Restriction map for pF1KE0909
 (Linear) (Six-base) MAPSORT of: KIEE0909.seq  Check: 4888  from: 1  to: 675



KIEE0909.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     264     675 
   Size:        264     411
 
AccI GT'mk_AC

Cuts at:      0     491     675 
   Size:        491     184
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     394     675 
   Size:        373      21     281

  Fragments arranged by size: 

                 373     281      21
 
AflIII A'CryG_T

Cuts at:      0      14     675 
   Size:         14     661
 
AlwNI CAG_nnn'CTG

Cuts at:      0      39     675 
   Size:         39     636
 
ApaI G_GGCC'C

Cuts at:      0     236     317     583     675 
   Size:        236      81     266      92

  Fragments arranged by size: 

                 266     236      92      81
 
AvaI C'yCGr_G

Cuts at:      0      68     136     513     675 
   Size:         68      68     377     162

  Fragments arranged by size: 

                 377     162      68      68
 
BanI G'GyrC_C

Cuts at:      0     147     675 
   Size:        147     528
 
BanII G_rGCy'C

Cuts at:      0     236     317     583     675 
   Size:        236      81     266      92

  Fragments arranged by size: 

                 266     236      92      81
 
BbeI G_GCGC'C

Cuts at:      0     151     675 
   Size:        151     524
 
BglII A'GATC_T

Cuts at:      0     401     675 
   Size:        401     274
 
Bme1580I G_kGCm'C

Cuts at:      0     137     236     317     583     675 
   Size:        137      99      81     266      92

  Fragments arranged by size: 

                 266     137      99      92      81
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     675 
   Size:         61     614
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     675 
   Size:        424     251
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     652     675 
   Size:        652      23
 
BsaHI Gr'CG_yC

Cuts at:      0     148     675 
   Size:        148     527
 
BsaWI w'CCGG_w

Cuts at:      0     543     675 
   Size:        543     132
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     517     547     675 
   Size:        517      30     128

  Fragments arranged by size: 

                 517     128      30
 
BseYI C'CCAG_C

Cuts at:      0       3      24     228     675 
   Size:          3      21     204     447

  Fragments arranged by size: 

                 447     204      21       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     433     675 
   Size:        292     141     242

  Fragments arranged by size: 

                 292     242     141
 
BsiEI CG_ry'CG

Cuts at:      0     248     675 
   Size:        248     427
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     236     317     583     675 
   Size:        137      99      81     266      92

  Fragments arranged by size: 

                 266     137      99      92      81
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     264     675 
   Size:        264     411
 
BsrFI r'CCGG_y

Cuts at:      0     102     243     675 
   Size:        102     141     432

  Fragments arranged by size: 

                 432     141     102
 
BstEII G'GTnAC_C

Cuts at:      0     276     675 
   Size:        276     399
 
BstYI r'GATC_y

Cuts at:      0     173     327     401     675 
   Size:        173     154      74     274

  Fragments arranged by size: 

                 274     173     154      74
 
BtgI C'CryG_G

Cuts at:      0     248     675 
   Size:        248     427
 
BtsI GCAGTG_nn'

Cuts at:      0     113     675 
   Size:        113     562
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      75     110     675 
   Size:         75      35     565

  Fragments arranged by size: 

                 565      75      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     294     487     675 
   Size:        294     193     188

  Fragments arranged by size: 

                 294     193     188
 
EaeI y'GGCC_r

Cuts at:      0     206     245     675 
   Size:        206      39     430

  Fragments arranged by size: 

                 430     206      39
 
EagI C'GGCC_G

Cuts at:      0     245     675 
   Size:        245     430
 
EarI CTCTTCn'nnn_

Cuts at:      0     154     607     675 
   Size:        154     453      68

  Fragments arranged by size: 

                 453     154      68
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     373     394     675 
   Size:         61     312      21     281

  Fragments arranged by size: 

                 312     281      61      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     232     233     313     339     468     579     580     675 
   Size:        232       1      80      26     129     111       1      95

  Fragments arranged by size: 

                 232     129     111      95      80      26       1       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     183     423     675 
   Size:        183     240     252

  Fragments arranged by size: 

                 252     240     183
 
HaeII r_GCGC'y

Cuts at:      0      61     151     521     559     675 
   Size:         61      90     370      38     116

  Fragments arranged by size: 

                 370     116      90      61      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     386     418     675 
   Size:        386      32     257

  Fragments arranged by size: 

                 386     257      32
 
KasI G'GCGC_C

Cuts at:      0     147     675 
   Size:        147     528
 
MluI A'CGCG_T

Cuts at:      0      14     675 
   Size:         14     661
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     278     675 
   Size:        101     177     397

  Fragments arranged by size: 

                 397     177     101
 
MscI TGG'CCA

Cuts at:      0     208     675 
   Size:        208     467
 
MspA1I CmG'CkG

Cuts at:      0      36     183     434     675 
   Size:         36     147     251     241

  Fragments arranged by size: 

                 251     241     147      36
 
NaeI GCC'GGC

Cuts at:      0     104     675 
   Size:        104     571
 
NarI GG'CG_CC

Cuts at:      0     148     675 
   Size:        148     527
 
NgoMIV G'CCGG_C

Cuts at:      0     102     675 
   Size:        102     573
 
PpuMI rG'GwC_Cy

Cuts at:      0     339     675 
   Size:        339     336
 
PspOMI G'GGCC_C

Cuts at:      0     232     313     579     675 
   Size:        232      81     266      96

  Fragments arranged by size: 

                 266     232      96      81
 
PvuII CAG'CTG

Cuts at:      0      36     183     675 
   Size:         36     147     492

  Fragments arranged by size: 

                 492     147      36
 
SapI GCTCTTCn'nnn_

Cuts at:      0     154     675 
   Size:        154     521
 
SexAI A'CCwGG_T

Cuts at:      0     359     675 
   Size:        359     316
 
SfoI GGC'GCC

Cuts at:      0     149     675 
   Size:        149     526
 
SmlI C'TyrA_G

Cuts at:      0     439     675 
   Size:        439     236
 
TatI w'GTAC_w

Cuts at:      0     212     675 
   Size:        212     463
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     616     648     675 
   Size:        616      32      27

  Fragments arranged by size: 

                 616      32      27
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI    BglII Bme1580I     BpmI    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI Bsp1286I    BspMI    BsrFI
   BstEII    BstYI     BtgI     BtsI    CspCI     DrdI     EaeI     EagI
     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I     KasI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI   NgoMIV    PpuMI   PspOMI
    PvuII     SapI    SexAI     SfoI     SmlI     TatI     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BseRI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MslI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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