Restriction map for pF1KE2630
 (Linear) (Six-base) MAPSORT of: KIEE2630.seq  Check: 7557  from: 1  to: 2457



KIEE2630.orf

 With 182 enzymes: SgfI * 

                        June 3, 2018 14:24  ..

AclI AA'CG_TT

Cuts at:      0    1072    2457 
   Size:       1072    1385
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3     574     652    1804    2457 
   Size:          3     571      78    1152     653

  Fragments arranged by size: 

                1152     653     571      78       3
 
AfeI AGC'GCT

Cuts at:      0     935    2457 
   Size:        935    1522
 
AflIII A'CryG_T

Cuts at:      0     411     878    2457 
   Size:        411     467    1579

  Fragments arranged by size: 

                1579     467     411
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2370    2457 
   Size:       2370      87
 
AleI CACnn'nnGTG

Cuts at:      0     976    2457 
   Size:        976    1481
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     286    2457 
   Size:        286    2171
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1948    1980    2457 
   Size:       1948      32     477

  Fragments arranged by size: 

                1948     477      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     712    2034    2313    2457 
   Size:        712    1322     279     144

  Fragments arranged by size: 

                1322     712     279     144
 
ApoI r'AATT_y

Cuts at:      0    1111    1547    2247    2457 
   Size:       1111     436     700     210

  Fragments arranged by size: 

                1111     700     436     210
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2014    2047    2457 
   Size:       2014      33     410

  Fragments arranged by size: 

                2014     410      33
 
BanI G'GyrC_C

Cuts at:      0      84    2457 
   Size:         84    2373
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     477    2457 
   Size:        477    1980
 
BbvCI CC'TCA_GC

Cuts at:      0    1365    1982    2457 
   Size:       1365     617     475

  Fragments arranged by size: 

                1365     617     475
 
BclI T'GATC_A

Cuts at:      0    2078    2457 
   Size:       2078     379
 
BglI GCCn_nnn'nGGC

Cuts at:      0      22     904    2457 
   Size:         22     882    1553

  Fragments arranged by size: 

                1553     882      22
 
Bme1580I G_kGCm'C

Cuts at:      0    2013    2457 
   Size:       2013     444
 
BmgBI CAC'GTC

Cuts at:      0     396    1935    2457 
   Size:        396    1539     522

  Fragments arranged by size: 

                1539     522     396
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1792    2457 
   Size:       1792     665
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75    1218    1234    1514    2457 
   Size:         75    1143      16     280     943

  Fragments arranged by size: 

                1143     943     280      75      16
 
Bpu10I CC'TnA_GC

Cuts at:      0    1365    1700    1982    2457 
   Size:       1365     335     282     475

  Fragments arranged by size: 

                1365     475     335     282
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     373     976    2457 
   Size:        373     603    1481

  Fragments arranged by size: 

                1481     603     373
 
BsaAI yAC'GTr

Cuts at:      0     375     879    2457 
   Size:        375     504    1578

  Fragments arranged by size: 

                1578     504     375
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     189    1593    2457 
   Size:        189    1404     864

  Fragments arranged by size: 

                1404     864     189
 
BseYI C'CCAG_C

Cuts at:      0     458     775     936     964    2047    2184    2457 
   Size:        458     317     161      28    1083     137     273

  Fragments arranged by size: 

                1083     458     317     273     161     137      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292    1435    2020    2171    2457 
   Size:        292    1143     585     151     286

  Fragments arranged by size: 

                1143     585     292     286     151
 
BsiEI CG_ry'CG

Cuts at:      0    2055    2457 
   Size:       2055     402
 
BsiHKAI G_wGCw'C

Cuts at:      0    1652    2457 
   Size:       1652     805
 
BsmI GAATG_Cn'

Cuts at:      0    2235    2457 
   Size:       2235     222
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1855    2457 
   Size:       1855     602
 
Bsp1286I G_dGCh'C

Cuts at:      0    1652    2013    2457 
   Size:       1652     361     444

  Fragments arranged by size: 

                1652     444     361
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1407    2042    2457 
   Size:       1407     635     415

  Fragments arranged by size: 

                1407     635     415
 
BsrBI CCG'CTC

Cuts at:      0    1689    2457 
   Size:       1689     768
 
BsrDI GCAATG_nn'

Cuts at:      0    1700    2457 
   Size:       1700     757
 
BsrFI r'CCGG_y

Cuts at:      0    1873    2457 
   Size:       1873     584
 
BsrGI T'GTAC_A

Cuts at:      0    1319    2457 
   Size:       1319    1138
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2034    2457 
   Size:       2034     423
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     243     523    1062    1552    1581    2457 
   Size:        243     280     539     490      29     876

  Fragments arranged by size: 

                 876     539     490     280     243      29
 
BstYI r'GATC_y

Cuts at:      0    2058    2457 
   Size:       2058     399
 
Bsu36I CC'TnA_GG

Cuts at:      0    2318    2457 
   Size:       2318     139
 
BtgI C'CryG_G

Cuts at:      0      99     312    1055    1994    2457 
   Size:         99     213     743     939     463

  Fragments arranged by size: 

                 939     743     463     213      99
 
BtsI GCAGTG_nn'

Cuts at:      0    1385    1586    2213    2457 
   Size:       1385     201     627     244

  Fragments arranged by size: 

                1385     627     244     201
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     127    2457 
   Size:        127    2330
 
EaeI y'GGCC_r

Cuts at:      0     152     681    1007    1457    1709    1745    1875    2052 
   Size:        152     529     326     450     252      36     130     177

Cuts at:   2052    2457 
   Size:        405

  Fragments arranged by size: 

                 529     450     405     326     252     177     152     130
                  36
 
EagI C'GGCC_G

Cuts at:      0    2052    2457 
   Size:       2052     405
 
EarI CTCTTCn'nnn_

Cuts at:      0     949    1546    2174    2457 
   Size:        949     597     628     283

  Fragments arranged by size: 

                 949     628     597     283
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3      75     574     652    1218    1234    1514    1804 
   Size:          3      72     499      78     566      16     280     290

Cuts at:   1804    2457 
   Size:        653

  Fragments arranged by size: 

                 653     566     499     290     280      78      72      16
                   3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     166    2457 
   Size:        166    2291
 
EcoO109I rG'GnC_Cy

Cuts at:      0     663    1776    2133    2457 
   Size:        663    1113     357     324

  Fragments arranged by size: 

                1113     663     357     324
 
EcoRI G'AATT_C

Cuts at:      0    2247    2457 
   Size:       2247     210
 
FspI TGC'GCA

Cuts at:      0     222    2457 
   Size:        222    2235
 
HaeII r_GCGC'y

Cuts at:      0      82      98     552     937    2457 
   Size:         82      16     454     385    1520

  Fragments arranged by size: 

                1520     454     385      82      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     467     499     711     743    1828    1860    2356    2388 
   Size:        467      32     212      32    1085      32     496      32

Cuts at:   2388    2457 
   Size:         69

  Fragments arranged by size: 

                1085     496     467     212      69      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     990    2457 
   Size:        990    1467
 
MscI TGG'CCA

Cuts at:      0     154    1459    1711    2457 
   Size:        154    1305     252     746

  Fragments arranged by size: 

                1305     746     252     154
 
MslI CAynn'nnrTG

Cuts at:      0     949     976    1556    2457 
   Size:        949      27     580     901

  Fragments arranged by size: 

                 949     901     580      27
 
MspA1I CmG'CkG

Cuts at:      0     243     775     783    1023    2051    2457 
   Size:        243     532       8     240    1028     406

  Fragments arranged by size: 

                1028     532     406     243     240       8
 
NcoI C'CATG_G

Cuts at:      0    1055    2457 
   Size:       1055    1402
 
NdeI CA'TA_TG

Cuts at:      0    2163    2457 
   Size:       2163     294
 
NspI r_CATG'y

Cuts at:      0     415    2457 
   Size:        415    2042
 
PasI CC'CwG_GG

Cuts at:      0      40    2457 
   Size:         40    2417
 
PciI A'CATG_T

Cuts at:      0     411    2457 
   Size:        411    2046
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1056    1380    1455    2457 
   Size:       1056     324      75    1002

  Fragments arranged by size: 

                1056    1002     324      75
 
PfoI T'CCnGG_A

Cuts at:      0     818     851    1103    1501    2457 
   Size:        818      33     252     398     956

  Fragments arranged by size: 

                 956     818     398     252      33
 
PmlI CAC'GTG

Cuts at:      0     375    2457 
   Size:        375    2082
 
PpuMI rG'GwC_Cy

Cuts at:      0     663    1776    2133    2457 
   Size:        663    1113     357     324

  Fragments arranged by size: 

                1113     663     357     324
 
PvuII CAG'CTG

Cuts at:      0     243     775     783    1023    2457 
   Size:        243     532       8     240    1434

  Fragments arranged by size: 

                1434     532     243     240       8
 
SanDI GG'GwC_CC

Cuts at:      0    2133    2457 
   Size:       2133     324
 
SapI GCTCTTCn'nnn_

Cuts at:      0     949    2174    2457 
   Size:        949    1225     283

  Fragments arranged by size: 

                1225     949     283
 
SexAI A'CCwGG_T

Cuts at:      0    1958    2457 
   Size:       1958     499
 
SfcI C'TryA_G

Cuts at:      0     704    2279    2457 
   Size:        704    1575     178

  Fragments arranged by size: 

                1575     704     178
 
SspI AAT'ATT

Cuts at:      0    2272    2457 
   Size:       2272     185
 
StuI AGG'CCT

Cuts at:      0     810    2457 
   Size:        810    1647
 
StyI C'CwwG_G

Cuts at:      0     498     678    1055    2457 
   Size:        498     180     377    1402

  Fragments arranged by size: 

                1402     498     377     180
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1924    2457 
   Size:       1924     533
 
TatI w'GTAC_w

Cuts at:      0    1319    2457 
   Size:       1319    1138
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1769    2457 
   Size:       1769     688
 
XmnI GAAnn'nnTTC

Cuts at:      0    1072    2457 
   Size:       1072    1385
 

 Enzymes that do cut:

     AclI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI     ApoI     BaeI     BanI     BbsI    BbvCI     BclI     BglI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     DrdI     EaeI     EagI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII     MscI     MslI
   MspA1I     NcoI     NdeI     NspI     PasI     PciI    PflMI     PfoI
     PmlI    PpuMI    PvuII    SanDI     SapI    SexAI     SfcI     SspI
     StuI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I    AflII     AgeI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI
     BcgI    BciVI    BfrBI    BglII     BlpI     BmtI     BplI    BpuEI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiWI    BspEI    BspHI   BssHII
    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     EciI  EcoICRI    EcoRV     FalI     FseI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PmeI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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