################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 451 GTCGAC GT^[AC][GT]AC AcyI 490 GACGCC G[GA]^CG[CT]C AflIII 413 ACATGT A^C[GA][CT]GT AlwI 78 GATCC GGATC(4/5) Asp700I 348 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BclI 296 TGATCA T^GATCA BmyI 336 GGGCAC G[AGT]GC[ACT]^C BsaHI 490 GACGCC G[GA]^CG[CT]C BsaWI 301 ACCGGA [AT]^CCGG[AT] Bsp1286I 336 GGGCAC G[AGT]GC[ACT]^C BspLU11I 413 ACATGT A^CATGT BstYI 77 AGATCC [GA]^GATC[CT] Cac8I 220 GCCAGC GC[ACGT]^[ACGT]GC DraII 143 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 375 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 167 CCATGG C^C[GA][CT]GG EcoO109I 143 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 167 CCATGG C^C[AT][AT]GG FbaI 296 TGATCA T^GATCA HapII 302 CCGG C^CGG Hin1I 490 GACGCC G[GA]^CG[CT]C HincII 451 GTCGAC GT[CT]^[GA]AC HindII 451 GTCGAC GT[CT]^[GA]AC HinfI 72 GACTC G^A[ACGT]TC HpaII 302 CCGG C^CGG MaeIII 383 GTGAC ^GT[ACGT]AC MflI 77 AGATCC [GA]^GATC[CT] MspA1I 222 CAGCGG C[AC]G^C[GT]G MspI 302 CCGG C^CGG MwoI 369 GCTGACCACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 167 CCATGG C^CATGG NspI 413 ACATGT [GA]CATG^[CT] PleI 72 GACTC GAGTC(4/5) PpuMI 143 AGGACCT [GA]G^G[AT]CC[CT] SalI 451 GTCGAC G^TCGAC SfaNI 202 GATGC GCATC(5/9) SmlI 271 CTCAAG C^T[CT][GA]AG StuI 95 AGGCCT AGG^CCT StyI 167 CCATGG C^C[AT][AT]GG Tsp45I 383 GTGAC ^GT[GC]AC XcmI 114 CCAGAACCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 77 AGATCC [GA]^GATC[CT] XmnI 348 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AocI 286 CCTGAGG CC^T[ACGT]AGG 325 CCTGAGG BbsI 52 GTCTTC GAAGAC(2/6) 244 GTCTTC BbvI 163 GCAGC GCAGC(8/12) 366 GCTGC BpmI 45 CTCCAG CTGGAG(16/14) 358 CTGGAG BpuAI 52 GTCTTC GAAGAC(2/6) 244 GTCTTC BsmFI 152 GGGAC GGGAC(10/14) 227 GTCCC BstF5I 201 GGATG GGATG(2/0) 291 GGATG Bsu36I 286 CCTGAGG CC^T[ACGT]AGG 325 CCTGAGG Eco81I 286 CCTGAGG CC^T[ACGT]AGG 325 CCTGAGG FokI 201 GGATG GGATG(9/13) 291 GGATG HgaI 490 GACGC GACGC(5/10) 50 GCGTC HphI 239 TCACC GGTGA(8/7) 299 TCACC TseI 163 GCAGC G^C[AT]GC 366 GCTGC TspRI 102 CACTG CA[GC]TG[ACGT][ACGT]^ 379 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI AvaII BanI BsaJI BseRI BsiYI BslI BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII ItaI MaeII MboI MboII MnlI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TaqI TthHB8I