Restriction map for
pF1KE1136
(Circular) (Six-base) MAPSORT of: pF1KE1136.seq Check: 9779 from: 1 to: 4184
pF1KE1136 4184 bp
With 182 enzymes: SgfI *
August 6, 2014 11:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 812 812
Size: 4184
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 513 2009 2441 3188 3456 200
Size: 313 1496 432 747 268 928
Fragments arranged by size:
1496 928 747 432 313 268
AflIII A'CryG_T
Cuts at: 1536 2640 3617 1536
Size: 1104 977 2103
Fragments arranged by size:
2103 1104 977
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AlwNI CAG_nnn'CTG
Cuts at: 124 1489 3056 124
Size: 1365 1567 1252
Fragments arranged by size:
1567 1365 1252
ApaI G_GGCC'C
Cuts at: 392 741 1128 392
Size: 349 387 3448
Fragments arranged by size:
3448 387 349
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 1108 1188 3537 3793 1108
Size: 80 2349 256 1499
Fragments arranged by size:
2349 1499 256 80
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 895 1204 895
Size: 309 3875
BamHI G'GATC_C
Cuts at: 413 1209 413
Size: 796 3388
BanI G'GyrC_C
Cuts at: 1200 1614 1862 1897 1200
Size: 414 248 35 3487
Fragments arranged by size:
3487 414 248 35
BanII G_rGCy'C
Cuts at: 392 741 1128 1144 1198 2228 392
Size: 349 387 16 54 1030 2348
Fragments arranged by size:
2348 1030 387 349 54 16
BbeI G_GCGC'C
Cuts at: 1618 1866 1618
Size: 248 3936
BbsI GAAGACnn'nnnn_
Cuts at: 638 638
Size: 4184
BbvCI CC'TCA_GC
Cuts at: 841 841
Size: 4184
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BglI GCCn_nnn'nGGC
Cuts at: 140 140
Size: 4184
BglII A'GATC_T
Cuts at: 1699 3736 1699
Size: 2037 2147
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 392 741 1128 1809 1902 2958 392
Size: 349 387 681 93 1056 1618
Fragments arranged by size:
1618 1056 681 387 349 93
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1477 1812 3956 1396
Size: 81 335 2144 1624
Fragments arranged by size:
2144 1624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4184
Bpu10I CC'TnA_GC
Cuts at: 841 841
Size: 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 1010 1360 2239 2731 3029 995
Size: 15 350 879 492 298 2150
Fragments arranged by size:
2150 879 492 350 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 3888 3888
Size: 4184
BsaAI yAC'GTr
Cuts at: 1537 1537
Size: 4184
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 1615 1863 2565 4061 1615
Size: 248 702 1496 1738
Fragments arranged by size:
1738 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 608 751 608
Size: 143 4041
BseYI C'CCAG_C
Cuts at: 866 1609 2367 2944 3440 866
Size: 743 758 577 496 1610
Fragments arranged by size:
1610 758 743 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 295
Size: 4184
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 385 1198 1976 2166 2958 385
Size: 813 778 190 792 1611
Fragments arranged by size:
1611 813 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 385 392 741 1128 1144 1198 1809 1902 1976
Size: 7 349 387 16 54 611 93 74
Cuts at: 1976 2166 2228 2958 385
Size: 190 62 730 1611
Fragments arranged by size:
1611 730 611 387 349 190 93 74
62 54 16 7
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 3360 3360
Size: 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 812 1234 1448 1750 2131 812
Size: 422 214 302 381 2865
Fragments arranged by size:
2865 422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 616 2476 2530 4014 4179 50
Size: 566 1860 54 1484 165 55
Fragments arranged by size:
1860 1484 566 165 55 54
BsrDI GCAATG_nn'
Cuts at: 144 633 767 2096 144
Size: 489 134 1329 2232
Fragments arranged by size:
2232 1329 489 134
BsrFI r'CCGG_y
Cuts at: 2182 2363 2572 2182
Size: 181 209 3794
Fragments arranged by size:
3794 209 181
BssSI C'ACGA_G
Cuts at: 970 2455 2813 970
Size: 1485 358 2341
Fragments arranged by size:
2341 1485 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1561 1561
Size: 4184
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 780 780
Size: 4184
BstYI r'GATC_y
Cuts at: 1 413 1209 1676 1699 2034 2280 3292 3378
Size: 412 796 467 23 335 246 1012 86
Cuts at: 3378 3390 3736 1
Size: 12 346 449
Fragments arranged by size:
1012 796 467 449 412 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 521 929 1120 107
Size: 414 408 191 3171
Fragments arranged by size:
3171 414 408 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1427
Size: 4184
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2748 1890
Size: 858 3326
EaeI y'GGCC_r
Cuts at: 209 518 1135 1769 1943 2334 2361 3706 209
Size: 309 617 634 174 391 27 1345 687
Fragments arranged by size:
1345 687 634 617 391 309 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 322 667 721 973 1031 2207 2417 322
Size: 345 54 252 58 1176 210 2089
Fragments arranged by size:
2089 1176 345 252 210 58 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 837 2392 2702 2848 3994 837
Size: 1555 310 146 1146 1027
Fragments arranged by size:
1555 1146 1027 310 146
EcoICRI GAG'CTC
Cuts at: 1196 1196
Size: 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 513 2009 2441 3188 3456 200
Size: 313 1496 432 747 268 928
Fragments arranged by size:
1496 928 747 432 313 268
EcoNI CCTnn'n_nnAGG
Cuts at: 538 538
Size: 4184
EcoO109I rG'GnC_Cy
Cuts at: 388 737 1323 388
Size: 349 586 3249
Fragments arranged by size:
3249 586 349
EcoRI G'AATT_C
Cuts at: 1108 1188 1108
Size: 80 4104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 4184
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 676 1618 1866 2888 3867 676
Size: 942 248 1022 979 993
Fragments arranged by size:
1022 993 979 942 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 498 530 2026 2056 2058 2088 498
Size: 32 1496 30 2 30 2594
Fragments arranged by size:
2594 1496 32 30 30 2
HincII GTy'rAC
Cuts at: 1223 1223
Size: 4184
HindIII A'AGCT_T
Cuts at: 182 182
Size: 4184
KasI G'GCGC_C
Cuts at: 1614 1862 1614
Size: 248 3936
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 308 2855 3039 308
Size: 2547 184 1453
Fragments arranged by size:
2547 1453 184
MscI TGG'CCA
Cuts at: 211 1945 211
Size: 1734 2450
MslI CAynn'nnrTG
Cuts at: 778 2173 2300 778
Size: 1395 127 2662
Fragments arranged by size:
2662 1395 127
MspA1I CmG'CkG
Cuts at: 1083 1295 1609 1969 2982 3227 1083
Size: 212 314 360 1013 245 2040
Fragments arranged by size:
2040 1013 360 314 245 212
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 1615 1863 1615
Size: 248 3936
NcoI C'CATG_G
Cuts at: 107 929 107
Size: 822 3362
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NspI r_CATG'y
Cuts at: 1237 1737 2644 1237
Size: 500 907 2777
Fragments arranged by size:
2777 907 500
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PflMI CCAn_nnn'nTGG
Cuts at: 866 866
Size: 4184
PfoI T'CCnGG_A
Cuts at: 539 539
Size: 4184
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PshAI GACnn'nnGTC
Cuts at: 589 589
Size: 4184
PspOMI G'GGCC_C
Cuts at: 388 737 1124 388
Size: 349 387 3448
Fragments arranged by size:
3448 387 349
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 988 1020 988
Size: 32 4152
PstI C_TGCA'G
Cuts at: 1088 1231 1916 1088
Size: 143 685 3356
Fragments arranged by size:
3356 685 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 1083 1609 1969 1083
Size: 526 360 3298
Fragments arranged by size:
3298 526 360
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 1198 1198
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 973 2207 2417 973
Size: 1234 210 2740
Fragments arranged by size:
2740 1234 210
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 157 3701 157
Size: 3544 640
SfcI C'TryA_G
Cuts at: 33 159 1084 1227 1912 2905 3096 33
Size: 126 925 143 685 993 191 1121
Fragments arranged by size:
1121 993 925 685 191 143 126
SfoI GGC'GCC
Cuts at: 1616 1864 1616
Size: 248 3936
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 1010 1025 1339 2254 2746 3008 1010
Size: 15 314 915 492 262 2186
Fragments arranged by size:
2186 915 492 314 262 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
StyI C'CwwG_G
Cuts at: 107 851 929 1318 1672 107
Size: 744 78 389 354 2619
Fragments arranged by size:
2619 744 389 354 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 768 858 1767 1900 2359 2568 316
Size: 452 90 909 133 459 209 1932
Fragments arranged by size:
1932 909 459 452 209 133 90
TatI w'GTAC_w
Cuts at: 155 3699 155
Size: 3544 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 386 418 3701 3733 386
Size: 32 3283 32 837
Fragments arranged by size:
3283 837 32 32
Tth111I GACn'n_nGTC
Cuts at: 637 1981 637
Size: 1344 2840
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1679 1679
Size: 4184
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BpmI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI