Restriction map for pF1KE1742
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MluI        MroI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
ScaI        SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        StyI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII         472  CGCG                 CG^CG
AfaI           57  GTAC                 GT^AC
Aor51HI       359  AGCGCT               AGC^GCT
ApaI          198  GGGCCC               GGGCC^C
Asp700I       549  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI           29  TGGCCA               TGG^CCA
BamHI         573  GGATCC               G^GATCC
BanI          584  GGCACC               G^G[CT][GA]CC
BclI          320  TGATCA               T^GATCA
BpmI          634  CTGGAG               CTGGAG(16/14)
BsaI          492  GAGACC               GGTCTC(1/5)
BsrDI         330  CATTGC               GCAATG(2/0)
BsrI           27  ACTGG                ACTGG(1/-1)
BstEII        279  GGTGACC              G^GT[ACGT]ACC
BstPI         279  GGTGACC              G^GT[ACGT]ACC
BstUI         472  CGCG                 CG^CG
BstXI         682  CCACTGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         573  GGATCC               [GA]^GATC[CT]
DraIII        391  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           29  TGGCCA               [CT]^GGCC[GA]
EarI          172  CTCTTC               CTCTTC(1/4)
Eco47III      359  AGCGCT               AGC^GCT
EcoO65I       279  GGTGACC              G^GT[ACGT]ACC
FbaI          320  TGATCA               T^GATCA
HaeII         359  AGCGCT               [GA]GCGC^[CT]
HinfI         465  GATTC                G^A[ACGT]TC
Ksp632I       172  CTCTTC               CTCTTC(1/4)
MflI          573  GGATCC               [GA]^GATC[CT]
MluNI          29  TGGCCA               TGG^CCA
MscI           29  TGGCCA               TGG^CCA
MspA1I        530  CAGCTG               C[AC]G^C[GT]G
MvnI          472  CGCG                 CG^CG
PvuII         530  CAGCTG               CAG^CTG
RsaI           57  GTAC                 GT^AC
SacI          169  GAGCTC               GAGCT^C
SapI          171  GCTCTTC              GCTCTTC(1/4)
SbfI          183  CCTGCAGG             CCTGCA^GG
SexAI         392  ACCAGGT              A^CC[AT]GGT
Sse8387I      183  CCTGCAGG             CCTGCA^GG
TaqI          541  TCGA                 T^CGA
TfiI          465  GATTC                G^A[AT]TC
Tsp45I        280  GTGAC                ^GT[GC]AC
Tsp509I       550  AATT                 ^AATT
TthHB8I       541  TCGA                 T^CGA
XcmI          393  CCAGGTGGCTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         573  GGATCC               [GA]^GATC[CT]
XmnI          549  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwI          574  GATCC                GGATC(4/5)
              573  GGATC               
AspHI         169  GAGCTC               G[AT]GC[AT]^C
              441  GTGCTC              
BanII         169  GAGCTC               G[GA]GC[CT]^C
              198  GGGCCC              
BsiHKAI       169  GAGCTC               G[AT]GC[AT]^C
              441  GTGCTC              
BsmAI          24  GAGAC                GTCTC(1/5)
              492  GAGAC               
BsrBI         460  GAGCGG               CCGCTC(-3/-3)
              637  GAGCGG              
CfoI          360  GCGC                 GCG^C
              702  GCGC                
DpnII         321  GATC                 ^GATC
              574  GATC                
HhaI          360  GCGC                 GCG^C
              702  GCGC                
HinP1I        360  GCGC                 G^CGC
              702  GCGC                
HphI          120  GGTGA                GGTGA(8/7)
              279  GGTGA               
MaeIII        179  GTAAC                ^GT[ACGT]AC
              280  GTGAC               
MboI          321  GATC                 ^GATC
              574  GATC                
MboII         450  GAAGA                GAAGA(8/7)
              173  TCTTC               
NdeII         321  GATC                 ^GATC
              574  GATC                
NspI          386  ACATGC               [GA]CATG^[CT]
              522  GCATGT              
Sau3AI        321  GATC                 ^GATC
              574  GATC                
TspRI         129  CACTG                CA[GC]TG[ACGT][ACGT]^
              683  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BmyI        
BsaJI       BsgI        BsiYI       BslI        Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DraII       EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HpaII       ItaI        MnlI        MslI        MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PstI        Sau96I      ScrFI       SfcI        TseI        
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