Restriction map for
pF1KE2495
(Circular) (Six-base) MAPSORT of: pF1KE2495.seq Check: 6052 from: 1 to: 8930
pF1KE2495 8930 bp
With 182 enzymes: SgfI *
February 14, 2014 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1356 3192 3396 1356
Size: 1836 204 6890
Fragments arranged by size:
6890 1836 204
AatII G_ACGT'C
Cuts at: 3806 3806
Size: 8930
AccI GT'mk_AC
Cuts at: 1157 5968 1157
Size: 4811 4119
Acc65I G'GTAC_C
Cuts at: 5946 5946
Size: 8930
AclI AA'CG_TT
Cuts at: 8770 8770
Size: 8930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1121 2653 3127 3310 3457 4612 5083 5134 5440
Size: 1532 474 183 147 1155 471 51 306
Cuts at: 5440 5752 6755 7187 7934 8202 1121
Size: 312 1003 432 747 268 1849
Fragments arranged by size:
1849 1532 1155 1003 747 474 471 432
312 306 268 183 147 51
AfeI AGC'GCT
Cuts at: 430 2824 4414 430
Size: 2394 1590 4946
Fragments arranged by size:
4946 2394 1590
AflII C'TTAA_G
Cuts at: 3352 3352
Size: 8930
AflIII A'CryG_T
Cuts at: 1586 6282 7386 8363 1586
Size: 4696 1104 977 2153
Fragments arranged by size:
4696 2153 1104 977
AgeI A'CCGG_T
Cuts at: 7318 7318
Size: 8930
AleI CACnn'nnGTG
Cuts at: 2398 3586 2398
Size: 1188 7742
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1675 2221 5167 1675
Size: 546 2946 5438
Fragments arranged by size:
5438 2946 546
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 814 846 1711 1743 814
Size: 32 865 32 8001
Fragments arranged by size:
8001 865 32 32
AlwNI CAG_nnn'CTG
Cuts at: 978 3132 3411 3762 4302 5229 5259 5556 5712
Size: 2154 279 351 540 927 30 297 156
Cuts at: 5712 6235 7802 978
Size: 523 1567 2106
Fragments arranged by size:
2154 2106 1567 927 540 523 351 297
279 156 30
ApaI G_GGCC'C
Cuts at: 4797 4797
Size: 8930
ApaLI G'TGCA_C
Cuts at: 7700 7700
Size: 8930
ApoI r'AATT_y
Cuts at: 847 5934 8283 8539 847
Size: 5087 2349 256 1238
Fragments arranged by size:
5087 2349 1238 256
AseI AT'TA_AT
Cuts at: 20 3374 20
Size: 3354 5576
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8930
AvaI C'yCGr_G
Cuts at: 266 459 923 1316 2489 3533 4019 5950 266
Size: 193 464 393 1173 1044 486 1931 3246
Fragments arranged by size:
3246 1931 1173 1044 486 464 393 193
BamHI G'GATC_C
Cuts at: 857 1905 3220 5955 857
Size: 1048 1315 2735 3832
Fragments arranged by size:
3832 2735 1315 1048
BanI G'GyrC_C
Cuts at: 751 1240 5896 5946 6360 6608 6643 751
Size: 489 4656 50 414 248 35 3038
Fragments arranged by size:
4656 3038 489 414 248 50 35
BanII G_rGCy'C
Cuts at: 404 1097 1389 2038 2921 2963 4271 4556 4797
Size: 693 292 649 883 42 1308 285 241
Cuts at: 4797 5663 5944 6974 404
Size: 866 281 1030 2360
Fragments arranged by size:
2360 1308 1030 883 866 693 649 292
285 281 241 42
BbeI G_GCGC'C
Cuts at: 6364 6612 6364
Size: 248 8682
BbsI GAAGACnn'nnnn_
Cuts at: 313 664 2951 3161 4366 5687 313
Size: 351 2287 210 1205 1321 3556
Fragments arranged by size:
3556 2287 1321 1205 351 210
BbvCI CC'TCA_GC
Cuts at: 2816 4543 2816
Size: 1727 7203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2260 2294 3658 3692 4097 4131 2260
Size: 34 1364 34 405 34 7059
Fragments arranged by size:
7059 1364 405 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6828 7589 8930 6828
Size: 761 1341 6828
Fragments arranged by size:
6828 1341 761
BclI T'GATC_A
Cuts at: 2367 2837 6450 2367
Size: 470 3613 4847
Fragments arranged by size:
4847 3613 470
BglI GCCn_nnn'nGGC
Cuts at: 124 3577 4318 4327 5383 5407 5764 124
Size: 3453 741 9 1056 24 357 3290
Fragments arranged by size:
3453 3290 1056 741 357 24 9
BglII A'GATC_T
Cuts at: 1508 6445 8482 1508
Size: 4937 2037 1956
Fragments arranged by size:
4937 2037 1956
BlpI GC'TnA_GC
Cuts at: 2350 2455 3083 4999 5162 6042 2350
Size: 105 628 1916 163 880 5238
Fragments arranged by size:
5238 1916 880 628 163 105
Bme1580I G_kGCm'C
Cuts at: 227 754 4797 6555 6648 7704 227
Size: 527 4043 1758 93 1056 1453
Fragments arranged by size:
4043 1758 1453 1056 527 93
BmgBI CAC'GTC
Cuts at: 1122 3584 1122
Size: 2462 6468
BmrI ACTGGGnnnn_n'
Cuts at: 141 2006 2287 4215 6142 6223 6558 8702 141
Size: 1865 281 1928 1927 81 335 2144 369
Fragments arranged by size:
2144 1928 1927 1865 369 335 281 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4554 4656 7297 4554
Size: 102 2641 6187
Fragments arranged by size:
6187 2641 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 811 934 1471 1618 1732 2138 2230 2452
Size: 33 123 537 147 114 406 92 222
Cuts at: 2452 2722 2752 2878 2911 3004 3214 3235 3277
Size: 270 30 126 33 93 210 21 42
Cuts at: 3277 3298 3487 3499 3562 3667 3784 3811 3868
Size: 21 189 12 63 105 117 27 57
Cuts at: 3868 3889 4090 4375 4534 4609 4711 4732 4747
Size: 21 201 285 159 75 102 21 15
Cuts at: 4747 4765 4786 5035 5188 5308 5470 5599 5611
Size: 18 21 249 153 120 162 129 12
Cuts at: 5611 778
Size: 4097
Fragments arranged by size:
4097 537 406 285 270 249 222 210
201 189 162 159 153 147 129 126
123 120 117 114 105 102 93 92
75 63 57 42 33 33 30 27
21 21 21 21 21 18 15 12
12
Bpu10I CC'TnA_GC
Cuts at: 2816 3503 4543 2816
Size: 687 1040 7203
Fragments arranged by size:
7203 1040 687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1282 1936 2947 4549 5512 6106 6985 7477
Size: 876 654 1011 1602 963 594 879 492
Cuts at: 7477 7775 406
Size: 298 1561
Fragments arranged by size:
1602 1561 1011 963 879 876 654 594
492 298
BsaI GGTCTCn'nnnn_
Cuts at: 895 1447 4594 4967 5230 8634 895
Size: 552 3147 373 263 3404 1191
Fragments arranged by size:
3404 3147 1191 552 373 263
BsaAI yAC'GTr
Cuts at: 6283 6283
Size: 8930
BsaBI GATnn'nnATC
Cuts at: 6 4990 6444 8481 6
Size: 4984 1454 2037 455
Fragments arranged by size:
4984 2037 1454 455
BsaHI Gr'CG_yC
Cuts at: 1154 3803 4247 6361 6609 7311 8807 1154
Size: 2649 444 2114 248 702 1496 1277
Fragments arranged by size:
2649 2114 1496 1277 702 444 248
BsaWI w'CCGG_w
Cuts at: 818 6119 6640 7318 7592 7739 8150 818
Size: 5301 521 678 274 147 411 1598
Fragments arranged by size:
5301 1598 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 266 296 556 586 607 637 814 817 844
Size: 30 260 30 21 30 177 3 27
Cuts at: 844 847 1705 1711 1735 1741 3793 3823 3841
Size: 3 858 6 24 6 2052 30 18
Cuts at: 3841 3871 4489 4519 4720 4750 266
Size: 30 618 30 201 30 4446
Fragments arranged by size:
4446 2052 858 618 260 201 177 30
30 30 30 30 30 30 27 24
21 18 6 6 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 561 822 1105 1156 1234 1621 1729 1732 1965
Size: 261 283 51 78 387 108 3 233
Cuts at: 1965 2449 2749 2833 2914 2917 3067 3475 3484
Size: 484 300 84 81 3 150 408 9
Cuts at: 3484 3487 3559 3664 4087 4090 4171 4174 4279
Size: 3 72 105 423 3 81 3 105
Cuts at: 4279 4330 4338 4510 4594 4762 4789 4870 5185
Size: 51 8 172 84 168 27 81 315
Cuts at: 5185 5353 5383 5428 5776 5785 5848 5935 561
Size: 168 30 45 348 9 63 87 3556
Fragments arranged by size:
3556 484 423 408 387 348 315 300
283 261 233 172 168 168 150 108
105 105 87 84 84 81 81 81
78 72 63 51 51 45 30 27
9 9 8 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1125 3061 3720 3938 4040 4295 4796 5318 6355
Size: 1936 659 218 102 255 501 522 1037
Cuts at: 6355 7113 7690 8186 1125
Size: 758 577 496 1869
Fragments arranged by size:
1936 1869 1037 758 659 577 522 501
496 255 218 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 1381 2806 3610 5272 5368 5376 5830 379
Size: 1002 1425 804 1662 96 8 454 3479
Fragments arranged by size:
3479 1662 1425 1002 804 454 96 8
BsiEI CG_ry'CG
Cuts at: 104 6472 6518 7726 104
Size: 6368 46 1208 1308
Fragments arranged by size:
6368 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1097 2921 2956 2963 4271 4348 4556 4595 4772
Size: 1824 35 7 1308 77 208 39 177
Cuts at: 4772 5663 5944 6722 6912 7704 1097
Size: 891 281 778 190 792 2323
Fragments arranged by size:
2323 1824 1308 891 792 778 281 208
190 177 77 39 35 7
BsmI GAATG_Cn'
Cuts at: 5262 5262
Size: 8930
BsmBI CGTCTCn'nnnn_
Cuts at: 2638 2689 4234 2638
Size: 51 1545 7334
Fragments arranged by size:
7334 1545 51
Bsp1286I G_dGCh'C
Cuts at: 227 404 754 1097 1389 2038 2921 2956 2963
Size: 177 350 343 292 649 883 35 7
Cuts at: 2963 4271 4348 4556 4595 4772 4797 5663 5944
Size: 1308 77 208 39 177 25 866 281
Cuts at: 5944 6555 6648 6722 6912 6974 7704 227
Size: 611 93 74 190 62 730 1453
Fragments arranged by size:
1453 1308 883 866 730 649 611 350
343 292 281 208 190 177 177 93
77 74 62 39 35 25 7
BspEI T'CCGG_A
Cuts at: 818 8150 818
Size: 7332 1598
BspHI T'CATG_A
Cuts at: 8106 8106
Size: 8930
BspMI ACCTGCnnnn'nnnn_
Cuts at: 917 1301 1356 1667 2780 3192 3396 3749 4145
Size: 384 55 311 1113 412 204 353 396
Cuts at: 4145 5159 5498 5702 5980 6194 6496 6877 917
Size: 1014 339 204 278 214 302 381 2970
Fragments arranged by size:
2970 1113 1014 412 396 384 381 353
339 311 302 278 214 204 204 55
BsrBI CCG'CTC
Cuts at: 50 787 2145 3558 4833 5139 5202 5247 5646
Size: 737 1358 1413 1275 306 63 45 399
Cuts at: 5646 5838 7222 7276 8760 8925 50
Size: 192 1384 54 1484 165 55
Fragments arranged by size:
1484 1413 1384 1358 1275 737 399 306
192 165 63 55 54 45
BsrDI GCAATG_nn'
Cuts at: 284 698 810 2205 6842 284
Size: 414 112 1395 4637 2372
Fragments arranged by size:
4637 2372 1395 414 112
BsrFI r'CCGG_y
Cuts at: 501 1858 3571 4370 5498 6928 7109 7318 501
Size: 1357 1713 799 1128 1430 181 209 2113
Fragments arranged by size:
2113 1713 1430 1357 1128 799 209 181
BsrGI T'GTAC_A
Cuts at: 506 506
Size: 8930
BssSI C'ACGA_G
Cuts at: 397 3667 4771 7201 7559 397
Size: 3270 1104 2430 358 1768
Fragments arranged by size:
3270 2430 1768 1104 358
BstAPI GCAn_nnn'nTGC
Cuts at: 215 1359 2190 5259 5910 6307 215
Size: 1144 831 3069 651 397 2838
Fragments arranged by size:
3069 2838 1144 831 651 397
BstBI TT'CG_AA
Cuts at: 7291 7291
Size: 8930
BstEII G'GTnAC_C
Cuts at: 712 1017 712
Size: 305 8625
BstXI CCAn_nnnn'nTGG
Cuts at: 5016 5016
Size: 8930
BstYI r'GATC_y
Cuts at: 1 857 1508 1905 3220 4466 4784 4991 5075
Size: 856 651 397 1315 1246 318 207 84
Cuts at: 5075 5310 5955 6422 6445 6780 7026 8038 8124
Size: 235 645 467 23 335 246 1012 86
Cuts at: 8124 8136 8482 1
Size: 12 346 449
Fragments arranged by size:
1315 1246 1012 856 651 645 467 449
397 346 335 318 246 235 207 86
84 23 12
Bsu36I CC'TnA_GG
Cuts at: 2098 2306 4948 2098
Size: 208 2642 6080
Fragments arranged by size:
6080 2642 208
BtgI C'CryG_G
Cuts at: 404 717 1067 1691 3680 4322 5183 404
Size: 313 350 624 1989 642 861 4151
Fragments arranged by size:
4151 1989 861 642 624 350 313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1479 3063 5109 5832 6880 7061 7224 1479
Size: 1584 2046 723 1048 181 163 3185
Fragments arranged by size:
3185 2046 1584 1048 723 181 163
BtsI GCAGTG_nn'
Cuts at: 1406 2921 3674 4108 5351 6173 1406
Size: 1515 753 434 1243 822 4163
Fragments arranged by size:
4163 1515 1243 822 753 434
DraI TTT'AAA
Cuts at: 5929 5929
Size: 8930
DraIII CAC_nnn'GTG
Cuts at: 215 2121 215
Size: 1906 7024
DrdI GACnn_nn'nnGTC
Cuts at: 5092 5202 6636 7494 5092
Size: 110 1434 858 6528
Fragments arranged by size:
6528 1434 858 110
EaeI y'GGCC_r
Cuts at: 377 524 1100 1391 1961 2297 2388 2660 2996
Size: 147 576 291 570 336 91 272 336
Cuts at: 2996 3713 4472 6515 6689 7080 7107 8452 377
Size: 717 759 2043 174 391 27 1345 855
Fragments arranged by size:
2043 1345 855 759 717 576 570 391
336 336 291 272 174 147 91 27
EagI C'GGCC_G
Cuts at: 6515 6515
Size: 8930
EarI CTCTTCn'nnn_
Cuts at: 233 472 538 1975 2629 2669 3560 3769 3899
Size: 239 66 1437 654 40 891 209 130
Cuts at: 3899 4268 4561 5632 6953 7163 233
Size: 369 293 1071 1321 210 2000
Fragments arranged by size:
2000 1437 1321 1071 891 654 369 293
239 210 209 130 66 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1243 2806 5233 5782 5857 7138 7448 7594 8740
Size: 1563 2427 549 75 1281 310 146 1146
Cuts at: 8740 1243
Size: 1433
Fragments arranged by size:
2427 1563 1433 1281 1146 549 310 146
75
EcoICRI GAG'CTC
Cuts at: 1095 2919 2961 4269 4554 5661 5942 1095
Size: 1824 42 1308 285 1107 281 4083
Fragments arranged by size:
4083 1824 1308 1107 285 281 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 811 934 1121 1471 1618 1732 2138 2230
Size: 33 123 187 350 147 114 406 92
Cuts at: 2230 2452 2653 2722 2752 2878 2911 3004 3127
Size: 222 201 69 30 126 33 93 123
Cuts at: 3127 3214 3235 3277 3298 3310 3457 3487 3499
Size: 87 21 42 21 12 147 30 12
Cuts at: 3499 3562 3667 3784 3811 3868 3889 4090 4375
Size: 63 105 117 27 57 21 201 285
Cuts at: 4375 4534 4609 4612 4711 4732 4747 4765 4786
Size: 159 75 3 99 21 15 18 21
Cuts at: 4786 5035 5083 5134 5188 5308 5440 5470 5599
Size: 249 48 51 54 120 132 30 129
Cuts at: 5599 5611 5752 6755 7187 7934 8202 778
Size: 12 141 1003 432 747 268 1506
Fragments arranged by size:
1506 1003 747 432 406 350 285 268
249 222 201 201 187 159 147 147
141 132 129 126 123 123 120 117
114 105 99 93 92 87 75 69
63 57 54 51 48 42 33 33
30 30 30 27 21 21 21 21
21 18 15 12 12 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1297 1714 2755 3049 3649 4198 4630 4747 5155
Size: 417 1041 294 600 549 432 117 408
Cuts at: 5155 1297
Size: 5072
Fragments arranged by size:
5072 1041 600 549 432 417 408 294
117
EcoO109I rG'GnC_Cy
Cuts at: 555 3129 3642 4053 4944 5487 5709 6069 555
Size: 2574 513 411 891 543 222 360 3416
Fragments arranged by size:
3416 2574 891 543 513 411 360 222
EcoRI G'AATT_C
Cuts at: 5934 5934
Size: 8930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2421 2628 3367 6949 2421
Size: 207 739 3582 4402
Fragments arranged by size:
4402 3582 739 207
FspI TGC'GCA
Cuts at: 2506 3247 3691 6711 2506
Size: 741 444 3020 4725
Fragments arranged by size:
4725 3020 741 444
FspAI rTGC'GCAy
Cuts at: 2506 3247 2506
Size: 741 8189
HaeII r_GCGC'y
Cuts at: 432 2826 4003 4416 5444 5754 6364 6612 7634
Size: 2394 1177 413 1028 310 610 248 1022
Cuts at: 7634 8613 432
Size: 979 749
Fragments arranged by size:
2394 1177 1028 1022 979 749 610 413
310 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 266 298 607 639 817 849 2094 2126 2938
Size: 32 309 32 178 32 1245 32 812
Cuts at: 2938 2970 3793 3825 3841 3873 4489 4501 4521
Size: 32 823 32 16 32 616 12 20
Cuts at: 4521 4533 4720 4752 6772 6802 6804 6834 266
Size: 12 187 32 2020 30 2 30 2362
Fragments arranged by size:
2362 2020 1245 823 812 616 309 187
178 32 32 32 32 32 32 32
32 30 30 20 16 12 12 2
HincII GTy'rAC
Cuts at: 483 3165 5865 5969 483
Size: 2682 2700 104 3444
Fragments arranged by size:
3444 2700 2682 104
HindIII A'AGCT_T
Cuts at: 3349 5869 3349
Size: 2520 6410
KasI G'GCGC_C
Cuts at: 6360 6608 6360
Size: 248 8682
KpnI G_GTAC'C
Cuts at: 5950 5950
Size: 8930
MluI A'CGCG_T
Cuts at: 8363 8363
Size: 8930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 605 1007 1814 3836 5288 5309 7601 7785 605
Size: 402 807 2022 1452 21 2292 184 1750
Fragments arranged by size:
2292 2022 1750 1452 807 402 184 21
MscI TGG'CCA
Cuts at: 379 1102 1393 1963 2299 2390 2662 2998 6691
Size: 723 291 570 336 91 272 336 3693
Cuts at: 6691 379
Size: 2618
Fragments arranged by size:
3693 2618 723 570 336 336 291 272
91
MslI CAynn'nnrTG
Cuts at: 379 1591 1744 2398 2473 3586 6919 7046 379
Size: 1212 153 654 75 1113 3333 127 2263
Fragments arranged by size:
3333 2263 1212 1113 654 153 127 75
MspA1I CmG'CkG
Cuts at: 142 406 939 1215 1288 2190 2628 2835 2856
Size: 264 533 276 73 902 438 207 21
Cuts at: 2856 3339 3682 3747 4014 4068 4257 4299 4710
Size: 483 343 65 267 54 189 42 411
Cuts at: 4710 4887 5082 5256 5547 6041 6355 6715 7728
Size: 177 195 174 291 494 314 360 1013
Cuts at: 7728 7973 142
Size: 245 1099
Fragments arranged by size:
1099 1013 902 533 494 483 438 411
360 343 314 291 276 267 264 245
207 195 189 177 174 73 65 54
42 21
NaeI GCC'GGC
Cuts at: 1860 3573 7111 1860
Size: 1713 3538 3679
Fragments arranged by size:
3679 3538 1713
NarI GG'CG_CC
Cuts at: 6361 6609 6361
Size: 248 8682
NcoI C'CATG_G
Cuts at: 1691 1691
Size: 8930
NgoMIV G'CCGG_C
Cuts at: 1858 3571 7109 1858
Size: 1713 3538 3679
Fragments arranged by size:
3679 3538 1713
NspI r_CATG'y
Cuts at: 603 1011 1590 1728 2478 3022 5983 6483 7390
Size: 408 579 138 750 544 2961 500 907
Cuts at: 7390 603
Size: 2143
Fragments arranged by size:
2961 2143 907 750 579 544 500 408
138
PciI A'CATG_T
Cuts at: 1586 7386 1586
Size: 5800 3130
PflMI CCAn_nnn'nTGG
Cuts at: 4047 4890 4047
Size: 843 8087
PfoI T'CCnGG_A
Cuts at: 1487 1709 5206 1487
Size: 222 3497 5211
Fragments arranged by size:
5211 3497 222
PmeI GTTT'AAAC
Cuts at: 5929 5929
Size: 8930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 814 846 1399 1431 1711 1743 2299 2331 3607
Size: 32 553 32 280 32 556 32 1276
Cuts at: 3607 3639 8069 8101 814
Size: 32 4430 32 1643
Fragments arranged by size:
4430 1643 1276 556 553 280 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3642 4053 4944 5487 5709 3642
Size: 411 891 543 222 6863
Fragments arranged by size:
6863 891 543 411 222
PspOMI G'GGCC_C
Cuts at: 4793 4793
Size: 8930
PstI C_TGCA'G
Cuts at: 894 1568 1664 2230 2759 2777 3338 3665 3746
Size: 674 96 566 529 18 561 327 81
Cuts at: 3746 3956 4115 4187 4307 4434 4736 4907 5159
Size: 210 159 72 120 127 302 171 252
Cuts at: 5159 5495 5552 5977 6662 894
Size: 336 57 425 685 3162
Fragments arranged by size:
3162 685 674 566 561 529 425 336
327 302 252 210 171 159 127 120
96 81 72 57 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8930
PvuII CAG'CTG
Cuts at: 939 1288 2190 2835 2856 3339 4014 4068 4710
Size: 349 902 645 21 483 675 54 642
Cuts at: 4710 5082 5256 5547 6355 6715 939
Size: 372 174 291 808 360 3154
Fragments arranged by size:
3154 902 808 675 645 642 483 372
360 349 291 174 54 21
RsrII CG'GwC_CG
Cuts at: 5094 7125 5094
Size: 2031 6899
SacI G_AGCT'C
Cuts at: 1097 2921 2963 4271 4556 5663 5944 1097
Size: 1824 42 1308 285 1107 281 4083
Fragments arranged by size:
4083 1824 1308 1107 285 281 42
SacII CC_GC'GG
Cuts at: 407 3683 407
Size: 3276 5654
SalI G'TCGA_C
Cuts at: 5967 5967
Size: 8930
SapI GCTCTTCn'nnn_
Cuts at: 2629 3769 4561 5632 6953 7163 2629
Size: 1140 792 1071 1321 210 4396
Fragments arranged by size:
4396 1321 1140 1071 792 210
SbfI CC_TGCA'GG
Cuts at: 1664 5977 1664
Size: 4313 4617
ScaI AGT'ACT
Cuts at: 688 1474 8447 688
Size: 786 6973 1171
Fragments arranged by size:
6973 1171 786
SexAI A'CCwGG_T
Cuts at: 329 5711 329
Size: 5382 3548
SfcI C'TryA_G
Cuts at: 33 890 1564 1660 2226 2755 2773 3334 3424
Size: 857 674 96 566 529 18 561 90
Cuts at: 3424 3661 3742 3952 4111 4183 4303 4375 4430
Size: 237 81 210 159 72 120 72 55
Cuts at: 4430 4732 4903 5155 5491 5548 5704 5973 6658
Size: 302 171 252 336 57 156 269 685
Cuts at: 6658 7651 7842 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 857 685 674 566 561 529
336 302 269 252 237 210 191 171
159 156 120 96 90 81 72 72
57 55 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 3577 5383 3577
Size: 1806 7124
SfoI GGC'GCC
Cuts at: 6362 6610 6362
Size: 248 8682
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8930
SmaI CCC'GGG
Cuts at: 268 925 2491 4021 5952 268
Size: 657 1566 1530 1931 3246
Fragments arranged by size:
3246 1931 1566 1530 657
SmlI C'TyrA_G
Cuts at: 421 1297 1951 2962 3352 4564 5527 6085 7000
Size: 876 654 1011 390 1212 963 558 915
Cuts at: 7000 7492 7754 421
Size: 492 262 1597
Fragments arranged by size:
1597 1212 1011 963 915 876 654 558
492 390 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8930
SphI G_CATG'C
Cuts at: 2478 3022 5983 6483 2478
Size: 544 2961 500 4925
Fragments arranged by size:
4925 2961 544 500
StuI AGG'CCT
Cuts at: 796 1666 4759 5743 796
Size: 870 3093 984 3983
Fragments arranged by size:
3983 3093 984 870
StyI C'CwwG_G
Cuts at: 1346 1394 1691 1733 2396 2999 3092 3836 4172
Size: 48 297 42 663 603 93 744 336
Cuts at: 4172 4199 6064 6418 1346
Size: 27 1865 354 3858
Fragments arranged by size:
3858 1865 744 663 603 354 336 297
93 48 42 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 366 2122 3952 3973 5086 6513 6646 7105
Size: 239 1756 1830 21 1113 1427 133 459
Cuts at: 7105 7314 127
Size: 209 1743
Fragments arranged by size:
1830 1756 1743 1427 1113 459 239 209
133 21
TatI w'GTAC_w
Cuts at: 506 596 686 1370 1472 1610 2378 8445 506
Size: 90 90 684 102 138 768 6067 991
Fragments arranged by size:
6067 991 768 684 138 102 90 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 556 588 1011 1043 1705 1737 2935 2967 4664
Size: 32 423 32 662 32 1198 32 1697
Cuts at: 4664 4696 8447 8479 556
Size: 32 3751 32 1007
Fragments arranged by size:
3751 1697 1198 1007 662 423 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2052 3160 6727 2052
Size: 1108 3567 4255
Fragments arranged by size:
4255 3567 1108
XbaI T'CTAG_A
Cuts at: 177 5961 177
Size: 5784 3146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 786 879 1386 2383 3504 3843 4212 4287 5445
Size: 93 507 997 1121 339 369 75 1158
Cuts at: 5445 6425 786
Size: 980 3291
Fragments arranged by size:
3291 1158 1121 997 980 507 369 339
93 75
XmaI C'CCGG_G
Cuts at: 266 923 2489 4019 5950 266
Size: 657 1566 1530 1931 3246
Fragments arranged by size:
3246 1931 1566 1530 657
XmnI GAAnn'nnTTC
Cuts at: 1414 2236 1414
Size: 822 8108
ZraI GAC'GTC
Cuts at: 3804 3804
Size: 8930
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AhdI AscI AvrII BaeI BfrBI BmtI BsiWI BssHII
BstZ17I ClaI CspCI EcoRV FseI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspXI PsrI SanDI SgrAI SnaBI SrfI SspI
SwaI XhoI