Restriction map for
pF1KE2389
(Linear) (Six-base) MAPSORT of: KIEE2389.seq Check: 1887 from: 1 to: 2073
KIEE2389.orf
With 182 enzymes: SgfI *
January 6, 2012 11:52 ..
AccI GT'mk_AC
Cuts at: 0 1871 2073
Size: 1871 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 2073
Size: 29 2044
AflIII A'CryG_T
Cuts at: 0 506 1662 1792 1825 2073
Size: 506 1156 130 33 248
Fragments arranged by size:
1156 506 248 130 33
ApoI r'AATT_y
Cuts at: 0 228 399 437 1195 1225 1597 1629 1751
Size: 228 171 38 758 30 372 32 122
Cuts at: 1751 2073
Size: 322
Fragments arranged by size:
758 372 322 228 171 122 38 32
30
AseI AT'TA_AT
Cuts at: 0 431 1927 2073
Size: 431 1496 146
Fragments arranged by size:
1496 431 146
BamHI G'GATC_C
Cuts at: 0 262 2073
Size: 262 1811
BanI G'GyrC_C
Cuts at: 0 1654 2073
Size: 1654 419
BanII G_rGCy'C
Cuts at: 0 1779 2073
Size: 1779 294
BbsI GAAGACnn'nnnn_
Cuts at: 0 1068 1877 2073
Size: 1068 809 196
Fragments arranged by size:
1068 809 196
BfrBI ATG'CAT
Cuts at: 0 1972 2073
Size: 1972 101
BglII A'GATC_T
Cuts at: 0 720 2073
Size: 720 1353
BmrI ACTGGGnnnn_n'
Cuts at: 0 1723 2073
Size: 1723 350
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 201 2073
Size: 201 1872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 2073
Size: 1519 554
BsaI GGTCTCn'nnnn_
Cuts at: 0 1537 2073
Size: 1537 536
BsaAI yAC'GTr
Cuts at: 0 702 1086 1828 2073
Size: 702 384 742 245
Fragments arranged by size:
742 702 384 245
BseYI C'CCAG_C
Cuts at: 0 2047 2073
Size: 2047 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1628 2073
Size: 1628 445
BsiHKAI G_wGCw'C
Cuts at: 0 1743 2073
Size: 1743 330
BsmI GAATG_Cn'
Cuts at: 0 642 1573 2073
Size: 642 931 500
Fragments arranged by size:
931 642 500
Bsp1286I G_dGCh'C
Cuts at: 0 1743 1779 2073
Size: 1743 36 294
Fragments arranged by size:
1743 294 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1450 2073
Size: 1450 623
BsrDI GCAATG_nn'
Cuts at: 0 78 653 2073
Size: 78 575 1420
Fragments arranged by size:
1420 575 78
BsrGI T'GTAC_A
Cuts at: 0 1588 2073
Size: 1588 485
BstYI r'GATC_y
Cuts at: 0 262 679 720 2073
Size: 262 417 41 1353
Fragments arranged by size:
1353 417 262 41
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1800 1835 2073
Size: 1800 35 238
Fragments arranged by size:
1800 238 35
DraI TTT'AAA
Cuts at: 0 488 987 2073
Size: 488 499 1086
Fragments arranged by size:
1086 499 488
DraIII CAC_nnn'GTG
Cuts at: 0 130 2073
Size: 130 1943
EarI CTCTTCn'nnn_
Cuts at: 0 192 2073
Size: 192 1881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1519 2073
Size: 29 1490 554
Fragments arranged by size:
1490 554 29
EcoRI G'AATT_C
Cuts at: 0 1751 2073
Size: 1751 322
EcoRV GAT'ATC
Cuts at: 0 182 1965 2073
Size: 182 1783 108
Fragments arranged by size:
1783 182 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 105 890 2073
Size: 105 785 1183
Fragments arranged by size:
1183 785 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1169 1201 2073
Size: 1169 32 872
Fragments arranged by size:
1169 872 32
HindIII A'AGCT_T
Cuts at: 0 213 872 2073
Size: 213 659 1201
Fragments arranged by size:
1201 659 213
MfeI C'AATT_G
Cuts at: 0 1389 2073
Size: 1389 684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 1194 2073
Size: 744 450 879
Fragments arranged by size:
879 744 450
MslI CAynn'nnrTG
Cuts at: 0 507 1355 1562 2073
Size: 507 848 207 511
Fragments arranged by size:
848 511 507 207
NsiI A_TGCA'T
Cuts at: 0 1974 2073
Size: 1974 99
NspI r_CATG'y
Cuts at: 0 510 892 1666 2073
Size: 510 382 774 407
Fragments arranged by size:
774 510 407 382
PacI TTA_AT'TAA
Cuts at: 0 431 2073
Size: 431 1642
PasI CC'CwG_GG
Cuts at: 0 675 2073
Size: 675 1398
PciI A'CATG_T
Cuts at: 0 506 1662 2073
Size: 506 1156 411
Fragments arranged by size:
1156 506 411
PflMI CCAn_nnn'nTGG
Cuts at: 0 664 1420 1728 2073
Size: 664 756 308 345
Fragments arranged by size:
756 664 345 308
PmlI CAC'GTG
Cuts at: 0 1828 2073
Size: 1828 245
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1531 1563 2073
Size: 1531 32 510
Fragments arranged by size:
1531 510 32
PstI C_TGCA'G
Cuts at: 0 1513 2073
Size: 1513 560
SfcI C'TryA_G
Cuts at: 0 1493 1509 2073
Size: 1493 16 564
Fragments arranged by size:
1493 564 16
SnaBI TAC'GTA
Cuts at: 0 1086 2073
Size: 1086 987
SspI AAT'ATT
Cuts at: 0 951 2073
Size: 951 1122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1552 2073
Size: 1552 521
TatI w'GTAC_w
Cuts at: 0 1588 2073
Size: 1588 485
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1703 1735 2073
Size: 1703 32 338
Fragments arranged by size:
1703 338 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 567 2073
Size: 567 1506
XmnI GAAnn'nnTTC
Cuts at: 0 85 873 2073
Size: 85 788 1200
Fragments arranged by size:
1200 788 85
Enzymes that do cut:
AccI AcuI AflIII ApoI AseI BamHI BanI BanII
BbsI BfrBI BglII BmrI BplI BpmI BsaI BsaAI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI
BstYI CspCI DraI DraIII EarI Eco57MI EcoRI EcoRV
FalI Hin4I HindIII MfeI MmeI MslI NsiI NspI
PacI PasI PciI PflMI PmlI PsrI PstI SfcI
SnaBI SspI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BglI BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaBI
BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MluI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI StuI StyI SwaI
Tth111I XbaI XhoI XmaI ZraI