################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AccIII 404 TCCGGA T^CCGGA AlwNI 730 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 466 GTGCTC G[AT]GC[AT]^C AvaII 402 GGTCC G^G[AT]CC BamHI 560 GGATCC G^GATCC BanI 751 GGCACC G^G[CT][GA]CC BsaI 382 GAGACC GGTCTC(1/5) BsaWI 404 TCCGGA [AT]^CCGG[AT] BseAI 404 TCCGGA T^CCGGA BsiHKAI 466 GTGCTC G[AT]GC[AT]^C BspEI 404 TCCGGA T^CCGGA BspMI 210 GCAGGT ACCTGC(4/8) BsrBI 594 CCGCTC CCGCTC(-3/-3) BstAPI 459 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DdeI 129 CTCAG C^T[ACGT]AG EcoNI 795 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 75 GGCGCT [GA]GCGC^[CT] HgaI 255 GACGC GACGC(5/10) MroI 404 TCCGGA T^CCGGA NcoI 200 CCATGG C^CATGG NspI 38 GCATGT [GA]CATG^[CT] PshAI 490 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 457 CTGCAG CTGCA^G SexAI 188 ACCTGGT A^CC[AT]GGT SfaNI 4 GATGC GCATC(5/9) SfiI 225 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### ApaI 233 GGGCCC GGGCC^C 655 GGGCCC BalI 95 TGGCCA TGG^CCA 423 TGGCCA BbsI 475 GTCTTC GAAGAC(2/6) 643 GTCTTC BpuAI 475 GTCTTC GAAGAC(2/6) 643 GTCTTC BsaBI 171 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 525 GATTGGGATC BsgI 48 GTGCAG GTGCAG(16/14) 208 GTGCAG BsmFI 21 GGGAC GGGAC(10/14) 766 GGGAC BstEII 192 GGTCACC G^GT[ACGT]ACC 213 GGTCACC BstF5I 375 CATCC GGATG(2/0) 3 GGATG BstPI 192 GGTCACC G^GT[ACGT]ACC 213 GGTCACC BstYI 530 GGATCT [GA]^GATC[CT] 560 GGATCC EcoO65I 192 GGTCACC G^GT[ACGT]ACC 213 GGTCACC EcoT14I 200 CCATGG C^C[AT][AT]GG 387 CCTTGG FokI 375 CATCC GGATG(9/13) 3 GGATG HinfI 585 GAATC G^A[ACGT]TC 805 GAATC MaeII 24 ACGT A^CGT 398 ACGT MamI 171 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 525 GATTGGGATC MflI 530 GGATCT [GA]^GATC[CT] 560 GGATCC MluNI 95 TGGCCA TGG^CCA 423 TGGCCA MscI 95 TGGCCA TGG^CCA 423 TGGCCA MslI 195 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 201 CATGGCTGTG PflMI 318 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 426 CCAGGCGGTGG PvuII 319 CAGCTG CAG^CTG 460 CAGCTG SfcI 166 CTACAG C^T[GA][CT]AG 457 CTGCAG StyI 200 CCATGG C^C[AT][AT]GG 387 CCTTGG TfiI 585 GAATC G^A[AT]TC 805 GAATC Van91I 318 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 426 CCAGGCGGTGG XhoII 530 GGATCT [GA]^GATC[CT] 560 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AluI AlwI BanII BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI BstXI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I TspRI