CAC_nnn'GTG

Cuts at:      0     891    1197 
   Size:        891     306
 
EaeI y'GGCC_r

Cuts at:      0      26     215    1031    1197 
   Size:         26     189     816     166

  Fragments arranged by size: 

                 816     189     166      26
 
EarI CTCTTCn'nnn_

Cuts at:      0      43     836     925    1197 
   Size:         43     793      89     272

  Fragments arranged by size: 

                 793     272      89      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     711     855    1197 
   Size:        711     144     342

  Fragments arranged by size: 

                 711     342     144
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     616     922    1197 
   Size:        616     306     275

  Fragments arranged by size: 

                 616     306     275
 
EcoO109I rG'GnC_Cy

Cuts at:      0     210    1197 
   Size:        210     987
 
FspI TGC'GCA

Cuts at:      0     351    1197 
   Size:        351     846
 
HincII GTy'rAC

Cuts at:      0     600    1197 
   Size:        600     597
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     123    1197 
   Size:        123    1074
 
MscI TGG'CCA

Cuts at:      0    1033    1197 
   Size:       1033     164
 
MslI CAynn'nnrTG

Cuts at:      0     952    1197 
   Size:        952     245
 
MspA1I CmG'CkG

Cuts at:      0     111    1197 
   Size:        111    1086
 
NcoI C'CATG_G

Cuts at:      0     828    1197 
   Size:        828     369
 
NdeI CA'TA_TG

Cuts at:      0     373    1197 
   Size:        373     824
 
PasI CC'CwG_GG

Cuts at:      0     282     400    1167    1197 
   Size:        282     118     767      30

  Fragments arranged by size: 

                 767     282     118      30
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     114     773    1197 
   Size:        114     659     424

  Fragments arranged by size: 

                 659     424     114
 
PfoI T'CCnGG_A

Cuts at:      0      14    1197 
   Size:         14    1183
 
PspXI vC'TCGA_Gb

Cuts at:      0     171    1197 
   Size:        171    1026
 
PstI C_TGCA'G

Cuts at:      0     811    1178    1197 
   Size:        811     367      19

  Fragments arranged by size: 

                 811     367      19
 
PvuII CAG'CTG

Cuts at:      0     111    1197 
   Size:        111    1086
 
SbfI CC_TGCA'GG

Cuts at:      0    1178    1197 
   Size:       1178      19
 
SexAI A'CCwGG_T

Cuts at:      0     611    1197 
   Size:        611     586
 
SfcI C'TryA_G

Cuts at:      0     807    1174    1197 
   Size:        807     367      23

  Fragments arranged by size: 

                 807     367      23
 
SmlI C'TyrA_G

Cuts at:      0     171     205     338    1197 
   Size:        171      34     133     859

  Fragments arranged by size: 

                 859     171     133      34
 
StyI C'CwwG_G

Cuts at:      0     423     828    1197 
   Size:        423     405     369

  Fragments arranged by size: 

                 423     405     369
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     129     374    1197 
   Size:        129     245     823

  Fragments arranged by size: 

                 823     245     129
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      28    1197 
   Size:         28    1169
 
Tth111I GACn'n_nGTC

Cuts at:      0     268    1197 
   Size:        268     929
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     888    1164    1197 
   Size:        888     276      33

  Fragments arranged by size: 

                 888     276      33
 
XhoI C'TCGA_G

Cuts at:      0     171    1197 
   Size:        171    1026
 
XmnI GAAnn'nnTTC

Cuts at:      0    1099    1197 
   Size:       1099      98
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     AvaI     BanI     BbsI    BbvCI
     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI   Bpu10I
    BpuEI    BsaAI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspHI    BspMI    BsrBI    BstXI    BstYI     BtgI     BtsI
   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FspI   HincII
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     PasI    PflMI
     PfoI    PspXI     PstI    PvuII     SbfI    SexAI     SfcI     SmlI
     StyI    TaqII     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BciVI     BclI
    BfrBI     BmtI     BplI     BpmI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI    BsmBI    BspEI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     XbaI     XmaI     ZraI
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