Restriction map for pF1KE2480
 (Linear) (Six-base) MAPSORT of: KIEE2480.seq  Check: 6234  from: 1  to: 4359



KIEE2480.orf

 With 182 enzymes: SgfI * 

                        August 27, 2013 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3631    4359 
   Size:       3631     728
 
AccI GT'mk_AC

Cuts at:      0     892    3690    4359 
   Size:        892    2798     669

  Fragments arranged by size: 

                2798     892     669
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     580     743    1117    1694    2395    2578    2659 
   Size:        128     452     163     374     577     701     183      81

Cuts at:   2659    3122    3397    3644    4359 
   Size:        463     275     247     715

  Fragments arranged by size: 

                 715     701     577     463     452     374     275     247
                 183     163     128      81
 
AfeI AGC'GCT

Cuts at:      0     423    4359 
   Size:        423    3936
 
AflIII A'CryG_T

Cuts at:      0    1906    1936    2163    3113    3115    4359 
   Size:       1906      30     227     950       2    1244

  Fragments arranged by size: 

                1906    1244     950     227      30       2
 
AgeI A'CCGG_T

Cuts at:      0     887    2938    4359 
   Size:        887    2051    1421

  Fragments arranged by size: 

                2051    1421     887
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1544    4359 
   Size:       1544    2815
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1441    1692    2818    4326    4359 
   Size:       1441     251    1126    1508      33

  Fragments arranged by size: 

                1508    1441    1126     251      33
 
ApoI r'AATT_y

Cuts at:      0     323     638     870     985    1090    1637    2700    3053 
   Size:        323     315     232     115     105     547    1063     353

Cuts at:   3053    3512    3862    3988    4359 
   Size:        459     350     126     371

  Fragments arranged by size: 

                1063     547     459     371     353     350     323     315
                 232     126     115     105
 
AseI AT'TA_AT

Cuts at:      0     923    2197    4359 
   Size:        923    1274    2162

  Fragments arranged by size: 

                2162    1274     923
 
AvaI C'yCGr_G

Cuts at:      0    3341    4359 
   Size:       3341    1018
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2297    2330    4359 
   Size:       2297      33    2029

  Fragments arranged by size: 

                2297    2029      33
 
BanI G'GyrC_C

Cuts at:      0     884    4359 
   Size:        884    3475
 
BanII G_rGCy'C

Cuts at:      0    3342    4359 
   Size:       3342    1017
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     871    2341    3758    4359 
   Size:        871    1470    1417     601

  Fragments arranged by size: 

                1470    1417     871     601
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2298    3539    4359 
   Size:       2298    1241     820

  Fragments arranged by size: 

                2298    1241     820
 
BclI T'GATC_A

Cuts at:      0       2    4359 
   Size:          2    4357
 
BfrBI ATG'CAT

Cuts at:      0     482    1312    4359 
   Size:        482     830    3047

  Fragments arranged by size: 

                3047     830     482
 
BglII A'GATC_T

Cuts at:      0    3849    3958    4359 
   Size:       3849     109     401

  Fragments arranged by size: 

                3849     401     109
 
BlpI GC'TnA_GC

Cuts at:      0    2233    3501    4359 
   Size:       2233    1268     858

  Fragments arranged by size: 

                2233    1268     858
 
Bme1580I G_kGCm'C

Cuts at:      0    1914    4359 
   Size:       1914    2445
 
BmgBI CAC'GTC

Cuts at:      0    1939    4359 
   Size:       1939    2420
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1005    2996    4359 
   Size:       1005    1991    1363

  Fragments arranged by size: 

                1991    1363    1005
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517    1463    1940    3559    3718    4000    4027    4359 
   Size:        517     946     477    1619     159     282      27     332

  Fragments arranged by size: 

                1619     946     517     477     332     282     159      27
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    4114    4359 
   Size:       4114     245
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2259    2617    3292    4359 
   Size:       2259     358     675    1067

  Fragments arranged by size: 

                2259    1067     675     358
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3287    4182    4359 
   Size:       3287     895     177

  Fragments arranged by size: 

                3287     895     177
 
BsaAI yAC'GTr

Cuts at:      0    1909    3116    4359 
   Size:       1909    1207    1243

  Fragments arranged by size: 

                1909    1243    1207
 
BsaHI Gr'CG_yC

Cuts at:      0    4287    4359 
   Size:       4287      72
 
BsaWI w'CCGG_w

Cuts at:      0     887    2938    3021    4024    4359 
   Size:        887    2051      83    1003     335

  Fragments arranged by size: 

                2051    1003     887     335      83
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     293    1173    1570    4359 
   Size:        293     880     397    2789

  Fragments arranged by size: 

                2789     880     397     293
 
BseYI C'CCAG_C

Cuts at:      0     424    4359 
   Size:        424    3935
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    2754    4359 
   Size:        949    1805    1605

  Fragments arranged by size: 

                1805    1605     949
 
BsiHKAI G_wGCw'C

Cuts at:      0    1731    3342    3616    4182    4359 
   Size:       1731    1611     274     566     177

  Fragments arranged by size: 

                1731    1611     566     274     177
 
BsmI GAATG_Cn'

Cuts at:      0    1016    4359 
   Size:       1016    3343
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4294    4359 
   Size:       4294      65
 
Bsp1286I G_dGCh'C

Cuts at:      0    1731    1914    3342    3616    4182    4359 
   Size:       1731     183    1428     274     566     177

  Fragments arranged by size: 

                1731    1428     566     274     183     177
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3558    3631    3659    4359 
   Size:       3558      73      28     700

  Fragments arranged by size: 

                3558     700      73      28
 
BsrDI GCAATG_nn'

Cuts at:      0    3200    4042    4359 
   Size:       3200     842     317

  Fragments arranged by size: 

                3200     842     317
 
BsrFI r'CCGG_y

Cuts at:      0     887    2938    4359 
   Size:        887    2051    1421

  Fragments arranged by size: 

                2051    1421     887
 
BsrGI T'GTAC_A

Cuts at:      0    1319    4359 
   Size:       1319    3040
 
BssSI C'ACGA_G

Cuts at:      0    3655    4359 
   Size:       3655     704
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     298    2732    4326    4338    4359 
   Size:        298    2434    1594      12      21

  Fragments arranged by size: 

                2434    1594     298      21      12
 
BstEII G'GTnAC_C

Cuts at:      0    3040    4359 
   Size:       3040    1319
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     318    4359 
   Size:        318    4041
 
BstYI r'GATC_y

Cuts at:      0    1569    2254    3849    3958    4359 
   Size:       1569     685    1595     109     401

  Fragments arranged by size: 

                1595    1569     685     401     109
 
BstZ17I GTA'TAC

Cuts at:      0    3691    4359 
   Size:       3691     668
 
BtgI C'CryG_G

Cuts at:      0     587    4086    4359 
   Size:        587    3499     273

  Fragments arranged by size: 

                3499     587     273
 
BtsI GCAGTG_nn'

Cuts at:      0     937    2742    3489    4359 
   Size:        937    1805     747     870

  Fragments arranged by size: 

                1805     937     870     747
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     101     136    4359 
   Size:        101      35    4223

  Fragments arranged by size: 

                4223     101      35
 
DraI TTT'AAA

Cuts at:      0     684    1043    1662    1881    4359 
   Size:        684     359     619     219    2478

  Fragments arranged by size: 

                2478     684     619     359     219
 
EaeI y'GGCC_r

Cuts at:      0      50     308    3486    4359 
   Size:         50     258    3178     873

  Fragments arranged by size: 

                3178     873     258      50
 
EarI CTCTTCn'nnn_

Cuts at:      0    1136    3098    3620    4359 
   Size:       1136    1962     522     739

  Fragments arranged by size: 

                1962    1136     739     522
 
EcoICRI GAG'CTC

Cuts at:      0    3340    4359 
   Size:       3340    1019
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     517     580     743    1117    1463    1694    1940 
   Size:        128     389      63     163     374     346     231     246

Cuts at:   1940    2395    2578    2659    3122    3397    3559    3644    3718 
   Size:        455     183      81     463     275     162      85      74

Cuts at:   3718    4000    4027    4359 
   Size:        282      27     332

  Fragments arranged by size: 

                 463     455     389     374     346     332     282     275
                 246     231     183     163     162     128      85      81
                  74      63      27
 
EcoRI G'AATT_C

Cuts at:      0     870    1637    4359 
   Size:        870     767    2722

  Fragments arranged by size: 

                2722     870     767
 
EcoRV GAT'ATC

Cuts at:      0    2894    4359 
   Size:       2894    1465
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2253    2406    4359 
   Size:       2253     153    1953

  Fragments arranged by size: 

                2253    1953     153
 
HaeII r_GCGC'y

Cuts at:      0     425    4359 
   Size:        425    3934
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1530    1562    2529    2561    2590    2622    2664    2696 
   Size:       1530      32     967      32      29      32      42      32

Cuts at:   2696    2973    3005    3265    3297    4359 
   Size:        277      32     260      32    1062

  Fragments arranged by size: 

                1530    1062     967     277     260      42      32      32
                  32      32      32      32      29
 
HincII GTy'rAC

Cuts at:      0    2455    2744    3174    3298    4359 
   Size:       2455     289     430     124    1061

  Fragments arranged by size: 

                2455    1061     430     289     124
 
HindIII A'AGCT_T

Cuts at:      0     392    2235    4359 
   Size:        392    1843    2124

  Fragments arranged by size: 

                2124    1843     392
 
HpaI GTT'AAC

Cuts at:      0    2744    4359 
   Size:       2744    1615
 
MfeI C'AATT_G

Cuts at:      0    1424    1764    2002    4359 
   Size:       1424     340     238    2357

  Fragments arranged by size: 

                2357    1424     340     238
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3730    4359 
   Size:       3730     629
 
MscI TGG'CCA

Cuts at:      0      52     310    3488    4359 
   Size:         52     258    3178     871

  Fragments arranged by size: 

                3178     871     258      52
 
MslI CAynn'nnrTG

Cuts at:      0    2422    3143    4173    4359 
   Size:       2422     721    1030     186

  Fragments arranged by size: 

                2422    1030     721     186
 
MspA1I CmG'CkG

Cuts at:      0    3135    4164    4359 
   Size:       3135    1029     195

  Fragments arranged by size: 

                3135    1029     195
 
NcoI C'CATG_G

Cuts at:      0     587    4086    4359 
   Size:        587    3499     273

  Fragments arranged by size: 

                3499     587     273
 
NsiI A_TGCA'T

Cuts at:      0     484    1314    4359 
   Size:        484     830    3045

  Fragments arranged by size: 

                3045     830     484
 
NspI r_CATG'y

Cuts at:      0    1181    4359 
   Size:       1181    3178
 
PasI CC'CwG_GG

Cuts at:      0    3153    4359 
   Size:       3153    1206
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     847    4359 
   Size:        847    3512
 
PfoI T'CCnGG_A

Cuts at:      0    1169    1472    4359 
   Size:       1169     303    2887

  Fragments arranged by size: 

                2887    1169     303
 
PmlI CAC'GTG

Cuts at:      0    1909    3116    4359 
   Size:       1909    1207    1243

  Fragments arranged by size: 

                1909    1243    1207
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      74     106    4359 
   Size:         74      32    4253

  Fragments arranged by size: 

                4253      74      32
 
PsiI TTA'TAA

Cuts at:      0    1825    4359 
   Size:       1825    2534
 
PspXI vC'TCGA_Gb

Cuts at:      0    3341    4359 
   Size:       3341    1018
 
PstI C_TGCA'G

Cuts at:      0    1251    4331    4359 
   Size:       1251    3080      28

  Fragments arranged by size: 

                3080    1251      28
 
PvuII CAG'CTG

Cuts at:      0    4164    4359 
   Size:       4164     195
 
SacI G_AGCT'C

Cuts at:      0    3342    4359 
   Size:       3342    1017
 
ScaI AGT'ACT

Cuts at:      0    1593    4359 
   Size:       1593    2766
 
SexAI A'CCwGG_T

Cuts at:      0    1896    4359 
   Size:       1896    2463
 
SfcI C'TryA_G

Cuts at:      0    1247    2060    4327    4359 
   Size:       1247     813    2267      32

  Fragments arranged by size: 

                2267    1247     813      32
 
SmlI C'TyrA_G

Cuts at:      0    2238    2596    3271    3341    4359 
   Size:       2238     358     675      70    1018

  Fragments arranged by size: 

                2238    1018     675     358      70
 
SpeI A'CTAG_T

Cuts at:      0     771    3880    4359 
   Size:        771    3109     479

  Fragments arranged by size: 

                3109     771     479
 
SspI AAT'ATT

Cuts at:      0     297    1188    4359 
   Size:        297     891    3171

  Fragments arranged by size: 

                3171     891     297
 
StuI AGG'CCT

Cuts at:      0     286    4282    4359 
   Size:        286    3996      77

  Fragments arranged by size: 

                3996     286      77
 
StyI C'CwwG_G

Cuts at:      0      62     259     287     587    2498    4035    4086    4359 
   Size:         62     197      28     300    1911    1537      51     273

  Fragments arranged by size: 

                1911    1537     300     273     197      62      51      28
            
 
TatI w'GTAC_w

Cuts at:      0      24    1319    1591    1719    1836    3232    3267    3287 
   Size:         24    1295     272     128     117    1396      35      20

Cuts at:   3287    4359 
   Size:       1072

  Fragments arranged by size: 

                1396    1295    1072     272     128     117      35      24
                  20
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     171     203    4040    4072    4359 
   Size:        171      32    3837      32     287

  Fragments arranged by size: 

                3837     287     171      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1898    4359 
   Size:       1898    2461
 
XbaI T'CTAG_A

Cuts at:      0    1572    4359 
   Size:       1572    2787
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      60    1921    3978    4084    4359 
   Size:         60    1861    2057     106     275

  Fragments arranged by size: 

                2057    1861     275     106      60
 
XhoI C'TCGA_G

Cuts at:      0    3341    4359 
   Size:       3341    1018
 
XmnI GAAnn'nnTTC

Cuts at:      0    3410    4359 
   Size:       3410     949
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AgeI     AhdI    AlwNI
     ApoI     AseI     AvaI     BaeI     BanI    BanII     BbsI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I     BtgI
     BtsI    CspCI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PasI
    PflMI     PfoI     PmlI     PpiI     PsiI    PspXI     PstI    PvuII
     SacI     ScaI    SexAI     SfcI     SmlI     SpeI     SspI     StuI
     StyI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AleI     AlfI     AloI     ApaI
    ApaLI     AscI    AsiSI    AvrII    BamHI     BbeI    BbvCI     BcgI
     BglI     BmtI     BplI    BsaBI    BsaXI    BsiEI    BsiWI    BspEI
    BspHI    BsrBI   BssHII    BstBI   Bsu36I    BtgZI     ClaI   DraIII
     DrdI     EagI     EciI    EcoNI EcoO109I     FseI     FspI    FspAI
     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PciI     PmeI    PpuMI    PshAI   PspOMI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SwaI    TaqII     XmaI     ZraI
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