Restriction map for pF1KE0909
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BseAI       
BseRI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DdeI        
DraI        DraIII      Eam1105I    Eco47III    Eco81I      
EcoNI       EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MroI        MseI        
MslI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfcI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          490  GTCTAC               GT^[AC][GT]AC
AcyI          147  GGCGCC               G[GA]^CG[CT]C
AflIII         14  ACGCGT               A^C[GA][CT]GT
AlwNI          34  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI          206  TGGCCA               TGG^CCA
BanI          147  GGCGCC               G^G[CT][GA]CC
BbeI          147  GGCGCC               GGCGC^C
BglII         401  AGATCT               A^GATCT
BpmI           40  CTGGAG               CTGGAG(16/14)
BsaHI         147  GGCGCC               G[GA]^CG[CT]C
BsaI          646  GGTCTC               GGTCTC(1/5)
BsaWI         543  TCCGGT               [AT]^CCGG[AT]
BsiEI         245  CGGCCG               CG[GA][CT]^CG
BsmAI         647  GTCTC                GTCTC(1/5)
BspMI         273  GCAGGT               ACCTGC(4/8)
BsrI          665  ACTGG                ACTGG(1/-1)
BstEII        276  GGTGACC              G^GT[ACGT]ACC
BstF5I        123  GGATG                GGATG(2/0)
BstPI         276  GGTGACC              G^GT[ACGT]ACC
DsaI          248  CCGTGG               C^C[GA][CT]GG
EagI          245  CGGCCG               C^GGCCG
EclXI         245  CGGCCG               C^GGCCG
Eco52I        245  CGGCCG               C^GGCCG
EcoO65I       276  GGTGACC              G^GT[ACGT]ACC
FokI          123  GGATG                GGATG(9/13)
Hin1I         147  GGCGCC               G[GA]^CG[CT]C
KasI          147  GGCGCC               G^GCGCC
MluI           14  ACGCGT               A^CGCGT
MluNI         206  TGGCCA               TGG^CCA
MscI          206  TGGCCA               TGG^CCA
NaeI          102  GCCGGC               GCC^GGC
NarI          147  GGCGCC               GG^CGCC
NgoMIV        102  GCCGGC               G^CCGGC
PpuMI         338  GGGACCT              [GA]G^G[AT]CC[CT]
SapI          159  GAAGAGC              GCTCTTC(1/4)
SexAI         359  ACCAGGT              A^CC[AT]GGT
SfaNI         653  GCATC                GCATC(5/9)
SfoI          147  GGCGCC               GGC^GCC
SmlI          439  CTCAAG               C^T[CT][GA]AG
TspRI         107  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII          15  CGCG                 CG^CG
              475  CGCG                
AfaI          141  GTAC                 GT^AC
              213  GTAC                
AlwI          174  GATCC                GGATC(4/5)
              327  GGATC               
BsgI          448  CTGCAC               GTGCAG(16/14)
              271  GTGCAG              
BsmFI         338  GGGAC                GGGAC(10/14)
              597  GTCCC               
BsrFI         102  GCCGGC               [GA]^CCGG[CT]
              243  ACCGGC              
BstUI          15  CGCG                 CG^CG
              475  CGCG                
Cfr10I        102  GCCGGC               [GA]^CCGG[CT]
              243  ACCGGC              
DrdI          288  GACACTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              481  GACAAGACCGTC        
EaeI          206  TGGCCA               [CT]^GGCC[GA]
              245  CGGCCG              
EarI          601  CTCTTC               CTCTTC(1/4)
              159  GAAGAG              
FauI          219  GCGGG                CCCGC(4/6)
              576  GCGGG               
HgaI           13  GACGC                GACGC(5/10)
              621  GACGC               
HinfI          71  GAGTC                G^A[ACGT]TC
              540  GACTC               
HphI          276  GGTGA                GGTGA(8/7)
              605  TCACC               
Ksp632I       601  CTCTTC               CTCTTC(1/4)
              159  GAAGAG              
MaeIII        277  GTGAC                ^GT[ACGT]AC
              538  GTGAC               
MvnI           15  CGCG                 CG^CG
              475  CGCG                
NlaIII        324  CATG                 CATG^
              495  CATG                
PleI          540  GACTC                GAGTC(4/5)
               71  GAGTC               
PvuII          34  CAGCTG               CAG^CTG
              181  CAGCTG              
RsaI          141  GTAC                 GT^AC
              213  GTAC                
Tsp45I        277  GTGAC                ^GT[GC]AC
              538  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        ApaI        AvaI        AvaII       
BanII       BbvI        BcnI        BmyI        BsaJI       
BsiYI       BslI        BsoBI       Bsp1286I    BssKI       
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DpnII       DraII       EcoO109I    EcoRII      Fnu4HI      
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIV       Sau3AI      Sau96I      
ScrFI       TseI        XhoII       
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