Restriction map for pF1KE1913
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp718I     
AvrII       BbeI        BbrPI       BbvCI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        HindIII     HpaI        KasI        KpnI        
MaeI        MamI        MfeI        MluI        MroI        
MseI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SapI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        Tru9I       XbaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          145  GTCTAC               GT^[AC][GT]AC
AcyI         1279  GACGCC               G[GA]^CG[CT]C
AhdI         1249  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I         13  GTGCAC               G^TGCAC
ApaLI          13  GTGCAC               G^TGCAC
Asp700I       492  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1249  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          220  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         749  TGCGCA               TGC^GCA
BamHI        1031  GGATCC               G^GATCC
BanI          771  GGTGCC               G^G[CT][GA]CC
BbsI         1312  GAAGAC               GAAGAC(2/6)
BciVI         474  GTATCC               GTATCC(6/5)
BpmI          130  CTGGAG               CTGGAG(16/14)
BpuAI        1312  GAAGAC               GAAGAC(2/6)
BsaHI        1279  GACGCC               G[GA]^CG[CT]C
BsaI          592  GAGACC               GGTCTC(1/5)
BsmAI         592  GAGAC                GTCTC(1/5)
BsmFI         668  GGGAC                GGGAC(10/14)
BspHI         488  TCATGA               T^CATGA
BspMI         746  ACCTGC               ACCTGC(4/8)
BstXI         188  CCATTCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1031  GGATCC               [GA]^GATC[CT]
DdeI          231  CTCAG                C^T[ACGT]AG
Eam1105I     1249  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI           24  GGCCGGCC             GGCCGG^CC
FspI          749  TGCGCA               TGC^GCA
HaeII         615  GGCGCT               [GA]GCGC^[CT]
Hin1I        1279  GACGCC               G[GA]^CG[CT]C
HincII        763  GTCAAC               GT[CT]^[GA]AC
HindII        763  GTCAAC               GT[CT]^[GA]AC
KspI         1074  CCGCGG               CCGC^GG
MflI         1031  GGATCC               [GA]^GATC[CT]
NcoI          215  CCATGG               C^CATGG
NspI          959  GCATGT               [GA]CATG^[CT]
PaeR7I        182  CTCGAG               C^TCGAG
PflFI         220  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         668  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          388  CTGCAG               CTGCA^G
RcaI          488  TCATGA               T^CATGA
SacI          862  GAGCTC               GAGCT^C
SacII        1074  CCGCGG               CCGC^GG
SbfI          387  CCTGCAGG             CCTGCA^GG
SfcI          388  CTGCAG               C^T[GA][CT]AG
Sse8387I      387  CCTGCAGG             CCTGCA^GG
TfiI          340  GATTC                G^A[AT]TC
Tsp509I       265  AATT                 ^AATT
Tth111I       220  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          182  CTCGAG               C^TCGAG
XhoII        1031  GGATCC               [GA]^GATC[CT]
XmnI          492  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwNI         985  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1123  CAGGAGCTG           
ApaI          845  GGGCCC               GGGCC^C
             1341  GGGCCC              
AspHI          13  GTGCAC               G[AT]GC[AT]^C
              862  GAGCTC              
BcgI           38  GCAACCAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1013  GCAGCTACTTCG        
BglI           29  GCCAGTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              386  GCCTGCAGGGC         
BsaAI         106  TACGTG               [CT]AC^GT[GA]
              175  TACGTG              
BsgI          787  CTGCAC               GTGCAG(16/14)
             1183  CTGCAC              
BsiHKAI        13  GTGCAC               G[AT]GC[AT]^C
              862  GAGCTC              
BsrFI          25  GCCGGC               [GA]^CCGG[CT]
              289  GCCGGC              
Cfr10I         25  GCCGGC               [GA]^CCGG[CT]
              289  GCCGGC              
EagI          921  CGGCCG               C^GGCCG
             1286  CGGCCG              
EclXI         921  CGGCCG               C^GGCCG
             1286  CGGCCG              
Eco52I        921  CGGCCG               C^GGCCG
             1286  CGGCCG              
NaeI           25  GCCGGC               GCC^GGC
              289  GCCGGC              
NgoMIV         25  GCCGGC               G^CCGGC
              289  GCCGGC              
SexAI         680  ACCTGGT              A^CC[AT]GGT
             1250  ACCTGGT             
SmaI          726  CCCGGG               CCC^GGG
              824  CCCGGG              
XmaCI         726  CCCGGG               C^CCGGG
              824  CCCGGG              
XmaI          726  CCCGGG               C^CCGGG
              824  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaI        AvaII       BalI        BanII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiEI       
BsiYI       BslI        BsoBI       Bsp1286I    BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StuI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        
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