################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AclI AcsI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseRI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 539 GTCTAC GT^[AC][GT]AC AccIII 98 TCCGGA T^CCGGA Alw44I 292 GTGCAC G^TGCAC ApaLI 292 GTGCAC G^TGCAC AspHI 292 GTGCAC G[AT]GC[AT]^C AspI 936 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 857 GGGCTC G[GA]GC[CT]^C BpmI 380 CTGGAG CTGGAG(16/14) BsaI 353 GAGACC GGTCTC(1/5) BsaWI 98 TCCGGA [AT]^CCGG[AT] BseAI 98 TCCGGA T^CCGGA BsgI 43 GTGCAG GTGCAG(16/14) BsiHKAI 292 GTGCAC G[AT]GC[AT]^C BsmBI 127 GAGACG CGTCTC(1/5) BspEI 98 TCCGGA T^CCGGA BspMI 902 ACCTGC ACCTGC(4/8) BstF5I 826 GGATG GGATG(2/0) DsaI 38 CCGCGG C^C[GA][CT]GG EcoT14I 907 CCAAGG C^C[AT][AT]GG FokI 826 GGATG GGATG(9/13) HincII 78 GTTGAC GT[CT]^[GA]AC HindII 78 GTTGAC GT[CT]^[GA]AC KspI 38 CCGCGG CCGC^GG MaeII 228 ACGT A^CGT MroI 98 TCCGGA T^CCGGA MseI 512 TTAA T^TAA MspA1I 38 CCGCGG C[AC]G^C[GT]G PflFI 936 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacII 38 CCGCGG CCGC^GG SspI 650 AATATT AAT^ATT StuI 191 AGGCCT AGG^CCT StyI 907 CCAAGG C^C[AT][AT]GG TfiI 888 GATTC G^A[AT]TC Tru9I 512 TTAA T^TAA Tsp45I 947 GTGAC ^GT[GC]AC Tth111I 936 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 39 CGCG CG^CG 205 CGCG BalI 113 TGGCCA TGG^CCA 645 TGGCCA BamHI 71 GGATCC G^GATCC 101 GGATCC BcnI 178 CCCGG CC^[GC]GG 734 CCCGG BmyI 292 GTGCAC G[AGT]GC[ACT]^C 857 GGGCTC BsmFI 673 GTCCC GGGAC(10/14) 732 GTCCC Bsp1286I 292 GTGCAC G[AGT]GC[ACT]^C 857 GGGCTC BstUI 39 CGCG CG^CG 205 CGCG BstYI 71 GGATCC [GA]^GATC[CT] 101 GGATCC Cac8I 193 GCCTGC GC[ACGT]^[ACGT]GC 843 GCTGGC CfoI 206 GCGC GCG^C 831 GCGC DraII 375 AGGTCCT [GA]G^G[ACGT]CC[CT] 899 AGGACCT EcoO109I 375 AGGTCCT [GA]G^G[ACGT]CC[CT] 899 AGGACCT HhaI 206 GCGC GCG^C 831 GCGC HinP1I 206 GCGC G^CGC 831 GCGC HphI 90 GGTGA GGTGA(8/7) 281 TCACC MflI 71 GGATCC [GA]^GATC[CT] 101 GGATCC MluNI 113 TGGCCA TGG^CCA 645 TGGCCA MscI 113 TGGCCA TGG^CCA 645 TGGCCA MslI 153 CATGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 249 CATAATGGTG MvnI 39 CGCG CG^CG 205 CGCG NciI 178 CCCGG CC^[GC]GG 734 CCCGG NlaIII 153 CATG CATG^ 768 CATG PpuMI 375 AGGTCCT [GA]G^G[AT]CC[CT] 899 AGGACCT PstI 172 CTGCAG CTGCA^G 460 CTGCAG SfcI 172 CTGCAG C^T[GA][CT]AG 460 CTGCAG Tsp509I 630 AATT ^AATT 925 AATT XhoII 71 GGATCC [GA]^GATC[CT] 101 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbsI BbvI BfaI BpuAI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeI MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI