Restriction map for pF1KE2407
 (Linear) (Six-base) MAPSORT of: KIEE2407.seq  Check: 5563  from: 1  to: 4572



KIEE2407.orf

 With 182 enzymes: SgfI * 

                        May 11, 2012 13:46  ..

AccI GT'mk_AC

Cuts at:      0     174    3489    4572 
   Size:        174    3315    1083

  Fragments arranged by size: 

                3315    1083     174
 
AclI AA'CG_TT

Cuts at:      0    3501    4572 
   Size:       3501    1071
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     703     789    1478    1565    2117    2423    2735 
   Size:        217     486      86     689      87     552     306     312

Cuts at:   2735    3482    4017    4572 
   Size:        747     535     555

  Fragments arranged by size: 

                 747     689     555     552     535     486     312     306
                 217      87      86
 
AflII C'TTAA_G

Cuts at:      0    1219    1927    4572 
   Size:       1219     708    2645

  Fragments arranged by size: 

                2645    1219     708
 
AflIII A'CryG_T

Cuts at:      0     453    4572 
   Size:        453    4119
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4166    4510    4572 
   Size:       4166     344      62

  Fragments arranged by size: 

                4166     344      62
 
AleI CACnn'nnGTG

Cuts at:      0    1714    4572 
   Size:       1714    2858
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1588    4572 
   Size:       1588    2984
 
ApaLI G'TGCA_C

Cuts at:      0    2693    4572 
   Size:       2693    1879
 
ApoI r'AATT_y

Cuts at:      0     308    1901    1989    2167    2722    4572 
   Size:        308    1593      88     178     555    1850

  Fragments arranged by size: 

                1850    1593     555     308     178      88
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     172     205    4572 
   Size:        172      33    4367

  Fragments arranged by size: 

                4367     172      33
 
BanII G_rGCy'C

Cuts at:      0    2270    4572 
   Size:       2270    2302
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2411    3008    3516    3566    3702    4572 
   Size:       2411     597     508      50     136     870

  Fragments arranged by size: 

                2411     870     597     508     136      50
 
BbvCI CC'TCA_GC

Cuts at:      0    2744    4572 
   Size:       2744    1828
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2478    4572 
   Size:       2478    2094
 
BfrBI ATG'CAT

Cuts at:      0    1309    2791    4572 
   Size:       1309    1482    1781

  Fragments arranged by size: 

                1781    1482    1309
 
BglII A'GATC_T

Cuts at:      0      95    2779    4069    4572 
   Size:         95    2684    1290     503

  Fragments arranged by size: 

                2684    1290     503      95
 
Bme1580I G_kGCm'C

Cuts at:      0    2697    4572 
   Size:       2697    1875
 
BmgBI CAC'GTC

Cuts at:      0    4512    4572 
   Size:       4512      60
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1679    4174    4514    4572 
   Size:       1679    2495     340      58

  Fragments arranged by size: 

                2495    1679     340      58
 
BmtI G_CTAG'C

Cuts at:      0    2704    4572 
   Size:       2704    1868
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3275    3277    4572 
   Size:       3275       2    1295

  Fragments arranged by size: 

                3275    1295       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2501    2940    4127    4572 
   Size:       2501     439    1187     445

  Fragments arranged by size: 

                2501    1187     445     439
 
Bpu10I CC'TnA_GC

Cuts at:      0    2744    4572 
   Size:       2744    1828
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223    2438    3433    3644    3735    4572 
   Size:        223    2215     995     211      91     837

  Fragments arranged by size: 

                2215     995     837     223     211      91
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     825    3522    4572 
   Size:        825    2697    1050

  Fragments arranged by size: 

                2697    1050     825
 
BsaAI yAC'GTr

Cuts at:      0    1144    2530    4572 
   Size:       1144    1386    2042

  Fragments arranged by size: 

                2042    1386    1144
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3063    3093    3613    3643    4082    4112    4572 
   Size:       3063      30     520      30     439      30     460

  Fragments arranged by size: 

                3063     520     460     439      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1646    3507    3634    3857    4208    4572 
   Size:       1646    1861     127     223     351     364

  Fragments arranged by size: 

                1861    1646     364     351     223     127
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1527    1552    2563    2834    4572 
   Size:       1527      25    1011     271    1738

  Fragments arranged by size: 

                1738    1527    1011     271      25
 
BsiEI CG_ry'CG

Cuts at:      0    2328    4572 
   Size:       2328    2244
 
BsiHKAI G_wGCw'C

Cuts at:      0    2270    2697    3808    4572 
   Size:       2270     427    1111     764

  Fragments arranged by size: 

                2270    1111     764     427
 
BsmI GAATG_Cn'

Cuts at:      0    3351    4572 
   Size:       3351    1221
 
Bsp1286I G_dGCh'C

Cuts at:      0    2270    2697    3808    4572 
   Size:       2270     427    1111     764

  Fragments arranged by size: 

                2270    1111     764     427
 
BspHI T'CATG_A

Cuts at:      0    4200    4572 
   Size:       4200     372
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      59    4572 
   Size:         59    4513
 
BsrBI CCG'CTC

Cuts at:      0    3180    4572 
   Size:       3180    1392
 
BsrDI GCAATG_nn'

Cuts at:      0    3152    4040    4322    4572 
   Size:       3152     888     282     250

  Fragments arranged by size: 

                3152     888     282     250
 
BsrFI r'CCGG_y

Cuts at:      0    4548    4572 
   Size:       4548      24
 
BsrGI T'GTAC_A

Cuts at:      0    2445    4572 
   Size:       2445    2127
 
BssSI C'ACGA_G

Cuts at:      0     659    4572 
   Size:        659    3913
 
BstBI TT'CG_AA

Cuts at:      0    4411    4572 
   Size:       4411     161
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2576    3922    4527    4572 
   Size:       2576    1346     605      45

  Fragments arranged by size: 

                2576    1346     605      45
 
BstYI r'GATC_y

Cuts at:      0      95    1530    2457    2779    4069    4572 
   Size:         95    1435     927     322    1290     503

  Fragments arranged by size: 

                1435    1290     927     503     322      95
 
Bsu36I CC'TnA_GG

Cuts at:      0    2462    4572 
   Size:       2462    2110
 
BtgI C'CryG_G

Cuts at:      0      44    4572 
   Size:         44    4528
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3392    3611    4136    4572 
   Size:       3392     219     525     436

  Fragments arranged by size: 

                3392     525     436     219
 
BtsI GCAGTG_nn'

Cuts at:      0     403    3132    3928    4572 
   Size:        403    2729     796     644

  Fragments arranged by size: 

                2729     796     644     403
 
ClaI AT'CG_AT

Cuts at:      0     342    4572 
   Size:        342    4230
 
DraI TTT'AAA

Cuts at:      0    2163    4572 
   Size:       2163    2409
 
DraIII CAC_nnn'GTG

Cuts at:      0    1411    4572 
   Size:       1411    3161
 
EaeI y'GGCC_r

Cuts at:      0    3759    4182    4572 
   Size:       3759     423     390

  Fragments arranged by size: 

                3759     423     390
 
EarI CTCTTCn'nnn_

Cuts at:      0     819    1067    1460    2375    2711    3005    3620    3659 
   Size:        819     248     393     915     336     294     615      39

Cuts at:   3659    3747    4572 
   Size:         88     825

  Fragments arranged by size: 

                 915     825     819     615     393     336     294     248
                  88      39
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      91    4572 
   Size:         91    4481
 
EcoICRI GAG'CTC

Cuts at:      0    2268    4572 
   Size:       2268    2304
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     703     789    1478    1565    2117    2423    2501 
   Size:        217     486      86     689      87     552     306      78

Cuts at:   2501    2735    2940    3482    4017    4127    4572 
   Size:        234     205     542     535     110     445

  Fragments arranged by size: 

                 689     552     542     535     486     445     306     234
                 217     205     110      87      86      78
 
EcoO109I rG'GnC_Cy

Cuts at:      0    4290    4572 
   Size:       4290     282
 
EcoRI G'AATT_C

Cuts at:      0    1901    4572 
   Size:       1901    2671
 
EcoRV GAT'ATC

Cuts at:      0     321    1621    4572 
   Size:        321    1300    2951

  Fragments arranged by size: 

                2951    1300     321
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     539    1123    1942    3073    3217    3310    3803    4572 
   Size:        539     584     819    1131     144      93     493     769

  Fragments arranged by size: 

                1131     819     769     584     539     493     144      93
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     335     367    1556    1582    1588    1614    2101    2133 
   Size:        335      32    1189      26       6      26     487      32

Cuts at:   2133    3431    3463    4096    4128    4152    4184    4496    4528 
   Size:       1298      32     633      32      24      32     312      32

Cuts at:   4528    4572 
   Size:         44

  Fragments arranged by size: 

                1298    1189     633     487     335     312      44      32
                  32      32      32      32      32      26      26      24
                   6
 
HincII GTy'rAC

Cuts at:      0     459    3490    4572 
   Size:        459    3031    1082

  Fragments arranged by size: 

                3031    1082     459
 
HindIII A'AGCT_T

Cuts at:      0     673    4498    4572 
   Size:        673    3825      74

  Fragments arranged by size: 

                3825     673      74
 
HpaI GTT'AAC

Cuts at:      0     459    4572 
   Size:        459    4113
 
MfeI C'AATT_G

Cuts at:      0     551    4572 
   Size:        551    4021
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2534    3229    4572 
   Size:       2534     695    1343

  Fragments arranged by size: 

                2534    1343     695
 
MscI TGG'CCA

Cuts at:      0    3761    4572 
   Size:       3761     811
 
MslI CAynn'nnrTG

Cuts at:      0     110     434    1714    3920    4572 
   Size:        110     324    1280    2206     652

  Fragments arranged by size: 

                2206    1280     652     324     110
 
MspA1I CmG'CkG

Cuts at:      0       6      46    1894    2547    4256    4572 
   Size:          6      40    1848     653    1709     316

  Fragments arranged by size: 

                1848    1709     653     316      40       6
 
NdeI CA'TA_TG

Cuts at:      0     330    2793    4572 
   Size:        330    2463    1779

  Fragments arranged by size: 

                2463    1779     330
 
NheI G'CTAG_C

Cuts at:      0    2700    4572 
   Size:       2700    1872
 
NruI TCG'CGA

Cuts at:      0    2324    4572 
   Size:       2324    2248
 
NsiI A_TGCA'T

Cuts at:      0    1311    2793    4572 
   Size:       1311    1482    1779

  Fragments arranged by size: 

                1779    1482    1311
 
NspI r_CATG'y

Cuts at:      0     457    2334    2412    4572 
   Size:        457    1877      78    2160

  Fragments arranged by size: 

                2160    1877     457      78
 
PciI A'CATG_T

Cuts at:      0     453    4572 
   Size:        453    4119
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     706    4572 
   Size:        706    3866
 
PfoI T'CCnGG_A

Cuts at:      0    1847    2364    4572 
   Size:       1847     517    2208

  Fragments arranged by size: 

                2208    1847     517
 
PmlI CAC'GTG

Cuts at:      0    2530    4572 
   Size:       2530    2042
 
PpuMI rG'GwC_Cy

Cuts at:      0    4290    4572 
   Size:       4290     282
 
PshAI GACnn'nnGTC

Cuts at:      0    2522    4572 
   Size:       2522    2050
 
PsiI TTA'TAA

Cuts at:      0    3230    4572 
   Size:       3230    1342
 
PstI C_TGCA'G

Cuts at:      0    1516    4572 
   Size:       1516    3056
 
PvuII CAG'CTG

Cuts at:      0    1894    2547    4256    4572 
   Size:       1894     653    1709     316

  Fragments arranged by size: 

                1894    1709     653     316
 
SacI G_AGCT'C

Cuts at:      0    2270    4572 
   Size:       2270    2302
 
SacII CC_GC'GG

Cuts at:      0      47    4572 
   Size:         47    4525
 
SalI G'TCGA_C

Cuts at:      0    3488    4572 
   Size:       3488    1084
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3747    4572 
   Size:       3747     825
 
ScaI AGT'ACT

Cuts at:      0    3087    4572 
   Size:       3087    1485
 
SfcI C'TryA_G

Cuts at:      0    1512    1646    3872    4572 
   Size:       1512     134    2226     700

  Fragments arranged by size: 

                2226    1512     700     134
 
SmlI C'TyrA_G

Cuts at:      0     202    1219    1927    2453    3448    3659    3750    4572 
   Size:        202    1017     708     526     995     211      91     822

  Fragments arranged by size: 

                1017     995     822     708     526     211     202      91
            
 
SspI AAT'ATT

Cuts at:      0    4080    4572 
   Size:       4080     492
 
StyI C'CwwG_G

Cuts at:      0    2315    2520    2877    3308    3536    4572 
   Size:       2315     205     357     431     228    1036

  Fragments arranged by size: 

                2315    1036     431     357     228     205
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     807    3825    4533    4572 
   Size:        807    3018     708      39

  Fragments arranged by size: 

                3018     807     708      39
 
TatI w'GTAC_w

Cuts at:      0    2445    3085    3106    4572 
   Size:       2445     640      21    1466

  Fragments arranged by size: 

                2445    1466     640      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     369     401    4572 
   Size:        369      32    4171

  Fragments arranged by size: 

                4171     369      32
 
XbaI T'CTAG_A

Cuts at:      0    2632    3505    3520    4572 
   Size:       2632     873      15    1052

  Fragments arranged by size: 

                2632    1052     873      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1762    4572 
   Size:       1762    2810
 
XmnI GAAnn'nnTTC

Cuts at:      0    2781    4176    4572 
   Size:       2781    1395     396

  Fragments arranged by size: 

                2781    1395     396
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII   AflIII     AhdI     AleI    AlwNI
    ApaLI     ApoI     BaeI    BanII     BbsI    BbvCI    BciVI    BfrBI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DraI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     HpaI
     MfeI     MmeI     MscI     MslI   MspA1I     NdeI     NheI     NruI
     NsiI     NspI     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI     PstI    PvuII     SacI    SacII     SalI     SapI     ScaI
     SfcI     SmlI     SspI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI     AgeI     AlfI     AloI     ApaI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI
     BcgI     BclI     BglI     BlpI    BsaBI    BsaHI    BsaWI    BseYI
    BsiWI    BsmBI    BspEI   BssHII   BstAPI   BstEII  BstZ17I    CspCI
     DrdI     EagI    EcoNI     FseI     FspI    FspAI    HaeII     KasI
     KpnI     MluI     NaeI     NarI     NcoI   NgoMIV     NotI     PacI
     PasI     PmeI     PpiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XhoI
     XmaI     ZraI
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