Restriction map for pF1KE2407
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BcgI        BclI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaWI       BseAI       BsiWI       BsmBI       
BsoBI       BspEI       BssHII      Bst1107I    BstAPI      
BstEII      BstPI       BstZ17I     CelII       CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       KasI        KpnI        
MamI        MluI        MroI        NaeI        NarI        
NcoI        NgoMIV      NotI        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       PvuI        
RsrII       SbfI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AclI         3500  AACGTT               AA^CGTT
AflIII        453  ACATGT               A^C[GA][CT]GT
Alw44I       2693  GTGCAC               G^TGCAC
AlwNI        1583  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2461  CCTCAGG              CC^T[ACGT]AGG
ApaLI        2693  GTGCAC               G^TGCAC
BalI         3759  TGGCCA               TGG^CCA
BanII        2266  GAGCTC               G[GA]GC[CT]^C
BbrPI        2528  CACGTG               CAC^GTG
BbvCI        2743  CCTCAGC              CCTCAGC(-5/-2)
BciVI        2467  GTATCC               GTATCC(6/5)
BcnI         1848  CCGGG                CC^[GC]GG
BsiEI        2325  CGACCG               CG[GA][CT]^CG
BsmI         3345  GAATGC               GAATGC(1/-1)
Bsp1407I     2445  TGTACA               T^GTACA
BspDI         341  ATCGAT               AT^CGAT
BspHI        4200  TCATGA               T^CATGA
BspLU11I      453  ACATGT               A^CATGT
BspMI          50  ACCTGC               ACCTGC(4/8)
BsrBI        3178  GAGCGG               CCGCTC(-3/-3)
BsrFI        4548  ACCGGC               [GA]^CCGG[CT]
BsrGI        2445  TGTACA               T^GTACA
BssSI         659  CTCGTG               CACGAG(-5/-1)
BstBI        4410  TTCGAA               TT^CGAA
Bsu36I       2461  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       4548  ACCGGC               [GA]^CCGG[CT]
ClaI          341  ATCGAT               AT^CGAT
DraI         2161  TTTAAA               TTT^AAA
DraII        4289  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII       1406  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           44  CCGCGG               C^C[GA][CT]GG
Eco81I       2461  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     4289  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1901  GAATTC               G^AATTC
FauI           43  CCCGC                CCCGC(4/6)
HpaI          457  GTTAAC               GTT^AAC
KspI           44  CCGCGG               CCGC^GG
MfeI          551  CAATTG               C^AATTG
MluNI        3759  TGGCCA               TGG^CCA
MscI         3759  TGGCCA               TGG^CCA
MunI          551  CAATTG               C^AATTG
NciI         1848  CCGGG                CC^[GC]GG
NheI         2700  GCTAGC               G^CTAGC
NruI         2322  TCGCGA               TCG^CGA
NspV         4410  TTCGAA               TT^CGAA
PflMI         700  CCACAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2528  CACGTG               CAC^GTG
PmlI         2528  CACGTG               CAC^GTG
PpuMI        4289  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        2518  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3500  AACGTT               AA^CGTT
PstI         1512  CTGCAG               CTGCA^G
RcaI         4200  TCATGA               T^CATGA
SacI         2266  GAGCTC               GAGCT^C
SacII          44  CCGCGG               CCGC^GG
SalI         3488  GTCGAC               G^TCGAC
SapI         3740  GCTCTTC              GCTCTTC(1/4)
ScaI         3085  AGTACT               AGT^ACT
SfuI         4410  TTCGAA               TT^CGAA
SspBI        2445  TGTACA               T^GTACA
SspI         4078  AATATT               AAT^ATT
Van91I        700  CCACAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1755  CCATGCTCTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          173  GTAGAC               GT^[AC][GT]AC
             3488  GTCGAC              
AflII        1219  CTTAAG               C^TTAAG
             1927  CTTAAG              
AhdI         4161  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4505  GACTCCACGTC         
Asp700I      2777  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4172  GAAGGAGTTC          
AspEI        4161  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4505  GACTCCACGTC         
BfrI         1219  CTTAAG               C^TTAAG
             1927  CTTAAG              
BsaAI        1142  TACGTG               [CT]AC^GT[GA]
             2528  CACGTG              
BsaI          831  GAGACC               GGTCTC(1/5)
             3528  GAGACC              
BsiYI         700  CCACAAGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             3685  CCCAAACGAGG         
BslI          700  CCACAAGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             3685  CCCAAACGAGG         
BsmFI        4016  GTCCC                GGGAC(10/14)
             4291  GTCCC               
CfoI          297  GCGC                 GCG^C
             3349  GCGC                
EaeI         3759  TGGCCA               [CT]^GGCC[GA]
             4182  TGGCCG              
Eam1105I     4161  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4505  GACTCCACGTC         
EcoRV         319  GATATC               GAT^ATC
             1619  GATATC              
EcoT22I      1307  ATGCAT               ATGCA^T
             2789  ATGCAT              
HhaI          297  GCGC                 GCG^C
             3349  GCGC                
HinP1I        297  GCGC                 G^CGC
             3349  GCGC                
HincII        457  GTTAAC               GT[CT]^[GA]AC
             3488  GTCGAC              
HindII        457  GTTAAC               GT[CT]^[GA]AC
             3488  GTCGAC              
HindIII       673  AAGCTT               A^AGCTT
             4498  AAGCTT              
NdeI          329  CATATG               CA^TATG
             2792  CATATG              
NlaIV        2890  GGAGCC               GG[ACGT]^[ACGT]CC
             4290  GGTCCC              
NsiI         1307  ATGCAT               ATGCA^T
             2789  ATGCAT              
XmnI         2777  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4172  GAAGGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BbsI        
BbvI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsmAI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XhoII       
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