Restriction map for pF1KB8480
 (Linear) (Six-base) MAPSORT of: KIBB8480.seq  Check: 6490  from: 1  to: 2562



KIBB8480.orf

 With 182 enzymes: SgfI * 

                        August 25, 2016 10:58  ..

AccI GT'mk_AC

Cuts at:      0    1532    2558    2562 
   Size:       1532    1026       4

  Fragments arranged by size: 

                1532    1026       4
 
Acc65I G'GTAC_C

Cuts at:      0     173    2562 
   Size:        173    2389
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     279    1091    1984    2376    2525    2562 
   Size:        146     133     812     893     392     149      37

  Fragments arranged by size: 

                 893     812     392     149     146     133      37
 
AflII C'TTAA_G

Cuts at:      0    2301    2562 
   Size:       2301     261
 
AleI CACnn'nnGTG

Cuts at:      0     637    2562 
   Size:        637    1925
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     755    2562 
   Size:        755    1807
 
AlwNI CAG_nnn'CTG

Cuts at:      0      62    2320    2562 
   Size:         62    2258     242

  Fragments arranged by size: 

                2258     242      62
 
ApaI G_GGCC'C

Cuts at:      0      44    2562 
   Size:         44    2518
 
ApoI r'AATT_y

Cuts at:      0     516    1297    1372    1586    1608    1672    1969    2562 
   Size:        516     781      75     214      22      64     297     593

  Fragments arranged by size: 

                 781     593     516     297     214      75      64      22
            
 
BamHI G'GATC_C

Cuts at:      0     102     220    2562 
   Size:        102     118    2342

  Fragments arranged by size: 

                2342     118     102
 
BanI G'GyrC_C

Cuts at:      0     159     173    2562 
   Size:        159      14    2389

  Fragments arranged by size: 

                2389     159      14
 
BanII G_rGCy'C

Cuts at:      0      44     509    1235    1383    1937    2562 
   Size:         44     465     726     148     554     625

  Fragments arranged by size: 

                 726     625     554     465     148      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     947    1088    1130    1724    1888    1987    2375    2562 
   Size:        947     141      42     594     164      99     388     187

  Fragments arranged by size: 

                 947     594     388     187     164     141      99      42
            
 
BbvCI CC'TCA_GC

Cuts at:      0     858    2562 
   Size:        858    1704
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     636    2562 
   Size:        636    1926
 
BclI T'GATC_A

Cuts at:      0     461     674     815    2438    2562 
   Size:        461     213     141    1623     124

  Fragments arranged by size: 

                1623     461     213     141     124
 
BfrBI ATG'CAT

Cuts at:      0     529    2562 
   Size:        529    2033
 
BglI GCCn_nnn'nGGC

Cuts at:      0     433    2562 
   Size:        433    2129
 
BglII A'GATC_T

Cuts at:      0     491    2562 
   Size:        491    2071
 
Bme1580I G_kGCm'C

Cuts at:      0      44     162    2562 
   Size:         44     118    2400

  Fragments arranged by size: 

                2400     118      44
 
BmgBI CAC'GTC

Cuts at:      0     261    1402    2562 
   Size:        261    1141    1160

  Fragments arranged by size: 

                1160    1141     261
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     441     560    1332    2562 
   Size:        441     119     772    1230

  Fragments arranged by size: 

                1230     772     441     119
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1704    2562 
   Size:       1704     858
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2287    2562 
   Size:       2287     275
 
Bpu10I CC'TnA_GC

Cuts at:      0     355     858    2562 
   Size:        355     503    1704

  Fragments arranged by size: 

                1704     503     355
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1780    2198    2562 
   Size:       1780     418     364

  Fragments arranged by size: 

                1780     418     364
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1849    2290    2562 
   Size:       1849     441     272

  Fragments arranged by size: 

                1849     441     272
 
BsaWI w'CCGG_w

Cuts at:      0     542    2562 
   Size:        542    2020
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     123     418     421     424    1300    1504    2562 
   Size:        123     295       3       3     876     204    1058

  Fragments arranged by size: 

                1058     876     295     204     123       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     761    1791    2562 
   Size:        332     429    1030     771

  Fragments arranged by size: 

                1030     771     429     332
 
BsiEI CG_ry'CG

Cuts at:      0      96    2562 
   Size:         96    2466
 
BsiHKAI G_wGCw'C

Cuts at:      0     509    1235    1407    2562 
   Size:        509     726     172    1155

  Fragments arranged by size: 

                1155     726     509     172
 
BsmI GAATG_Cn'

Cuts at:      0     268    2562 
   Size:        268    2294
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     267     412    2562 
   Size:        267     145    2150

  Fragments arranged by size: 

                2150     267     145
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     162     509    1235    1383    1407    1937    2562 
   Size:         44     118     347     726     148      24     530     625

  Fragments arranged by size: 

                 726     625     530     347     148     118      44      24
            
 
BspHI T'CATG_A

Cuts at:      0    2087    2204    2562 
   Size:       2087     117     358

  Fragments arranged by size: 

                2087     358     117
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     442    2562 
   Size:        442    2120
 
BstYI r'GATC_y

Cuts at:      0     102     220     333     491     561    1542    2346    2403 
   Size:        102     118     113     158      70     981     804      57

Cuts at:   2403    2426    2562 
   Size:         23     136

  Fragments arranged by size: 

                 981     804     158     136     118     113     102      70
                  57      23
 
Bsu36I CC'TnA_GG

Cuts at:      0     371    2562 
   Size:        371    2191
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1073    2562 
   Size:       1073    1489
 
BtsI GCAGTG_nn'

Cuts at:      0     278    1346    2562 
   Size:        278    1068    1216

  Fragments arranged by size: 

                1216    1068     278
 
ClaI AT'CG_AT

Cuts at:      0    1220    2562 
   Size:       1220    1342
 
DraIII CAC_nnn'GTG

Cuts at:      0    1402    2562 
   Size:       1402    1160
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1989    2562 
   Size:       1989     573
 
EaeI y'GGCC_r

Cuts at:      0     759    2562 
   Size:        759    1803
 
EarI CTCTTCn'nnn_

Cuts at:      0    1103    1223    2027    2045    2562 
   Size:       1103     120     804      18     517

  Fragments arranged by size: 

                1103     804     517     120      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1663    2562 
   Size:       1663     899
 
EcoICRI GAG'CTC

Cuts at:      0     507    1233    2562 
   Size:        507     726    1329

  Fragments arranged by size: 

                1329     726     507
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     279    1091    1984    2287    2376    2525    2562 
   Size:        146     133     812     893     303      89     149      37

  Fragments arranged by size: 

                 893     812     303     149     146     133      89      37
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     328    2562 
   Size:        328    2234
 
EcoO109I rG'GnC_Cy

Cuts at:      0      41    2562 
   Size:         41    2521
 
EcoRI G'AATT_C

Cuts at:      0    1297    1372    2562 
   Size:       1297      75    1190

  Fragments arranged by size: 

                1297    1190      75
 
EcoRV GAT'ATC

Cuts at:      0     747    1012    2562 
   Size:        747     265    1550

  Fragments arranged by size: 

                1550     747     265
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     687    2562 
   Size:        687    1875
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1102    1134    1248    1280    1457    1489    1776    1808 
   Size:       1102      32     114      32     177      32     287      32

Cuts at:   1808    2524    2556    2562 
   Size:        716      32       6

  Fragments arranged by size: 

                1102     716     287     177     114      32      32      32
                  32      32       6
 
HincII GTy'rAC

Cuts at:      0     168    2562 
   Size:        168    2394
 
HindIII A'AGCT_T

Cuts at:      0     552     725    1702    1738    2562 
   Size:        552     173     977      36     824

  Fragments arranged by size: 

                 977     824     552     173      36
 
HpaI GTT'AAC

Cuts at:      0     168    2562 
   Size:        168    2394
 
KpnI G_GTAC'C

Cuts at:      0     177    2562 
   Size:        177    2385
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1100    1349    1980    2562 
   Size:       1100     249     631     582

  Fragments arranged by size: 

                1100     631     582     249
 
MscI TGG'CCA

Cuts at:      0     761    2562 
   Size:        761    1801
 
MslI CAynn'nnrTG

Cuts at:      0     637     830    1813    2151    2562 
   Size:        637     193     983     338     411

  Fragments arranged by size: 

                 983     637     411     338     193
 
MspA1I CmG'CkG

Cuts at:      0    1962    2562 
   Size:       1962     600
 
NdeI CA'TA_TG

Cuts at:      0    2175    2562 
   Size:       2175     387
 
NsiI A_TGCA'T

Cuts at:      0     531    2562 
   Size:        531    2031
 
NspI r_CATG'y

Cuts at:      0    2419    2562 
   Size:       2419     143
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     399    1322    2562 
   Size:        399     923    1240

  Fragments arranged by size: 

                1240     923     399
 
PspOMI G'GGCC_C

Cuts at:      0      40    2562 
   Size:         40    2522
 
PstI C_TGCA'G

Cuts at:      0     341    2562 
   Size:        341    2221
 
PvuII CAG'CTG

Cuts at:      0    1962    2562 
   Size:       1962     600
 
SacI G_AGCT'C

Cuts at:      0     509    1235    2562 
   Size:        509     726    1327

  Fragments arranged by size: 

                1327     726     509
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1223    2562 
   Size:       1223    1339
 
ScaI AGT'ACT

Cuts at:      0     955    1948    2562 
   Size:        955     993     614

  Fragments arranged by size: 

                 993     955     614
 
SexAI A'CCwGG_T

Cuts at:      0    1865    2562 
   Size:       1865     697
 
SfcI C'TryA_G

Cuts at:      0     337    2430    2562 
   Size:        337    2093     132

  Fragments arranged by size: 

                2093     337     132
 
SmlI C'TyrA_G

Cuts at:      0    1795    2213    2301    2562 
   Size:       1795     418      88     261

  Fragments arranged by size: 

                1795     418     261      88
 
StuI AGG'CCT

Cuts at:      0    2009    2562 
   Size:       2009     553
 
StyI C'CwwG_G

Cuts at:      0     306     662     684    1859    2562 
   Size:        306     356      22    1175     703

  Fragments arranged by size: 

                1175     703     356     306      22
 
TatI w'GTAC_w

Cuts at:      0     953    1214    1946    2562 
   Size:        953     261     732     616

  Fragments arranged by size: 

                 953     732     616     261
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     717     769    2007    2562 
   Size:        717      52    1238     555

  Fragments arranged by size: 

                1238     717     555      52
 
XmnI GAAnn'nnTTC

Cuts at:      0    1895    2562 
   Size:       1895     667
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII     AleI     AlfI    AlwNI     ApaI
     ApoI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI   BstAPI    BstYI   Bsu36I    BtgZI
     BtsI     ClaI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII
  HindIII     HpaI     KpnI     MmeI     MscI     MslI   MspA1I     NdeI
     NsiI     NspI    PflMI   PspOMI     PstI    PvuII     SacI     SapI
     ScaI    SexAI     SfcI     SmlI     StuI     StyI     TatI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI   AflIII     AgeI     AhdI     AloI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI
     BcgI     BlpI     BmtI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI
    BsiWI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI    CspCI     DraI
     EagI     FseI     FspI    FspAI    HaeII     KasI     MfeI     MluI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI
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