(Circular) (Six-base) MAPSORT of: pF1KE2443.seq Check: 6206 from: 1 to: 4076 pF1KE2443 4076 bp With 182 enzymes: SgfI * January 7, 2013 14:38 .. AccI GT'mk_AC Cuts at: 648 1114 648 Size: 466 3610 Acc65I G'GTAC_C Cuts at: 1092 1092 Size: 4076 AclI AA'CG_TT Cuts at: 3916 3916 Size: 4076 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1901 2333 3080 3348 1901 Size: 432 747 268 2629 Fragments arranged by size: 2629 747 432 268 AflIII A'CryG_T Cuts at: 1428 2532 3509 1428 Size: 1104 977 1995 Fragments arranged by size: 1995 1104 977 AgeI A'CCGG_T Cuts at: 2464 2464 Size: 4076 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 295 295 Size: 4076 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 727 759 727 Size: 32 4044 AlwNI CAG_nnn'CTG Cuts at: 1381 2948 1381 Size: 1567 2509 ApaLI G'TGCA_C Cuts at: 400 2846 400 Size: 2446 1630 ApoI r'AATT_y Cuts at: 1080 3429 3685 1080 Size: 2349 256 1471 Fragments arranged by size: 2349 1471 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4076 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4076 AvaI C'yCGr_G Cuts at: 1096 1096 Size: 4076 BamHI G'GATC_C Cuts at: 179 209 1101 179 Size: 30 892 3154 Fragments arranged by size: 3154 892 30 BanI G'GyrC_C Cuts at: 1092 1506 1754 1789 1092 Size: 414 248 35 3379 Fragments arranged by size: 3379 414 248 35 BanII G_rGCy'C Cuts at: 969 1090 2120 969 Size: 121 1030 2925 Fragments arranged by size: 2925 1030 121 BbeI G_GCGC'C Cuts at: 1510 1758 1510 Size: 248 3828 BbsI GAAGACnn'nnnn_ Cuts at: 339 435 862 339 Size: 96 427 3553 Fragments arranged by size: 3553 427 96 BciVI GTATCCnnnnn_n' Cuts at: 1974 2735 4076 1974 Size: 761 1341 1974 Fragments arranged by size: 1974 1341 761 BclI T'GATC_A Cuts at: 1596 1596 Size: 4076 BglII A'GATC_T Cuts at: 1591 3628 1591 Size: 2037 2039 BlpI GC'TnA_GC Cuts at: 1188 1188 Size: 4076 Bme1580I G_kGCm'C Cuts at: 404 1701 1794 2850 404 Size: 1297 93 1056 1630 Fragments arranged by size: 1630 1297 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 351 368 506 1288 1369 1704 3848 351 Size: 17 138 782 81 335 2144 579 Fragments arranged by size: 2144 782 579 335 138 81 17 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2443 2443 Size: 4076 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 509 509 Size: 4076 Bpu10I CC'TnA_GC Cuts at: 968 968 Size: 4076 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1252 2131 2623 2921 1252 Size: 879 492 298 2407 Fragments arranged by size: 2407 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 455 3780 455 Size: 3325 751 BsaAI yAC'GTr Cuts at: 1429 1429 Size: 4076 BsaBI GATnn'nnATC Cuts at: 6 1590 3627 6 Size: 1584 2037 455 Fragments arranged by size: 2037 1584 455 BsaHI Gr'CG_yC Cuts at: 1507 1755 2457 3953 1507 Size: 248 702 1496 1630 Fragments arranged by size: 1630 1496 702 248 BsaWI w'CCGG_w Cuts at: 206 1265 1786 2464 2738 2885 3296 206 Size: 1059 521 678 274 147 411 986 Fragments arranged by size: 1059 986 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 226 256 226 Size: 30 4046 BseYI C'CCAG_C Cuts at: 1501 2259 2836 3332 1501 Size: 758 577 496 2245 Fragments arranged by size: 2245 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 172 172 Size: 4076 BsiEI CG_ry'CG Cuts at: 104 1618 1664 2872 104 Size: 1514 46 1208 1308 Fragments arranged by size: 1514 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 404 1090 1868 2058 2850 404 Size: 686 778 190 792 1630 Fragments arranged by size: 1630 792 778 686 190 BsmBI CGTCTCn'nnnn_ Cuts at: 229 229 Size: 4076 Bsp1286I G_dGCh'C Cuts at: 404 969 1090 1701 1794 1868 2058 2120 2850 Size: 565 121 611 93 74 190 62 730 Cuts at: 2850 404 Size: 1630 Fragments arranged by size: 1630 730 611 565 190 121 93 74 62 BspEI T'CCGG_A Cuts at: 206 3296 206 Size: 3090 986 BspHI T'CATG_A Cuts at: 3252 3252 Size: 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1019 1126 1340 1642 2023 1019 Size: 107 214 302 381 3072 Fragments arranged by size: 3072 381 302 214 107 BsrBI CCG'CTC Cuts at: 50 2368 2422 3906 4071 50 Size: 2318 54 1484 165 55 Fragments arranged by size: 2318 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1988 1988 Size: 4076 BsrFI r'CCGG_y Cuts at: 2074 2255 2464 2074 Size: 181 209 3686 Fragments arranged by size: 3686 209 181 BssSI C'ACGA_G Cuts at: 2347 2705 2347 Size: 358 3718 BstAPI GCAn_nnn'nTGC Cuts at: 1453 1453 Size: 4076 BstBI TT'CG_AA Cuts at: 2437 2437 Size: 4076 BstYI r'GATC_y Cuts at: 1 179 209 1101 1568 1591 1926 2172 3184 Size: 178 30 892 467 23 335 246 1012 Cuts at: 3184 3270 3282 3628 1 Size: 86 12 346 449 Fragments arranged by size: 1012 892 467 449 346 335 246 178 86 30 23 12 BtgI C'CryG_G Cuts at: 107 146 107 Size: 39 4037 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2026 2207 2370 2026 Size: 181 163 3732 Fragments arranged by size: 3732 181 163 BtsI GCAGTG_nn' Cuts at: 516 653 1319 516 Size: 137 666 3273 Fragments arranged by size: 3273 666 137 DraI TTT'AAA Cuts at: 1075 1075 Size: 4076 DrdI GACnn_nn'nnGTC Cuts at: 1782 2640 1782 Size: 858 3218 EaeI y'GGCC_r Cuts at: 221 753 844 1661 1835 2226 2253 3598 221 Size: 532 91 817 174 391 27 1345 699 Fragments arranged by size: 1345 817 699 532 391 174 91 27 EagI C'GGCC_G Cuts at: 1661 1661 Size: 4076 EarI CTCTTCn'nnn_ Cuts at: 2099 2309 2099 Size: 210 3866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 399 2284 2594 2740 3886 399 Size: 1885 310 146 1146 589 Fragments arranged by size: 1885 1146 589 310 146 EcoICRI GAG'CTC Cuts at: 1088 1088 Size: 4076 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 509 1901 2333 3080 3348 509 Size: 1392 432 747 268 1237 Fragments arranged by size: 1392 1237 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 484 1008 1215 484 Size: 524 207 3345 Fragments arranged by size: 3345 524 207 EcoRI G'AATT_C Cuts at: 1080 1080 Size: 4076 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 879 2095 879 Size: 1216 2860 FspI TGC'GCA Cuts at: 1857 1857 Size: 4076 HaeII r_GCGC'y Cuts at: 1510 1758 2780 3759 1510 Size: 248 1022 979 1827 Fragments arranged by size: 1827 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1918 1948 1950 1980 1918 Size: 30 2 30 4014 Fragments arranged by size: 4014 30 30 2 HincII GTy'rAC Cuts at: 188 1115 188 Size: 927 3149 KasI G'GCGC_C Cuts at: 1506 1754 1506 Size: 248 3828 KpnI G_GTAC'C Cuts at: 1096 1096 Size: 4076 MluI A'CGCG_T Cuts at: 3509 3509 Size: 4076 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2747 2931 2747 Size: 184 3892 MscI TGG'CCA Cuts at: 223 755 1837 223 Size: 532 1082 2462 Fragments arranged by size: 2462 1082 532 MslI CAynn'nnrTG Cuts at: 265 361 2065 2192 265 Size: 96 1704 127 2149 Fragments arranged by size: 2149 1704 127 96 MspA1I CmG'CkG Cuts at: 148 1187 1501 1861 2874 3119 148 Size: 1039 314 360 1013 245 1105 Fragments arranged by size: 1105 1039 1013 360 314 245 NaeI GCC'GGC Cuts at: 2257 2257 Size: 4076 NarI GG'CG_CC Cuts at: 1507 1755 1507 Size: 248 3828 NcoI C'CATG_G Cuts at: 107 107 Size: 4076 NgoMIV G'CCGG_C Cuts at: 2255 2255 Size: 4076 NspI r_CATG'y Cuts at: 1129 1629 2536 1129 Size: 500 907 2669 Fragments arranged by size: 2669 907 500 PasI CC'CwG_GG Cuts at: 784 784 Size: 4076 PciI A'CATG_T Cuts at: 2532 2532 Size: 4076 PfoI T'CCnGG_A Cuts at: 486 486 Size: 4076 PmeI GTTT'AAAC Cuts at: 1075 1075 Size: 4076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3215 3247 3215 Size: 32 4044 PpuMI rG'GwC_Cy Cuts at: 484 1008 484 Size: 524 3552 PstI C_TGCA'G Cuts at: 284 572 1123 1808 284 Size: 288 551 685 2552 Fragments arranged by size: 2552 685 551 288 PvuI CG_AT'CG Cuts at: 104 104 Size: 4076 PvuII CAG'CTG Cuts at: 1501 1861 1501 Size: 360 3716 RsrII CG'GwC_CG Cuts at: 2271 2271 Size: 4076 SacI G_AGCT'C Cuts at: 1090 1090 Size: 4076 SacII CC_GC'GG Cuts at: 149 149 Size: 4076 SalI G'TCGA_C Cuts at: 1113 1113 Size: 4076 SapI GCTCTTCn'nnn_ Cuts at: 2099 2309 2099 Size: 210 3866 SbfI CC_TGCA'GG Cuts at: 1123 1123 Size: 4076 ScaI AGT'ACT Cuts at: 3593 3593 Size: 4076 SfcI C'TryA_G Cuts at: 33 280 568 1119 1804 2797 2988 33 Size: 247 288 551 685 993 191 1121 Fragments arranged by size: 1121 993 685 551 288 247 191 SfoI GGC'GCC Cuts at: 1508 1756 1508 Size: 248 3828 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4076 SmaI CCC'GGG Cuts at: 1098 1098 Size: 4076 SmlI C'TyrA_G Cuts at: 1231 2146 2638 2900 1231 Size: 915 492 262 2407 Fragments arranged by size: 2407 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4076 SphI G_CATG'C Cuts at: 1129 1629 1129 Size: 500 3576 SspI AAT'ATT Cuts at: 760 760 Size: 4076 StuI AGG'CCT Cuts at: 301 301 Size: 4076 StyI C'CwwG_G Cuts at: 107 1015 1210 1564 107 Size: 908 195 354 2619 Fragments arranged by size: 2619 908 354 195 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 653 1659 1792 2251 2460 653 Size: 1006 133 459 209 2269 Fragments arranged by size: 2269 1006 459 209 133 TatI w'GTAC_w Cuts at: 903 3591 903 Size: 2688 1388 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3593 3625 3593 Size: 32 4044 Tth111I GACn'n_nGTC Cuts at: 1047 1873 1047 Size: 826 3250 XbaI T'CTAG_A Cuts at: 1107 1107 Size: 4076 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1571 1571 Size: 4076 XmaI C'CCGG_G Cuts at: 1096 1096 Size: 4076 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BseRI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI