################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AclI AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AvrII BbeI BbrPI BciVI BlnI BsaAI BsiEI BsiWI BspDI BspHI BssHII Bst1107I BstBI BstZ17I ClaI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FseI HpaI KasI KpnI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI SalI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 3694 GACGTC GACGT^C AccI 1048 GTCTAC GT^[AC][GT]AC AccIII 710 TCCGGA T^CCGGA AcsI 739 AAATTC [GA]^AATT[CT] AflII 3244 CTTAAG C^TTAAG AflIII 1478 ACATGT A^C[GA][CT]GT ApaI 4685 GGGCCC GGGCC^C ApoI 739 AAATTC [GA]^AATT[CT] AseI 3265 ATTAAT AT^TAAT AsnI 3265 ATTAAT AT^TAAT BfrI 3244 CTTAAG C^TTAAG BglII 1400 AGATCT A^GATCT BsaBI 4878 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 710 TCCGGA [AT]^CCGG[AT] BseAI 710 TCCGGA T^CCGGA BsmI 5154 GCATTC GAATGC(1/-1) Bsp1407I 398 TGTACA T^GTACA BspEI 710 TCCGGA T^CCGGA BspLU11I 1478 ACATGT A^CATGT BsrGI 398 TGTACA T^GTACA BstXI 4901 CCAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 4985 CGGTCCG CG^G[AT]CCG MamI 4878 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 710 TCCGGA T^CCGGA NcoI 1583 CCATGG C^CATGG PshBI 3265 ATTAAT AT^TAAT RsrII 4985 CGGTCCG CG^G[AT]CCG SbfI 1551 CCTGCAGG CCTGCA^GG Sse8387I 1551 CCTGCAGG CCTGCA^GG SspBI 398 TGTACA T^GTACA XbaI 69 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 1302 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2124 GAAGGGCTTC AspI 1941 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3049 GACAAGGTC BbvCI 4434 CCTCAGC CCTCAGC(-5/-2) 2707 GCTGAGG BclI 2259 TGATCA T^GATCA 2729 TGATCA BstEII 604 GGTGACC G^GT[ACGT]ACC 909 GGTCACC BstPI 604 GGTGACC G^GT[ACGT]ACC 909 GGTCACC DraIII 102 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 2008 CACTTTGTG DrdI 4978 GACGATGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5088 GACAGAGCGGTC EcoO65I 604 GGTGACC G^GT[ACGT]ACC 909 GGTCACC FbaI 2259 TGATCA T^GATCA 2729 TGATCA HindIII 3241 AAGCTT A^AGCTT 5761 AAGCTT KspI 296 CCGCGG CCGC^GG 3572 CCGCGG NaeI 1750 GCCGGC GCC^GGC 3463 GCCGGC NgoMIV 1750 GCCGGC G^CCGGC 3463 GCCGGC PflFI 1941 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3049 GACAAGGTC PflMI 3933 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4776 CCAGCGGGTGG SacII 296 CCGCGG CCGC^GG 3572 CCGCGG ScaI 578 AGTACT AGT^ACT 1364 AGTACT SexAI 221 ACCAGGT A^CC[AT]GGT 5603 ACCTGGT SfiI 3462 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5268 GGCCAAGAAGGCC SphI 2366 GCATGC GCATG^C 2910 GCATGC Tth111I 1941 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3049 GACAAGGTC Van91I 3933 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4776 CCAGCGGGTGG XmnI 1302 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2124 GAAGGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI AspHI AvaI AvaII AviII BalI BamHI BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI