(Circular) (Six-base) MAPSORT of: pF1KE2576.seq Check: 447 from: 1 to: 3620 pF1KE2576 3620 bp With 182 enzymes: SgfI * May 26, 2015 10:14 .. AccI GT'mk_AC Cuts at: 560 658 560 Size: 98 3522 Acc65I G'GTAC_C Cuts at: 636 636 Size: 3620 AclI AA'CG_TT Cuts at: 3460 3460 Size: 3620 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1445 1877 2624 2892 1445 Size: 432 747 268 2173 Fragments arranged by size: 2173 747 432 268 AflIII A'CryG_T Cuts at: 521 972 2076 3053 521 Size: 451 1104 977 1088 Fragments arranged by size: 1104 1088 977 451 AgeI A'CCGG_T Cuts at: 2008 2008 Size: 3620 AlwNI CAG_nnn'CTG Cuts at: 925 2492 925 Size: 1567 2053 ApaLI G'TGCA_C Cuts at: 2390 2390 Size: 3620 ApoI r'AATT_y Cuts at: 624 2973 3229 624 Size: 2349 256 1015 Fragments arranged by size: 2349 1015 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3620 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3620 AvaI C'yCGr_G Cuts at: 640 640 Size: 3620 BamHI G'GATC_C Cuts at: 645 645 Size: 3620 BanI G'GyrC_C Cuts at: 111 385 445 636 1050 1298 1333 111 Size: 274 60 191 414 248 35 2398 Fragments arranged by size: 2398 414 274 248 191 60 35 BanII G_rGCy'C Cuts at: 634 1664 634 Size: 1030 2590 BbeI G_GCGC'C Cuts at: 1054 1302 1054 Size: 248 3372 BbsI GAAGACnn'nnnn_ Cuts at: 153 345 153 Size: 192 3428 BciVI GTATCCnnnnn_n' Cuts at: 1518 2279 3620 1518 Size: 761 1341 1518 Fragments arranged by size: 1518 1341 761 BclI T'GATC_A Cuts at: 404 1140 404 Size: 736 2884 BglII A'GATC_T Cuts at: 1135 3172 1135 Size: 2037 1583 BlpI GC'TnA_GC Cuts at: 732 732 Size: 3620 Bme1580I G_kGCm'C Cuts at: 448 1245 1338 2394 448 Size: 797 93 1056 1674 Fragments arranged by size: 1674 1056 797 93 BmgBI CAC'GTC Cuts at: 546 546 Size: 3620 BmrI ACTGGGnnnn_n' Cuts at: 832 913 1248 3392 832 Size: 81 335 2144 1060 Fragments arranged by size: 2144 1060 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1987 1987 Size: 3620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 138 487 138 Size: 349 3271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 364 796 1675 2167 2465 364 Size: 432 879 492 298 1519 Fragments arranged by size: 1519 879 492 432 298 BsaI GGTCTCn'nnnn_ Cuts at: 3324 3324 Size: 3620 BsaAI yAC'GTr Cuts at: 973 973 Size: 3620 BsaBI GATnn'nnATC Cuts at: 6 1134 3171 6 Size: 1128 2037 455 Fragments arranged by size: 2037 1128 455 BsaHI Gr'CG_yC Cuts at: 599 1051 1299 2001 3497 599 Size: 452 248 702 1496 722 Fragments arranged by size: 1496 722 702 452 248 BsaWI w'CCGG_w Cuts at: 409 809 1330 2008 2282 2429 2840 409 Size: 400 521 678 274 147 411 1189 Fragments arranged by size: 1189 678 521 411 400 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 484 514 484 Size: 30 3590 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 316 484 523 316 Size: 168 39 3413 Fragments arranged by size: 3413 168 39 BseYI C'CCAG_C Cuts at: 1045 1803 2380 2876 1045 Size: 758 577 496 1789 Fragments arranged by size: 1789 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1162 1208 2416 104 Size: 1058 46 1208 1308 Fragments arranged by size: 1308 1208 1058 46 BsiHKAI G_wGCw'C Cuts at: 634 1412 1602 2394 634 Size: 778 190 792 1860 Fragments arranged by size: 1860 792 778 190 Bsp1286I G_dGCh'C Cuts at: 448 634 1245 1338 1412 1602 1664 2394 448 Size: 186 611 93 74 190 62 730 1674 Fragments arranged by size: 1674 730 611 190 186 93 74 62 BspEI T'CCGG_A Cuts at: 2840 2840 Size: 3620 BspHI T'CATG_A Cuts at: 2796 2796 Size: 3620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 670 884 1186 1567 670 Size: 214 302 381 2723 Fragments arranged by size: 2723 381 302 214 BsrBI CCG'CTC Cuts at: 50 1912 1966 3450 3615 50 Size: 1862 54 1484 165 55 Fragments arranged by size: 1862 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1532 1532 Size: 3620 BsrFI r'CCGG_y Cuts at: 1618 1799 2008 1618 Size: 181 209 3230 Fragments arranged by size: 3230 209 181 BssSI C'ACGA_G Cuts at: 1891 2249 1891 Size: 358 3262 BstAPI GCAn_nnn'nTGC Cuts at: 997 997 Size: 3620 BstBI TT'CG_AA Cuts at: 1981 1981 Size: 3620 BstYI r'GATC_y Cuts at: 1 185 645 1112 1135 1470 1716 2728 2814 Size: 184 460 467 23 335 246 1012 86 Cuts at: 2814 2826 3172 1 Size: 12 346 449 Fragments arranged by size: 1012 467 460 449 346 335 246 184 86 23 12 Bsu36I CC'TnA_GG Cuts at: 395 434 395 Size: 39 3581 BtgI C'CryG_G Cuts at: 107 275 107 Size: 168 3452 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1570 1751 1914 1570 Size: 181 163 3276 Fragments arranged by size: 3276 181 163 BtsI GCAGTG_nn' Cuts at: 493 863 493 Size: 370 3250 DraI TTT'AAA Cuts at: 619 619 Size: 3620 DraIII CAC_nnn'GTG Cuts at: 488 488 Size: 3620 DrdI GACnn_nn'nnGTC Cuts at: 1326 2184 1326 Size: 858 2762 EaeI y'GGCC_r Cuts at: 1205 1379 1770 1797 3142 1205 Size: 174 391 27 1345 1683 Fragments arranged by size: 1683 1345 391 174 27 EagI C'GGCC_G Cuts at: 1205 1205 Size: 3620 EarI CTCTTCn'nnn_ Cuts at: 1643 1853 1643 Size: 210 3410 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 161 1828 2138 2284 3430 161 Size: 1667 310 146 1146 351 Fragments arranged by size: 1667 1146 351 310 146 EcoICRI GAG'CTC Cuts at: 632 632 Size: 3620 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 138 487 1445 1877 2624 2892 138 Size: 349 958 432 747 268 866 Fragments arranged by size: 958 866 747 432 349 268 EcoO109I rG'GnC_Cy Cuts at: 252 759 252 Size: 507 3113 EcoRI G'AATT_C Cuts at: 624 624 Size: 3620 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1639 1639 Size: 3620 FspI TGC'GCA Cuts at: 1401 1401 Size: 3620 HaeII r_GCGC'y Cuts at: 1054 1302 2324 3303 1054 Size: 248 1022 979 1371 Fragments arranged by size: 1371 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 484 516 1462 1492 1494 1524 484 Size: 32 946 30 2 30 2580 Fragments arranged by size: 2580 946 32 30 30 2 HincII GTy'rAC Cuts at: 561 659 561 Size: 98 3522 KasI G'GCGC_C Cuts at: 1050 1298 1050 Size: 248 3372 KpnI G_GTAC'C Cuts at: 640 640 Size: 3620 MluI A'CGCG_T Cuts at: 3053 3053 Size: 3620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 287 2291 2475 287 Size: 2004 184 1432 Fragments arranged by size: 2004 1432 184 MscI TGG'CCA Cuts at: 1381 1381 Size: 3620 MslI CAynn'nnrTG Cuts at: 1609 1736 1609 Size: 127 3493 MspA1I CmG'CkG Cuts at: 332 731 1045 1405 2418 2663 332 Size: 399 314 360 1013 245 1289 Fragments arranged by size: 1289 1013 399 360 314 245 NaeI GCC'GGC Cuts at: 1801 1801 Size: 3620 NarI GG'CG_CC Cuts at: 1051 1299 1051 Size: 248 3372 NcoI C'CATG_G Cuts at: 107 275 107 Size: 168 3452 NgoMIV G'CCGG_C Cuts at: 1799 1799 Size: 3620 NspI r_CATG'y Cuts at: 525 673 1173 2080 525 Size: 148 500 907 2065 Fragments arranged by size: 2065 907 500 148 PciI A'CATG_T Cuts at: 521 2076 521 Size: 1555 2065 PfoI T'CCnGG_A Cuts at: 188 464 188 Size: 276 3344 PmeI GTTT'AAAC Cuts at: 619 619 Size: 3620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2759 2791 2759 Size: 32 3588 PpuMI rG'GwC_Cy Cuts at: 252 252 Size: 3620 PstI C_TGCA'G Cuts at: 667 1352 667 Size: 685 2935 PvuI CG_AT'CG Cuts at: 104 104 Size: 3620 PvuII CAG'CTG Cuts at: 1045 1405 1045 Size: 360 3260 RsrII CG'GwC_CG Cuts at: 1815 1815 Size: 3620 SacI G_AGCT'C Cuts at: 634 634 Size: 3620 SalI G'TCGA_C Cuts at: 559 657 559 Size: 98 3522 SapI GCTCTTCn'nnn_ Cuts at: 1643 1853 1643 Size: 210 3410 SbfI CC_TGCA'GG Cuts at: 667 667 Size: 3620 ScaI AGT'ACT Cuts at: 3137 3137 Size: 3620 SfcI C'TryA_G Cuts at: 33 663 1348 2341 2532 33 Size: 630 685 993 191 1121 Fragments arranged by size: 1121 993 685 630 191 SfoI GGC'GCC Cuts at: 1052 1300 1052 Size: 248 3372 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3620 SmaI CCC'GGG Cuts at: 642 642 Size: 3620 SmlI C'TyrA_G Cuts at: 379 775 1690 2182 2444 379 Size: 396 915 492 262 1555 Fragments arranged by size: 1555 915 492 396 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3620 SphI G_CATG'C Cuts at: 673 1173 673 Size: 500 3120 StuI AGG'CCT Cuts at: 205 205 Size: 3620 StyI C'CwwG_G Cuts at: 107 275 754 1108 107 Size: 168 479 354 2619 Fragments arranged by size: 2619 479 354 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 129 1203 1336 1795 2004 129 Size: 1074 133 459 209 1745 Fragments arranged by size: 1745 1074 459 209 133 TatI w'GTAC_w Cuts at: 3135 3135 Size: 3620 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3137 3169 3137 Size: 32 3588 Tth111I GACn'n_nGTC Cuts at: 1417 1417 Size: 3620 XbaI T'CTAG_A Cuts at: 651 651 Size: 3620 XcmI CCAnnnn_n'nnnnTGG Cuts at: 229 1115 229 Size: 886 2734 XmaI C'CCGG_G Cuts at: 640 640 Size: 3620 XmnI GAAnn'nnTTC Cuts at: 460 460 Size: 3620 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI