Restriction map for pF1KE2340
 (Circular) (Six-base) MAPSORT of: pF1KE2340.seq  Check: 3343  from: 1  to: 3623



pF1KE2340  3623 bp

 With 182 enzymes: SgfI * 

                        March 29, 2011 17:34  ..

AatII G_ACGT'C

Cuts at:    404     404 
   Size:       3623
 
AccI GT'mk_AC

Cuts at:    529     661     529 
   Size:        132    3491
 
Acc65I G'GTAC_C

Cuts at:    639     639 
   Size:       3623
 
AclI AA'CG_TT

Cuts at:   3463    3463 
   Size:       3623
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    165     578    1448    1880    2627    2895     165 
   Size:        413     870     432     747     268     893

  Fragments arranged by size: 

                 893     870     747     432     413     268
 
AflIII A'CryG_T

Cuts at:    975    2079    3056     975 
   Size:       1104     977    1542

  Fragments arranged by size: 

                1542    1104     977
 
AgeI A'CCGG_T

Cuts at:   2011    2011 
   Size:       3623
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    450     450 
   Size:       3623
 
AlwNI CAG_nnn'CTG

Cuts at:    928    2495     928 
   Size:       1567    2056
 
ApaLI G'TGCA_C

Cuts at:   2393    2393 
   Size:       3623
 
ApoI r'AATT_y

Cuts at:    627    2976    3232     627 
   Size:       2349     256    1018

  Fragments arranged by size: 

                2349    1018     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3623
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3623
 
AvaI C'yCGr_G

Cuts at:    199     643     199 
   Size:        444    3179
 
BamHI G'GATC_C

Cuts at:    648     648 
   Size:       3623
 
BanI G'GyrC_C

Cuts at:    639    1053    1301    1336     639 
   Size:        414     248      35    2926

  Fragments arranged by size: 

                2926     414     248      35
 
BanII G_rGCy'C

Cuts at:    194     637    1667     194 
   Size:        443    1030    2150

  Fragments arranged by size: 

                2150    1030     443
 
BbeI G_GCGC'C

Cuts at:   1057    1305    1057 
   Size:        248    3375
 
BbsI GAAGACnn'nnnn_

Cuts at:    566     566 
   Size:       3623
 
BciVI GTATCCnnnnn_n'

Cuts at:   1521    2282    3623    1521 
   Size:        761    1341    1521

  Fragments arranged by size: 

                1521    1341     761
 
BclI T'GATC_A

Cuts at:   1143    1143 
   Size:       3623
 
BglI GCCn_nnn'nGGC

Cuts at:    353     505     353 
   Size:        152    3471
 
BglII A'GATC_T

Cuts at:   1138    3175    1138 
   Size:       2037    1586
 
BlpI GC'TnA_GC

Cuts at:    735     735 
   Size:       3623
 
Bme1580I G_kGCm'C

Cuts at:   1248    1341    2397    1248 
   Size:         93    1056    2474

  Fragments arranged by size: 

                2474    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    255     835     916    1251    3395     255 
   Size:        580      81     335    2144     483

  Fragments arranged by size: 

                2144     580     483     335      81
 
BmtI G_CTAG'C

Cuts at:    457     457 
   Size:       3623
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1990    1990 
   Size:       3623
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    799    1678    2170    2468     799 
   Size:        879     492     298    1954

  Fragments arranged by size: 

                1954     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3327    3327 
   Size:       3623
 
BsaAI yAC'GTr

Cuts at:    976     976 
   Size:       3623
 
BsaBI GATnn'nnATC

Cuts at:      6    1137    3174       6 
   Size:       1131    2037     455

  Fragments arranged by size: 

                2037    1131     455
 
BsaHI Gr'CG_yC

Cuts at:    401    1054    1302    2004    3500     401 
   Size:        653     248     702    1496     524

  Fragments arranged by size: 

                1496     702     653     524     248
 
BsaWI w'CCGG_w

Cuts at:    812    1333    2011    2285    2432    2843     812 
   Size:        521     678     274     147     411    1592

  Fragments arranged by size: 

                1592     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    147     177     147 
   Size:         30    3593
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    265     403     265 
   Size:        138    3485
 
BseYI C'CCAG_C

Cuts at:    495    1048    1806    2383    2879     495 
   Size:        553     758     577     496    1239

  Fragments arranged by size: 

                1239     758     577     553     496
 
BsiEI CG_ry'CG

Cuts at:    104     171    1165    1211    2419     104 
   Size:         67     994      46    1208    1308

  Fragments arranged by size: 

                1308    1208     994      67      46
 
BsiHKAI G_wGCw'C

Cuts at:    464     637    1415    1605    2397     464 
   Size:        173     778     190     792    1690

  Fragments arranged by size: 

                1690     792     778     190     173
 
Bsp1286I G_dGCh'C

Cuts at:    194     464     637    1248    1341    1415    1605    1667    2397 
   Size:        270     173     611      93      74     190      62     730

Cuts at:   2397     194 
   Size:       1420

  Fragments arranged by size: 

                1420     730     611     270     190     173      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2843    2843 
   Size:       3623
 
BspHI T'CATG_A

Cuts at:   2799    2799 
   Size:       3623
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    673     887    1189    1570     673 
   Size:        214     302     381    2726

  Fragments arranged by size: 

                2726     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1915    1969    3453    3618      50 
   Size:       1865      54    1484     165      55

  Fragments arranged by size: 

                1865    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1535    1535 
   Size:       3623
 
BsrFI r'CCGG_y

Cuts at:    118     170     482    1621    1802    2011     118 
   Size:         52     312    1139     181     209    1730

  Fragments arranged by size: 

                1730    1139     312     209     181      52
 
BssSI C'ACGA_G

Cuts at:   1894    2252    1894 
   Size:        358    3265
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1000    1000 
   Size:       3623
 
BstBI TT'CG_AA

Cuts at:   1984    1984 
   Size:       3623
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     114 
   Size:       3623
 
BstYI r'GATC_y

Cuts at:      1     444     648    1115    1138    1473    1719    2731    2817 
   Size:        443     204     467      23     335     246    1012      86

Cuts at:   2817    2829    3175       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     443     346     335     246     204
                  86      23      12
 
BstZ17I GTA'TAC

Cuts at:    530     530 
   Size:       3623
 
BtgI C'CryG_G

Cuts at:    543     543 
   Size:       3623
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1573    1754    1917    1573 
   Size:        181     163    3279

  Fragments arranged by size: 

                3279     181     163
 
BtsI GCAGTG_nn'

Cuts at:    155     464     866     155 
   Size:        309     402    2912

  Fragments arranged by size: 

                2912     402     309
 
DraI TTT'AAA

Cuts at:    622     622 
   Size:       3623
 
DrdI GACnn_nn'nnGTC

Cuts at:   1329    2187    1329 
   Size:        858    2765
 
EaeI y'GGCC_r

Cuts at:    116     168    1208    1382    1773    1800    3145     116 
   Size:         52    1040     174     391      27    1345     594

  Fragments arranged by size: 

                1345    1040     594     391     174      52      27
 
EagI C'GGCC_G

Cuts at:    168    1208     168 
   Size:       1040    2583
 
EarI CTCTTCn'nnn_

Cuts at:    522    1646    1856     522 
   Size:       1124     210    2289

  Fragments arranged by size: 

                2289    1124     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    199    1831    2141    2287    3433     199 
   Size:       1632     310     146    1146     389

  Fragments arranged by size: 

                1632    1146     389     310     146
 
EcoICRI GAG'CTC

Cuts at:    635     635 
   Size:       3623
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    165     578    1448    1880    2627    2895     165 
   Size:        413     870     432     747     268     893

  Fragments arranged by size: 

                 893     870     747     432     413     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    385     385 
   Size:       3623
 
EcoO109I rG'GnC_Cy

Cuts at:    574     762     574 
   Size:        188    3435
 
EcoRI G'AATT_C

Cuts at:    627     627 
   Size:       3623
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    409    1642     409 
   Size:       1233    2390
 
FspI TGC'GCA

Cuts at:   1404    1404 
   Size:       3623
 
HaeII r_GCGC'y

Cuts at:   1057    1305    2327    3306    1057 
   Size:        248    1022     979    1374

  Fragments arranged by size: 

                1374    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1465    1495    1497    1527    1465 
   Size:         30       2      30    3561

  Fragments arranged by size: 

                3561      30      30       2
 
HincII GTy'rAC

Cuts at:    662     662 
   Size:       3623
 
KasI G'GCGC_C

Cuts at:   1053    1301    1053 
   Size:        248    3375
 
KpnI G_GTAC'C

Cuts at:    643     643 
   Size:       3623
 
MluI A'CGCG_T

Cuts at:   3056    3056 
   Size:       3623
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2294    2478    2294 
   Size:        184    3439
 
MscI TGG'CCA

Cuts at:   1384    1384 
   Size:       3623
 
MslI CAynn'nnrTG

Cuts at:    112    1612    1739     112 
   Size:       1500     127    1996

  Fragments arranged by size: 

                1996    1500     127
 
MspA1I CmG'CkG

Cuts at:    434     734    1048    1408    2421    2666     434 
   Size:        300     314     360    1013     245    1391

  Fragments arranged by size: 

                1391    1013     360     314     300     245
 
NaeI GCC'GGC

Cuts at:   1804    1804 
   Size:       3623
 
NarI GG'CG_CC

Cuts at:   1054    1302    1054 
   Size:        248    3375
 
NcoI C'CATG_G

Cuts at:    543     543 
   Size:       3623
 
NgoMIV G'CCGG_C

Cuts at:   1802    1802 
   Size:       3623
 
NheI G'CTAG_C

Cuts at:    453     453 
   Size:       3623
 
NspI r_CATG'y

Cuts at:    676    1176    2083     676 
   Size:        500     907    2216

  Fragments arranged by size: 

                2216     907     500
 
PasI CC'CwG_GG

Cuts at:    365     365 
   Size:       3623
 
PciI A'CATG_T

Cuts at:   2079    2079 
   Size:       3623
 
PflMI CCAn_nnn'nTGG

Cuts at:    570     570 
   Size:       3623
 
PmeI GTTT'AAAC

Cuts at:    622     622 
   Size:       3623
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2762    2794    2762 
   Size:         32    3591
 
PspXI vC'TCGA_Gb

Cuts at:    199     199 
   Size:       3623
 
PstI C_TGCA'G

Cuts at:    670    1355     670 
   Size:        685    2938
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3623
 
PvuII CAG'CTG

Cuts at:   1048    1408    1048 
   Size:        360    3263
 
RsrII CG'GwC_CG

Cuts at:   1818    1818 
   Size:       3623
 
SacI G_AGCT'C

Cuts at:    637     637 
   Size:       3623
 
SalI G'TCGA_C

Cuts at:    660     660 
   Size:       3623
 
SapI GCTCTTCn'nnn_

Cuts at:    522    1646    1856     522 
   Size:       1124     210    2289

  Fragments arranged by size: 

                2289    1124     210
 
SbfI CC_TGCA'GG

Cuts at:    670     670 
   Size:       3623
 
ScaI AGT'ACT

Cuts at:   3140    3140 
   Size:       3623
 
SexAI A'CCwGG_T

Cuts at:    413     413 
   Size:       3623
 
SfcI C'TryA_G

Cuts at:     33     666    1351    2344    2535      33 
   Size:        633     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     633     191
 
SfoI GGC'GCC

Cuts at:   1055    1303    1055 
   Size:        248    3375
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3623
 
SmaI CCC'GGG

Cuts at:    645     645 
   Size:       3623
 
SmlI C'TyrA_G

Cuts at:    199     778    1693    2185    2447     199 
   Size:        579     915     492     262    1375

  Fragments arranged by size: 

                1375     915     579     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3623
 
SphI G_CATG'C

Cuts at:    676    1176     676 
   Size:        500    3123
 
StyI C'CwwG_G

Cuts at:    284     351     543     757    1111     284 
   Size:         67     192     214     354    2796

  Fragments arranged by size: 

                2796     354     214     192      67
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1206    1339    1798    2007    1206 
   Size:        133     459     209    2822

  Fragments arranged by size: 

                2822     459     209     133
 
TatI w'GTAC_w

Cuts at:   3138    3138 
   Size:       3623
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    147     179    3140    3172     147 
   Size:         32    2961      32     598

  Fragments arranged by size: 

                2961     598      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1420    1420 
   Size:       3623
 
XbaI T'CTAG_A

Cuts at:    654     654 
   Size:       3623
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1118    1118 
   Size:       3623
 
XhoI C'TCGA_G

Cuts at:    199     199 
   Size:       3623
 
XmaI C'CCGG_G

Cuts at:    643     643 
   Size:       3623
 
XmnI GAAnn'nnTTC

Cuts at:    317     317 
   Size:       3623
 
ZraI GAC'GTC

Cuts at:    402     402 
   Size:       3623
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BpmI   Bpu10I
     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII   Bsu36I
     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NotI     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI    PshAI     PsiI   PspOMI     PsrI    SacII    SanDI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com