Restriction map for pF1KE0900
 (Circular) (Six-base) MAPSORT of: pF1KE0900.seq  Check: 9672  from: 1  to: 13367



pF1KE0900  13367 bp

 With 182 enzymes: SgfI * 

                        December 21, 2016 11:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   5640    5640 
   Size:      13367
 
AccI GT'mk_AC

Cuts at:    444    3635    4971    7729    8473    9933   10405     444 
   Size:       3191    1336    2758     744    1460     472    3406

  Fragments arranged by size: 

                3406    3191    2758    1460    1336     744     472
 
Acc65I G'GTAC_C

Cuts at:  10383   10383 
   Size:      13367
 
AclI AA'CG_TT

Cuts at:   2597   13207    2597 
   Size:      10610    2757
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    200     847    1037    1196    1415    1727    2084    2765    3117 
   Size:        647     190     159     219     312     357     681     352

Cuts at:   3117    3821    5954    6518    7543    7820    9261    9689   11192 
   Size:        704    2133     564    1025     277    1441     428    1503

Cuts at:  11192   11624   12371   12639     200 
   Size:        432     747     268     928

  Fragments arranged by size: 

                2133    1503    1441    1025     928     747     704     681
                 647     564     432     428     357     352     312     277
                 268     219     190     159
 
AflII C'TTAA_G

Cuts at:   4953    4953 
   Size:      13367
 
AflIII A'CryG_T

Cuts at:    555    2131    6871    7611   10719   11823   12800     555 
   Size:       1576    4740     740    3108    1104     977    1122

  Fragments arranged by size: 

                4740    3108    1576    1122    1104     977     740
 
AgeI A'CCGG_T

Cuts at:   9222   11755    9222 
   Size:       2533   10834
 
AhdI GACnn_n'nnGTC

Cuts at:   3008    8348    3008 
   Size:       5340    8027
 
AleI CACnn'nnGTG

Cuts at:   9010    9010 
   Size:      13367
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   8810    8825    8810 
   Size:         15   13352
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    416     448    7682    7714     416 
   Size:         32    7234      32    6069

  Fragments arranged by size: 

                7234    6069      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   2224    2743    7507   10672   12239    2224 
   Size:        519    4764    3165    1567    3352

  Fragments arranged by size: 

                4764    3352    3165    1567     519
 
ApaLI G'TGCA_C

Cuts at:   8771   12137    8771 
   Size:       3366   10001
 
ApoI r'AATT_y

Cuts at:    146     406     513     921    1100    1437    1773    1815    3484 
   Size:        260     107     408     179     337     336      42    1669

Cuts at:   3484    4095    4111    4449    4494    4893    5103    5756    5891 
   Size:        611      16     338      45     399     210     653     135

Cuts at:   5891    6258    6352    6493    6903    6982    7149    7171    7903 
   Size:        367      94     141     410      79     167      22     732

Cuts at:   7903    7958    8029    8711    8722    9128    9236    9283   10059 
   Size:         55      71     682      11     406     108      47     776

Cuts at:  10059   10087   10097   10371   12720   12976     146 
   Size:         28      10     274    2349     256     537

  Fragments arranged by size: 

                2349    1669     776     732     682     653     611     537
                 410     408     406     399     367     338     337     336
                 274     260     256     210     179     167     141     135
                 108     107      94      79      71      55      47      45
                  42      28      22      16      11      10
 
AseI AT'TA_AT

Cuts at:     20    3302    7454      20 
   Size:       3282    4152    5933

  Fragments arranged by size: 

                5933    4152    3282
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:      13367
 
AvaI C'yCGr_G

Cuts at:  10387   10387 
   Size:      13367
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   7088    7121    7088 
   Size:         33   13334
 
BamHI G'GATC_C

Cuts at:    118     672    7938   10392     118 
   Size:        554    7266    2454    3093

  Fragments arranged by size: 

                7266    3093    2454     554
 
BanI G'GyrC_C

Cuts at:   4938   10021   10383   10797   11045   11080    4938 
   Size:       5083     362     414     248      35    7225

  Fragments arranged by size: 

                7225    5083     414     362     248      35
 
BanII G_rGCy'C

Cuts at:   1326    6935    7917    8389    9269   10381   11411    1326 
   Size:       5609     982     472     880    1112    1030    3282

  Fragments arranged by size: 

                5609    3282    1112    1030     982     880     472
 
BbeI G_GCGC'C

Cuts at:  10801   11049   10801 
   Size:        248   13119
 
BbsI GAAGACnn'nnnn_

Cuts at:    448    2772    6373    7549    8611    9752     448 
   Size:       2324    3601    1176    1062    1141    4063

  Fragments arranged by size: 

                4063    3601    2324    1176    1141    1062
 
BbvCI CC'TCA_GC

Cuts at:    665     665 
   Size:      13367
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124   10120   10154      90 
   Size:         34    9996      34    3303

  Fragments arranged by size: 

                9996    3303      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:  11265   12026   13367   11265 
   Size:        761    1341   11265

  Fragments arranged by size: 

               11265    1341     761
 
BclI T'GATC_A

Cuts at:   2391    4185    4458   10277   10887    2391 
   Size:       1794     273    5819     610    4871

  Fragments arranged by size: 

                5819    4871    1794     610     273
 
BfrBI ATG'CAT

Cuts at:   5503    7074    9651    5503 
   Size:       1571    2577    9219

  Fragments arranged by size: 

                9219    2577    1571
 
BglII A'GATC_T

Cuts at:  10882   12919   10882 
   Size:       2037   11330
 
BlpI GC'TnA_GC

Cuts at:  10479   10479 
   Size:      13367
 
Bme1580I G_kGCm'C

Cuts at:   8775   10992   11085   12141    8775 
   Size:       2217      93    1056   10001

  Fragments arranged by size: 

               10001    2217    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1956    2320    4588    8292   10579   10660   10995   13139    1956 
   Size:        364    2268    3704    2287      81     335    2144    2184

  Fragments arranged by size: 

                3704    2287    2268    2184    2144     364     335      81
            
 
BmtI G_CTAG'C

Cuts at:   4691    4691 
   Size:      13367
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3308   11734    3308 
   Size:       8426    4941
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1568    6197    7022    7913    9466    9548    1568 
   Size:       4629     825     891    1553      82    5387

  Fragments arranged by size: 

                5387    4629    1553     891     825      82
 
Bpu10I CC'TnA_GC

Cuts at:    665    4946    5112    8047     665 
   Size:       4281     166    2935    5985

  Fragments arranged by size: 

                5985    4281    2935     166
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4277    8878    9948   10543   11422   11914   12212    4277 
   Size:       4601    1070     595     879     492     298    5432

  Fragments arranged by size: 

                5432    4601    1070     879     595     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1298    4843   13071    1298 
   Size:       3545    8228    1594

  Fragments arranged by size: 

                8228    3545    1594
 
BsaAI yAC'GTr

Cuts at:   1450    6208    7733    8770   10720    1450 
   Size:       4758    1525    1037    1950    4097

  Fragments arranged by size: 

                4758    4097    1950    1525    1037
 
BsaBI GATnn'nnATC

Cuts at:      6    1196    3556    3593   10881   12918       6 
   Size:       1190    2360      37    7288    2037     455

  Fragments arranged by size: 

                7288    2360    2037    1190     455      37
 
BsaHI Gr'CG_yC

Cuts at:   9078   10798   11046   11748   13244    9078 
   Size:       1720     248     702    1496    9201

  Fragments arranged by size: 

                9201    1720    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   9222   10556   11077   11755   12029   12176   12587    9222 
   Size:       1334     521     678     274     147     411   10002

  Fragments arranged by size: 

               10002    1334     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    653     683    1240    1270    7682    7712    8534    8564     653 
   Size:         30     557      30    6412      30     822      30    5456

  Fragments arranged by size: 

                6412    5456     822     557      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   2980    6941   10055    2980 
   Size:       3961    3114    6292

  Fragments arranged by size: 

                6292    3961    3114
 
BseYI C'CCAG_C

Cuts at:   8847   10792   11550   12127   12623    8847 
   Size:       1945     758     577     496    9591

  Fragments arranged by size: 

                9591    1945     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   3299    8258    8831    9971    3299 
   Size:       4959     573    1140    6695

  Fragments arranged by size: 

                6695    4959    1140     573
 
BsiEI CG_ry'CG

Cuts at:    104   10909   10955   12163     104 
   Size:      10805      46    1208    1308

  Fragments arranged by size: 

               10805    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    734    6455    8775   10381   11159   11349   12141     734 
   Size:       5721    2320    1606     778     190     792    1960

  Fragments arranged by size: 

                5721    2320    1960    1606     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1547    1839    2025    3586    3618    5853    7569    8841    1547 
   Size:        292     186    1561      32    2235    1716    1272    6073

  Fragments arranged by size: 

                6073    2235    1716    1561    1272     292     186      32
            
 
Bsp1286I G_dGCh'C

Cuts at:    734    1326    6455    6935    7917    8389    8775    9269   10381 
   Size:        592    5129     480     982     472     386     494    1112

Cuts at:  10381   10992   11085   11159   11349   11411   12141     734 
   Size:        611      93      74     190      62     730    1960

  Fragments arranged by size: 

                5129    1960    1112     982     730     611     592     494
                 480     472     386     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:  12587   12587 
   Size:      13367
 
BspHI T'CATG_A

Cuts at:    774     813    2394    2456    2835    3903   12543     774 
   Size:         39    1581      62     379    1068    8640    1598

  Fragments arranged by size: 

                8640    1598    1581    1068     379      62      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    252    1828    5640    5770    6490   10417   10631   10933   11314 
   Size:       1576    3812     130     720    3927     214     302     381

Cuts at:  11314     252 
   Size:       2305

  Fragments arranged by size: 

                3927    3812    2305    1576     720     381     302     214
                 130
 
BsrBI CCG'CTC

Cuts at:     50   11659   11713   13197   13362      50 
   Size:      11609      54    1484     165      55

  Fragments arranged by size: 

               11609    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   7749    8640    9633   11279    7749 
   Size:        891     993    1646    9837

  Fragments arranged by size: 

                9837    1646     993     891
 
BsrFI r'CCGG_y

Cuts at:   9222   11365   11546   11755    9222 
   Size:       2143     181     209   10834

  Fragments arranged by size: 

               10834    2143     209     181
 
BsrGI T'GTAC_A

Cuts at:    550    8401    9967     550 
   Size:       7851    1566    3950

  Fragments arranged by size: 

                7851    3950    1566
 
BssSI C'ACGA_G

Cuts at:   6428   11638   11996    6428 
   Size:       5210     358    7799

  Fragments arranged by size: 

                7799    5210     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   9657   10744    9657 
   Size:       1087   12280
 
BstBI TT'CG_AA

Cuts at:  11728   11728 
   Size:      13367
 
BstYI r'GATC_y

Cuts at:      1     118     672    2160    7938    8304    8936    9291   10392 
   Size:        117     554    1488    5778     366     632     355    1101

Cuts at:  10392   10859   10882   11217   11463   12475   12561   12573   12919 
   Size:        467      23     335     246    1012      86      12     346

Cuts at:  12919       1 
   Size:        449

  Fragments arranged by size: 

                5778    1488    1101    1012     632     554     467     449
                 366     355     346     335     246     117      86      23
                  12
 
BstZ17I GTA'TAC

Cuts at:   7730    8474    7730 
   Size:        744   12623
 
Bsu36I CC'TnA_GG

Cuts at:   8024    8024 
   Size:      13367
 
BtgI C'CryG_G

Cuts at:   2549    2885    8960    9259    2549 
   Size:        336    6075     299    6657

  Fragments arranged by size: 

                6657    6075     336     299
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   8510   11317   11498   11661    8510 
   Size:       2807     181     163   10216

  Fragments arranged by size: 

               10216    2807     181     163
 
BtsI GCAGTG_nn'

Cuts at:   4128    8834    9716   10610    4128 
   Size:       4706     882     894    6885

  Fragments arranged by size: 

                6885    4706     894     882
 
ClaI AT'CG_AT

Cuts at:   3622    3622 
   Size:      13367
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   8651    8686    8651 
   Size:         35   13332
 
DraI TTT'AAA

Cuts at:    294     930    1695    2579    3020    6156    6498    7338   10083 
   Size:        636     765     884     441    3136     342     840    2745

Cuts at:  10083   10366     294 
   Size:        283    3295

  Fragments arranged by size: 

                3295    3136    2745     884     840     765     636     441
                 342     283
 
DraIII CAC_nnn'GTG

Cuts at:   5950    7523    5950 
   Size:       1573   11794
 
DrdI GACnn_nn'nnGTC

Cuts at:    188     452    3098    9858   11073   11931     188 
   Size:        264    2646    6760    1215     858    1624

  Fragments arranged by size: 

                6760    2646    1624    1215     858     264
 
EaeI y'GGCC_r

Cuts at:   1795    4215    8255    9809   10952   11126   11517   11544   12889 
   Size:       2420    4040    1554    1143     174     391      27    1345

Cuts at:  12889    1795 
   Size:       2273

  Fragments arranged by size: 

                4040    2420    2273    1554    1345    1143     391     174
                  27
 
EagI C'GGCC_G

Cuts at:  10952   10952 
   Size:      13367
 
EarI CTCTTCn'nnn_

Cuts at:   1511    2294    3178    3517    5562    6918    8870    9906   11390 
   Size:        783     884     339    2045    1356    1952    1036    1484

Cuts at:  11390   11600    1511 
   Size:        210    3278

  Fragments arranged by size: 

                3278    2045    1952    1484    1356    1036     884     783
                 339     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:  11575   11885   12031   13177   11575 
   Size:        310     146    1146   11765

  Fragments arranged by size: 

               11765    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:  10379   10379 
   Size:      13367
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    200     847    1037    1196    1415    1568    1727    2084    2765 
   Size:        647     190     159     219     153     159     357     681

Cuts at:   2765    3117    3821    5954    6197    6518    7022    7543    7820 
   Size:        352     704    2133     243     321     504     521     277

Cuts at:   7820    7913    9261    9466    9548    9689   11192   11624   12371 
   Size:         93    1348     205      82     141    1503     432     747

Cuts at:  12371   12639     200 
   Size:        268     928

  Fragments arranged by size: 

                2133    1503    1348     928     747     704     681     647
                 521     504     432     357     352     321     277     268
                 243     219     205     190     159     159     153     141
                  93      82
 
EcoNI CCTnn'n_nnAGG

Cuts at:   9436    9737    9436 
   Size:        301   13066
 
EcoO109I rG'GnC_Cy

Cuts at:  10506   10506 
   Size:      13367
 
EcoRI G'AATT_C

Cuts at:    921    1437    1815    4893   10059   10371     921 
   Size:        516     378    3078    5166     312    3917

  Fragments arranged by size: 

                5166    3917    3078     516     378     312
 
EcoRV GAT'ATC

Cuts at:   5392    5392 
   Size:      13367
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1441    2008    3905    6153    6640   11386    1441 
   Size:        567    1897    2248     487    4746    3422

  Fragments arranged by size: 

                4746    3422    2248    1897     567     487
 
FspI TGC'GCA

Cuts at:  11148   11148 
   Size:      13367
 
HaeII r_GCGC'y

Cuts at:  10801   11049   12071   13050   10801 
   Size:        248    1022     979   11118

  Fragments arranged by size: 

               11118    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1240    1272    1885    1917    1921    1953    2218    2250    2353 
   Size:         32     613      32       4      32     265      32     103

Cuts at:   2353    2385    2428    2460    2994    3007    3026    3039    3921 
   Size:         32      43      32     534      13      19      13     882

Cuts at:   3921    3953    5734    5766    7128    7160    8334    8366    8593 
   Size:         32    1781      32    1362      32    1174      32     227

Cuts at:   8593    8625    9747    9768    9779    9800   11209   11239   11241 
   Size:         32    1122      21      11      21    1409      30       2

Cuts at:  11241   11271    1240 
   Size:         30    3336

  Fragments arranged by size: 

                3336    1781    1409    1362    1174    1122     882     613
                 534     265     227     103      43      32      32      32
                  32      32      32      32      32      32      32      32
                  30      30      21      21      19      13      13      11
                   4       2
 
HincII GTy'rAC

Cuts at:   4334    5209    9107   10406    4334 
   Size:        875    3898    1299    7295

  Fragments arranged by size: 

                7295    3898    1299     875
 
HindIII A'AGCT_T

Cuts at:   5998    6803    7945    9643    9748    5998 
   Size:        805    1142    1698     105    9617

  Fragments arranged by size: 

                9617    1698    1142     805     105
 
HpaI GTT'AAC

Cuts at:   4334    9107    4334 
   Size:       4773    8594
 
KasI G'GCGC_C

Cuts at:  10797   11045   10797 
   Size:        248   13119
 
KpnI G_GTAC'C

Cuts at:  10387   10387 
   Size:      13367
 
MluI A'CGCG_T

Cuts at:   7611   12800    7611 
   Size:       5189    8178
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   8924   12038   12222    8924 
   Size:       3114     184   10069

  Fragments arranged by size: 

               10069    3114     184
 
MscI TGG'CCA

Cuts at:   1797    4217    8257    9811   11128    1797 
   Size:       2420    4040    1554    1317    4036

  Fragments arranged by size: 

                4040    4036    2420    1554    1317
 
MslI CAynn'nnrTG

Cuts at:   2399    4160    5030    5366    7193    9010    9665   11356   11483 
   Size:       1761     870     336    1827    1817     655    1691     127

Cuts at:  11483    2399 
   Size:       4283

  Fragments arranged by size: 

                4283    1827    1817    1761    1691     870     655     336
                 127
 
MspA1I CmG'CkG

Cuts at:    327     375    4867    7507    7725    8152   10478   10792   11152 
   Size:         48    4492    2640     218     427    2326     314     360

Cuts at:  11152   12165   12410     327 
   Size:       1013     245    1284

  Fragments arranged by size: 

                4492    2640    2326    1284    1013     427     360     314
                 245     218      48
 
NaeI GCC'GGC

Cuts at:  11548   11548 
   Size:      13367
 
NarI GG'CG_CC

Cuts at:  10798   11046   10798 
   Size:        248   13119
 
NcoI C'CATG_G

Cuts at:   2549    2885    8960    2549 
   Size:        336    6075    6956

  Fragments arranged by size: 

                6956    6075     336
 
NdeI CA'TA_TG

Cuts at:   1676    4198    7990    9458    1676 
   Size:       2522    3792    1468    5585

  Fragments arranged by size: 

                5585    3792    2522    1468
 
NgoMIV G'CCGG_C

Cuts at:  11546   11546 
   Size:      13367
 
NheI G'CTAG_C

Cuts at:   4687    4687 
   Size:      13367
 
NsiI A_TGCA'T

Cuts at:   5505    7076    9653    5505 
   Size:       1571    2577    9219

  Fragments arranged by size: 

                9219    2577    1571
 
NspI r_CATG'y

Cuts at:    559    2135    2321    4304    4847    5343    6875    9664   10420 
   Size:       1576     186    1983     543     496    1532    2789     756

Cuts at:  10420   10920   11827     559 
   Size:        500     907    2099

  Fragments arranged by size: 

                2789    2099    1983    1576    1532     907     756     543
                 500     496     186
 
PciI A'CATG_T

Cuts at:    555    2131    6871   11823     555 
   Size:       1576    4740    4952    2099

  Fragments arranged by size: 

                4952    4740    2099    1576
 
PflMI CCAn_nnn'nTGG

Cuts at:   3625    8253    3625 
   Size:       4628    8739
 
PmeI GTTT'AAAC

Cuts at:  10366   10366 
   Size:      13367
 
PmlI CAC'GTG

Cuts at:   8770    8770 
   Size:      13367
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2100    2132    7682    7714   12506   12538    2100 
   Size:         32    5550      32    4792      32    2929

  Fragments arranged by size: 

                5550    4792    2929      32      32      32
 
PsiI TTA'TAA

Cuts at:    320    1907    2171    2591    7970     320 
   Size:       1587     264     420    5379    5717

  Fragments arranged by size: 

                5717    5379    1587     420     264
 
PstI C_TGCA'G

Cuts at:    249    2246    2316    3867    5232    6487   10414   11099     249 
   Size:       1997      70    1551    1365    1255    3927     685    2517

  Fragments arranged by size: 

                3927    2517    1997    1551    1365    1255     685      70
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:      13367
 
PvuII CAG'CTG

Cuts at:    327    4867    7507    7725    8152   10792   11152     327 
   Size:       4540    2640     218     427    2640     360    2542

  Fragments arranged by size: 

                4540    2640    2640    2542     427     360     218
 
RsrII CG'GwC_CG

Cuts at:  11562   11562 
   Size:      13367
 
SacI G_AGCT'C

Cuts at:  10381   10381 
   Size:      13367
 
SalI G'TCGA_C

Cuts at:  10404   10404 
   Size:      13367
 
SapI GCTCTTCn'nnn_

Cuts at:   1511    3178    5562    8870    9906   11390   11600    1511 
   Size:       1667    2384    3308    1036    1484     210    3278

  Fragments arranged by size: 

                3308    3278    2384    1667    1484    1036     210
 
SbfI CC_TGCA'GG

Cuts at:   2246   10414    2246 
   Size:       8168    5199
 
ScaI AGT'ACT

Cuts at:   5274    8454   12884    5274 
   Size:       3180    4430    5757

  Fragments arranged by size: 

                5757    4430    3180
 
SexAI A'CCwGG_T

Cuts at:   7161    7161 
   Size:      13367
 
SfcI C'TryA_G

Cuts at:     33     245    1820    2200    2242    2312    3863    5228    5276 
   Size:        212    1575     380      42      70    1551    1365      48

Cuts at:   5276    6483    7301    7977   10410   11095   12088   12279      33 
   Size:       1207     818     676    2433     685     993     191    1121

  Fragments arranged by size: 

                2433    1575    1551    1365    1207    1121     993     818
                 685     676     380     212     191      70      48      42
            
 
SfoI GGC'GCC

Cuts at:  10799   11047   10799 
   Size:        248   13119
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:      13367
 
SmaI CCC'GGG

Cuts at:  10389   10389 
   Size:      13367
 
SmlI C'TyrA_G

Cuts at:   4292    4953    8857    9927   10522   11437   11929   12191    4292 
   Size:        661    3904    1070     595     915     492     262    5468

  Fragments arranged by size: 

                5468    3904    1070     915     661     595     492     262
            
 
SnaBI TAC'GTA

Cuts at:   6208    7733    6208 
   Size:       1525   11842
 
SpeI A'CTAG_T

Cuts at:     67    3226    4614    4765    5461    8230      67 
   Size:       3159    1388     151     696    2769    5204

  Fragments arranged by size: 

                5204    3159    2769    1388     696     151
 
SphI G_CATG'C

Cuts at:   9664   10420   10920    9664 
   Size:        756     500   12111

  Fragments arranged by size: 

               12111     756     500
 
SspI AAT'ATT

Cuts at:    347    1374    2544    3846    3999    4413    5198    7383    9612 
   Size:       1027    1170    1302     153     414     785    2185    2229

Cuts at:   9612     347 
   Size:       4102

  Fragments arranged by size: 

                4102    2229    2185    1302    1170    1027     785     414
                 153
 
StuI AGG'CCT

Cuts at:   4822    9509    4822 
   Size:       4687    8680
 
StyI C'CwwG_G

Cuts at:   1259    2432    2549    2885    3257    8960    9917   10009   10501 
   Size:       1173     117     336     372    5703     957      92     492

Cuts at:  10501   10855    1259 
   Size:        354    3771

  Fragments arranged by size: 

                5703    3771    1173     957     492     372     354     336
                 117      92
 
SwaI ATTT'AAAT

Cuts at:   6498    6498 
   Size:      13367
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    726    2339    8267   10053   10950   11083   11542   11751     726 
   Size:       1613    5928    1786     897     133     459     209    2342

  Fragments arranged by size: 

                5928    2342    1786    1613     897     459     209     133
            
 
TatI w'GTAC_w

Cuts at:    550    2328    3526    4811    5022    5272    6283    8401    8452 
   Size:       1778    1198    1285     211     250    1011    2118      51

Cuts at:   8452    9700    9967   10264   12882     550 
   Size:       1248     267     297    2618    1035

  Fragments arranged by size: 

                2618    2118    1778    1285    1248    1198    1035    1011
                 297     267     250     211      51
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    653     685    2239    2271    5634    5666    6602    6634   12884 
   Size:         32    1554      32    3363      32     936      32    6250

Cuts at:  12884   12916     653 
   Size:         32    1104

  Fragments arranged by size: 

                6250    3363    1554    1104     936      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:  11164   11164 
   Size:      13367
 
XbaI T'CTAG_A

Cuts at:   2119    2464    5829    6400    7954   10398    2119 
   Size:        345    3365     571    1554    2444    5088

  Fragments arranged by size: 

                5088    3365    2444    1554     571     345
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   5914   10862    5914 
   Size:       4948    8419
 
XmaI C'CCGG_G

Cuts at:  10387   10387 
   Size:      13367
 
XmnI GAAnn'nnTTC

Cuts at:   2046    3141    5142    2046 
   Size:       1095    2001   10271

  Fragments arranged by size: 

               10271    2001    1095
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NsiI     NspI
     PciI    PflMI     PmeI     PmlI     PpiI     PsiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     ApaI     AscI    AvrII     BglI    BmgBI    BsiWI
    BsmBI   BssHII   BstEII    BstXI     FseI    FspAI     MfeI     NotI
     NruI     PacI     PasI     PfoI    PpuMI    PshAI   PspOMI    PspXI
     PsrI    SacII    SanDI     SfiI    SgrAI     SrfI     XhoI     ZraI
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