################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AocI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoT22I FseI FspI HincII HindII HpaI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 867 GGTACC G^GTACC AccI 157 GTCTAC GT^[AC][GT]AC AcsI 396 GAATTC [GA]^AATT[CT] Alw44I 1183 GTGCAC G^TGCAC AlwNI 415 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 359 AGCGCT AGC^GCT ApaLI 1183 GTGCAC G^TGCAC ApoI 396 GAATTC [GA]^AATT[CT] Asp718I 867 GGTACC G^GTACC BanII 643 GAGCCC G[GA]GC[CT]^C BbeI 1122 GGCGCC GGCGC^C BbrPI 52 CACGTG CAC^GTG BclI 902 TGATCA T^GATCA BfaI 592 CTAG C^TAG BglI 1172 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 52 CACGTG [CT]AC^GT[GA] Bsp1407I 1154 TGTACA T^GTACA BspDI 454 ATCGAT AT^CGAT BsrGI 1154 TGTACA T^GTACA BssSI 832 CTCGTG CACGAG(-5/-1) ClaI 454 ATCGAT AT^CGAT EarI 1035 GAAGAG CTCTTC(1/4) Eco47III 359 AGCGCT AGC^GCT EcoRI 396 GAATTC G^AATTC EcoRV 556 GATATC GAT^ATC FbaI 902 TGATCA T^GATCA HindIII 82 AAGCTT A^AGCTT KasI 1122 GGCGCC G^GCGCC KpnI 867 GGTACC GGTAC^C Ksp632I 1035 GAAGAG CTCTTC(1/4) KspI 1167 CCGCGG CCGC^GG MaeI 592 CTAG C^TAG MseI 214 TTAA T^TAA NarI 1122 GGCGCC GG^CGCC PflMI 221 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 52 CACGTG CAC^GTG PmlI 52 CACGTG CAC^GTG PshAI 990 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 1167 CCGCGG CCGC^GG ScaI 884 AGTACT AGT^ACT SfoI 1122 GGCGCC GGC^GCC SmaI 1042 CCCGGG CCC^GGG SspBI 1154 TGTACA T^GTACA TaqI 455 TCGA T^CGA Tru9I 214 TTAA T^TAA Tsp509I 397 AATT ^AATT TthHB8I 455 TCGA T^CGA Van91I 221 CCAACATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 899 CCATGATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1042 CCCGGG C^CCGGG XmaI 1042 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 53 ACGTGT A^C[GA][CT]GT 386 ACGTGT AluI 83 AGCT AG^CT 95 AGCT AvaI 1042 CCCGGG C^[CT]CG[GA]G 1279 CCCGAG BanI 867 GGTACC G^G[CT][GA]CC 1122 GGCGCC BpmI 425 CTCCAG CTGGAG(16/14) 1309 CTGGAG BseRI 612 GAGGAG GAGGAG(10/8) 1312 GAGGAG BsoBI 1042 CCCGGG C^[CT]CG[GA]G 1279 CCCGAG BsrDI 409 CATTGC GCAATG(2/0) 1326 CATTGC BstAPI 1226 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1254 GCACCTCCTGC BstXI 1301 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1472 CCAACTTCGTGG BstYI 534 GGATCT [GA]^GATC[CT] 1304 AGATCC DdeI 169 CTGAG C^T[ACGT]AG 839 CTGAG EcoNI 116 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1308 CCTGGAGGAGG EcoT14I 116 CCTTGG C^C[AT][AT]GG 530 CCTTGG MflI 534 GGATCT [GA]^GATC[CT] 1304 AGATCC StyI 116 CCTTGG C^C[AT][AT]GG 530 CCTTGG XhoII 534 GGATCT [GA]^GATC[CT] 1304 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AcyI AfaI AlwI AspHI AvaII BalI BbsI BbvI BcnI BmyI BpuAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI TseI Tsp45I TspRI