Restriction map for pF1KE1742
 (Linear) (Six-base) MAPSORT of: KIEE1742.seq  Check: 4589  from: 1  to: 732



KIEE1742.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     356     732 
   Size:        356     376
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     679     732 
   Size:        159     520      53

  Fragments arranged by size: 

                 520     159      53
 
AfeI AGC'GCT

Cuts at:      0     361     732 
   Size:        361     371
 
AleI CACnn'nnGTG

Cuts at:      0     270     433     732 
   Size:        270     163     299

  Fragments arranged by size: 

                 299     270     163
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     209     451     732 
   Size:        209     242     281

  Fragments arranged by size: 

                 281     242     209
 
ApaI G_GGCC'C

Cuts at:      0     202     732 
   Size:        202     530
 
BamHI G'GATC_C

Cuts at:      0     573     732 
   Size:        573     159
 
BanI G'GyrC_C

Cuts at:      0     584     732 
   Size:        584     148
 
BanII G_rGCy'C

Cuts at:      0     173     202     732 
   Size:        173      29     530

  Fragments arranged by size: 

                 530     173      29
 
BclI T'GATC_A

Cuts at:      0     320     732 
   Size:        320     412
 
Bme1580I G_kGCm'C

Cuts at:      0     202     732 
   Size:        202     530
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655     732 
   Size:        655      77
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     486     732 
   Size:        486     246
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     411     501     732 
   Size:        294     117      90     231

  Fragments arranged by size: 

                 294     231     117      90
 
BsiHKAI G_wGCw'C

Cuts at:      0     173     445     732 
   Size:        173     272     287

  Fragments arranged by size: 

                 287     272     173
 
Bsp1286I G_dGCh'C

Cuts at:      0     173     202     445     732 
   Size:        173      29     243     287

  Fragments arranged by size: 

                 287     243     173      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      68     191     356     732 
   Size:         68     123     165     376

  Fragments arranged by size: 

                 376     165     123      68
 
BsrBI CCG'CTC

Cuts at:      0     462     639     732 
   Size:        462     177      93

  Fragments arranged by size: 

                 462     177      93
 
BsrDI GCAATG_nn'

Cuts at:      0     329     732 
   Size:        329     403
 
BstEII G'GTnAC_C

Cuts at:      0     279     732 
   Size:        279     453
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     689     732 
   Size:        689      43
 
BstYI r'GATC_y

Cuts at:      0     573     732 
   Size:        573     159
 
DraIII CAC_nnn'GTG

Cuts at:      0     396     732 
   Size:        396     336
 
EaeI y'GGCC_r

Cuts at:      0      29     732 
   Size:         29     703
 
EarI CTCTTCn'nnn_

Cuts at:      0     178     732 
   Size:        178     554
 
EcoICRI GAG'CTC

Cuts at:      0     171     732 
   Size:        171     561
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     655     679     732 
   Size:        159     496      24      53

  Fragments arranged by size: 

                 496     159      53      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     199     630     678     732 
   Size:        199     431      48      54

  Fragments arranged by size: 

                 431     199      54      48
 
HaeII r_GCGC'y

Cuts at:      0     363     732 
   Size:        363     369
 
MscI TGG'CCA

Cuts at:      0      31     732 
   Size:         31     701
 
MslI CAynn'nnrTG

Cuts at:      0     270     433     713     732 
   Size:        270     163     280      19

  Fragments arranged by size: 

                 280     270     163      19
 
MspA1I CmG'CkG

Cuts at:      0     532     732 
   Size:        532     200
 
NspI r_CATG'y

Cuts at:      0     390     526     732 
   Size:        390     136     206

  Fragments arranged by size: 

                 390     206     136
 
PfoI T'CCnGG_A

Cuts at:      0     205     569     732 
   Size:        205     364     163

  Fragments arranged by size: 

                 364     205     163
 
PspOMI G'GGCC_C

Cuts at:      0     198     732 
   Size:        198     534
 
PstI C_TGCA'G

Cuts at:      0     188     353     668     732 
   Size:        188     165     315      64

  Fragments arranged by size: 

                 315     188     165      64
 
PvuII CAG'CTG

Cuts at:      0     532     732 
   Size:        532     200
 
SacI G_AGCT'C

Cuts at:      0     173     732 
   Size:        173     559
 
SapI GCTCTTCn'nnn_

Cuts at:      0     178     732 
   Size:        178     554
 
SbfI CC_TGCA'GG

Cuts at:      0     188     732 
   Size:        188     544
 
SexAI A'CCwGG_T

Cuts at:      0     392     732 
   Size:        392     340
 
SfcI C'TryA_G

Cuts at:      0     184     349     664     732 
   Size:        184     165     315      68

  Fragments arranged by size: 

                 315     184     165      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     260     590     732 
   Size:        260     330     142

  Fragments arranged by size: 

                 330     260     142
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     710     732 
   Size:        710      22
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     400     732 
   Size:        400     332
 
XmnI GAAnn'nnTTC

Cuts at:      0     553     732 
   Size:        553     179
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI     AlfI     ApaI    BamHI     BanI
    BanII     BclI Bme1580I     BpmI     BsaI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrDI   BstEII    BstXI    BstYI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    HaeII     MscI     MslI   MspA1I
     NspI     PfoI   PspOMI     PstI    PvuII     SacI     SapI     SbfI
    SexAI     SfcI    TaqII     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmaI     ZraI
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