Restriction map for
pF1KB8480
(Circular) (Six-base) MAPSORT of: pF1KB8480.seq Check: 2228 from: 1 to: 5675
pF1KB8480 5675 bp
With 182 enzymes: SgfI *
August 25, 2016 10:58 ..
AccI GT'mk_AC
Cuts at: 1640 2666 2713 1640
Size: 1026 47 4602
Fragments arranged by size:
4602 1026 47
Acc65I G'GTAC_C
Cuts at: 281 2691 281
Size: 2410 3265
AclI AA'CG_TT
Cuts at: 5515 5515
Size: 5675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 387 1199 2092 2484 2633 3500 3932 4679
Size: 133 812 893 392 149 867 432 747
Cuts at: 4679 4947 254
Size: 268 982
Fragments arranged by size:
982 893 867 812 747 432 392 268
149 133
AflII C'TTAA_G
Cuts at: 2409 2409
Size: 5675
AflIII A'CryG_T
Cuts at: 3027 4131 5108 3027
Size: 1104 977 3594
Fragments arranged by size:
3594 1104 977
AgeI A'CCGG_T
Cuts at: 4063 4063
Size: 5675
AleI CACnn'nnGTG
Cuts at: 745 745
Size: 5675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 863 863
Size: 5675
AlwNI CAG_nnn'CTG
Cuts at: 170 2428 2980 4547 170
Size: 2258 552 1567 1298
Fragments arranged by size:
2258 1567 1298 552
ApaI G_GGCC'C
Cuts at: 152 152
Size: 5675
ApaLI G'TGCA_C
Cuts at: 4445 4445
Size: 5675
ApoI r'AATT_y
Cuts at: 624 1405 1480 1694 1716 1780 2077 2679 5028
Size: 781 75 214 22 64 297 602 2349
Cuts at: 5028 5284 624
Size: 256 1015
Fragments arranged by size:
2349 1015 781 602 297 256 214 75
64 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 5675
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5675
AvaI C'yCGr_G
Cuts at: 2695 2695
Size: 5675
BamHI G'GATC_C
Cuts at: 210 328 2700 210
Size: 118 2372 3185
Fragments arranged by size:
3185 2372 118
BanI G'GyrC_C
Cuts at: 267 281 2691 3105 3353 3388 267
Size: 14 2410 414 248 35 2554
Fragments arranged by size:
2554 2410 414 248 35 14
BanII G_rGCy'C
Cuts at: 152 617 1343 1491 2045 2689 3719 152
Size: 465 726 148 554 644 1030 2108
Fragments arranged by size:
2108 1030 726 644 554 465 148
BbeI G_GCGC'C
Cuts at: 3109 3357 3109
Size: 248 5427
BbsI GAAGACnn'nnnn_
Cuts at: 1055 1196 1238 1832 1996 2095 2483 1055
Size: 141 42 594 164 99 388 4247
Fragments arranged by size:
4247 594 388 164 141 99 42
BbvCI CC'TCA_GC
Cuts at: 966 966
Size: 5675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5641
BciVI GTATCCnnnnn_n'
Cuts at: 744 3573 4334 5675 744
Size: 2829 761 1341 744
Fragments arranged by size:
2829 1341 761 744
BclI T'GATC_A
Cuts at: 569 782 923 2546 3195 569
Size: 213 141 1623 649 3049
Fragments arranged by size:
3049 1623 649 213 141
BfrBI ATG'CAT
Cuts at: 637 637
Size: 5675
BglI GCCn_nnn'nGGC
Cuts at: 541 541
Size: 5675
BglII A'GATC_T
Cuts at: 599 3190 5227 599
Size: 2591 2037 1047
Fragments arranged by size:
2591 2037 1047
BlpI GC'TnA_GC
Cuts at: 2787 2787
Size: 5675
Bme1580I G_kGCm'C
Cuts at: 152 270 3300 3393 4449 152
Size: 118 3030 93 1056 1378
Fragments arranged by size:
3030 1378 1056 118 93
BmgBI CAC'GTC
Cuts at: 369 1510 369
Size: 1141 4534
BmrI ACTGGGnnnn_n'
Cuts at: 549 668 1440 2887 2968 3303 5447 549
Size: 119 772 1447 81 335 2144 777
Fragments arranged by size:
2144 1447 777 772 335 119 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1812 4042 1812
Size: 2230 3445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2395 2395
Size: 5675
Bpu10I CC'TnA_GC
Cuts at: 463 966 463
Size: 503 5172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1888 2306 2851 3730 4222 4520 1888
Size: 418 545 879 492 298 3043
Fragments arranged by size:
3043 879 545 492 418 298
BsaI GGTCTCn'nnnn_
Cuts at: 1957 2398 5379 1957
Size: 441 2981 2253
Fragments arranged by size:
2981 2253 441
BsaAI yAC'GTr
Cuts at: 3028 3028
Size: 5675
BsaBI GATnn'nnATC
Cuts at: 6 3189 5226 6
Size: 3183 2037 455
Fragments arranged by size:
3183 2037 455
BsaHI Gr'CG_yC
Cuts at: 3106 3354 4056 5552 3106
Size: 248 702 1496 3229
Fragments arranged by size:
3229 1496 702 248
BsaWI w'CCGG_w
Cuts at: 650 2864 3385 4063 4337 4484 4895 650
Size: 2214 521 678 274 147 411 1430
Fragments arranged by size:
2214 1430 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 231 526 529 532 1408 1612 231
Size: 295 3 3 876 204 4294
Fragments arranged by size:
4294 876 295 204 3 3
BseYI C'CCAG_C
Cuts at: 3100 3858 4435 4931 3100
Size: 758 577 496 3844
Fragments arranged by size:
3844 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 869 1899 440
Size: 429 1030 4216
Fragments arranged by size:
4216 1030 429
BsiEI CG_ry'CG
Cuts at: 104 204 3217 3263 4471 104
Size: 100 3013 46 1208 1308
Fragments arranged by size:
3013 1308 1208 100 46
BsiHKAI G_wGCw'C
Cuts at: 617 1343 1515 2689 3467 3657 4449 617
Size: 726 172 1174 778 190 792 1843
Fragments arranged by size:
1843 1174 792 778 726 190 172
BsmI GAATG_Cn'
Cuts at: 376 376
Size: 5675
BsmBI CGTCTCn'nnnn_
Cuts at: 375 520 375
Size: 145 5530
Bsp1286I G_dGCh'C
Cuts at: 152 270 617 1343 1491 1515 2045 2689 3300
Size: 118 347 726 148 24 530 644 611
Cuts at: 3300 3393 3467 3657 3719 4449 152
Size: 93 74 190 62 730 1378
Fragments arranged by size:
1378 730 726 644 611 530 347 190
148 118 93 74 62 24
BspEI T'CCGG_A
Cuts at: 4895 4895
Size: 5675
BspHI T'CATG_A
Cuts at: 2195 2312 4851 2195
Size: 117 2539 3019
Fragments arranged by size:
3019 2539 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2725 2939 3241 3622 2725
Size: 214 302 381 4778
Fragments arranged by size:
4778 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3967 4021 5505 5670 50
Size: 3917 54 1484 165 55
Fragments arranged by size:
3917 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3587 3587
Size: 5675
BsrFI r'CCGG_y
Cuts at: 3673 3854 4063 3673
Size: 181 209 5285
Fragments arranged by size:
5285 209 181
BssSI C'ACGA_G
Cuts at: 3946 4304 3946
Size: 358 5317
BstAPI GCAn_nnn'nTGC
Cuts at: 550 3052 550
Size: 2502 3173
BstBI TT'CG_AA
Cuts at: 4036 4036
Size: 5675
BstYI r'GATC_y
Cuts at: 1 210 328 441 599 669 1650 2454 2511
Size: 209 118 113 158 70 981 804 57
Cuts at: 2511 2534 2700 3167 3190 3525 3771 4783 4869
Size: 23 166 467 23 335 246 1012 86
Cuts at: 4869 4881 5227 1
Size: 12 346 449
Fragments arranged by size:
1012 981 804 467 449 346 335 246
209 166 158 118 113 86 70 57
23 23 12
Bsu36I CC'TnA_GG
Cuts at: 479 479
Size: 5675
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1181 3625 3806 3969 1181
Size: 2444 181 163 2887
Fragments arranged by size:
2887 2444 181 163
BtsI GCAGTG_nn'
Cuts at: 386 1454 2918 386
Size: 1068 1464 3143
Fragments arranged by size:
3143 1464 1068
ClaI AT'CG_AT
Cuts at: 1328 1328
Size: 5675
DraI TTT'AAA
Cuts at: 2674 2674
Size: 5675
DraIII CAC_nnn'GTG
Cuts at: 1510 1510
Size: 5675
DrdI GACnn_nn'nnGTC
Cuts at: 2097 3381 4239 2097
Size: 1284 858 3533
Fragments arranged by size:
3533 1284 858
EaeI y'GGCC_r
Cuts at: 867 3260 3434 3825 3852 5197 867
Size: 2393 174 391 27 1345 1345
Fragments arranged by size:
2393 1345 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3260 3260
Size: 5675
EarI CTCTTCn'nnn_
Cuts at: 1211 1331 2135 2153 3698 3908 1211
Size: 120 804 18 1545 210 2978
Fragments arranged by size:
2978 1545 804 210 120 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1771 3883 4193 4339 5485 1771
Size: 2112 310 146 1146 1961
Fragments arranged by size:
2112 1961 1146 310 146
EcoICRI GAG'CTC
Cuts at: 615 1341 2687 615
Size: 726 1346 3603
Fragments arranged by size:
3603 1346 726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 387 1199 2092 2395 2484 2633 3500 3932
Size: 133 812 893 303 89 149 867 432
Cuts at: 3932 4679 4947 254
Size: 747 268 982
Fragments arranged by size:
982 893 867 812 747 432 303 268
149 133 89
EcoNI CCTnn'n_nnAGG
Cuts at: 436 436
Size: 5675
EcoO109I rG'GnC_Cy
Cuts at: 149 2814 149
Size: 2665 3010
EcoRI G'AATT_C
Cuts at: 1405 1480 2679 1405
Size: 75 1199 4401
Fragments arranged by size:
4401 1199 75
EcoRV GAT'ATC
Cuts at: 855 1120 855
Size: 265 5410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 795 3694 795
Size: 2899 2776
FspI TGC'GCA
Cuts at: 3456 3456
Size: 5675
HaeII r_GCGC'y
Cuts at: 3109 3357 4379 5358 3109
Size: 248 1022 979 3426
Fragments arranged by size:
3426 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1210 1242 1356 1388 1565 1597 1884 1916 2632
Size: 32 114 32 177 32 287 32 716
Cuts at: 2632 2664 3517 3547 3549 3579 1210
Size: 32 853 30 2 30 3306
Fragments arranged by size:
3306 853 716 287 177 114 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 276 2714 276
Size: 2438 3237
HindIII A'AGCT_T
Cuts at: 660 833 1810 1846 660
Size: 173 977 36 4489
Fragments arranged by size:
4489 977 173 36
HpaI GTT'AAC
Cuts at: 276 276
Size: 5675
KasI G'GCGC_C
Cuts at: 3105 3353 3105
Size: 248 5427
KpnI G_GTAC'C
Cuts at: 285 2695 285
Size: 2410 3265
MluI A'CGCG_T
Cuts at: 5108 5108
Size: 5675
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1208 1457 2088 4346 4530 1208
Size: 249 631 2258 184 2353
Fragments arranged by size:
2353 2258 631 249 184
MscI TGG'CCA
Cuts at: 869 3436 869
Size: 2567 3108
MslI CAynn'nnrTG
Cuts at: 745 938 1921 2259 3664 3791 745
Size: 193 983 338 1405 127 2629
Fragments arranged by size:
2629 1405 983 338 193 127
MspA1I CmG'CkG
Cuts at: 2070 2786 3100 3460 4473 4718 2070
Size: 716 314 360 1013 245 3027
Fragments arranged by size:
3027 1013 716 360 314 245
NaeI GCC'GGC
Cuts at: 3856 3856
Size: 5675
NarI GG'CG_CC
Cuts at: 3106 3354 3106
Size: 248 5427
NdeI CA'TA_TG
Cuts at: 2283 2283
Size: 5675
NgoMIV G'CCGG_C
Cuts at: 3854 3854
Size: 5675
NsiI A_TGCA'T
Cuts at: 639 639
Size: 5675
NspI r_CATG'y
Cuts at: 2527 2728 3228 4135 2527
Size: 201 500 907 4067
Fragments arranged by size:
4067 907 500 201
PciI A'CATG_T
Cuts at: 4131 4131
Size: 5675
PflMI CCAn_nnn'nTGG
Cuts at: 507 1430 507
Size: 923 4752
PmeI GTTT'AAAC
Cuts at: 2674 2674
Size: 5675
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4814 4846 4814
Size: 32 5643
PspOMI G'GGCC_C
Cuts at: 148 148
Size: 5675
PstI C_TGCA'G
Cuts at: 449 2722 3407 449
Size: 2273 685 2717
Fragments arranged by size:
2717 2273 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5675
PvuII CAG'CTG
Cuts at: 2070 3100 3460 2070
Size: 1030 360 4285
Fragments arranged by size:
4285 1030 360
RsrII CG'GwC_CG
Cuts at: 3870 3870
Size: 5675
SacI G_AGCT'C
Cuts at: 617 1343 2689 617
Size: 726 1346 3603
Fragments arranged by size:
3603 1346 726
SalI G'TCGA_C
Cuts at: 2712 2712
Size: 5675
SapI GCTCTTCn'nnn_
Cuts at: 1331 3698 3908 1331
Size: 2367 210 3098
Fragments arranged by size:
3098 2367 210
SbfI CC_TGCA'GG
Cuts at: 2722 2722
Size: 5675
ScaI AGT'ACT
Cuts at: 1063 2056 5192 1063
Size: 993 3136 1546
Fragments arranged by size:
3136 1546 993
SexAI A'CCwGG_T
Cuts at: 1973 1973
Size: 5675
SfcI C'TryA_G
Cuts at: 33 445 2538 2718 3403 4396 4587 33
Size: 412 2093 180 685 993 191 1121
Fragments arranged by size:
2093 1121 993 685 412 191 180
SfoI GGC'GCC
Cuts at: 3107 3355 3107
Size: 248 5427
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5675
SmaI CCC'GGG
Cuts at: 2697 2697
Size: 5675
SmlI C'TyrA_G
Cuts at: 1903 2321 2409 2830 3745 4237 4499 1903
Size: 418 88 421 915 492 262 3079
Fragments arranged by size:
3079 915 492 421 418 262 88
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5675
SphI G_CATG'C
Cuts at: 2728 3228 2728
Size: 500 5175
StuI AGG'CCT
Cuts at: 2117 2117
Size: 5675
StyI C'CwwG_G
Cuts at: 414 770 792 1967 2809 3163 414
Size: 356 22 1175 842 354 2926
Fragments arranged by size:
2926 1175 842 356 354 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3258 3391 3850 4059 3258
Size: 133 459 209 4874
Fragments arranged by size:
4874 459 209 133
TatI w'GTAC_w
Cuts at: 1061 1322 2054 5190 1061
Size: 261 732 3136 1546
Fragments arranged by size:
3136 1546 732 261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5192 5224 5192
Size: 32 5643
Tth111I GACn'n_nGTC
Cuts at: 3472 3472
Size: 5675
XbaI T'CTAG_A
Cuts at: 2706 2706
Size: 5675
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 825 877 2115 3170 825
Size: 52 1238 1055 3330
Fragments arranged by size:
3330 1238 1055 52
XmaI C'CCGG_G
Cuts at: 2695 2695
Size: 5675
XmnI GAAnn'nnTTC
Cuts at: 2003 2003
Size: 5675
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI AscI AvrII BaeI
BmtI BsaXI BsiWI BsrGI BssHII BstEII BstXI BstZ17I
BtgI CspCI FseI FspAI MfeI NcoI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI